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• Lowering glucose enhances BACE1 activity and Aβ generation in mouse brain slice cultures

  Olivia Sheppard, et al., bioRxiv - Neuroscience 2024

  Quote: ... Antibodies were used as follows: rabbit TUJ1 (Sigma T2200; 1:500 and rabbit GFAP (Abcam ab7260; 1:1000 ). After three PBS washes ...
• Lowering glucose enhances BACE1 activity and Aβ generation in mouse brain slice cultures

  Olivia Sheppard, et al., bioRxiv - Neuroscience 2024

  Quote: ... Antibodies for this procedure were rabbit APP (Abcam: ab32136; 1:1000), rabbit TUJ1 (Sigma ...
• Age- and Sex-Dependent Caveolar Gene Expression in Normoxic and Post-Ischemic Mouse Hearts

  Can J. Kiessling, bioRxiv - Molecular Biology 2024

  Quote: ... from Santa Cruz Biotechnology (Dallas, TX, USA), and Cavin-1 (ab48824, rabbit polyclonal) from Abcam (Cambridge, MA, USA). Samples were prepared in fresh Laemmli buffer containing 30 μg of protein from whole-cell fractions and loaded onto 12% acrylamide gels ...
• Leukemia aggressiveness is driven by chromatin remodeling and expression changes of core regulators

  Gracia Bonilla, et al., bioRxiv - Molecular Biology 2024

  Quote: ... H3K27ac (Abcam ab4729). Samples were further processed for high throughput sequencing ...
• Leukemia aggressiveness is driven by chromatin remodeling and expression changes of core regulators

  Gracia Bonilla, et al., bioRxiv - Molecular Biology 2024

  Quote: ... Antibodies used: H3K4me1 (Abcam ab8895), H3K4me3 (Millipore ...
• Cholinergic modulation of interhemispheric inhibition in the mouse motor cortex

  Takashi Handa, Qing Zhang, Hidenori Aizawa, bioRxiv - Neuroscience 2024

  Quote: ... Sections were incubated with monoclonal rabbit anti-parvalbumin (PV) antibody (1:500, ab181086, Abcam) and rat anti-muscarinic ACh receptor m2 (M2R ...
• Cholinergic modulation of interhemispheric inhibition in the mouse motor cortex

  Takashi Handa, Qing Zhang, Hidenori Aizawa, bioRxiv - Neuroscience 2024

  Quote: ... including donkey anti-rabbit IgG-Alexa Fluor 488 (1:500, ab150073, Abcam) and goat anti-rat IgG-Alexa Fluor 594 (1:500 ...
• CD47 is Required for Mesenchymal Progenitor Proliferation and Fracture Repair

  Robert L. Zondervan, et al., bioRxiv - Molecular Biology 2024

  Quote: ... Immunofluorescence staining was performed on 5 µm sagittal sections using rabbit anti-mouse CD31(Abcam, ab281583) and rat anti-mouse Emcn primary antibodies to label endothelial cells ...
• Dispensable regulation of brain development and myelination by Serpina3n

  Meina Zhu, et al., bioRxiv - Neuroscience 2024

  Quote: ... Rabbit anti-Sox10 (1:200, Abcam, Cat# ab155279, RRID:AB_2650603); Rabbit anti-TCF4/TCF7L2 (1:200 ...
• Nandrolone alters the behavioral response to cocaine as well as striatal and cortical dopamine receptors of prepubertal male rats

  Jaime A. Freire-Arvelo, et al., bioRxiv - Neuroscience 2024

  Quote: ... and a B-Actin antibody (1:2500; Abcam, MA, #ab8227) dissolved in Odyssey Blocking Buffer ...
• Sub-clinical glutamate receptor antagonist combinations prevent progressive demyelination

  Verity F. Mitchener, et al., bioRxiv - Neuroscience 2024

  Quote: ... coli LPS (1:200 in 2% BSA and 0.1% Triton X-100 [TBS]) (Abcam; ab35654), washed and incubated in horse anti-mouse biotinylated secondary (1:200 in 2% BSA and 0.1% Triton {TBS] ...
• Reversal of neuronal tau pathology, metabolic dysfunction, and electrophysiological defects via adiponectin pathway-dependent AMPK activation

