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Citations for abcam :
8501 - 8550 of 10000+ citations for Anti INTS9 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... 1:400 dilution) and anti-α- synuclein aggregate antibody (Clone#: MJFR-14-6-4-2; 1:1000 dilution) were acquired from Abcam (Cambridge UK).
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bioRxiv - Molecular Biology 2022Quote: ... The membranes were blocked with 5% skimmed milk for 2 h at room temperature and then incubated with primary antibodies overnight at 4 °C as follows: anti-collagen I (ab270993; Abcam, USA; 1:1000), anti-α-smooth muscle actin (α-SMA ...
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bioRxiv - Immunology 2023Quote: ... Cells were incubated at room temperature (RT) for 30 min protected from light in the presence of anti-spike antibody (Abcam, clone 1A9, ab273433) labeled with Alexa Fluor®-647 (Alexa Fluor® 647 Conjugation Kit (Fast)-Lightning-Link® (Abcam ...
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bioRxiv - Cell Biology 2022Quote: ... Blots were then blocked using 5% non-fat dry milk (NFDM) for 45 minutes at room temperature and incubated with primary antibodies: anti-GFP (catalog number ab290; Abcam; dilution 1:5000) and anti-GAPDH (catalog number MA5-15738 ...
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bioRxiv - Molecular Biology 2023Quote: ... DNA detection was performed by applying 2 ng of the sample used for PicoGreen detection to a nylon membrane and detecting with the anti-dsDNA antibody (Abcam #ab27156, 1:9000).
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bioRxiv - Molecular Biology 2023Quote: ... Blocking buffer was discarded and slides were washed and incubated with primary antibody: rabbit anti-Alpha-synuclein (phospho S129) (Abcam #ab51253, 1:10000) diluted in blocking buffer overnight ...
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bioRxiv - Cell Biology 2023Quote: ... or anti-Sc35 (MilliporeSigma, S4045, 1:1000) and incubated with the secondary antibodies using Alexa 488-labeled goat anti-mouse IgG (Abcam, Ab150113, 1:1000), Alexa 594-labeled goat anti-rabbit IgG (ThermoFisher ...
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bioRxiv - Immunology 2023Quote: ... 7 μm thick frozen sections of ileal tissue were blocked in 10% NGS and stained with biotinylated anti-GFP antibody (Abcam, ab6658, 1:250) or anti-occludin AlexaFluor 594 (Invitrogen ...
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bioRxiv - Microbiology 2023Quote: ... Primary IgG antibodies specific to Lamin B1 (#ab16048) and the secondary IgG donkey anti-rabbit AF405 conjugated antibody (#ab175651) were purchased from Abcam (San Francisco, A). The SNAP-Cell® TMR-Star and Oregon Green® were purchased from New England Biolabs (NEB ...
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bioRxiv - Neuroscience 2023Quote: ... sections were mounted on slides and stained using a monoclonal mouse anti-muscarinic acetylcholine receptor M4/CHRM4 primary antibody (1:500, abcam, ab77956, RRID: AB_1566454), with blocking reagents and secondary anti-mouse biotinylated antibody from the M.O.M ...
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bioRxiv - Bioengineering 2022Quote: ... Each well was blocked with 5% BSA-tween solution and incubated with a primary mouse anti-human α-SMA antibody (ab7817, Abcam, Cambridge, UK) solution at a 1:100 dilution overnight at 4°C ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were then permeabilized and blocked with the blocking buffer [0.1% Triton X-100 and 2% bovine serum albumin in phosphate buffered saline (PBS)] for 30 min and subsequently incubated with the primary antibodies rabbit anti-αTubulin (Abcam, ab18251,1:1000 dilution), mouse anti-acetylated Tubulin (Sigma ...
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bioRxiv - Cell Biology 2023Quote: ... After washing the membranes in TBST (3 × 5 min), they were probed (1.5 h, RT) with the HRP-conjugated secondary antibodies: goat anti-mouse IgG (Abcam Cat# ab205719, 1:10000) or goat anti-rabbit IgG (Abcam Cat# ab205718 ...
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bioRxiv - Immunology 2023Quote: ... and blocked in 2% heat-inactivated-FBS for 1 hour at room temperature followed by incubation with primary antibodies polyclonal rabbit anti-H3Cit (Abcam, ab5103, 1μg/mL) and goat anti-Myeloperoxidase (R&D systems ...
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bioRxiv - Immunology 2023Quote: ... Cells were incubated at room temperature (RT) for 30 min protected from light in the presence of anti-spike antibody (Abcam, clone 1A9, ab273433) or anti-nucleocapsid (Abcam ...
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bioRxiv - Biochemistry 2023Quote: ... After washing with PBS a 2.5% BSA solution was applied for 1 hour and then incubated with primary anti-human XOR rabbit antibody (1:2000, Abcam 133268 RRID: AB_11154903) and left over-night at 4 °C ...
