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Citations for abcam :
901 - 950 of 2255 citations for S 3 Amino 4 4 cyanophenyl butanoic acid hydrochloride since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Systems Biology 2024Quote: ... Cells were incubated with the primary antibodies overnight at +4°C [anti-β III tubulin/ anti-TUBB3 (ab14545; Abcam), anti-MAP2 (ab32454)] ...
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bioRxiv - Cell Biology 2024Quote: ... samples were rinsed TBS and nuclei were counterstained with 0.5 µg/mL of 4’,6-Diamidino-2-phenylindole (DAPI; Abcam) at RT for 10 minutes ...
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bioRxiv - Bioengineering 2024Quote: ... The cells were then rinsed with PBS 1× and incubated overnight at 4 °C with rabbit anti-Neurofilament H antibody (0.6 µg/mL, ab207176, Abcam). After the primary antibody incubation ...
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bioRxiv - Cell Biology 2024Quote: ... membranes were stripped (Thermo; cat# 21059) and re-blotted with anti-alpha-actinin 4 (Abcam EPR2533[2]; rabbit monoclonal) and anti-GFP (Millipore 11811460001 ...
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bioRxiv - Cell Biology 2024Quote: ... sections were incubated overnight at 4 °C in a humidified chamber with primary antibody IL-13Rα1 (1:200, rabbit; ab79277, Abcam) and GLP-1 (1:200 ...
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bioRxiv - Cancer Biology 2024Quote: ... Primary antibodies were incubated for 16 h at 4 °C (TRAP1 Santa Cruz #sc-73604, Citrate Synthase Abcam #ab96600). Proteins were visualized using the UVITEC imaging system following incubation with horseradish peroxidase-conjugated secondary antibodies.
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bioRxiv - Cell Biology 2024Quote: ... The remaining chromatin was incubated overnight on a rotator at 4 °C with 6 μl of H3K9me3 (Abcam, ab8898) or H3K27ac (Abcam ...
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bioRxiv - Cell Biology 2024Quote: ... 12µm cryosections were fixed with 4% formaldehyde and immunostained as previously described with the following antibodies: GFP (chicken, 1:1,000; Abcam) and RFP (Rabbit ...
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bioRxiv - Cell Biology 2024Quote: ... 2 mM ATP-gamma-S Kinase substrate (Abcam, ab138911), 1 unit (100nM ...
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bioRxiv - Physiology 2020Quote: Free fatty acids were quantified using the Abcam Free Fatty Acid Quantification Kit (Abcam, ab65341), following manufacturer’s protocol.
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bioRxiv - Cell Biology 2021Quote: ... 3 μg of recombinant galectin 3(Abcam, ab89487), 1 μL of 2M UDP-GlcNAz and making the volume up to 50 μL with OGT assay buffer (50 mM Tris–HCl ...
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bioRxiv - Microbiology 2021Quote: ... dynactin 3 (DCTN 3) were purchased from Abcam Inc ...
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bioRxiv - Cancer Biology 2021Quote: ... for one hour and incubated overnight at 4°C with rabbit anti-pan-cytokeratin polyclonal antibody (Abcam ab217916 1:400). The following day ...
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bioRxiv - Molecular Biology 2020Quote: ... Fragmented DNA was re-purified using Phenol: Chloroform extraction and 4 µg of DNA was immunoprecipitated using antibodies specific against 5mC (Abcam), dsDNA (Abcam ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were fixed with ice-cold 66% ethanol overnight at 4°C and stained with propidium iodide (PI)/RNase A (Abcam) for 30 min at 37°C in the dark ...
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bioRxiv - Cell Biology 2020Quote: ... Cells attached on coverslips were fixed in 4% (w/v) formaldehyde dissolved in PBS and immunocytochemistry (ICC) was performed according to a general ICC protocol (Abcam). Permeabilization of cells was done by incubating in PBS containing 0.1% (v/v ...
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bioRxiv - Cell Biology 2020Quote: ... – diluted 1 in 100 in TBS with 1 % goat serum – was incubated overnight at 4°C with the appropriate isotype control (ab170190, Abcam). Following incubation ...
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bioRxiv - Cell Biology 2020Quote: ... the cells were incubated overnight at 4 °C with 80 μL primary antibodies diluted in PBST (anti-Ki67, dilute at 1:500, ab15580, Abcam). Later ...
