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Citations for abcam :
8751 - 8800 of 10000+ citations for 7 BROMO 2 1 3 BENZOTHIADIAZOLE 4 SULFINIC ACID since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2024Quote: ... anti-Iba1 (Abcam; #ab178847; 1:1,000), and anti-NeuN ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were fixed in 2% formaldehyde in PBS for 10 min at room temperature and stained with Phalloidin-iFluor 594 (abcam, ab176757) for 30 minutes at room temperature ...
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bioRxiv - Cell Biology 2020Quote: ... the chromatin fraction was solubilized in EBC-150 buffer with 500 U/mL Benzonase and 2 ug of antibody directed to endogenous RNAPII-S2 (Abcam; ab5095) or RNAPII-S5 (Abcam ...
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bioRxiv - Cell Biology 2020Quote: ... Unbound primary antibody was removed the following day by washing in 0.2% Tween 20 in PBS and blastocysts incubated for 2 hr at RT with goat anti-rabbit Alexa-488 conjugated secondary antibodies (Abcam, ab150077). Following washing ...
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bioRxiv - Developmental Biology 2021Quote: ... slides were incubated with secondary antibody for 2 h at room temperature before DAB reaction using the DAB detection kit (Abcam #ab64238). Slides were counterstained with hematoxylin to stain nuclei ...
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bioRxiv - Neuroscience 2021Quote: ... end stage high- titer viral aliquots or 2) from cell culture media used for rabies virus rescue after PEG- based precipitation (Abcam, ab102538) and quantified using the High Sensitivity RNA ScreenTape assay (Agilent ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were again washed thrice in 0.1 M PB and then incubated with the secondary antibody for 2 h (goat anti-chicken-IgY conjugated to Alexa Fluor 594, Abcam, ab150172). Following the incubation with the secondary antibody ...
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bioRxiv - Immunology 2019Quote: ... samples were incubated in succession in secondary antibody solution for 2 hr at RT (donkey anti-rabbit IgH&L Alexa594, Abcam, ab150076; donkey anti-goat IgH&L Alexa488, Abcam, ab150129). Tissues were washed in 1% Triton X-100/PBS ...
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bioRxiv - Neuroscience 2020Quote: ... sections were washed 3 times with PB and then incubated with the secondary antibody for 2 hours (goat anti chicken Alexa Fluor 594, abcam, ab150172). After incubation with the secondary antibody ...
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bioRxiv - Neuroscience 2020Quote: ... followed by a 2 hr incubation with a mixture of the secondary antibody (goat anti-mouse Alexa Fluor® 488–conjugated IgG, ab150113 Abcam) and Alexa Fluor 568 conjugated Phalloidin (A12380 ...
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bioRxiv - Cell Biology 2021Quote: ... for 2 hours at room temperature (Chicken anti-GFP, Aves cat#GFP-1020, RRID:AB_10000240; Rabbit anti-NUP153, Abcam cat#ab84872, RRID:AB_1859766), followed by washing 3x 5 min in 1x PBS ...
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bioRxiv - Microbiology 2021Quote: ... Platelets were then permeabilized with 0.25% saponin for 30 min and stained using recombinant anti-Bcl-2 antibodies (AF647, Abcam, Berlin, Germany) for 30 min before being measured by flow cytometry ...
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bioRxiv - Microbiology 2021Quote: ... Gels were either stained with Coomassie brilliant blue or subjected to Western blot using appropriate antibodies: rabbit polyclonal anti-SARS-CoV-2 S antibody (Abcam ab272504) or rabbit polyclonal anti-SARS-CoV/CoV2 M antibody (Novus Biologicals NB100-56569).
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bioRxiv - Bioengineering 2022Quote: ... Samples were washed in distilled water and stained for 15 min in 2% w/v Alizarin Red (ab146374, Abcam, Cambridge, UK) in distilled water at a pH of ~4.2 ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... The sections were rinsed with PBS and blocked for 2 hours at room temperature using 10% normal donkey serum (NDS; Abcam, #ab7475) prepared in 1% BSA in PBS ...
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bioRxiv - Molecular Biology 2019Quote: ... Chromatin immunoprecipitation was performed by incubation of the chromatin fraction overnight with 20 μl of protein-A coated beads (Thermo-Scientific, 53139) and 2 μg of H3K4me1 specific (Abcam, ab8895) or 2 μg of H3K4me3 specific (Abcam ...
