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• Inhibition of IAPs induces programmed cell death and inflammatory signaling in patient-derived metastatic breast cancer organoids

  Kaja Nicole Wächtershäuser, et al., bioRxiv - Cancer Biology 2024

  Quote: ... α-Ki67 (ab16667, Abcam), α-Ki67 (9449 ...
• ANLN directly interacts with PCNA to regulate UV induced translesion synthesis

  Bei-Bei Tong, et al., bioRxiv - Cancer Biology 2024

  Quote: ... cells were incubated overnight with antibodies against ANLN (1:1000, Abcam, ab211872) and PCNA (1:200 ...
• Loss of the tumor suppressor NUMB drives aggressive bladder cancer through hyperactivation of a RhoA/ROCK/YAP signaling circuitry

  F. A. Tucci, et al., bioRxiv - Cancer Biology 2024

  Quote: ... anti-active YAP (EPR19812) (#ab205270 from Abcam, 1:500 for IF); anti-phospho Myosin Light Chain 2 (Ser19 ...
• Structure-based Design of Chimeric Influenza Hemagglutinins to Elicit Cross-group Immunity

  Karla M. Castro, et al., bioRxiv - Biochemistry 2024

  Quote: ... Secondary incubation with anti-mouse IgG (abcam, #99617) HRP-conjugated secondary antibody diluted 1:1,500 was added and incubated for 1 hour at room temperature ...
• OST component RPN1 is a novel regulator of IRE1 RNase activity that interacts with multiple distinct IRE1 and PERK complexes

  Natacha Larburu, et al., bioRxiv - Biochemistry 2024

  Quote: The primary antibodies were purchased from abcam for BiP (ab108613) ...
• cGAS deficient mice display premature aging associated with de-repression of LINE1 elements and inflammation

  John C. Martinez, et al., bioRxiv - Cell Biology 2024

  Quote: ... H3 (1:10,000, Abcam), or ORF1p (1:500 ...
• PCDHGC3 silencing promotes clear cell renal cell carcinoma metastasis via mTOR/HIF2α and lipid metabolism reprogramming

  Lucía Celada, et al., bioRxiv - Cancer Biology 2024

  Quote: ... Membranes were probed with rabbit anti-HIF2α (ab243861, Abcam, Cambridge, UK) at 1:250 dilution ...
• Rapid kinetics of H⁺ transport by membrane pyrophosphatase: evidence for a “direct-coupling” mechanism

  Viktor A. Anashkin, et al., bioRxiv - Biochemistry 2024

  Quote: ... The gels were stained with InstantBlue Coomassie (Abcam, Cambridge, UK) and quantified using Bio-Rad Bio-Rad (Hercules ...
• Break-induced replication drives large-scale genomic amplifications in cancer cells

  Mouadh Benamar, et al., bioRxiv - Cancer Biology 2024

  Quote: ... DNA-PKcs (1:1000, ab44815; Abcam), 53BP1 (1:5000 ...
• Cancer-associated fibroblasts confer ALK inhibitor resistance in EML4-ALK-driven lung cancer via concurrent integrin and MET signaling

  Qianqian Hu, et al., bioRxiv - Cancer Biology 2024

  Quote: ... αSMA (Abcam, #ab32575), E-cadherin (Cell Signaling ...
• Development of a De Novo Protein Binder that Inhibits the Alpha Kinase eEF2K

  Kody A. Klupt, Ethan Belrose, Zongchao Jia, bioRxiv - Biochemistry 2024

  Quote: ... WST-8 assay was used according to the manufacturers protocol (Abcam ab65475) and absorbance was recorded between 0 and 48 hrs following transfection ...
• Design and Synthesis of Triazine-Based Hydrogel for Combined Targeted Doxorubicin Delivery and PI3K Inhibition

  Subhasis Mandal, et al., bioRxiv - Cancer Biology 2024

  Quote: ... 50µl of reaction buffer (containing 10mM DTT, Abcam) was added to each well ...
• TTF2 promotes replisome eviction from stalled forks in mitosis

  Geylani Can, et al., bioRxiv - Biochemistry 2024

  Quote: ... Primary antibodies: α-RNAPII-S2ph 1:400 (ab5095, rabbit, Abcam)) ...
• SFPQ-TFE3 gene fusion reciprocally regulates mTORC1 activity and induces lineage plasticity in a novel mouse model of renal tumorigenesis

