Labshake search
Citations for abcam :
901 - 950 of 1072 citations for 6 Amino 5 formamido uracil since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Molecular Biology 2023Quote: ... The membrane was blocked using TBS-T with 5% BSA for 1 hour at room temperature and incubated using p-SMAD3 (ab52903, Abcam), type I collagen (1310-30 ...
-
bioRxiv - Microbiology 2023Quote: ... and 12.5 uL (12.5 ug) of MHC class II antibodies in a 3:1:1 mixture of TAL-1B5 (Abcam, ab20181), EPR11226 (Abcam ...
-
bioRxiv - Genomics 2023Quote: ... The remaining supernatant was transferred to a 2 mL round-bottom tube and incubated with 5 μg/μL of anti-GFP antibody (ab290; Abcam) at 4°C with end-over-end rotation for one hour ...
-
bioRxiv - Cancer Biology 2022Quote: ... Membranes were blocked with 5% milk/TBST at room temperature for 1 hr before being incubated overnight at 4°C with paxillin (Abcam) and GAPDH (Cell Signaling Technology ...
-
bioRxiv - Pharmacology and Toxicology 2023Quote: ... 5 μL of Annexin V-FITC and 5 μL of propidium iodide were added into each tube (Annexin V-FITC Apoptosis Staining/ Detection Kit, Abcam). The tubes were incubated at RT for 5 min in the dark ...
-
bioRxiv - Cell Biology 2023Quote: ... and incubated at RT for 1hr in secondary antibody diluted in TBST-5% milk (1:4000 ab6721 Goat Anti-Rabbit IgG H&L HRP (Abcam), 1:4000 ab205719 Goat Anti-Mouse IgG H&L HRP (Abcam)) ...
-
bioRxiv - Cancer Biology 2023Quote: The 5-ethynyl-2’-deoxyuridine (EdU) incorporation assay was performed using the EdU Proliferation Assay Kit (iFluor 488) (ab219801, Abcam). Cells were seeded onto coverslips and incubated with 10 μM EdU for 4 h at 37°C in a humidified atmosphere with 5% CO2 ...
-
bioRxiv - Immunology 2023Quote: Paraffin sections (5 μm) from lung tissues were processed using standard protocols for immunohistochemistry (IHC) staining according to manufacturer’s instructions (Abcam: ab236468). Tissues were permeabilized by incubation in 0.4% Triton-X in TBS for 20 min ...
-
bioRxiv - Pathology 2023Quote: ... Sections were washed 3 times for 5-10 min in PBT and incubated for 2 h at RT in secondary antibody and Hoechst (Abcam) in 3% NDS in PBT ...
-
bioRxiv - Physiology 2023Quote: ... This was followed by placing in 5% normal goat serum for 1h and labelling for mCherry with rabbit anti-mCherry (1:10,000; Abcam; RRID:AB_2571870) and goat anti-rabbit 568 (1;400 ...
-
bioRxiv - Molecular Biology 2023Quote: ... Wells were again washed three times for 5 minutes with dPBS before incubating with secondary antibodies (goat anti-rabbit IgG Alexa Fluor 488 [Abcam] ...
-
bioRxiv - Molecular Biology 2023Quote: ... for 1 h prior to incubation in primary antibody in TBST with 5% skim-milk (rabbit anti-GFP 1:10,000; Abcam - ab290) overnight ...
-
bioRxiv - Cancer Biology 2023Quote: ... Following imaging for loading control the membrane was blocked in 5% BSA in TBS-T and incubated overnight at 4 °C using N6-methyladenosine antibody (ab151230, Abcam). The next day ...
-
bioRxiv - Biochemistry 2023Quote: ... Slides were washed in TBS (3 x 5 min) prior to incubation with biotinylated goat anti-rabbit antibodies (Abcam, ab6720) in NGS (1:200 ...
-
bioRxiv - Cancer Biology 2023Quote: ... Antigen retrieval was accomplished by a 5-minute pressure cook of the slide submerged in 1X HIER buffer (abcam ab208572). Deionized water was slowly added to the pressure-cooked slide in HIER buffer ...
-
bioRxiv - Microbiology 2023Quote: ... Serine racemase treatment of serum was performed with the addition of 5 µl of proprietary serine racemase solution from a DL-serine assay kit (Abcam) to 1 ml of serum for 3 h ...
-
bioRxiv - Biochemistry 2023Quote: ... samples were washed with 5 mL of 0.1% Triton X-100/PBS and incubated with 1 μL of IRF5 antibody (Abcam, 181553) in 100 μL of 0.1% Triton X-100/PBS for 2 h at RT ...