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bioRxiv - Neuroscience 2022Quote: ... Floating sections were captured onto gelatin slides in serial order from caudal to rostral brainstem and mounted with 4’6’-diamidino-2-phenylindole dihydrochloride (DAPI) (Abcam, Cambridge, MA).
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bioRxiv - Developmental Biology 2024Quote: ... Embryos were blocked at room temperature for 2 hours in TBTD supplemented with 10% donkey serum and incubated in anti- SOX2 (AbCam, #ab97959) and anti-SOX17 (R&D ...
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bioRxiv - Cell Biology 2024Quote: ... The cell extract was then incubated with magnetic beads conjugated with anti-Argonaute 2 (AGO2) or anti-IgG antibody (Abcam, USA) overnight at 4 °C ...
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bioRxiv - Cell Biology 2023Quote: ... U-2 OS transiently overexpressing mCherry-Sec61β were prepared for immunofluorescence as described above using a rabbit anti-Rtn4 primary (Abcam; ab47085) with a goat anti-rabbit secondary conjugated to Alexa Fluor 647 (Life Technologies ...
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bioRxiv - Neuroscience 2023Quote: ... The sections were incubated for 2 h at 25°C with appropriate secondary florescence labelled antibodies (donkey anti-goat IgG, Alexa fluor 488 (Abcam, ab150129) & donkey anti-rabbit IgG ...
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bioRxiv - Microbiology 2023Quote: ... 5 s at 60% amplitude) in 320 µL of buffer A containing 2 % (v/v) NP-40 (Abcam, Cat. No. ab142227). The aggregated proteins were sedimented at 15,000 x g for 30 min at 4 °C ...
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bioRxiv - Cell Biology 2023Quote: ... Grids were washed in blocking buffer 5x 2 mins and then incubated in 10 nm colloidal gold-conjugated anti-rabbit secondary antibody (Abcam, ab27234) diluted 1:20 in blocking buffer for 30 min ...
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bioRxiv - Physiology 2023Quote: ... Immunoprecipitation of the cleared lysates was performed overnight at 4C with 5μg of anti-VDR antibody or anti-RXRα monoclonal antibodies (Abcam – Table 2). The beaded complexes were washed several times with NP-40 lysis buffer ...
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bioRxiv - Microbiology 2023Quote: ... Specific sIgA antibodies to the SARS-CoV-2 virus were detected with goat anti-mouse IgA alpha-chain + HRP (Abcam®), diluted to 1:1.000 ...
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bioRxiv - Microbiology 2023Quote: ... the cell lysate was split into two equal volumes and combined with 2 μg of rabbit CCT2 specific monoclonal antibody (Abcam, ab92746), 2 µg of rabbit anti-CCT3 (Ray Biotech ...
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bioRxiv - Genomics 2023Quote: ... CUT&Tag was performed as previously described93 (detailed protocol DOI: dx.doi.org/10.17504/protocols.io.z6hf9b6) using (Abcam catalog no. ab9050, lot GR3386101-2) polyclonal antibody ...
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bioRxiv - Microbiology 2023Quote: ... and incubated with the SARS-CoV-2 Spike Antibody Mouse Mab (40592-MM117, SinoBiological, China) and HRP-conjugated goat anti-mouse IgG (ab6789, abcam, UK). Detection was carried out using an Ultra High Sensitivity ECL Kit (HY-K1005 ...
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bioRxiv - Cell Biology 2023Quote: ... and the following secondary antibodies in blocking solution for 2 h at RT: Alexa Fluor 488® goat anti-guinea pig IgG H&L (Cat. No. ab150185; Abcam, UK ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... The membrane was then incubated in blocking buffer (5% skim milk in TBS/0.05% tween-20) followed by blotting with primary antibodies (anti-SARS-CoV-2 spike (Abcam ab275759, USA) 1:2,000 or anti-β-actin (Sigma A2228 ...
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bioRxiv - Microbiology 2024Quote: ... the cell lysate was split into equal volumes and combined with 2 µg mouse mAb anti-V5 (SV5-PK1) (Abcam, ab27671), mouse mAb anti-HA (clone HA-7)(Merck ...
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bioRxiv - Bioengineering 2021Quote: ... R&D Systems, 4 μg/mL; vimentin, ab92547, Abcam, 0.5 μg/mL; CD31, AF3628, R&D Systems, 10 μg/mL; desmin, ab227651, Abcam, 1.32 μg/mL) were included in the AZP solution ...