  Kaushal Asrani, et al., bioRxiv - Cancer Biology 2024

  Quote: ... Melan A (#ab210546 Abcam; 1:500), PMEL (#ab137078 Abcam ...
• Mutant p53 Exploits Enhancers to Elevate Immunosuppressive Chemokine Expression and Impair Immune Checkpoint Inhibitors in Pancreatic Cancer

  Dig B. Mahat, et al., bioRxiv - Cancer Biology 2024

  Quote: ... followed by incubation overnight at 4°C with primary antibodies: 1:200 anti-CD8ɑ (Abcam), 1:250 anti-Neutrophil (Abcam) ...
• Mutant p53 Exploits Enhancers to Elevate Immunosuppressive Chemokine Expression and Impair Immune Checkpoint Inhibitors in Pancreatic Cancer

  Dig B. Mahat, et al., bioRxiv - Cancer Biology 2024

  Quote: ... 1:250 anti-Neutrophil (Abcam), and 1:100 Arginase-1 (Cell Signaling Technology) ...
• Plexin/Semaphorin Antagonism Orchestrates Collective Cell Migration, Gap Closure and Organ sculpting by Contact-Mesenchymalization

  Maik C. Bischoff, et al., bioRxiv - Cell Biology 2024

  Quote: ... and anti-GFP (Abcam, ab290, 1:1000). We used secondary Dylight secondary antibodies (ThermoFisher) ...
• Functional Impact of Nth-like DNA glycosylase on Mitochondrial Dynamics

  Lisa Hubers, et al., bioRxiv - Cell Biology 2024

  Quote: ... Cells were subsequently added a dilution series of MPP+ (Abcam, AB144783) dissolved in medium and further incubated for 72 h ...
• Mechanisms of cortical microtubule organization in epidermal keratinocytes

  Keying Guo, Andreas Merdes, bioRxiv - Cell Biology 2024

  Quote: ... rabbit anti-Cep170 (Abcam), 1:100 ...
• Annulate Lamellae biogenesis is essential for nuclear pore function

  Junyan Lin, et al., bioRxiv - Cell Biology 2024

  Quote: ... mouse monoclonal anti-PDI (Abcam, ab2792), mouse monoclonal anti-Climp63 (Enzo ...
• Annulate Lamellae biogenesis is essential for nuclear pore function

  Junyan Lin, et al., bioRxiv - Cell Biology 2024

  Quote: ... rabbit polyclonal anti-UBAP2L (Abcam, ab138309), rabbit polyclonal anti-Tubulin (Abcam ...
• Annulate Lamellae biogenesis is essential for nuclear pore function

  Junyan Lin, et al., bioRxiv - Cell Biology 2024

  Quote: ... rabbit polyclonal anti-Aurora B (Abcam, ab2254), rabbit polyclonal anti-cyclin A (H-432 ...
• Annulate Lamellae biogenesis is essential for nuclear pore function

  Junyan Lin, et al., bioRxiv - Cell Biology 2024

  Quote: ... rabbit polyclonal anti-Tubulin (Abcam, ab18251), mouse monoclonal anti-α-Tubulin (Sigma ...
• Annulate Lamellae biogenesis is essential for nuclear pore function

  Junyan Lin, et al., bioRxiv - Cell Biology 2024

  Quote: ... rabbit monoclonal anti-SUN1 (Abcam, ab124770), rabbit monoclonal anti-SUN2 (Abcam ...
• Annulate Lamellae biogenesis is essential for nuclear pore function

  Junyan Lin, et al., bioRxiv - Cell Biology 2024

  Quote: ... rabbit polyclonal anti-Nup153 (Abcam, ab84872), rabbit polyclonal anti-CRM1/Exportin 1 (Novus ...
• Annulate Lamellae biogenesis is essential for nuclear pore function

  Junyan Lin, et al., bioRxiv - Cell Biology 2024

  Quote: ... rabbit polyclonal anti-GFP (Abcam, ab290), rabbit polyclonal anti-Aurora B (Abcam ...
• Annulate Lamellae biogenesis is essential for nuclear pore function