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bioRxiv - Neuroscience 2022Quote: ... the sections were washed three times in PBST and incubated with secondary antibodies diluted in blocking solution for two hours at room temperature (RT): Goat anti-Rabbit conjugated Alexa Fluor 568 (Abcam #175471, 1:500) and 1:500 Goat anti-Chicken Alexa Fluor 488 (A11039 ...
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bioRxiv - Immunology 2023Quote: ... Membranes were washed with TBS-T and then incubated with HRP-conjugated secondary antibodies for 1 h at RT: goat anti-rat IgG at 1:2000 (Abcam, Waltham, MA #ab97057), sheep anti-mouse IgG at 1:2000 (GE Cytiva #NA931) ...
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bioRxiv - Immunology 2023Quote: ... Cells were incubated at room temperature (RT) for 30 min protected from light in the presence of anti-spike antibody (Abcam, clone 1A9, ab273433) or anti-H3 (A/Perth/16/2009 ...
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bioRxiv - Systems Biology 2023Quote: ... the cells underwent an additional 30-minute blocking step using 1× blocking buffer and were then incubated for 1 hour with secondary antibodies – Goat Anti-Mouse IgG H&L (Alexa Fluor® 594, Abcam, Cambridge, UK), and Phalloidin-iFluor 488 Reagent (Abcam ...
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bioRxiv - Microbiology 2023Quote: FFPE sections were evaluated by multiplex immunohistochemical analysis following previously reported protocol using antibodies specific for anti-α-SMA (ab124964, Abcam, 1:500), anti-IL-8/IL-8 (MAB208 ...
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bioRxiv - Biochemistry 2023Quote: ... for 10 min and blocked in 0.5% BSA in 0.5% Tx-PBS for 1 h at room temperature before incubation with mouse monoclonal anti-ψ-tubulin antibody (Abcam, ab11316, 1:1000) in 0.5% BSA in 0.5% Tx-PBS overnight at 4 ºC ...
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bioRxiv - Neuroscience 2023Quote: ... The slides with cryosectioned tissue were washed with PBS followed by incubation in blocking solution (2% of BSA in PBS) for 1 h and then incubated overnight at 4°C with an anti-DBI antibody (rabbit, 1:1000, Abcam, Cat. No: ab231910). Sections were then incubated for 1 h at room temperature with anti-rabbit Alex Fluor 546 (1:1000 ...
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bioRxiv - Neuroscience 2023Quote: ... DRG or spinal sections were incubated with secondary antibodies for 2 h: goat anti-mouse IgG H&L (Alexa Fluor® 488) (1:500, Abcam, Cat#: ab150113), goat anti-guinea pig IgG H&L (Alexa Fluor® 488 ...
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bioRxiv - Developmental Biology 2023Quote: ... was used as a positive control to confirm a successful experiment and a secondary antibody anti-Mouse IgG (Abcam, ab46540, 1:50 dilution) was used as negative control to remove background in the following sequencing analysis.
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bioRxiv - Developmental Biology 2023Quote: ... Fractions pellet was resuspended in SDS sample buffer and analyzed by Western blotting (WB) using anti-GFP antibody (Abcam, ab 290, 1:1,000). GFP positive fractions (5 and 6 ...
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bioRxiv - Bioengineering 2023Quote: ... 6X-His Tag Monoclonal Antibody (HIS.H8) and Goat Anti-Mouse IgGH&L (HRP) were preferred (Thermo Scientific, Cat.#MA1-21315, Abcam, Cat.#ab6789, respectively). For HisTag purification ...
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Linear ubiquitination at damaged lysosomes induces local NF-κB activation and controls cell survivalbioRxiv - Cell Biology 2023Quote: ... Secondary antibody incubation was done for 1 h at RT with the following horseradish peroxidase (HRP)-coupled antibodies: HRP-conjugated goat anti-mouse IgG (Abcam Cat# ab6789, RRID:AB_955439), HRP-conjugated goat anti-rabbit IgG (Abcam Cat# ab6721 ...
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bioRxiv - Cell Biology 2024Quote: The following antibodies were used for western blotting at the indicated dilutions: anti-CD63 (MBL, D263-3, 1:5000; Abcam, ab134045, 1:2000), anti–Flotilin-1 (BD Biosciences ...
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bioRxiv - Cell Biology 2024Quote: ... cells were washed 3 times for 10 min with 1 ml of PBS and then incubated with 500 µl of secondary antibody containing donkey anti-rat IgG Alexa Fluor 594 (ab150156, Abcam, diluted 1:300) for 2 h at room temperature ...
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bioRxiv - Cancer Biology 2024Quote: ... After blocking with 5% skim milk (Beyotime, China) and sequential incubation with the primary and secondary antibodies: anti-PRMT3 (Abcam, Ab191562, 1:2000), anti-PRMT3 (Proteintech ...