-
bioRxiv - Pharmacology and Toxicology 2023Quote: ... The slices were blocked with 5% goat serum (Beyotime, China) for 30 min and then incubated with anti-Aβ1-42 (1:300, Abcam, UK), anti-Tau(pSer396 ...
-
bioRxiv - Cancer Biology 2023Quote: ... Pyruvate concentration in the centrifuged extracellular medium samples (5 min 18000 g, 4°C) was measured in microplates using Pyruvate Assay kit (Abcam) or as described in 36.
-
bioRxiv - Pathology 2024Quote: ... paraffin-embedded 5 µm slices were stained for insulin (ab195956, 1:1500, Alexa Fluor 555-conjugated secondary antibody: ab150186, 1:500, Abcam) and glucagon (ab92517 ...
-
bioRxiv - Cell Biology 2023Quote: ... sections were blocked with 10% goat serum and then stained with Keratin 5 or Keratin 8 together with GFP antibody (Abcam) in 10% goat serum for overnight at 4□ ...
-
bioRxiv - Molecular Biology 2024Quote: Cells were stained 3-4 days after editing with anti-Cytokeratin 5 antibody [EP1601Y] (Alexa Fluor® 647) (Abcam, ab193895), Rabbit IgG ...
-
bioRxiv - Microbiology 2024Quote: ... 5% CO2 for 24 h before supernatants were collected and LDH levels determined using an LDH-Cytotoxicity Assay Kit (abcam).
-
bioRxiv - Neuroscience 2024Quote: ... Slides were then washed 3X in 0.4% Triton-X-100 TBS for 5 minutes before blocking at room temperature for 2 hours in a solution of 10% Normal Goat Serum (Abcam), 3% BSA (Fisher ...
-
bioRxiv - Neuroscience 2024Quote: ... Sections were then incubated overnight at 4⁰C on a plate rocker in a 5% NGS/0.1% PBS-Triton solution containing rabbit anti-cFos antibody (Abcam, ab190289) at 1:500 dilution ...
-
bioRxiv - Neuroscience 2024Quote: ... the membrane was washed in TBS-T three times for 5 minutes each and incubated with goat anti-mouse IgG HRP (1:5000, cat# AB205719, Abcam) in TBS-T for 1 h at room temperature ...
-
bioRxiv - Neuroscience 2024Quote: ... The slides were then incubated for 60 minutes at room temperature in blocking buffer (PBS with 5 % goat serum [Abcam #ab7481] and 0.3 % Triton X-100 [Bio-Rad Laboratories #161-0407]) ...
-
bioRxiv - Neuroscience 2024Quote: ... and blocked with 5% milk in TBS-Tween 20 for 2h and incubated with primary antibodies diluted in the 5% BSA in TBS-Tween 20 overnight at 4°C (AMPD2, Sigma HPA045760, 1:1000; IMPDH2, Abcam ab129165 ...
-
bioRxiv - Cell Biology 2023Quote: ... Paraffin-embedded tissue was sectioned (5 µm) and stained with control IgG or ANO1 antibodies (Abcam # ab53212, 3 µg/ml) by the University of Chicago Immunohistochemistry Core Facility.
-
bioRxiv - Cancer Biology 2023Quote: ... were sonicated and incubated overnight under rotation at 4 °C with 5 ug/ml of anti-RNAPII-Ser2p (Abcam, ab193468) or anti-IgG (Cell Signaling ...
-
bioRxiv - Biochemistry 2024Quote: ... Membranes blocked for 1 h at room temperature were probed overnight at 4 °C in 5% non-fat dry milk with 1:2,000 anti-CA (Abcam ab63917) or 1:5,000 anti-IN77 ...
-
bioRxiv - Microbiology 2024Quote: ... Cells were then permeabilized in 1mL of 0.1% Triton X-100 in 1X PBS for 5 min at room temperature and incubated with anti-DDX5 primary antibody (rabbit, ab21696; Abcam) diluted to 1:400 in PBS 1X for 1 hour ...
-
bioRxiv - Cancer Biology 2024Quote: ... The membranes were incubated with the following primary antibodies in 5% skim milk at 4 °C overnight: rabbit anti-ATF4 antibody (ab270980, 1:1000, Abcam), XBP1 antibody (ab37152 ...
-
bioRxiv - Developmental Biology 2023Quote: ... For anti-PI4KIIIalpha staining, the blocking buffer (for blocking, primary, and secondary steps) was supplemented with 5% Normal Goat Serum (Abcam).
