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Citations for abcam :
851 - 900 of 10000+ citations for SUN domain containing protein 2 SUN2 Antibody since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... Immunoprecipitated proteins were separated by SDS-PAGE and subjected to Western blotting with antibody against Syx phospho-S14 (Abcam; ab63571). Digitized scans were derived by PhosphorImager (Molecular Dynamics ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... mCherry was identified based on its auto fluorescent signal while the luciferase protein was immuno-stained with anti-firefly luciferase antibody (Abcam) labeled with Zenon AF647 Mouse Labeling Kit (Life Technology) ...
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bioRxiv - Biochemistry 2021Quote: ... Clones were validated for GPC1/5 knockouts via Western analysis following heparinase III digestion using a monoclonal antibody (3G10) direct against the glycosylation attachment site in the core protein structure (370260, abcam) or via sequencing (GPC1 ...
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bioRxiv - Cell Biology 2021Quote: ... The co-immunoprecipitated protein complexes were detected using western blotting with the following antibodies: anti-TCFL5 (1:1000, ab188075, Abcam), anti-FXR1 (1:1000 ...
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bioRxiv - Cell Biology 2021Quote: ... Proteins were transferred onto Hybond nitrocellulose membranes (Amersham biosciences) and probed with antibodies specific for alpha smooth muscle actin (Abcam), CLIC4 (Santa Cruz) ...
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bioRxiv - Pathology 2023Quote: ... The expression of the MMP2 proteins in cell lysate and culture medium were confirmed by western blot analyses using antibody against MMP2 (ab92536, Abcam).
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bioRxiv - Pathology 2023Quote: ... Five micrograms of extracted protein was used for western blot analysis with primary antibody against MMP2 (1:200; ab92536, Abcam). The signal intensity of bands was quantified using the Image J densitometry software (NIH).
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bioRxiv - Cancer Biology 2023Quote: ... 20 µl of protein A magnetic beads pre adsorbed with 0.5 µg of anti-lamin A/C antibody (Abcam, #ab108595) were added to the protein mixture and incubated for 2 hours at RT ...
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bioRxiv - Physiology 2022Quote: ... Samples were transferred to PVDF membranes that were subsequently probed with the following antibodies for protein and phosphoprotein detection: anti-PIK3CA (ab40776, Abcam), anti-PDPK1 (ab52893 ...
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bioRxiv - Biochemistry 2023Quote: ... The tri-methylated lysine 9 and 27 analogs on H3K9C/C110A and H3K27C/C110A proteins were confirmed by western blot assays using monoclonal anti-Histone H3K9me3 and H3K27me3 antibody (Abcam).
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bioRxiv - Neuroscience 2023Quote: ... cells were stained for 10 minutes at 37°C with a chicken anti-green fluorescent protein (GFP) antibody (cross-reactive with YFP; ab13970, AbCam) diluted 1:1000 in neurobasal medium to stain for surface GluN2B ...
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bioRxiv - Microbiology 2024Quote: ... Pan-flavivirus envelope protein antibody 4G2 was conjugated to APC using Lightning-Link® APC Conjugation Kit (abcam, Cat# ab201807). Cells were then blocked with Human TruStain FcX in FACS on ice for 10 minutes followed by staining with 4G2-APC in 1x Flow Cytometry Perm Buffer on ice for 20 minutes ...
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bioRxiv - Bioengineering 2022Quote: ... and a cocktail of biotinylated detection antibody (~1 ng/ml per each protein panel) and phycoerythrin (PE, 532 nm emission) conjugated anti-human IgG antibody (10 ng/ml, Abcam) was loaded onto the barcoded array slide after removing excess serum using BSA solution ...
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bioRxiv - Biochemistry 2022Quote: ... Anti-G3BP1 beads were generated by incubating 200 μL Protein-G beads with 20 μL anti-G3BP1 antibody (Abcam, ab290) in 1 mL Buffer B (200 mM NaCl ...
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bioRxiv - Molecular Biology 2023Quote: ... the proteins were separated by 12 % SDS-PAGE and immunoblotted with anti-GST or anti-His antibody (Abcam, Cambs, England).
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bioRxiv - Neuroscience 2022Quote: ... Sections were then incubated overnight at 4°C with the polyclonal rabbit anti- myelin basic protein (MBP) antibody (1:200, ab40390, Abcam) diluted in blocking buffer ...