  Eric R. McGregor, et al., bioRxiv - Neuroscience 2024

  Quote: ... LC3b (ab48394; Abcam), p62 (23214 ...
• Neuronal potassium channel activity triggers initiation of mRNA translation through binding of translation regulators

  Taylor J. Malone, et al., bioRxiv - Neuroscience 2024

  Quote: ... anti-eIF4E 1:1000 (Abcam, ab33766), anti-GAPDH 1:1000 (Santa Cruz ...
• AQP1 Differentially Orchestrates Endothelial Cell Senescence

  Khatereh Shabanian, et al., bioRxiv - Molecular Biology 2024

  Quote: ... and total-HDAC4 (1:1000; ab12172) were obtained from abcam; antibody against CD31 (1:50 ...
• Perturbing nuclear glycosylation in the mouse preimplantation embryo slows down embryonic growth

  Sara Formichetti, et al., bioRxiv - Developmental Biology 2024

  Quote: ... anti-O-GlcNAc clone RL2 (abcam #ab2739 and Merck Millipore #MABS157), anti-O-GlcNAc clone HGAC85 (ThermoFisher #HGAC85) ...
• Perturbing nuclear glycosylation in the mouse preimplantation embryo slows down embryonic growth

  Sara Formichetti, et al., bioRxiv - Developmental Biology 2024

  Quote: The primary antibodies used were: anti-OGT (abcam #ab177941), anti-OGA (ThermoFisher #PA5-67426) ...
• Perturbing nuclear glycosylation in the mouse preimplantation embryo slows down embryonic growth

  Sara Formichetti, et al., bioRxiv - Developmental Biology 2024

  Quote: ... anti-CDX2 (abcam #ab76541). Dilution of all primary antibodies was 1:200 ...
• Age-dependent regulation of axoglial interactions and behavior by oligodendrocyte AnkyrinG

  Xiaoyun Ding, et al., bioRxiv - Neuroscience 2024

  Quote: ... MBP (Abcam Cat# ab40390, RRID:AB_1141521, 1:500), and Calbindin D-28K (Millipore Cat# AB1778 ...
• Age-dependent regulation of axoglial interactions and behavior by oligodendrocyte AnkyrinG

  Xiaoyun Ding, et al., bioRxiv - Neuroscience 2024

  Quote: The following primary antibodies were used for immunoblotting: Rabbit polyclonal antibodies against Caspr (Abcam Cat# ab34151, RRID:AB_869934), Calbindin D-28K (Millipore Cat# AB1778 ...
• Age-dependent regulation of axoglial interactions and behavior by oligodendrocyte AnkyrinG

  Xiaoyun Ding, et al., bioRxiv - Neuroscience 2024

  Quote: ... Chicken polyclonal antibodies against GFAP (Abcam Cat# ab134436, RRID:AB_2818977, 1:2000) and Neurofascin (R&D Systems ...
• Age-dependent regulation of axoglial interactions and behavior by oligodendrocyte AnkyrinG

  Xiaoyun Ding, et al., bioRxiv - Neuroscience 2024

  Quote: ... Rabbit polyclonal antibodies against Caspr (Abcam Cat# ab34151, RRID:AB_869934, 1:500), MBP (Abcam Cat# ab40390 ...
• Role of Fibronectin in Postnatal Skeletal Development

  Neha E. H. Dinesh, et al., bioRxiv - Developmental Biology 2024

  Quote: ... 6-diamidino-2-phenylindole (DAPI) (Abcam; Catalog #188804) was used to counterstain cell nuclei ...
• Prevotella are major contributors of sialidases in the human vaginal microbiome

  Paula Pelayo, et al., bioRxiv - Microbiology 2024

  Quote: ... and stained with InstantBlue Coomassie protein stain (Abcam, ab119211). The protein-containing fractions were pooled and concentrated in an Amicon spin concentrator of 10-30kDa cutoff (Millipore ...
• Interplay between ß-propeller subunits WDR26 and muskelin regulates the CTLH E3 ligase supramolecular complex

