Labshake search
Citations for abcam :
801 - 850 of 10000+ citations for Ribonuclease P Protein Subunit p38 RPP38 Antibody Biotin since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... The cells were then probed for 1 h with a polyclonal rabbit anti-SARS-CoV-2-S protein antibody (1:2,250; ab252690; Abcam, Cambridge, UK) or a rabbit anti-MeV N protein antibody (1:1,000 ...
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bioRxiv - Microbiology 2022Quote: ... Spain) binding to the 229E nucleocapsid protein and a goat anti-mouse secondary antibody conjugated with fluorescein isothiocyanate (Abcam, United Kingdom). TCID50 titers were calculated according to the method of Reed and Muench (44).
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bioRxiv - Microbiology 2020Quote: ... endogenous human NPC1 and HSP90 were purified using immobilized Recombinant Protein G Resin (Generon) and 4 µg of specific antibodies against NPC1 (Abcam, ab108921) or HSP90 (Enzo Life Sciences ...
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bioRxiv - Microbiology 2020Quote: ... 50 μg of recombinant Rv3351c protein and anti-6xHIS antibody conjugated to horseradish peroxidase (HRP, 1:500; Abcam Inc., Cambridge, MA) were mixed together in PBS containing 0.5% BSA ...
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bioRxiv - Cancer Biology 2021Quote: Protein extraction and immunoblotting was performed according to standard procedures with primary antibodies against FLI1 (1:500, rabbit, ab15289, abcam, UK) and GAPDH (1:5000 ...
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bioRxiv - Cell Biology 2022Quote: ... deparaffinized sections were rehydrated and stained using specific antibodies against Leucine-rich repeat-containing G-protein-coupled receptor 5 (Lgr5, ab219107, Abcam, USA), Lysozyme (ab76784 ...
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bioRxiv - Neuroscience 2020Quote: ... The proteins of interests were detected using the following primary antibodies: anti-rabbit Catalase (1:375; catalog #Ab52477; Abcam, Cambridge, MA); anti-rabbit Glutathione Peroxidase (1:500 ...
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bioRxiv - Physiology 2021Quote: ... Membranes were blocked in 3% skim milk for 2 hours and then incubated with primary antibody overnight at 4°C for the following proteins: 4HNE (Abcam, ab46545), β-actin (Santa Cruz Biotech) ...
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bioRxiv - Neuroscience 2021Quote: ... constant shaking) with the following primary antibodies: sheep anti-phospo-S6 ribosomal protein (pS6; 1:250, ab65748, Abcam, Cambridge, Massachusetts, USA); mouse anti-D3R (1:150 ...
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bioRxiv - Neuroscience 2021Quote: ... in PBS for 1 hour then incubated overnight with Anti-Amyloid beta precursor protein (Y188) rabbit monoclonal antibody at 1:1,000 (Abcam Cat# ab32136) and Anti-VCP mouse monoclonal antibody at 1:2,000 (Abcam Cat# ab11433) ...
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bioRxiv - Microbiology 2021Quote: ... Proteins co-purified with His-NS5A or His tag protein were detected by western blot with mouse anti-Myc tag monoclonal antibody (Abcam, USA) for probing NEMO and mouse anti-His tag monoclonal antibody (Abcam ...
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bioRxiv - Genomics 2021Quote: ... Fragmented DNA was then diluted 10 times with ChIP dilution buffer and protein-DNA complexes were captured using an H3K27ac antibody (Abcam AB4729). Next ...
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bioRxiv - Cancer Biology 2020Quote: S-nitrosylation of proteins was detected by western blot using an S-nitrosylation specific antibody (HY8E12 clone, Abcam # 94930, 1:20) or the Pierce S-Nitrosylation Western Blot detection kit (Thermo Fisher Scientific # 90105) ...
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bioRxiv - Immunology 2022Quote: ... The slides were incubated with primary rabbit anti-SARS nucleocapsid protein antibody(NB100-56576, Novus, USA) overnight at 4°C and then with goat anti-rabbit IgG HRP (Abcam, UK) for 1 hr ...
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bioRxiv - Neuroscience 2023Quote: ... After SDS-PAGE and proteins transferred on PVDF membranes were incubated overnight at 4°C with Total OXPHOS Human WB Antibody Cocktail (Abcam, UK) and subsequently detected with HRP-conjugated goat anti-rabbit IgG secondary antibody (1:4000 ...
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bioRxiv - Neuroscience 2022Quote: ... NF200 (1:500, Chemicon International, Temecula, CA, USA) rabbit monoclonal amyloid precursor protein (APP) antibody [Y188] (1:500, Abcam, Cambridge, MA) and NeuN (1:500 ...