  Junyan Lin, et al., bioRxiv - Cell Biology 2024

  Quote: ... rabbit monoclonal anti-Nesprin1 (Abcam, ab192234), rabbit polyclonal anti-Emerin (Abcam ...
• Annulate Lamellae biogenesis is essential for nuclear pore function

  Junyan Lin, et al., bioRxiv - Cell Biology 2024

  Quote: ... rabbit monoclonal anti-NTF97/Importin beta (Abcam, ab2811), mouse monoclonal anti-Ran (BD Biosciences ...
• Annulate Lamellae biogenesis is essential for nuclear pore function

  Junyan Lin, et al., bioRxiv - Cell Biology 2024

  Quote: ... mouse monoclonal anti-Nuclear Pore Complex Proteins (mAb414) (Abcam, ab24609), rabbit polyclonal anti-LC3B (Novus biological ...
• Annulate Lamellae biogenesis is essential for nuclear pore function

  Junyan Lin, et al., bioRxiv - Cell Biology 2024

  Quote: ... rabbit polyclonal anti-Nup214 (Abcam, ab70497), rabbit polyclonal anti-ELYS (Bethyl ...
• Annulate Lamellae biogenesis is essential for nuclear pore function

  Junyan Lin, et al., bioRxiv - Cell Biology 2024

  Quote: ... rabbit monoclonal anti-SUN2 (Abcam, ab124916), rabbit polyclonal anti-Lamin A (C-terminal ...
• Annulate Lamellae biogenesis is essential for nuclear pore function

  Junyan Lin, et al., bioRxiv - Cell Biology 2024

  Quote: ... rabbit polyclonal anti-Emerin (Abcam, ab40688), rabbit polyclonal anti-LAP2 (Proteintech ...
• Annulate Lamellae biogenesis is essential for nuclear pore function

  Junyan Lin, et al., bioRxiv - Cell Biology 2024

  Quote: ... rabbit polyclonal anti-Lamin B1 (Abcam, ab16048), rabbit monoclonal anti-Nesprin1 (Abcam ...
• The Two-Step Cell Death Induction by the New 2-Arachidonoyl Glycerol Analog and its Modulation by Lysophosphatidylinositol in Human Breast Cancer Cells

  Mikhail G. Akimov, et al., bioRxiv - Biochemistry 2024

  Quote: Antibodies anti-b-actin and anti-CB2 were from Abcam, Cambridge ...
• Engineered paediatric tumours retain maintains tumour genotype and phenotype for precision medicine

  MoonSun Jung, et al., bioRxiv - Cancer Biology 2024

  Quote: ... PHOX2B (abcam, #ab183741; 1:1000) and SATB2 (Cell Marque ...
• Sensitivity to Vγ9Vδ2TCR T cells is imprinted after single mutations during early oncogenesis

  Astrid Cleven, et al., bioRxiv - Cancer Biology 2024

  Quote: ... anti-RhoB rabbit polyclonal (abcam, ab170611), anti-CD3 (clone ...
• Structure of the SMYD2-PARP1 Complex Reveals Both Productive and Allosteric Modes of Peptide Binding

  Yingxue Zhang, et al., bioRxiv - Biochemistry 2024

  Quote: ... and the amount of binding was quantified using an HRP-conjugated secondary antibody (ab6721, Abcam) that generated a color signal from oxidizing the substrate 2,2′-azino-bis(3-ethylbenzthiazoline sulfonic acid) ...
• Microenvironment T-Type calcium channels regulate neuronal and glial processes to promote glioblastoma growth

  Collin J. Dube, et al., bioRxiv - Cancer Biology 2024

  Quote: ... mouse anti-Nestin (1:500, Abcam), mouse anti-Vglut1 (1:500 ...
• Structure of the SMYD2-PARP1 Complex Reveals Both Productive and Allosteric Modes of Peptide Binding

  Yingxue Zhang, et al., bioRxiv - Biochemistry 2024

  Quote: ... SMYD2 proteins were coated onto the wells and bound PARP1 was detected by the anti-PARP1 antibody (ab191217, Abcam). All binding experiments were performed in triplicate.
• Unconventional structure and function of PHD domains from Additional Sex Combs-like proteins

