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• Antipsychotic-like effects of the selective Rho-kinase 2 inhibitor KD025 in genetic and pharmacological mouse models of schizophrenia

  Rinako Tanaka, et al., bioRxiv - Pharmacology and Toxicology 2024

  Quote: Serum prolactin concentrations were assessed with the Mouse Prolactin ELISA Kit (ab100736; Abcam, Cambridge, UK) using an overnight incubation protocol36 ...
• “RIG-I mediated neuron-specific IFN type 1 signaling in FUS-ALS induces neurodegeneration and offers new biomarker-driven individualized treatment options for (FUS-)ALS.”

  Marcel Naumann, et al., bioRxiv - Neuroscience 2024

  Quote: ... Secondary antibodies (anti-rabbit IRDye 800CW abcam Ab216773, anti-mouse IRDye 800CW abcam ab216772, anti-rabbit IRDye® 680RD abcam ab216777, anti-mouse IRDye® 680RD abcam ab216776) were incubated in TBS-T overnight at 4°C ...
• “RIG-I mediated neuron-specific IFN type 1 signaling in FUS-ALS induces neurodegeneration and offers new biomarker-driven individualized treatment options for (FUS-)ALS.”

  Marcel Naumann, et al., bioRxiv - Neuroscience 2024

  Quote: ... Secondary antibodies (anti-rabbit IRDye 800CW abcam Ab216773, anti-mouse IRDye 800CW abcam ab216772 ...
• Chronic β3 adrenergic agonist treatment improves brain microvascular endothelial function and cognition in aged mice

  Duraipandy Natarajan, et al., bioRxiv - Neuroscience 2024

  Quote: ... Anti-GLUT1 antibody (Abcam #ab115730) was loaded at 1:50 dilution ...
• Academic stress through salivary biomarkers: A multivariate analysis of cortisol, IL-1β, CRP, and IgA levels and their variations as a function of sex

  Rodrigo Castillo Klagges, et al., bioRxiv - Physiology 2024

  Quote: ... IgA (Human IgA ELISA Kit Abcam, Waltham, MA, United States). A microplate reader (BioTek Instruments EL800 ...
• Academic stress through salivary biomarkers: A multivariate analysis of cortisol, IL-1β, CRP, and IgA levels and their variations as a function of sex

  Rodrigo Castillo Klagges, et al., bioRxiv - Physiology 2024

  Quote: ... IL-1β (Human IL-1 beta ELISA Kit Abcam, Waltham, MA, United States), PCR (Human C Reactive Protein ELISA Kit Abcam ...
• Endurance exercise with reduced muscle glycogen content influences substrate utilization and attenuates acute mTORC1- and autophagic signaling in human type I and type II muscle fibers

  O Horwath, et al., bioRxiv - Physiology 2024

  Quote: ... or MyHC II (Abcam #ab91506, diluted 1:10,000). A representative immunoblot is presented in Figure 1D.
• Rac1 inhibition prevents axonal cytoskeleton dysfunction in Transthyretin Amyloid Polyneuropathy

  Joana Magalhães, et al., bioRxiv - Neuroscience 2024

  Quote: ... Membranes were probed overnight at 4°C with primary antibody mouse anti-Rac1 (1:2,000; Abcam, ab33186) and subsequently with the secondary antibody anti-mouse IgG-HRP (1:10,000 ...
• Rac1 inhibition prevents axonal cytoskeleton dysfunction in Transthyretin Amyloid Polyneuropathy

  Joana Magalhães, et al., bioRxiv - Neuroscience 2024

  Quote: ... Samples were resolved on a 12% SDS PAGE gel followed by standard western blot and Ponceau S staining to confirm uniform pull-downs before detection with the relevant antibodies: mouse anti-Rac1 (1:500; Abcam, ab33186) and mouse anti-GAPDH (1:100,000 ...
• Low dose of a non-urea selective GIRK channel activator improves hippocampal-dependent synaptic plasticity and memory disrupted by amyloid-β oligomers

  Jaime Mulero-Franco, et al., bioRxiv - Neuroscience 2024

  Quote: ... injection of oAβ1-42 (1 μg/μL; 0.15 μg/g; monomeric Aβ1-42 #ab120301; Abcam, UK; Fig. 1B) as previously described [40] ...
• Targeted H3K9 acetylation at lncSox1 promoter by cell type-specific epigenome editing promotes intermediate progenitor proliferation in developing mouse cortex