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bioRxiv - Cancer Biology 2024Quote: ... Chromatin extract was sonicated with Bioruptor (20 min; pulse: 30 s on/30 s off; high amplitude) and immunoprecipitated with H3K27ac antibody (Abcam, ab4729, rabbit polyclonal, 4 µg/ChIP) premixed with Dynabeads A and G ...
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bioRxiv - Animal Behavior and Cognition 2023Quote: ... and then incubated for approximately 20 hours at 4°C in either rabbit anti-α-synuclein (α-syn) phosphorylated serine 129 (P-S129; Abcam #EP1536Y, Cambridge, United Kingdom) diluted to 1:1000 in blocking solution or rabbit anti-tyrosine hydroxylase (TH ...
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bioRxiv - Cell Biology 2022Quote: ... and beta-catenin (#9562, 1:200, Cell Signalling Technologies) or gamma-tubulin (GTU-88, 1:400, Abcam) overnight at 4°C ...
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bioRxiv - Molecular Biology 2020Quote: ... Horseradish peroxidase-conjugated goat anti-mouse (1:5000) or anti-rabbit (1:10000) secondary antibodies (Abcam, MA) were used at room temperature for 1 hour ...
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bioRxiv - Cancer Biology 2020Quote: ... for 1 hour and then incubated overnight at 4C°with anti-rabbit CD31 antibody (1:100, Abcam) or 1 hour at room temperature for anti-rabbit PAX2 (1:1000 ...
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bioRxiv - Immunology 2021Quote: ... sheep anti-VWF (ab11713, 1:200-400) and rabbit anti-TPC1 (ab94731, 1:200) were from Abcam, rabbit anti-TRPML1 was from Thermo Fisher Scientific (PA1-464741 ...
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bioRxiv - Cell Biology 2021Quote: ... cardiomyocytes were incubated with primary antibodies (anti-dystrophin 1:200, Abcam; anti-4HNE 1:200, Alpha Diagnostic) overnight at 4°C ...
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bioRxiv - Bioengineering 2021Quote: ... 1:1000 rabbit polyclonal anti-PGAM5 antibody (Novus) or 1:1000 rabbit polyclonal anti-γH2AX antibody (Abcam) for 90 min at room temperature ...
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bioRxiv - Neuroscience 2022Quote: ... The concentrations of primary antibodies were rabbit anti-pS129 αSyn (1:10,000, Abcam Cat# 1536-1, RRID:AB_562180), mouse anti-αSyn (1:1,000 ...
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bioRxiv - Microbiology 2022Quote: ... Cells were stained with rabbit-anti-Lamp1 primary antibody (1:1000, 1 hr, 37°C, Abcam #ab24170), washed 3x with PBS ...
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bioRxiv - Neuroscience 2022Quote: ... Blots processed with blocking solution were incubated with antibodies against VCAM-1 (ab134047, Abcam, 1:1000 dilution), E-Selectin (AF724 ...
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bioRxiv - Immunology 2022Quote: ... conjugated primary antibodies cocktail (Akoya CD3e 1:100 and Akoya CD20 1:200 in Abcam Antibody Diluent) 2x for 90 minutes each ...
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bioRxiv - Neuroscience 2020Quote: ... Cells were then incubated with primary antibody (anti-Musashi-1: 1/300, polyclonal rabbit: Abcam ab21628, UK) in blocking solution for 48 h at 4 °C ...
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bioRxiv - Neuroscience 2021Quote: ... Primary antibodies were then diluted in BB blocking buffer (81A 1:500; D2G40 1:500; MAP2 Abcam #ab5392 ...
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bioRxiv - Cancer Biology 2019Quote: ... The slides were incubated with anti-CD8 (1:500) and anti-CD155 (1:100) (Abcam, Hong Kong) primary antibodies at 4°C overnight ...
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bioRxiv - Molecular Biology 2019Quote: ... PfAldolase was detected using an HRP conjugated anti-PfAldolase (Abcam # ab38905, 1:5000 in 1% milk PBST) antibody ...
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bioRxiv - Developmental Biology 2020Quote: ... chicken polyclonal anti-GFP (ab13970, 1:1000), mouse monoclonal anti-c-Myc (ab32, 1:200) from Abcam. For immunoblotting analysis ...
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bioRxiv - Neuroscience 2020Quote: ... anti-mouse Iba1 (019-19741, Wako Chemicals, 1:500) and anti-mouse CD45 (ab23910, Abcam, 1:500); panel 3 = anti-mouse BrdU (ab8152 ...