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Citations for abcam :
8451 - 8500 of 10000+ citations for Anti INSL3 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2021Quote: ... slides were processed in a DAKO autostainer according to the manufacturer’s protocol using the same antibodies or anti-MITF ab12039 (Abcam, Cambridge, UK, 1:1,000). For immunofluorescence staining of KDM5B ...
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bioRxiv - Bioengineering 2020Quote: ... The GP-13C6 complex was identified by adding a polyclonal human anti-IgG antibody conjugated to FITC (Abcam, Cambridge, USA; Cat. No. ab97224). Cell cultures were observed under fluorescence illumination with an optical microscope (Axio Imager M2 microscope (Zeiss ...
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bioRxiv - Neuroscience 2022Quote: ... The CTB signal was detected by combining the RNAScope Multiplex Fluorescent v2 assay with immunofluorescence using a rabbit anti-CTB primary antibody (Abcam, ab34992, 1:200). The integrated RNA protein co-detection workflow was followed using the RNA-Protein Co-detection Ancillary Kit (Advanced Cell Diagnostics ...
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bioRxiv - Molecular Biology 2022Quote: ... in PBST for 1 h at room temperature and then immunoblotted overnight at 4 °C with corresponding primary antibodies: anti-FTO (Abcam, ab124892, 1/1000) or anti-β-Actin (Sigma ...
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bioRxiv - Physiology 2022Quote: ... and then stained for αSMA overnight using rabbit anti-human αSMA antibody at a 1:500 dilution at 4°C (Abcam, San Francisco, CA). The chamber slides were incubated with a goat anti-rabbit secondary antibody (Invitrogen ...
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bioRxiv - Developmental Biology 2022Quote: ... Immunostaining was performed with the following antibodies at 1:50–500 dilutions in blocking solution for 1–2 hours at room temperature: anti-DDDD-K (Abcam, Cambridge, UK, ab1162), anti-GFP (ThermoFisher Scientific ...
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bioRxiv - Genetics 2019Quote: ... Proteins were transferred to a polyvinylidene difluoride membrane and probed with well-characterized monoclonal antibody of anti-Fibrillin 1 (Abcam ab124334, Cambridge, UK).
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bioRxiv - Physiology 2019Quote: ... sections were incubated for 1 h at room temperature with anti-rabbit Alexa Fluor A555 antibody (ab150078, 1:1000, Abcam, Cambridge, United Kingdom). Slides were washed with PBS and coverslipped with DAPI Fluoromount-G (0100-20 ...
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bioRxiv - Immunology 2019Quote: ... The tissue was embedded in paraffin and sections were stained with an antibody against RELM-α (polyclonal rabbit anti-mouse, Abcam, Cambridge, UK). Nuclei were stained with haematoxylin (Merck) ...
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bioRxiv - Developmental Biology 2020Quote: ... cover slips were incubated in primary antibody at 4°C overnight followed by rinses in PBS and one hour incubation with goat anti-mouse IgG (Alexa Fluor 647-conjugated) secondary antibody (Abcam, Cambridge, MA, ab150115) and/or goat anti-rabbit IgG (Alexa Fluor 594-conjugated ...
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bioRxiv - Pathology 2020Quote: ... antigen retrieval at pH 9 was performed using citrate buffer and sections were incubated with the primary antibody rabbit anti‐mouse CD31 (1:50) (ab28364, Abcam, Cambridge, MA, USA) at 4°C overnight ...
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bioRxiv - Pathology 2020Quote: ... antigen retrieval at pH 6 with citrate buffer and the primary antibody rabbit anti-mouse CD11b (1:200) (ab133357, Abcam, Cambridge, MA, USA) and antigen retrieval with 1:20 proteinase K/TE buffer and rat anti-mouse F4/80 (1:100 ...
