Tetratricopeptide repeat protein 14 TTC14 Antibody HRP citations for abcam

CDK12 Loss Promotes Prostate Cancer Development While Exposing Vulnerabilities to Paralog-Based Synthetic Lethality

Jean Ching-Yi Tien, et al., bioRxiv - Cancer Biology 2024

Quote: ... anti-human antibodies were used for the following proteins: CDK12 (Cell Signaling 11973S, Abcam ab246887), β-Actin (Santa Cruz sc47778) ...

RAPSYN-Mediated Neddylation of BCR-ABL Alternatively Determines the Fate of Philadelphia Chromosome-positive Leukemia

Mengya Zhao, et al., bioRxiv - Cancer Biology 2024

Quote: ... Protein expression levels were analyzed by immunoblotting with the antibodies of anti-RAPSYN (ab118491, Abcam) or SRC (11097-1-AP ...

FoxA1/2-dependent epigenomic reprogramming drives lineage switching in lung adenocarcinoma

Katherine Gillis, et al., bioRxiv - Cancer Biology 2023

Quote: ... Chromatin from all samples was immunoprecipitated with antibodies premixed with Protein A Dynabeads: FoxA1 (Abcam, ab170933 ...

Intercalation of the disulfide bond between the A2 and A4 loop of Cellobiohydrolase (Cel7A) of Aspergillus fumigatus enhances catalytic activity and thermostability

Subba Reddy Dodda, et al., bioRxiv - Biochemistry 2023

Quote: ... Western blot further confirmed the purified protein with an anti-His monoclonal antibody (Abcam, USA).

Evolution of chromosome arm aberrations in breast cancer through genetic network rewiring

Elena Kuzmin, et al., bioRxiv - Genomics 2023

Quote: ... 1500µg of protein was then incubated with either 1.8μl (∼5µg) V5-tag antibody (Abcam, ab27671), 1.25µl (∼5µg ...

Apo- and holo- transferrin differentially interact with ferroportin and hephaestin to regulate iron release at the blood-brain barrier

Stephanie L. Baringer, et al., bioRxiv - Cell Biology 2023

Quote: ... Primary antibodies used were the following: myelin basic protein 1 (MBP1, Abcam, ab22460, 1:500), ferritin (Abcam ...

Characterization of mitotic phenotypes associated with a MYC synthetic lethal compound

Qiong Shi, et al., bioRxiv - Cancer Biology 2023

Quote: ... A rabbit polyclonal antibody recognizing the protein Ninein/NIN was obtained from Abcam (Cat. #ab4447). Bub1 mouse monoclonal antibody (Cat ...

Opi1-mediated transcriptional modulation orchestrates genotoxic stress response in budding yeast

Giovanna Marques Panessa, et al., bioRxiv - Cell Biology 2023

Quote: ... Rad53 and epitope tagged proteins were probed using specific antibodies: anti-gamma H2A (Ab17353-Abcam, 1:2500 dilution in TBST and 5% non-fat dry milk) ...

Dysfunctional S1P/S1PR1 signaling in the dentate gyrus drives vulnerability of chronic pain-related memory impairment

Mengqiao Cui, et al., bioRxiv - Neuroscience 2024

Quote: ... 30 μl Protein A beads were mixed with 5μg/mL anti-S1PR1 antibody (Abcam, Ab259902) and incubated with gentle rotation at room temperature for 15 min ...

Chemotherapy activates inflammasomes to cause inflammation-associated bone loss

Chun Wang, et al., bioRxiv - Cell Biology 2024

Quote: ... Proteins were transferred onto nitrocellulose membranes and incubated with antibodies against GSDMD (1;1,000; Abcam, MA), GSDME (1;1,000 ...

ZNFX1 is a Novel Master Regulator in Epigenetically-induced Pathogen Mimicry and Inflammasome Signaling in Cancer

Lora Stojanovic, et al., bioRxiv - Cancer Biology 2024

Quote: ... The following antibodies were employed to determine protein abundance: anti-ZNFX1 (1:1000, Abcam, #ab179452), anti-MAVS (1:1000 ...

The structural basis for kinetochore stabilization by Cnn1/CENP-T

Stephen M. Hinshaw, Stephen C. Harrison, bioRxiv - Cell Biology 2020

Quote: ... Western blotting was carried out as described (Hinshaw et al., 2019) with the following antibodies: anti-FLAG-HRP (Abcam 49763 from ascites), anti-PGK1 (Invitrogen 459250) ...

The nitrogen phosphotransferase regulator PtsN (EIIA^Ntr) regulates inorganic polyphosphate production in Escherichia coli

Marvin Q. Bowlin, et al., bioRxiv - Microbiology 2021

Quote: ... Blots were washed again in 3X TBS-T then incubated in blocking buffer containing a 1:5000 dilution of rabbit Anti-DDDDK HRP conjugated antibody (Abcam Cat #ab2493). Blots were washed three times with TBST and once with TBS ...

Role of autophagy in sepsis-induced skeletal muscle dysfunction, whole-body metabolism, and survival

Jean-Philippe Leduc-Gaudet, et al., bioRxiv - Cell Biology 2021

Quote: ... Membranes were blocked in PBS + 1% Tween® 20 + 5% BSA for 1 h at room temperature then incubated overnight with specific primary antibodies at 4°C.Membranes were washed in PBST (3×5 min) then incubated with HRP-conjugated goat anti-rabbit (ab6721) or rabbit anti-mouse (ab6728) secondary antibodies (Abcam, Cambridge, MA) for 1 h at room temperature ...

