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Citations for abcam :
851 - 900 of 10000+ citations for 5 1 3 benzothiazol 2 ylamino pentanoic acid since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2020Quote: ... rabbit-anti-caspase-3 (Abcam, ab13847) at 1:300 dilution ...
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bioRxiv - Genetics 2022Quote: ... 3 μg of H3K9me2 antibody (Abcam) was added to the remaining ∼900 μL of lysate and the remaining IP steps were carried out as described previously using 20 μL Dynabeads protein G (Invitrogen by ThermoFisher ...
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bioRxiv - Cell Biology 2023Quote: ... CK666 (Arp2/3 inhibitor, Abcam ab141231), SMIFH2 (Formin inhibitor ...
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bioRxiv - Microbiology 2023Quote: ... and anti-Histone 3 (Abcam, ab1791) antibodies.
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bioRxiv - Cancer Biology 2024Quote: ... Netrin-3 (Abcam rabbit antibody #ab185200), α-tubulin (Cell Signaling Technology anti-αTubulin rabbit antibody #2125 ...
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bioRxiv - Bioengineering 2024Quote: ... rabbit anti-Caspase 3 (ab32351, Abcam), goat anti-PDX1 (AF2419 ...
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bioRxiv - Developmental Biology 2021Quote: ... The mouse anti-DP Yun (#6, 1:5 (Du et al., 1996) and the mouse beta actin (1:1000, Abcam, Cat#ab8224) antibody was used as loading control in western blot assays.
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bioRxiv - Neuroscience 2020Quote: ... The fixed cells were blocked with 5% normal goat serum for 4 hours followed by incubation with anti-Iba-1 antibody (1:300, Abcam, Cambridge, UK) for 12 hours at 4°C to label microglial cells ...
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bioRxiv - Systems Biology 2022Quote: ... Cells were tehen incubated with a primary antibody solution containing BrdU antibody diluted 1:400 or 1:1000 in PBS containing 5% goat serum (Abcam, catalog # ab7481) and 0.1% Triton-X for 1 hour at room temperature ...
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bioRxiv - Bioengineering 2023Quote: ... which was prepared by 5% milk in TBS-T containing 1:10,000 horseradish peroxidase (HRP)-conjugated goat anti-mouse secondary antibodies (Abcam ab6789-1 MG). The secondary antibody incubation was applied for 1 hour at room temperature ...
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bioRxiv - Biochemistry 2023Quote: ... Stripped membranes were blocked in 5% NFDM in TBST (1 h, RT) before being probed with anti-Act1 (Abcam ab8224, 1:5000) loading control antibody (1 h ...
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bioRxiv - Cancer Biology 2023Quote: ... The membrane was blocked with 5% skim milk for 1 hour at RT before immunoblotting with antibodies against ACC1 (#ab72046, Abcam, 1:2000), ACC2 (#HPA006554 ...
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bioRxiv - Cell Biology 2024Quote: ... for 1h followed by antibody incubation in 5% Bovine Serum Albumin (BSA) in TBS-T overnight (TfR, 13-6800, ThermoFisher, 1:250; ApoE, Ab947, abcam, 1:500). After three times washing in TBS-T ...
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bioRxiv - Synthetic Biology 2020Quote: ... 5 mM CaCl2) and 1μl of 1 mg/ml aprotinin (activation buffer; #Ab146286, Abcam MA, USA). These reactions were incubated for 4 h at 37°C with 500 rpm shaking (Eppendorf ...
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bioRxiv - Cell Biology 2020Quote: ... diluted in 5% milk prepared in IX TBS – 0.1% Tween20: anti-RPL26 (Abcam #ab59567; 1:2000), anti-RPS6 (Abeam #ab40820 ...
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bioRxiv - Pathology 2020Quote: ... Primary antibodies used included rabbit anti-Laminin alfa 5 diluted at 1:100 (Abcam; Cambridge, UK), anti-rabbit cytokeratin K14 diluted to 1:250 (Proteintech ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... IHC staining for human leukocyte antigen (HLA) primary antibody (Abcam, clone EMR8-5, 1:50 dilution) and N-sulfated heparan sulfate (Amsbio ...
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bioRxiv - Cell Biology 2022Quote: ... and incubated with primary antibodies in 5% BSA TBS-T against SDHA (1:10000, Abcam; ab14715), MT-CO1 (1:10000 ...