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bioRxiv - Neuroscience 2023Quote: ... proteins were transferred to PVDF membranes and then immunoblotted with the following antibodies: cleaved Caspase-3 (1:500, Abcam, ab13847), WAVE1 (1:1000 ...
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bioRxiv - Plant Biology 2022Quote: ... The separated proteins were then transferred to a nitrocellulose membrane and blotted with an anti-RFP antibody (Abcam No. 34767) at a dilution of 1/2,000.
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bioRxiv - Immunology 2022Quote: ... Proteins were transferred onto Hybond nitrocellulose membranes (Amersham biosciences) and probed with antibodies specific for alpha smooth muscle actin (Abcam), BACE1 (Sigma) ...
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bioRxiv - Neuroscience 2024Quote: ... Antibody-bound beads were generated by incubating 100μl of Protein G magnetic beads (Cell signalling, Cat# 9906) with 10μg anti-V5 antibody (Abcam, Cat# ab15828) overnight at 4°C ...
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bioRxiv - Immunology 2024Quote: ... 1:1000), GSDMD (Cat# ab209845, 1:1000) antibodies and recombinant human USP5 protein (Active) (IsoT, Cat# ab269124) were from Abcam. The mouse anti-Myc antibody (Clone 9E10 ...
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bioRxiv - Pathology 2024Quote: ... Each pre-cleared chromatin sample was then incubated with 35μl of protein A/G agarose beads pre-bound with 10μl of ChIP-grade rabbit anti-ESR1 antibody (Abcam, #ab32063) at 4℃ overnight ...
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bioRxiv - Cancer Biology 2021Quote: ... Interleukin 2 (IL-2, Abcam, Cambridge, UK) was used at a final concentration of 1 ng/ml ...
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bioRxiv - Molecular Biology 2020Quote: ... BSA in PBS containing 0.1% Tween-20 and incubated overnight at 4°C with primary antibodies to TSG101 (1:1000; Abcam, Cambridge, UK, Cat. # ab30871), CD63 (1:500 ...
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bioRxiv - Immunology 2021Quote: ... in phosphate buffered saline (PBS) prior to overnight incubation at 4°C with blocking buffer containing primary antibodies raised against HIS48 (Abcam, Ab33760, 1:20), CD68 (Abcam ...
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bioRxiv - Biochemistry 2021Quote: ... then incubated in 5% milk in TBS-T containing 1:10000 horseradish peroxidase (HRP) conjugated goat anti-mouse secondary antibody (Abcam ab6789-1 MG) for 1 hour at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... the slides were blocked with 10 % goat serum containing 1 % BSA at room temperature for 30 minutes and then incubated with the primary antibody against EGFP (Abcam, ab184601, 1:100) overnight at 4 °C ...
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bioRxiv - Neuroscience 2023Quote: ... slices were washed 3 times in 1x PBS for 10 minutes then incubated in blocking solution containing AlexaFlour488 conjugated secondary antibodies (Abcam, ab150077; 1:1000) for 2-4 hours at room temperature ...
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bioRxiv - Bioengineering 2024Quote: ... Incubation with the primary antibody was carried out at 4°C overnight in a PBS (1x) solution containing anti-Ki67 (Abcam, MA, 1:100), BSA (1%) ...
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bioRxiv - Genomics 2023Quote: ... cells were immunostained in blocking buffer containing mouse anti-beta III Tubulin (Tub) conjugated Alexa Fluor 488 antibody (Abcam ab195879, dilution 1:1000) and DAPI (Sigma D9542 ...
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bioRxiv - Neuroscience 2022Quote: ... Sections were incubated at room temperature for ~3 hours in secondary antibody solution containing donkey anti-rabbit IgG H&L Texas Red (abcam, Cambridge, UK; ab7081) and donkey anti-goat IgG H&L Alexa Fluor 488 (abcam ...
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bioRxiv - Immunology 2023Quote: ... then incubated overnight with a mix of primary antibodies, containing rabbit anti-CFTR (Alomone, 16 μg/ml) and chicken anti-GFP (Abcam, 35 μg/ml) at 4C ...
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bioRxiv - Biochemistry 2023Quote: ... Membranes were incubated for 20–40 min at 25 °C with Bullet ImmunoReaction Buffer (Nacalai Tesque, 18439-85) containing the following antibodies at the indicated dilution rate: H2AK5ac (Abcam, ab45152, 1/3,000), H2B (Cell Signaling ...