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bioRxiv - Plant Biology 2019Quote: ... For the localization of total fraction of RNAPII we used mouse 8WG16 antibody (anti-RNA polymerase II CTD repeat YSPTSPS, Abcam, diluted 1:200), according to protocols provided by Kołowerzo-Lubnau et al64 ...
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bioRxiv - Physiology 2019Quote: Cyst sections were blocked and permeabilized with 3% normal donkey serum in PBS with 0.3% Triton X-100 for 2 h and incubated overnight with rabbit anti-beta-III tubulin antibody (ab18207, 1:2000, Abcam, Cambridge, United Kingdom) in 3% normal donkey serum in PBS with 0.3% Triton X-100 at 4°C ...
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bioRxiv - Physiology 2019Quote: ... for 1 h at room temperature and next incubated with primary antibodies (p62, CoxIV: Cell Signaling,3E11, Tomm20:Abcam, UK, ab56783 (anti-mouse) or ab186734 (anti-rabbit) ...
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bioRxiv - Developmental Biology 2020Quote: ... After the incubation with the primary antibodies the embryos and pupal dissections were washed three times for 30 minutes in PBT and incubated with the respective secondary antibodies: Alexa Fluor 488 conjugated Donkey Anti-Goat (abcam Cat. No: ab150129), Alexa Fluor 488 conjugated Goat Anti-Rabbit (ThermoFisher Scientific Cat ...
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bioRxiv - Genetics 2021Quote: ... Samples were then divided into two 250 μl aliquots and stained at room temperature for 1 hr with anti-RPA1 antibodies (rabbit, Abcam ab79398; 1:100) to detect endogenous RPA1 or anti-V5 tag antibodies (mouse ...
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bioRxiv - Physiology 2020Quote: ... diluted in PBTD was added for 2 hours followed by an anti-elastin rabbit polyclonal antibody diluted in PBTD 1:100 (Abcam, Cambridge, UK; ab21610). The samples were incubated with the primary antibody for 18 hours at 4°C followed by 30 minutes washing in PBTD ...
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bioRxiv - Neuroscience 2021Quote: ... the immunofluorescence staining as carried out as described above but adding also a primary antibody for TNFR1 (Rabbit anti-TNFR1, 1:500, product #ab19139, Abcam, Cambridge, MA, USA) and a secondary antibody (Alexa Fluor 594 donkey anti-rabbit antibody ...
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bioRxiv - Synthetic Biology 2021Quote: ... Periplasmic and cytoplasmic fractions were then run on SDS-PAGE gels and Western blotting was performed according to standard protocols using the following antibodies: anti-FLAG-HRP (Abcam ab49763; 1:2,500), anti-PhoA (Millipore MAB1012 ...
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bioRxiv - Biochemistry 2021Quote: ... then incubated in 5% milk in TBS-T containing 1:10000 horseradish peroxidase (HRP) conjugated goat anti-mouse secondary antibody (Abcam ab6789-1 MG) for 1 hour at room temperature ...
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bioRxiv - Neuroscience 2020Quote: 5-FU and wdr12 samples were immunolabeled with anti-HuC/D antibodies (not shown) (ab210554, abcam, used at a dilution of 1:600). Anti-GFP (GFP-1020 ...
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bioRxiv - Bioengineering 2021Quote: ... This was followed by a 2-hour incubation with secondary antibody goat anti-rabbit Alexa Fluor 488 (1:500, #ab150077, Abcam, Waltham, MA, USA), and a 0.1% (w/v ...
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bioRxiv - Immunology 2021Quote: ... Reacted mAbs were detected using ALP-conjugated Goat F(ab’)2 Anti-Human IgG (Fab’)2 secondary antibody conjugated with ALP (Abcam, ab98532, 1:2000) for 30 mins at RT ...
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bioRxiv - Immunology 2021Quote: ... The ELISA plates were washed 4 times by blocking buffer and 50 μL Goat F(ab’)2 Anti-Human IgG (Fab’)2 secondary antibody conjugated with ALP (Abcam, ab98532, 1:2000) was incubated with the plate at RT for 30 mins ...