Role of Rv3351c in trafficking Mycobacterium tuberculosis bacilli in alveolar epithelial cells and its contribution to disease

Megan Prescott, et al., bioRxiv - Microbiology 2020

Quote: ... the membrane was washed three times with Tris-buffered saline containing 0.01% Tween 20 and incubated for one hour with secondary mouse HRP-conjugated antibody (Abcam Inc., Cambridge, MA). All incubations and wash steps were performed at room temperature unless otherwise stated ...

mir-21 is associated with inactive low molecular weight Argonaute complexes in thyroid cancer cell lines

Bonita H. Powell, et al., bioRxiv - Molecular Biology 2020

Quote: ... membranes were incubated with secondary antibody for 1 hr at room temperature: rat-HRP 1:10,000 dilution (abcam ab205720 Lot# GR32115155-1), rabbit-HRP 1:10,000 dilution (Santacruz sc-2004 Lot#B2216) ...

Optimizing the PBS1 Decoy System to Confer Resistance to Potyvirus Infection in Arabidopsis and Soybean

Sarah E. Pottinger, et al., bioRxiv - Plant Biology 2020

Quote: ... Membranes were washed 3 times for 15 mins in 1X Tris-buffered saline (TBS) with 0.1% Tween20 (TBST) and incubated with HRP-conjugated goat anti-mouse antibody (abcam, catalog number ab6789). All antibodies were used at a concentration on 1:5000 ...

Ribosome display of N-linked glycoproteins in cell-free extracts

Sean S. Chung, et al., bioRxiv - Synthetic Biology 2022

Quote: ... 0.1% Tween 20) and subsequently probed for 1 h with one of the following: horseradish peroxidase (HRP)-conjugated anti-DDDDK antibody (Abcam, cat # ab49763) that recognized the FLAG epitope tag ...

Gadolinium-based nanoparticles AGuIX and their combination with ionizing radiation trigger AMPK-dependent proinflammatory reprogramming of tumor-associated macrophages

Zeinaf Muradova, et al., bioRxiv - Immunology 2024

Quote: ... anti-beta Actin HRP [AC-15] (#ab49900, diluted at 1:10000), anti-IL-1 beta (#ab2105, diluted at 1:1000) antibodies from Abcam (Cambridge, UK), anti-AMPKα2 (#AF2850 ...

Screening Peptide Drug Candidates to Neutralize Whole Viral Agents : A Case study with SARS-CoV-2 Virus

Cemile Elif Özçelik, et al., bioRxiv - Bioengineering 2023

Quote: ... which was prepared by 5% milk in TBS-T containing 1:10,000 horseradish peroxidase (HRP)-conjugated goat anti-mouse secondary antibodies (Abcam ab6789-1 MG). The secondary antibody incubation was applied for 1 hour at room temperature ...

Mucociliary Clearance Augmenting Drugs Block SARS-Cov-2 Replication in Human Airway Epithelial Cells

Javier Campos-Gomez, et al., bioRxiv - Microbiology 2023

Quote: ... sections corresponding to tubulin staining were incubated with a goat anti-mouse IgG secondary antibody conjugated with HRP (Abcam ab6789, 1:500), while sections of caspase and protein S staining were incubated with goat anti-rabbit IgG secondary antibody conjugated with HRP (Abcam ab6721 ...

A Bacterial Living Therapeutics with Engineered Protein Secretion Circuits To Eliminate Breast Cancer Cells

Gozeel Binte Shahid, et al., bioRxiv - Synthetic Biology 2023

Quote: ... The membrane was washed with TBS-T and incubated for 1 hour at room temperature with a 1:10,000 dilution of HRP-conjugated goat anti-mouse secondary antibodies (Abcam ab6789-1 MG) in 5% milk-TBS-T ...

Exploiting Epigenetic Targets to Overcome Taxane Resistance in Prostate Cancer

Buse Cevatemre, et al., bioRxiv - Cancer Biology 2023

Quote: ... The membranes were washed with TBST and incubated with the HRP-conjugated secondary antibodies (Goat pAb to Rabbit and Mouse IgG, Abcam, ab205718, ab205719) for 1 h at rt ...

Acetylation-dependent coupling between G6PD activity and apoptotic signaling

Fang Wu, et al., bioRxiv - Biochemistry 2023

Quote: The following secondary antibodies have been used for Western blotting: Goat Anti-Mouse IgG H&L (HRP) preadsorbed (Abcam, #ab7068; 1:10000), Recombinant Protein G (HRP ...

Comprehensive analysis of nasal IgA antibodies induced by intranasal administration of the SARS-CoV-2 spike protein

Kentarou Waki, et al., bioRxiv - Immunology 2023

Quote: ... The plates were washed three times with PBST and then incubated with anti-mouse IgG or IgA secondary antibody conjugated to horseradish peroxidase (HRP) (Abcam, Cambridge, UK) in PBST for 1 h at room temperature ...

Human ADA2 deficiency is characterized by the absence of an intracellular hypoglycosylated form of adenosine deaminase 2

Lisa Ehlers, et al., bioRxiv - Immunology 2024

Quote: ... The membranes were washed and incubated with the respective HRP-coupled secondary antibodies for one hour at room temperature: goat anti-rabbit IgG H&L (#ab205718, 1:5000; abcam; RRID: AB_2819160), Goat Anti-Mouse IgG (H + L ...

Highly sensitive in situ proteomics with cleavable fluorescent tyramide reveals human neuronal heterogeneity