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bioRxiv - Neuroscience 2022Quote: ... blots were incubated overnight with primary antibodies: anti-beta actin (1:2500 in 5% BSA, Abcam), anti-LC3B (1:1000 in 5% BSA ...
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bioRxiv - Microbiology 2024Quote: ... and histone H3 were detected with anti-HA (Abcam 9110, 1:1,000 in 5% milk-PBST) and anti-H3 (Abcam ab1791 ...
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bioRxiv - Immunology 2023Quote: ... sections were incubated for 1 hour at RT with D-PBS (-) + 5 % normal goat serum (Abcam) to block nonspecific binding sites and then incubated overnight at 4 °C with primary antibodies diluted in D-PBS (-) ...
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bioRxiv - Cancer Biology 2023Quote: ... The antibodies were incubated with primary antibody anti-CXCR4 (1:1000 in 5% BSA) (Abcam, ab181020) or β-actin (1:10000 in 5% milk in TBST ...
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CRISPR-SID: identifying EZH2 as a druggable target for desmoid tumors via in vivo dependency mappingbioRxiv - Cancer Biology 2021Quote: ... Slides were subsequently blocked with blocking buffer (3% natural serum, 1% BSA, 0.1% Tween-20) and incubated overnight with anti-EZH2 antibody (3748; Abcam), anti-CREB3L1 (AF4080 ...
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bioRxiv - Neuroscience 2021Quote: ... plus 5% normal goat serum for 1 hr at RT before 3 d incubation in primary antibody diluted in block (1:1,000, chicken anti-GFP, Abcam, ab13970 ...
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bioRxiv - Cell Biology 2022Quote: ... cells were incubated overnight at 4 °C in a mixture of rabbit anti-Cav-3 (1:100, ab2912, Abcam) antibody and a custom rabbit anti-NCX1 antibody as previously described5 (1:100 ...
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bioRxiv - Microbiology 2020Quote: ... washed 3 times with TBS-T and primary 4-HNE antibody was added at 1:200 dilution (Abcam, #ab46545). The antibody was left on overnight at 4°C rocking ...
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bioRxiv - Neuroscience 2022Quote: ... and incubated overnight in 3% normal donkey serum and 0.3% PBST containing primary antibodies for GFP (1:2000, chicken anti-GFP, ab13970, Abcam), and/or TH (1:2000 ...
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bioRxiv - Biophysics 2024Quote: ... Attached cells were then fixed by methanol/acetic acid solution (3:1) stained with Perl’s Prussian blue with nuclear fast red as counterstain (Iron stain kit, ab150674, abcam). Slides were dehydrated (incubation with 75 ...
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bioRxiv - Microbiology 2022Quote: ... then incubated for 1 h at room temperature in 20 mL TBS-T with 3% milk powder and 1:5,000 diluted anti-GFP antiserum (Abcam) while shaking with 180 rpm ...
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bioRxiv - Biochemistry 2023Quote: ... The membrane was incubated overnight at 4°C with 1:500 diluted anti-14-3-3β antibody (abcam ab15260) in 5% BSA/TBST ...
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bioRxiv - Neuroscience 2023Quote: ... the sectioned slices were incubated for one hour at room temperature in blocking solution (10% normal goat serum and 0.3% Titron X-100 in 1x TBS) and incubated overnight in polyclonal rabbit anti-HA (1:1,000, #ab9110, Abcam) at 4°C ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were incubated overnight in primary antibodies then washed (3 x 0.1M PBS) and incubated in secondary antibodies conjugated to Alexa-594 (1:200, Abcam) or Alexa-488 (1:200 ...
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bioRxiv - Neuroscience 2024Quote: ... Brain floating slices were incubated 3 days at 4°C in the blocking condition with primary antibody chicken anti-EGFP (1:1,000; ab13970, Abcam). After three PBS washes ...
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bioRxiv - Genomics 2022Quote: ... A cocktail of antibodies against the glucocorticoid receptor was added to chromatin input for immunoprecipitation: 2 μg of ab3570 (Abcam, lot GR3222141-5, discontinued) and 0.49 μg of 12041S (Cell Signaling Technology ...
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bioRxiv - Microbiology 2020Quote: ... The cells were then probed for 1 h with a polyclonal rabbit anti-SARS-CoV-2-S protein antibody (1:2,250; ab252690; Abcam, Cambridge, UK) or a rabbit anti-MeV N protein antibody (1:1,000 ...