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bioRxiv - Genetics 2021Quote: ... Membranes were thereafter probed with primary antibodies at 4°C for 16 hours with the following dilutions: 1:2000 anti-mCherry (ab167453, Abcam), 1:5000 anti-BiP ...
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bioRxiv - Microbiology 2020Quote: ... rabbit anti-human antibody (with the dilution of 1:1000) overnight at 4 °C and PCNA (rabbit anti-human) (abcam) and GAPDH mouse anti-human (ABclonal ...
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bioRxiv - Developmental Biology 2021Quote: ... They were then incubated overnight at 4°C with primary antibodies – chicken anti-GFP (Abcam, ab13970, used at 1/800), mouse anti-mCherry (Abcam ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... blots were incubated overnight at 4°C with the following primary antibodies in blocking buffer: Rabbit-anti-Calnexin (1:1000, ab22595, Abcam), mouse-anti-CD63 (1:3000 ...
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bioRxiv - Neuroscience 2022Quote: ... the sections were incubated overnight at 4 °C in a primary antibody solution consisting of 1:1000 rabbit-anti-PKCG (Abcam ab71558 rabbit pAB to PKCG ...
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bioRxiv - Molecular Biology 2020Quote: ... Blocking was for 30 minutes with PBST/1%BSA at 4 °C after which antibody staining was performed (anti-cyclin A2-Alexa Fluor 555, Abcam ab217731 ...
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bioRxiv - Neuroscience 2021Quote: Larvae were fixed in 4% paraformaldehyde with regions of interest labeled using the following primary antibodies: goat anti-GFP (1:200; Abcam), rabbit anti-serotonin (1:200 ...
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bioRxiv - Neuroscience 2021Quote: ... sections were incubated for 48 hours at 4°C on a shaker in primary antibody solution with 2% NHS (1:2000 Rabbit anti-C-FOS, ab208942, Abcam; 1:1000 Rat anti-Ctip2 ...
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bioRxiv - Molecular Biology 2021Quote: ... The coronal sections were then incubated for 72 hours at 4°C with primary rabbit anti-collagen IV antibody (1:200) (Abcam) in a solution with 1% BSA ...
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bioRxiv - Bioengineering 2020Quote: ... and incubated overnight with primary antibodies at 4 °C (Col I antibody: ab6308, 1:500, Abcam; Col II antibody, 1:10, Quartett Immunodiagnostika; ALP antibody, ab95462, Abcam). Afterwards ...
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bioRxiv - Cell Biology 2020Quote: ... pH 7.5) for 1 h then incubated with the appropriate diluted primary antibody at 4°C overnight: LC3 (Abcam, ab48394) 1:1000 ...
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bioRxiv - Neuroscience 2021Quote: ... and then incubated overnight at 4°C for 24 hours in 2% normal goat serum together with rabbit anti-Nrf2 at 1:500 (Abcam). Slides were then washed and incubated in the secondary antibody (1:1500 ...
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bioRxiv - Neuroscience 2020Quote: ... the cultures were fixed for 1h at room temperature in 4% paraformaldehyde and stained with mouse anti-neurofilament (RMO 270; Zymed) and rabbit anti-myc antibodies (Abcam). The number of neurofilament-positive cells was counted in 16 randomly selected frames (0.4 mm2) ...
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bioRxiv - Neuroscience 2020Quote: ... The sections were blocked with 5 % normal goat serum in Tris B for 30 minutes and incubated overnight at 4 °C with a rabbit polyclonal primary antibody against mCherry (1:3000, #167453, Abcam) diluted in blocking solution ...
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Cell Ecosystem and Signaling Pathways of Primary and Metastatic Pediatric Posterior Fossa EpendymomabioRxiv - Cancer Biology 2020Quote: ... tissue was incubated with primary antibodies overnight at 4°C at the following dilutions: goat anti-IBA1 1:500 (ab178846, Abcam), rabbit anti-DAAM2 1:50 (HPA054630 ...
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bioRxiv - Neuroscience 2020Quote: ... for 1 hour at room temperature followed by overnight incubation at 4°C with primary antibodies: 0.6 μg/mL polyclonal rabbit anti-SFPQ (ab38148, Abcam, UK), and 1:5000 monoclonal mouse anti-GAPDH (60004-1-Ig ...