-
bioRxiv - Microbiology 2020Quote: The mutant library was screened by preparing a 1 × 10-6 dilution in LB medium and plating on LB agar containing 60 µg ml-1 X-Gal (5-bromo-4-chloro-3-indolyl-β-D-galactopyranoside, Abcam) and 500 µM IPTG ...
-
bioRxiv - Molecular Biology 2021Quote: ... The membrane was blocked in 5% milk for 1h followed by incubation with primary antibodies: c-Myc (Abcam ab185656; 1:1000), Fos (Cell Signaling 2250S ...
-
bioRxiv - Developmental Biology 2021Quote: ... Sections were blocked with 5% donkey serum and incubated overnight at 4°C against MMP13 (1:100, Abcam ab39012, Cambridge, UK) and for 2 hours at room temperature with a Cy3-conjugated secondary (1:500 ...
-
bioRxiv - Neuroscience 2022Quote: ... then incubated for 2 d at 4°C in primary rabbit anti-TH antibody (Abcam, 1:200, in TBSMT + 5% DMSO). After 6 washes x 1 h with TBST ...
-
bioRxiv - Neuroscience 2021Quote: ... injected with vehicle (VEH = 0.9% aqueous solution of NaCl and 5% ethanol) or MTZ (10 mg/kg, ab120068, Abcam, Cambridge, UK). The 10 mg/kg daily dosage in mice is equivalent to 50 mg/day in humans ...
-
bioRxiv - Immunology 2019Quote: ... monocytes and platelets were incubated with primary specific antibodies against Annexin A1 (ANXA1; 5 μg/ml; clone 1B, in house generated) and Gelsolin (GSN; 1.54 μg/ml clone EPR1942; Abcam, Cambridge, UK) in either PBS+0.2% BSA or T-PBS+0.2% BSA overnight at 4 °C ...
-
bioRxiv - Biochemistry 2020Quote: ... Membranes were treated with antibodies diluted 1:1000 in 0.5% BSA and washed 3 times for 5 min before applying secondary antibiotic (anti-mouse or anti-goat from Santa Cruz Biotechnology and Anti-Rabbit from Abcam at a concentration of 1:1000 ...
-
bioRxiv - Neuroscience 2019Quote: ... Sections were blocked with 5% fish gelatine before incubation with the primary antibodies for myelin basic protein (MBP, Abcam, 1:500) and protein gene product 9.5 (PGP ...
-
bioRxiv - Immunology 2021Quote: ... The membrane was then washed 3×15 minutes in PBST and then incubated in secondary antibody solution (PBST + 5% BSA + Goat a-Rabbit-HRP 1:5000) (Abcam, ab205718) for one hour at room temperature ...
-
bioRxiv - Neuroscience 2020Quote: ... The blots were blocked in TBST buffer containing 5% nonfat milk and then incubated overnight at 4°C with the primary rabbit polyclonal anti-Tip60 (1:1000, abcam, #ab23886), rabbit polyclonal anti-Rpd3 (1:1000 ...
-
bioRxiv - Bioengineering 2021Quote: ... blocked with 5% goat serum and incubated overnight with primary polyclonal rabbit antibodies in 5% goat serum at 1:150 dilution for types I (Abcam AB34710), II (Abcam AB34712) ...
-
bioRxiv - Cell Biology 2021Quote: ... Afterwards membranes were blocked for 30 min with 5% Nutrilon (Nutricia) in PBS and incubated overnight at 4°C with primary antibodies H3 (Abcam 1791), H4 (Merck Millipore 04-858) ...
-
bioRxiv - Neuroscience 2019Quote: ... Blots were then blocked with 5% skim milk in PBS-T for 1 hour and incubated with either rabbit anti-lamin B1 (1:1000, Abcam, ab16048), rabbit anti-dynamin 1 (1:1000 ...
-
bioRxiv - Immunology 2021Quote: ... Cells were washed in PBS for 5 min before being blocked with 10% normal donkey serum (Abcam, ab7475, Lot GR3234297-32) diluted in PBS for 20 minutes at room temperature ...
-
bioRxiv - Immunology 2021Quote: ... Fixed cells were blocked with 5% BSA in PBS for 1 hr at room temperature and incubated with rabbit anti-mouse Ki67 primary antibody (Abcam ab15580) at 1:200 dilution overnight at 4°C ...
-
bioRxiv - Cancer Biology 2021Quote: ... Tissue samples were washed with PBS three times for 5 minutes each before adding a secondary goat anti-rabbit IgG H & L (1:1000, Alexa Fluor® 555, Abcam) and Goat Anti-Rat IgG H & L (1:1000 ...