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bioRxiv - Genomics 2022Quote: ... The ChIP assay was performed with Dynabead Protein G beads and histone antibodies (H3K4me3: Millipore #07-473, H3K4me1: Millipore #07-436, H3K27me3: Millipore #07-449, H4K27ac: abcam ab4729). After binding to antibodies ...
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bioRxiv - Physiology 2023Quote: ... Frozen sections were fixed with fresh 4% paraformaldehyde and neutrophil elastase protein was detected using anti-neutrophil elastase antibody (Abcam #68672).
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bioRxiv - Developmental Biology 2023Quote: ... nucleoplasm and chromatin fractions were used for protein analysis with western blotting with the following antibodies were used: anti-DHX9 (Abcam 26271), anti-TBP (Abcam 818) ...
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bioRxiv - Cancer Biology 2022Quote: Protein array analysis was performed using the Human MAPK Phosphorylation Antibody Array (ab211061) according to the manufacturer’s instructions (Abcam, Boston, MA). Visualization was performed with the ChemiDoc Touch Imaging System (Bio-Rad ...
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bioRxiv - Genomics 2023Quote: ... The heterozygous expression of the Dam-Lamin B1 fusion protein was further confirmed by Western blot using an anti-HA antibody at 1 in 5000 dilution (Abcam, ab9110) and an anti-LaminB1 antibody at 1 in 5000 dilution (Abcam ...
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bioRxiv - Neuroscience 2023Quote: ... the cryosections of human brain were mounted on a Polyethylene naphthalate (PEN) Membrane Slide (Zeiss, 415190-9041-000) and then stained with spike protein antibodies using the IHC kit (Abcam, ab210062). After further processing ...
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bioRxiv - Neuroscience 2023Quote: ... CTIP2 (chicken ovalbumin upstream promoter transcription factor-interacting protein) antibody was added (rat, 1:500, Abcam, Cat: ab18465, Lot: GR3272266-13) to facilitate delineation of striatum and incubation continued until the 4 hours were completed ...
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bioRxiv - Molecular Biology 2024Quote: ... previously blotted membranes from Figure S2C,E were re-probed with 1:5000 mouse monoclonal anti-TATA binding protein antibody (Abcam #ab51841). The membrane in Figure S2H was probed for 1 h with 1:1000 rabbit anti-LARP7 antibody (Fortis/Bethyl #A303-723A ...
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bioRxiv - Microbiology 2024Quote: ... and a rabbit antibody recognizing cellular proteins or epitope tags (anti-EEA1, Cell Signaling Technology, 2411, 1:75; anti-TGN46, Abcam, ab50595 ...
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bioRxiv - Molecular Biology 2024Quote: ... The membrane was then probed with a polyclonal rabbit anti-human antibody targeting human AL light chain protein (Abcam, 1:500). A horseradish peroxidase-conjugated goat anti-rabbit IgG (Invitrogen ...
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bioRxiv - Immunology 2024Quote: ... and stained for proteins of interest using the following primary and secondary antibodies: S100A8 (clone: EPR3554, 1:100, Abcam, cat# ab92331), goat anti-rabbit IgG Alexa Fluor 594 (1:500 ...
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bioRxiv - Biophysics 2024Quote: ... Nuclear pore complex protein Nup133 or β-tubulin were labeled overnight at 4°C with rabbit anti-Nup133 antibody (Abcam, #ab155990) and mouse anti-human β-tubulin mAb (Sigma-Aldrich ...
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bioRxiv - Biochemistry 2024Quote: ... Eluted proteins were resolved on NuPAGE™ 4–12% Bis–Tris gel followed by Western blotting using anti-IFNAR1 antibody (ab124764, Abcam).
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bioRxiv - Cell Biology 2024Quote: ... SR-BI protein was determined by Western blotting by probing cell lysates with SR-BI antibody (ab52629, Abcam, 1:1000 dilution).
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bioRxiv - Plant Biology 2024Quote: ... The proteins were also visualised by total protein staining with Instant Blue® Coomassie Protein Stain (Abcam ab119211). For Western blot analysis ...
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bioRxiv - Physiology 2020Quote: ... Protein concentrations were measured using BCA Protein Assay Reagent (Abcam, USA). Proteins were separated on SDS-PAGE and transferred to the polyvinylidene fluoride (PVDF ...
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bioRxiv - Bioengineering 2024Quote: ... Protein mass was determined using the BCA protein assay kit (Abcam) with PierceTM bovine serum albumin as a standard (Thermo Scientific).
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bioRxiv - Cell Biology 2020Quote: ... rabbit anti-biotin (ab53494, WB 1:1000), mouse anti-integrin beta 1 (ab30394, IF 1:400) all from Abcam; rabbit anti-Rabankyrin-5 was a gift from Professor Marino Zerial (Max Planck Institute of Molecular Cell Biology and Genetics ...