  Matthew E.R. Maitland, et al., bioRxiv - Molecular Biology 2024

  Quote: ... WDR26 (ab85962, Abcam); RanBP9 (5M ...
• Effective Gene Therapy for Metachromatic Leukodystrophy Achieved with Minimal Lentiviral Genomic Integrations

  Lucas Tricoli, et al., bioRxiv - Molecular Biology 2024

  Quote: ... Syntenin-1 (Abcam, Waltham, MA) CD81 (Santa Cruz ...
• Synphilin-1 as a modulator of aSyn assembly

  Diana F. Lázaro, et al., bioRxiv - Molecular Biology 2024

  Quote: ... MJFR-14-6-4-2-conformation specific (ab209538, Abcam) and 5G4-aggregated aSyn (MABN389 ...
• Identification of markers for the isolation of neuron-specific extracellular vesicles

  Dmitry Ter-Ovanesyan, et al., bioRxiv - Molecular Biology 2024

  Quote: ... 9F9.F9 for GFP (Abcam), 1.3.3.22 (ThermoFisher Scientific ...
• Identification of markers for the isolation of neuron-specific extracellular vesicles

  Dmitry Ter-Ovanesyan, et al., bioRxiv - Molecular Biology 2024

  Quote: ... and mCherry (clone EPR20579, Abcam). The next day ...
• Quinoline-based compounds can inhibit diverse enzymes that act on DNA

  Jujun Zhou, et al., bioRxiv - Molecular Biology 2024

  Quote: ... phosphor p53 (Ser15) (Abcam, ab1431), p21 (BD Biosciences ...
• Identification of markers for the isolation of neuron-specific extracellular vesicles

  Dmitry Ter-Ovanesyan, et al., bioRxiv - Molecular Biology 2024

  Quote: ... and Isotype Control (AB37385, Abcam). To couple sheep antibody to beads ...
• Quinoline-based compounds can inhibit diverse enzymes that act on DNA

  Jujun Zhou, et al., bioRxiv - Molecular Biology 2024

  Quote: ... The secondary antibodies used were: HRP-conjugated anti-rabbit-IgG (CST, #7074) and HRP-conjugated anti-mouse-IgG (Abcam, ab6820).
• Identification of markers for the isolation of neuron-specific extracellular vesicles

  Dmitry Ter-Ovanesyan, et al., bioRxiv - Molecular Biology 2024

  Quote: ... EPR18998 for L1CAM (Abcam) at 1:500 ...
• Identification of markers for the isolation of neuron-specific extracellular vesicles

  Dmitry Ter-Ovanesyan, et al., bioRxiv - Molecular Biology 2024

  Quote: ... 1C51 for mCherry (Abcam), 9F9.F9 for GFP (Abcam) ...
• The SAGA acetyltransferase module is required for the maintenance of MAF and MYC oncogenic gene expression programs in multiple myeloma

  Ying-Jiun C. Chen, et al., bioRxiv - Molecular Biology 2024

  Quote: ... anti-H3 (Abcam, 1:20,000), anti-GAPDH (Millipore ...
• SARM1 activation induces reversible mitochondrial dysfunction and can be prevented in human neurons by antisense oligonucleotides

  Andrea Loreto, et al., bioRxiv - Neuroscience 2024

  Quote: ... blocked for 1 hour with 10% serum in PBS and incubated overnight at 4LC with the following primary antibodies in PBS: chicken anti-MAP2 (Abcam, ab92434, 1:500), rabbit anti- tyrosine hydroxylase (TH ...
• The BLM-TOP3A-RMI1-RMI2 proximity map reveals that RAD54L2 suppresses sister chromatid exchanges

  Jung Jennifer Ho, et al., bioRxiv - Molecular Biology 2024

  Quote: ... anti-ABRAXAS2 (Abcam #ab68801; 1:1000), anti-RAD54L2 (ABclonal #A6144 ...
• The SAGA acetyltransferase module is required for the maintenance of MAF and MYC oncogenic gene expression programs in multiple myeloma

  Ying-Jiun C. Chen, et al., bioRxiv - Molecular Biology 2024

  Quote: ... The following antibodies were used: anti-ADA2B (Abcam, 1:1000 (discontinued) or Santa Cruz ...
• The SAGA acetyltransferase module is required for the maintenance of MAF and MYC oncogenic gene expression programs in multiple myeloma