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bioRxiv - Genomics 2021Quote: ... Secondary antibodies used in this study were as follows: goat anti-rabbit IgG Alexa Fluor 555 (1:500 dilution; abcam; Cat. No. ab150078), goat anti-mouse IgG Alexa Fluor 488 (1:500 dilution ...
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bioRxiv - Neuroscience 2022Quote: ... Sections were incubated at room temperature for ~3 hours in secondary antibody solution containing donkey anti-rabbit IgG H&L Texas Red (abcam, Cambridge, UK; ab7081) and donkey anti-goat IgG H&L Alexa Fluor 488 (abcam ...
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bioRxiv - Neuroscience 2022Quote: ... for 5 minutes each and incubated in the dark with their corresponding secondary antibody: Goat anti-rabbit Alexa 647 (1:1000 dilution, Abcam, Waltham, MA, USA) and Goat anti-mouse Alexa 594 (1:1000 dilution ...
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bioRxiv - Neuroscience 2022Quote: ... Sections were incubated with primary antibodies (ON; 4°C) that were appropriately diluted in blocking solution: chicken anti-GFP (Abcam; ab13970; 1:500), rabbit anti-GFP (Santa Cruz Biotechnology ...
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bioRxiv - Neuroscience 2022Quote: ... The brain sections of the rats with Gi-DREADDs were incubated first in a blocking solution (10% fetal horse serum and 0.15% Triton X-100 in PBS) for 1 h at 24 ± 1°C and then with primary rabbit anti-mCherry antibody (1:1000, Abcam, ab167453, Cambridge, UK) overnight at room temperature ...
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bioRxiv - Microbiology 2022Quote: ... Slides were washed with PBS thrice and NP antigen was detected using a goat anti-influenza NP polyclonal antibody (abcam catalogue number ab155877) for 1 h ...
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bioRxiv - Plant Biology 2024Quote: ... Protein expression of rSPL9-GFP was verified by SDS-PAGE and immunoblotting using an anti-GFP antibody (Abcam, Cat# ab290, 1:10,000 dilution) and HRP-conjugated secondary antibody (Promega ...
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bioRxiv - Cancer Biology 2024Quote: Immunofluorescence (IF) was performed on whole FFPE 5-µm-thick tissue sections using anti-TP63 mouse monoclonal antibody (1:100; Abcam, catalog no. ab735), anti-CK8 rabbit polyclonal antibody (1:100 ...
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bioRxiv - Immunology 2023Quote: ... cells that were stained with anti-AMPKα1 antibody and unconjugated isotype control antibody were incubated in the dark at room temperature for 2 hours with anti-rabbit Alexa Fluor 488 secondary antibody (Abcam cat. no. ab150113). Following antibody incubation ...
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bioRxiv - Neuroscience 2024Quote: ... blocked for 1 hour with 10% serum in PBS and incubated overnight at 4LC with the following primary antibodies in PBS: chicken anti-MAP2 (Abcam, ab92434, 1:500), rabbit anti- tyrosine hydroxylase (TH ...
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bioRxiv - Molecular Biology 2024Quote: ... Coverslips were incubated with the following primary and secondary antibodies in PBS/10 % FBS for 1 hour to overnight: mouse anti-MRE11 (abcam, #ab214, 1:500), rabbit anti-TFAM (antibodies-online ...
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bioRxiv - Microbiology 2024Quote: ... Antigen-specific IgG antibodies in hamster sera were detected using horseradish peroxidase (HRP)-labeled goat anti-Syrian hamster IgG H+L Ab (Abcam, 1/30000 dilution). IgG1 and IgG2/3 subclass Abs were analyzed using biotinylated subclass-specific antibodies (Southern Biotech ...