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bioRxiv - Cell Biology 2024Quote: ... cells were washed 3 times for 10 min with 1 ml of PBS and then incubated with 500 µl of secondary antibody containing donkey anti-rat IgG Alexa Fluor 594 (ab150156, Abcam, diluted 1:300) for 2 h at room temperature ...
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bioRxiv - Immunology 2024Quote: ... or a 36-hour incubation at room temperature with staining buffer containing 2.5 µg/mL of an antibody against vimentin (ab195877, abcam; nuclear and extracellular staining). The spheroids were then washed 3 times with the washing buffer and incubated with PBS containing 20 µM DRAQ5 for 4 hours ...
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bioRxiv - Molecular Biology 2024Quote: ... Nuclei were incubated in wash buffer containing primary antibody (anti-CRIP2, Abclonal A9038, lot 0050480101; rabbit polyclonal IgG, Abcam ab37415, lot #GR3208186-1) for 1 h ...
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bioRxiv - Neuroscience 2024Quote: ... at room temperature for 1 h and then incubated under agitation at 4° C overnight in a blocking solution containing the following primary antibodies: Rat anti-BrdU/CldU (Abcam, 1:1000, ab6326), and Mouse anti-IdU monoclonal antibody (Abcam ...
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bioRxiv - Cancer Biology 2024Quote: ... membranes were blocked with 5% non-fat milk in Tris-buffered saline containing 0.1% Tween® 20 (TBST) and incubated overnight with the following primary antibodies: CD163 (Abcam, Cat. No. ab182422), TNFα (Thermo Fisher ...
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bioRxiv - Cell Biology 2024Quote: ... cells were washed 3 times for 10 min with 1 ml of PBS and then incubated with 500 µl of secondary antibody containing donkey anti-rat IgG Alexa Fluor 594 (ab150156, Abcam, diluted 1:300) for 2 h at room temperature ...
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bioRxiv - Cell Biology 2020Quote: ... Blocking buffer was aspirated and cells incubated for 1 h at RT with a 1:1,000 dilution of primary antibodies in 2% BSA blocking buffer against dsDNA (Abcam, Cambridge ...
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bioRxiv - Neuroscience 2022Quote: ... blocked with 5% BSA and 0.2% triton X-100 for 2 h and incubated with primary antibody (rabbit anti-pMLKL, 1:200 Abcam) overnight at 4 °C ...
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bioRxiv - Cell Biology 2022Quote: ... 10mg replicate 2) were incubated for 4 h at 4°C with 5 µg anti-GFP antibody (Abcam ab290) or mock-treated as a control ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were incubated for 2 hours with secondary antibody Alexa Fluor 488 donkey anti-rabbit (1:1000 abcam ab150073), and glial fibrillary acidic protein (GFAP ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were fixed after 2 hour with 3.5% PFA and proceeded with IF staining with anti-BrdU antibody (ab6326, Abcam) followed with the secondary antibody conjugated with Alexa Fluor 594.
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bioRxiv - Biochemistry 2021Quote: ... primary antibodies (OAT, Invitrogen #PA5-66715, 1:500; Fumarase, Invitrogen #PA5-82899, 1:500; Casein Kinase 2 beta, Abcam #ab151784 ...
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bioRxiv - Cell Biology 2021Quote: ... A monoclonal antibody that detects serine or threonine linked O-Linked N-Acetylglucosamine-RL-2 (ab2739) was from abcam. Anti-OGT (ab177941) ...
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bioRxiv - Neuroscience 2023Quote: ... 100□μl of supernatant was saved as “Input.” The remaining supernatant was transferred to a 2-ml round-bottom tube and incubated with 5Lμg/μl of anti-GFP antibody (ab290; Abcam) at 4°C with end-over-end rotation for 1 h ...
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bioRxiv - Cancer Biology 2024Quote: ... blocked with 2% BSA and incubated with primary rabbit anti-XBP1 antibody (ab37152, 1:200, Abcam, Cambridge, MA, USA), ATF6 antibody (ab203119 ...
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bioRxiv - Neuroscience 2024Quote: ... sections were incubated in either recombinant primary rabbit anti-GFAP antibody (astrocytes, 1:500 in 2% BSA and 0.1% Triton [TBS]; Abcam), or recombinant primary rabbit anti-ionised calcium binding adaptor molecule 1 (Iba1 ...
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bioRxiv - Neuroscience 2024Quote: ... or recombinant primary rabbit anti-ionised calcium binding adaptor molecule 1 (Iba1) antibody (microglia, 1:1000 in 2% BSA and 0.1% Triton [TBS]; Abcam) overnight ...