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bioRxiv - Microbiology 2022Quote: ... low or no ACE2 were resuspended in FACS buffer (PBS+2% BSA) and incubated for 1 h with 1:500 rabbit anti-ACE2 antibody (Abcam ab272500). Cells were washed with FACS buffer and labeled for 1 h with 1:3000 Alexa-Fluor-488 goat anti-rabbit IgG H&L (Abcam ab150077) ...
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bioRxiv - Cell Biology 2021Quote: ... and reacted with an antibody to synaptic vesicle 2 (SV2 1:50; Developmental Studies Hybridoma Bank Ab2315387) and neurofilament 200 (NF200 1:500, Abcam Ab72996) for 24 hours at 4°C ...
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bioRxiv - Bioengineering 2021Quote: ... diluted at 1:100 followed by 2 h of incubation with 1:500 AlexaFluor 568 goat anti-mouse IgG (abcam, UK) diluted at 1:500 ...
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bioRxiv - Biochemistry 2021Quote: ... Standard Western Blot analysis using α-B3GLCT (IHC-plus antihuman B3GALTL antibody, 1:500) and α-rabbit-HRP (2°, 1:5,000, Abcam)] was performed 9 ...
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bioRxiv - Genetics 2021Quote: ... Cells were incubated with primary antibody (1:400 RNAPII [pSer2] Novus Biologicals NB100-1805, 1:400 PCNA [5E6/2] abcam ab70472) overnight at 4°C ...
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bioRxiv - Immunology 2023Quote: ... or phenol red-free RPMI-1640 were serially diluted (2-fold) and mixed 1:1 with 0.2 mM nitrocefin (Abcam, Cambridge, UK). Samples were incubated 1–16 h at room temperature and absorbance at 490 nm was read on a SpectraMax M2 plate reader (Molecular Devices ...
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bioRxiv - Genetics 2023Quote: ... followed by a permeabilisation step using 0.3% Triton X-100 for 10 min and then treated with 1% BSA blocking buffer solution for 2 h prior to overnight incubation with monoclonal anti-pNRF2 (1:200; Abcam ab76026) in a humidified chamber at 4°C ...
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bioRxiv - Developmental Biology 2023Quote: Western blot analysis was done as described (IHARA AND NISHIWAKI 2007) using anti-NID-1 (2 μg/ml) (KUBOTA et al. 2008) and anti-α-tubulin (DM1A, 1:1000; abcam), anti-GFP (3E6 ...
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bioRxiv - Cell Biology 2023Quote: ... Blocked cells were then incubated for 1 hour at room temperature with primary antibodies anti-Isl1/2 (Abcam ab109517, 1:2000), anti-Chx10 (Santa Cruz Biotechnology sc-365519 ...
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bioRxiv - Bioengineering 2023Quote: ... Blocking was conducted using 2% w/v BSA in 1×PBS for 1 h and incubated with anti-Ki67 antibody (Abcam ab16667) in a 1:200 dilution ...
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bioRxiv - Developmental Biology 2024Quote: ... blocked with 5% normal goat serum/2% BSA/0.1% Triton-X for 1 hour and incubated with mouse monoclonal PAX7 antibody (abcam, 1:200) and rabbit monoclonal SOX10 antibody (Cell Signaling Technologies ...
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bioRxiv - Cell Biology 2024Quote: ... fixed samples were incubated with 400 µL of a 1 µg mL-1 Alexa Fluor 647 Rabbit Monoclonal anti-FGF-2 IgG (abcam, USA) solution in PBS with 10 % Goat Serum (v/v ...
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bioRxiv - Neuroscience 2022Quote: ... Primary antibodies included rat monoclonal anti-VCAM1 (Abcam, ab19569, clone M/K-2; 1:250) and Armenian hamster anti-PECAM-1 (CD31 ...
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bioRxiv - Cancer Biology 2020Quote: ... and 1:2000 dilution of Anti-Histone H2A.X (phosphor S139) [EP854(2)Y] (Abcam, ab81299) for an hour at 37°C ...
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bioRxiv - Microbiology 2022Quote: ... mouse anti SARS-CoV-1 and 2 Spike protein clone [1A9] monoclonal (Abcam, cat# ab273433) (1:200 dilution ...