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Development of monoclonal antibody-based blocking ELISA for detecting SARS-CoV-2 exposure in animalsbioRxiv - Microbiology 2023Quote: ... Biotinylation of the mAb was performed using a Biotin Conjugation Kit by following the manufacturer’s instruction (Abcam, Cambridge, MA). The SARS-CoV-2 N-specific mAb B6G11 was previously developed in Diel lab (20).
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bioRxiv - Cancer Biology 2021Quote: ... Equal amounts of total proteins as determined by BCA Protein Assay were analyzed by SDS-polyacrylamide gel electrophoresis with antibodies specific to IL-8 (Cat. No. 18672, Abcam, Cambridge, MA) and prostate specific antigen (Cat ...
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bioRxiv - Neuroscience 2021Quote: ... slices were then incubated at 4 °C overnight in the primary antibodies: chicken anti-glial fibrillary protein GFAP (targeting astrocytes, 1:200, Abcam #ab4674, USA), goat anti-ionized calcium binding adaptor molecule 1 (Iba1 ...
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bioRxiv - Neuroscience 2021Quote: ... slices were then incubated at 4 °C for 5-7 days in the primary antibodies containing: chicken anti-glial fibrillary acidic protein (GFAP) (targeting astrocytes, 1:200, Abcam #ab4674, USA) and/or and NeuN (targeting nuclei of neurons ...
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bioRxiv - Neuroscience 2021Quote: ... the brain slides were incubated with primary antibody rabbit monoclonal anti-amyloid precursor protein (APP) [clone Y188] (#AB32136, Abcam, USA; 1:500) in blocking solution at 4 °C overnight ...
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bioRxiv - Molecular Biology 2021Quote: ... The membrane was then washed thrice with 1X-TBST and incubated with anti-SARS-CoV-2 N protein antibodies (catalogue No. ab272852, Abcam, 1:1000) in TBST with BSA (2% ...
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bioRxiv - Cell Biology 2021Quote: ... The cells were then fixed with 4% paraformaldehyde and processed for immunofluorescence analysis using GAP SH3 Binding Protein 1 (G3BP1) antibody (green; Abcam, Cambridge, MA) and fluorophore-conjugated secondary antibody ...
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bioRxiv - Animal Behavior and Cognition 2022Quote: ... Astrogliosis was visualized after staining with rabbit Monoclonal Anti-Glial Fibrillary Acidic Protein (GFAP) antibody at a 1:1000 dilution (Abcam, Cambridge, MA). All samples were analyzed on an inverted epifluorescent microscope (Olympus IX71) ...
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bioRxiv - Neuroscience 2020Quote: ... Slices were incubated over night with primary antibody rabbit-anti-Ca2+/calmodulin-dependent protein kinase II (1:200, #PA5-38239, Thermo Fisher, MA, USA; 1:500, #ab52476, Abcam, UK), rabbit-anti-parvalbumin (1:500 ...
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bioRxiv - Biochemistry 2021Quote: ... and analyzed by western blot using anti-HBP1 (11746-1-AP, Protein Tech Group, 1:500) or anti-ZMYND19 antibody (ab86555, Abcam, 1:500).
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bioRxiv - Neuroscience 2020Quote: Lysates from DRG neurons were run on 4-12% Bis-Tris gradient gels and proteins were transferred to PVDF membranes that were incubated with antibodies to REST (Abcam, 1:1000), using anti-β-actin as a loading control ...
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bioRxiv - Pathology 2021Quote: ... tissue proteins (20 μg) were separated by 4-20% SDS-PAGE and immunoblots were developed using antibodies against pSMAD3 (Abcam, 1:1000), SMAD3 (Abcam ...
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bioRxiv - Biochemistry 2021Quote: ... The homogenate was clarified by centrifugation at 21,130 x g for 15 minutes before analysis of protein levels in the supernatant by immunoblotting using antibodies including anti-PKA pRIIα (Abcam, RRID: AB_779040), anti-GFP (Sigma Aldrich ...
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bioRxiv - Immunology 2021Quote: ... Cells were then stained for intracellular spike protein using an Anti-SARS-CoV-2 Spike Glycoprotein S1 antibody (Abcam, ab275759, 1:50) followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor 488 ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... a pair of monoclonal antibodies (clone EPR16588, ab178846; clone EPR16589, ab178847) and a recombinant human protein (ab105593) are available from Abcam (Waltham, MA). Similarly ...
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bioRxiv - Microbiology 2021Quote: ... was used for indirect immunofluorescence microscopy at a dilution of 1:300 and mouse monoclonal antibody against nuclear pore complex proteins (Abcam, Cambridge, MA) was used for indirect immunofluorescence microscopy at a dilution of 1:500 ...