  Ying-Jiun C. Chen, et al., bioRxiv - Molecular Biology 2024

  Quote: ... anti-MAF (Abcam, 1:1000), anti-E2F1 (Santa Cruz ...
• The SAGA acetyltransferase module is required for the maintenance of MAF and MYC oncogenic gene expression programs in multiple myeloma

  Ying-Jiun C. Chen, et al., bioRxiv - Molecular Biology 2024

  Quote: ... anti-H3K9ac (Abcam, 1:1000), anti-H3 (Abcam ...
• The SAGA acetyltransferase module is required for the maintenance of MAF and MYC oncogenic gene expression programs in multiple myeloma

  Ying-Jiun C. Chen, et al., bioRxiv - Molecular Biology 2024

  Quote: ... anti-USP22 (Abcam, 1:2000), anti-MYC (Cell Signaling Technology ...
• Axolotl mandible regeneration occurs through mechanical gap closure and a shared regenerative program with the limb

  Julia Kramer, et al., bioRxiv - Developmental Biology 2024

  Quote: ... incubated with Hoechst 33258 (Abcam Ab228550) 1:1000 in PBS 1X for 10 min ...
• A small molecule inhibitor Mirin prevents TOP3A-dependent mtDNA breakage and segregation

  Koit Aasumets, et al., bioRxiv - Molecular Biology 2024

  Quote: ... mouse anti-TFAM (1:10000; ab119684; Abcam), rabbit anti-TOMM40 (1:1000 ...
• Extended longevity of termite kings and queens is accompanied by extranuclear localization of telomerase in somatic organs and caste-specific expression of its isoforms

  Marie Pangrácová, et al., bioRxiv - Molecular Biology 2024

  Quote: ... secondary antibody horseradish peroxidase (HRP)-conjugated Goat Anti-Rabbit IgG (1:5000; Abcam), both in blocking solution and detection using the Supersensitive TMB substrate (3,3’,5,5’-tetramethylbenzidine ...
• The ER-phagy receptor FAM134B is targeted by Salmonella Typhimurium to promote infection

  Damián Gatica, et al., bioRxiv - Molecular Biology 2024

  Quote: ... and Anti-S6K (Cat#ab32529) were obtained from Abcam. Anti-RFP (Cat# 600-401-379 ...
• Rejuvenation of aged oocyte through exposure to young follicular microenvironment

  HaiYang Wang, et al., bioRxiv - Developmental Biology 2024

  Quote: ... The aRCFs were then encapsulated in Alg-rBM IPN and cultured either with or without the gap junction blocker Carbenoxolone (ab143590, Abcam) for 3 hours ...
• Rejuvenation of aged oocyte through exposure to young follicular microenvironment

  HaiYang Wang, et al., bioRxiv - Developmental Biology 2024

  Quote: ... rabbit anti-N Cadherin antibody (ab18203, Abcam; 1:100), rabbit anti-Rec8 antibody43 (a gift from Lampson Lab) ...
• APOE regulates the transport of GM1

  Dong Yan Zhang, et al., bioRxiv - Neuroscience 2024

  Quote: ... anti-ApoER2 antibody (diluted 1:1000, Abcam), anti-VLDLR antibody produced in rabbit (diluted 1:1000 ...
• Origin and adult renewal of the gut lacteal musculature from villus myofibroblasts

  Bhargav D. Sanketi, et al., bioRxiv - Developmental Biology 2024

  Quote: ... FOXP2 (1:250, Abcam, ab16046), NOTCH3 (Abcam ...
• Tcf12 controls dynamic calvarial bone growth and motor learning in mice

  Takahiko Yamada, et al., bioRxiv - Developmental Biology 2024

  Quote: ... anti-Sp7 (Abcam ab22552, 1:100), or anti-Ki67 (Abcam ab15580 ...
• Interneuron-like features of microglia in the mouse outer retina

  Sara Toros, et al., bioRxiv - Neuroscience 2024

  Quote: ... rabbit anti-Calbindin (Abcam, ab108404), mouse anti-calretinin (Sigma ...