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bioRxiv - Neuroscience 2024Quote: ... Tween-20 in tris-buffered saline (TBS-T) then incubated overnight at 4°C with one of the following primary antibodies: rabbit anti-GluK4 (Abcam; ab10101, 1:500), rabbit anti αB-crystallin (Enzo Life Sciences ...
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bioRxiv - Developmental Biology 2024Quote: ... Samples were incubated in blocking solution for 2 hr and then incubated with goat anti-rabbit-HRP secondary antibody (Abcam; ab6721; 1:300) overnight at 4°C ...
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bioRxiv - Microbiology 2023Quote: ... Midguts were washed five times via direct tube transfer in PBS+T before staining with the secondary antibody (Goat anti-mouse Alexa Fluor 594, Abcam, Cambridge, United Kingdom) at a 1:1000 dilution in blocking solution for 1 hour at room temperature ...
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bioRxiv - Cancer Biology 2024Quote: ... The lungs were harvested and cryosections were prepared for immunofluorescence (IF) staining with an anti-BrdU antibody (Abcam, Cambridge, UK; Catalogue no. ab70328). Images were acquired and analysed using an Applied Spectral Bioimaging System (ASI ...
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bioRxiv - Cancer Biology 2024Quote: ... The epithelial nature of the primary cultures and single-cell clones were analysed using a recombinant anti-EpCAM antibody (EPR20533-63; Catalogue number: ab221552, Abcam; Supplementary Figure 2).
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bioRxiv - Microbiology 2024Quote: ... The equivalent of 0.3 OD units was analyzed by SDS-PAGE and western blot using the following antibodies and dilutions: mouse anti-AD (Abcam, cat nr ab135398) 1:200 in 3% BSA in PBS-0.5% Tween (PBS-T) ...
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bioRxiv - Cancer Biology 2024Quote: ... Formalin-fixed paraffin embedded tissues from 10 NSCLC patient-derived xenograft (PDX) models were processed for immunohistochemistry to detect centrosomes using the anti-pericentrin antibody ab4448 (Abcam, 1:1000 dilution). Mitotic cells were classified as having CA if they contained more than 2 centrosomes and the proportion with CA was calculated for each tumor ...
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bioRxiv - Bioengineering 2024Quote: ... Incubation with the primary antibody was carried out at 4°C overnight in a PBS (1x) solution containing anti-Ki67 (Abcam, MA, 1:100), BSA (1%) ...
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bioRxiv - Genomics 2023Quote: ... cells were immunostained in blocking buffer containing mouse anti-beta III Tubulin (Tub) conjugated Alexa Fluor 488 antibody (Abcam ab195879, dilution 1:1000) and DAPI (Sigma D9542 ...
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Mucociliary Clearance Augmenting Drugs Block SARS-Cov-2 Replication in Human Airway Epithelial CellsbioRxiv - Microbiology 2023Quote: ... while sections of caspase and protein S staining were incubated with goat anti-rabbit IgG secondary antibody conjugated with HRP (Abcam ab6721, 1:1,000). ImmPACT DAB Peroxidase (HRP ...
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bioRxiv - Genomics 2022Quote: ... The cells were washed three times with PBS and resuspended with 2% BSA-PBS containing a goat anti-rabbit IgG FITC conjugated secondary antibody (Abcam catalog no. ab6717) and incubated for 30 minutes on ice in the dark ...
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bioRxiv - Molecular Biology 2023Quote: ... The membrane was blocked with 5% skim milk for 1 hour at room temperature and then were incubated overnight at 4°C with the anti-MGST3 antibody (Abcam, ab192254; 1: 1000). Subsequently it was incubated with horseradish peroxidase (HRP)-conjugated secondary antibody (Cell signaling ...
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bioRxiv - Cell Biology 2023Quote: ... 1:400 dilution) and anti-α- synuclein aggregate antibody (Clone#: MJFR-14-6-4-2; 1:1000 dilution) were acquired from Abcam (Cambridge UK).