  CJ Reddington, AR Walsh, C Göbl, PD Mace, bioRxiv - Biochemistry 2024

  Quote: ... Three further 5-minute washes were followed by addition of a horse-radish peroxidase (HRP)-fused α-Rabbit IgG secondary antibody (Goat polyclonal, 1:5000 dilution, Abcam ab6721-1). After washing ...
• Evolution of the Spatial transcriptomic landscape during the progression of high-grade pancreatic intraductal papillary mucinous neoplasms to invasive cancer

  Sirui Peng, et al., bioRxiv - Cancer Biology 2024

  Quote: ... After Hematoxylin-eosin staining (H&E Staining Kit, Abcam, ab245880), the frozen sections were mounted on standard glass slides and reviewed by two expert pathologists to confirm the pathological diagnosis ...
• Statins attenuate Wnt/β-catenin signaling by targeting SATB family proteins in colorectal cancer

  Sneha Tripathi, et al., bioRxiv - Cancer Biology 2024

  Quote: ... SATB2 (AbCam, Cat No. Ab92447), GAPDH (AbCam ...
• A forward genetic screen identifies Sirtuin1 as a driver of neuroendocrine prostate cancer

  Francisca Nunes de Almeida, et al., bioRxiv - Cancer Biology 2024

  Quote: ... we examined the overall acetylation of proteins using anti-acetyl Lysine antibodies (Abcam ab22550) (Key Resources Table).
• Loss-of-function coding variants in the Ras of Complex Proteins/GTPase domain of Leucine Rich Repeat Kinase 2

  Sarah Butterfield, et al., bioRxiv - Biochemistry 2024

  Quote: ... rabbit-anti LRRK2 pS935 (Abcam Cat# ab133450, RRID:AB_2732035), rabbit-anti-LRRK2 pS1292 () ...
• Loss-of-function coding variants in the Ras of Complex Proteins/GTPase domain of Leucine Rich Repeat Kinase 2

  Sarah Butterfield, et al., bioRxiv - Biochemistry 2024

  Quote: ... Primary antibodies used for imaging were rabbit-anti-Rab10 pT73 (Abcam Cat# ab241060, RRID:AB_2884876) and mouse-anti-human-LAMP1 (DSHB Cat# h4a3 ...
• Loss-of-function coding variants in the Ras of Complex Proteins/GTPase domain of Leucine Rich Repeat Kinase 2

  Sarah Butterfield, et al., bioRxiv - Biochemistry 2024

  Quote: ... rabbit-anti-Rab10 (Abcam Cat# ab237703, RRID:AB_2884879), rabbit-anti-Rab12 pS106 (Abcam Cat# ab256487 ...
• Longitudinally cultured precision cut lung slices as a predictive ex vivo model for lung cancer

  Ara Sargsian, et al., bioRxiv - Cancer Biology 2024

  Quote: The extracellular LDH was measured using Lactate dehydrogenase (LDH) assay kit (fluorometric) (abcam, UK) according to the manufacturer’s instructions ...
• Longitudinally cultured precision cut lung slices as a predictive ex vivo model for lung cancer

  Ara Sargsian, et al., bioRxiv - Cancer Biology 2024

  Quote: ... Stop solution (ab210900, Abcam) was added to each section for 2min after which the sections were washed 3 times with 1x PBS ...
• Microbial lysates as low-cost serum replacements in cellular agriculture media formulation

  James Dolgin, et al., bioRxiv - Bioengineering 2024

  Quote: ... Cells were then stained using myosin heavy chain (MHC; Developmental studies hybridoma bank #MF-20, Iowa City, IA, USA) and 1:200 desmin (Abcam #ab15200, Cambridge, UK) primary antibodies diluted in blocking solution.
• Substrate Stiffness Dictates Unique Doxorubicin-induced Senescence-associated Secretory Phenotypes and Transcriptomic Signatures in Human Pulmonary Fibroblasts

  Huixun Du, et al., bioRxiv - Cell Biology 2024

  Quote: Mice lungs were fixed 48 hours in 10% buffered formalin prior to processing and Masson’s trichrome staining or immunohistochemistry with p21 primary antibody (Abcam, UK; # ab188224) (1:400 overnight at room temperature ...