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bioRxiv - Bioengineering 2021Quote: ... or H3K9me3 antibody for 30 minutes with rotation at 4°C in 100 μL of incubation buffer (ab1220 1:200, ab9045 1:200, ab8898 1:250, Abcam). Incubation buffers tested were 0.1% PBSA ...
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bioRxiv - Bioengineering 2021Quote: ... 5 x 106 beads were then incubated with an H3K9me2 antibody for 30 minutes with rotation at 4°C in 100 μL of 1% PBSA (ab1220 1:200, Abcam). Samples were then washed with 1% PBSA and incubated with secondary antibody for 10 minutes with rotation at 4°C in the dark in 100μL 0.1% PBSA (ab150107 1:250 ...
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bioRxiv - Neuroscience 2021Quote: ... blocked for 1 hr and incubated overnight at 4 °C in secondary antibodies conjugated to Alexa fluorophores (donkey anti-goat, 1:1000, Abcam).
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bioRxiv - Neuroscience 2021Quote: ... and then incubated overnight at 4°C in primary antibodies diluted in blocking solution (goat anti-ChAT, 1:200, Abcam). Fish were then washed in PBT ...
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bioRxiv - Neuroscience 2021Quote: ... This was followed by incubation for 30 min at room temperature and/or incubation overnight at 4 °C with primary antibodies (Rabbit anti-NKCC1: 59791, Abcam, 1:400 in blocking buffer ...
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bioRxiv - Cancer Biology 2020Quote: ... Samples were incubated overnight at 4°C in primary antibody mixtures (HIF1α, Biolegend; EZH2, BD Bioscience; ARL13B, ProteinTech; IMPDH2, Abcam; SMO ...
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bioRxiv - Cancer Biology 2020Quote: ... Samples were incubated overnight at 4°C for antibody crosslinking (anti-HA, ProteinTech; Normal Rabbit IgG, Cell Signalling; ARL13B, ProteinTech; and IMPDH2, Abcam) at a dilution of 1:66 ...
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bioRxiv - Neuroscience 2020Quote: ... membranes were incubated overnight at 4°C with one of the following primary antibodies: GFP (chicken, 1:2000, Abcam, #ab290), GFAP (rabbit ...
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bioRxiv - Biochemistry 2021Quote: ... The immunoprecipitation of HA-tag proteins was performed overnight in a total volume of 600 μl with 4 μg of HA-antibody (ab9110, Abcam) except the 5% of samples taken as input ...
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bioRxiv - Bioengineering 2021Quote: ... The membrane was incubated with primary antibody overnight at 4°C with collagen I (1:1000 dilution, ab34710, Abcam, Canada), tenomodulin (1:1000 ...
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bioRxiv - Cell Biology 2021Quote: ... Non-specific binding was blocked by incubating the membranes with 5% nonfat milk for 2 h at room temperature and the identity of proteins evaluated by incubation at 4 ℃ overnight with the following primary antibodies (Abcam): HSP70 (1:1000 ...
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bioRxiv - Cell Biology 2021Quote: ... 500 µg of total protein extract were used to IP with either 2.5 µg V5 rabbit polyclonal antibody or VPS35 mouse monoclonal over night at 4°C and recovered after incubating for 6h at 4°C in a rotator with equilibrated Agarose Protein-G beads (Abcam). Immunoprecipitation followed by MS analysis was performed as described but using magnetic Protein G beads (Thermo Scientific) ...
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bioRxiv - Neuroscience 2021Quote: ... The following day the slices were washed three times for 10 minutes in PBS and incubated with secondary antibodies overnight at 4°C (Goat-anti-Guinea-pig-Alexa Fluor 488, Abcam ab150185 ...
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bioRxiv - Neuroscience 2020Quote: ... The sections were then incubated overnight at 4°C with the primary antibodies (goat anti-GFP; Abcam AB5450, lot GR3319697, dilution 1:1000; rabbit anti-NeuN, Abcam AB104225 ...
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bioRxiv - Genomics 2020Quote: ... The sonicated chromatin had a size in the range of 100–500 bp and was incubated overnight at 4°C with the anti-H3K27ac antibody (#ab4729, Abcam). Samples were incubated for 1h at 4°C with Dynabeads Protein G for Immunoprecipitation (#10003D ...
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bioRxiv - Neuroscience 2020Quote: ... for 20 min at RT and then overnight at 4 °C in a humidified chamber with the following antibodies: LC3 (1:50, Abcam), p62 (1:20 ...