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bioRxiv - Molecular Biology 2024Quote: ... midguts were incubated at room temperature for 2 hours on an orbital shaker with secondary antibodies (goat anti-rabbit IgG Alexa Fluor 488 [Abcam] at 1:4000) diluted in midgut blocking buffer ...
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bioRxiv - Plant Biology 2024Quote: ... incubated with one of the following secondary antibodies as appropriate: Horseradish peroxidase (HRP)-conjugated goat anti-rabbit (Abcam, catalog no. ab97051; 1:10,000) or HRP-conjugated goat anti-mouse (Abcam ...
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bioRxiv - Bioengineering 2024Quote: ... 3D cultured cells were washed with the washing solution and then incubated with goat anti-rabbit secondary antibody Alexa Fluor 488 (ab150077; Abcam, Toronto, ON, Canada) for 2 hours at room temperature ...
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bioRxiv - Pathology 2024Quote: ... IHC protocol modified from a previously described protocol(37, 38) using a rabbit anti-SARS-CoV-2 S protein antibody (Abcam, ab272504, Cambridge, UK) at 1:5000 dilution (0.2 µg/mL) ...
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bioRxiv - Bioengineering 2024Quote: ... Then the membrane was washed three times in TBS-T and incubated at room temperature for 2 hours with the following secondary antibodies goat anti-rabbit IgG H&L (HRP) (Abcam #ab205718, 1:2000) and m-IgG Fc BP-HRP (Santa Cruz ...
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bioRxiv - Cell Biology 2024Quote: The following primary antibodies were used for immunofluorescence (IF) or immunoblotting (IB): rabbit anti-OPTN (Abcam, ab23666; IF, 1:50; IB, 1:1000), mouse anti-GAPDH (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2024Quote: ... Nuclei were incubated in wash buffer containing primary antibody (anti-CRIP2, Abclonal A9038, lot 0050480101; rabbit polyclonal IgG, Abcam ab37415, lot #GR3208186-1) for 1 h ...
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bioRxiv - Bioengineering 2020Quote: ... Sections were blocked with PBS-BSA 5% w/v for 30 min at RT and incubated with the primary antibodies (collagen type II, mouse monoclonal antibody, DSHB, II-II6B3, 1:100; collagen type I, rabbit monoclonal antibody, Abcam, ab138492) overnight at 4°C ...
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bioRxiv - Bioengineering 2019Quote: ... sections were subsequently incubated with the indicated antibodies (rabbit αRanBP9 antibody, 1:50, Sigma-Aldrich, cat. nr. HPA050007; goat αV5 antibody, 1:350, Abcam cat ...
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bioRxiv - Cell Biology 2022Quote: ... The following antibodies were used at 1:500 dilutions: G3BP1 antibody (Abcam ab56574), PABPC1 (abcam ab21060) ...
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bioRxiv - Molecular Biology 2019Quote: Antibodies used in this study are mouse monoclonal TPR-specific antibody (Abcam, ab58344), Nxf1 (rabbit ...
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bioRxiv - Microbiology 2021Quote: ... antibody or a 1:5,000 dilution of α-GFP HRP-conjugated antibody (Abcam). Blots were imaged after incubation with SuperSignal West PICO PLUS Chemiluminescent Substrate (ThermoFisher) ...
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bioRxiv - Cancer Biology 2020Quote: ... Primary antibodies were detected with Alexa Fluor546-conjugated secondary antibodies (Abcam, Cambrige, UK). Images were acquired using a laser scanning confocal microscope (LSM 510 ...
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bioRxiv - Genomics 2022Quote: ... Antibodies were added to each sample (0.5μg of rabbit glucocorticoid receptor antibody [abcam, ab225886 ...
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bioRxiv - Cell Biology 2020Quote: ... Citrate carrier antibody (#99168) and Histone H4 antibody (#31830) were obtained from Abcam.
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bioRxiv - Neuroscience 2020Quote: ... Primary antibodies used: rabbit polyclonal IgG antibody to NeuN (104225, Abcam, 1:100) and mouse monoclonal IgG2A antibody to myelin Proteolipid Protein (clone PLPC1 ...
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bioRxiv - Neuroscience 2020Quote: ... Primary antibodies and dilutions used were: rat antibody to CTIP2 (1:500, Abcam); mouse antibody to SATB2 (1:200 ...
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bioRxiv - Physiology 2022Quote: ... before overnight incubation with primary antibodies (Total OXPHOS Rodent WB Antibody Cocktail: Abcam ab110413 ...
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bioRxiv - Physiology 2021Quote: ... Samples were incubated with primary antibody (rabbit polyclonal antibody to Pax6, ab5790, Abcam) 1:200 in blocking solution over night at 4°C with rocking agitation ...