  Godwin Sokpor, et al., bioRxiv - Neuroscience 2024

  Quote: Monoclonal (mAb) and polyclonal (pAb) primary antibodies were commercially sourced and listed as follows: GFP chick pAb (1:400; Abcam), mCherry/RFP pAb (1:1000 ...
• Targeted H3K9 acetylation at lncSox1 promoter by cell type-specific epigenome editing promotes intermediate progenitor proliferation in developing mouse cortex

  Godwin Sokpor, et al., bioRxiv - Neuroscience 2024

  Quote: ... TBR2 rabbit pAb (1:200; Abcam), H3K9ac pAb (ChIP-seq ...
• Targeted H3K9 acetylation at lncSox1 promoter by cell type-specific epigenome editing promotes intermediate progenitor proliferation in developing mouse cortex

  Godwin Sokpor, et al., bioRxiv - Neuroscience 2024

  Quote: ... BrdU rat pAb (1:100; Abcam), BrdU mouse mAb (1:40 ...
• Brown adipose tissue thermogenesis rhythms are driven by the SCN independent of adipocyte clocks

  Georgios K. Paschos, et al., bioRxiv - Physiology 2024

  Quote: ... UCP1 (Abcam; ab10983 at 1:2000), Vinculin-HRP (Cell Signaling ...
• DNA replication fork speed Acts as an Engine in Cortical Neurogenesis

  Jianhong Wang, et al., bioRxiv - Neuroscience 2024

  Quote: ... Rabbit Polyclonal anti-Pericentrin(1:250, Abcam, ab4448); Rat monoclonal anti-Neural Cell Adhesion Molecule L1(L1CAM ...
• DNA replication fork speed Acts as an Engine in Cortical Neurogenesis

  Jianhong Wang, et al., bioRxiv - Neuroscience 2024

  Quote: ... PCNT(1:2000, Abcam, ab220784), NINEIN(1:1000 ...
• DNA replication fork speed Acts as an Engine in Cortical Neurogenesis

  Jianhong Wang, et al., bioRxiv - Neuroscience 2024

  Quote: ... and Rat monoclonal anti-BrdU (1:100, Abcam, ab6326). Incubate Alexa Fluor 488 AffiniPure Donkey Anti-Mouse (1:500 ...
• Generation of humanized mouse models to support therapeutic development for SYNGAP1 and STXBP1 disorders

  Alex J. Felix, et al., bioRxiv - Neuroscience 2024

  Quote: ... and mouse anti-ATP5F1 (Abcam #ab117991, 1:1000 dilution) were used ...
• A fluorophore–ligand conjugate for the rapid and reversible staining of native AMPA receptors in living neurons

  Kyohei Soga, et al., bioRxiv - Neuroscience 2024

  Quote: ... Following primary antibodies were used: mouse anti-PSD95 (abcam, ab2723, 1:1,000) or rabbit anti-MAP2 (Millipore ...
• A fluorophore–ligand conjugate for the rapid and reversible staining of native AMPA receptors in living neurons

  Kyohei Soga, et al., bioRxiv - Neuroscience 2024

  Quote: ... rabbit anti-GluA2 antibody (abcam, ab206293, 1:3,000), and rabbit βIII tubulin antibody (abcam ...
• A fluorophore–ligand conjugate for the rapid and reversible staining of native AMPA receptors in living neurons

  Kyohei Soga, et al., bioRxiv - Neuroscience 2024

  Quote: ... goat anti-rabbit Alexa Fluor 405 conjugated (abcam, ab175652, 1:1,000) and anti-rabbit Alexa Fluor Plus 488 conjugated (Invitrogen ...
• A fluorophore–ligand conjugate for the rapid and reversible staining of native AMPA receptors in living neurons

  Kyohei Soga, et al., bioRxiv - Neuroscience 2024

  Quote: ... Samples were detected by rabbit anti-GluA1 antibody (abcam, ab19491, 1:3,000), rabbit anti-GluA2 antibody (abcam ...
• A fluorophore–ligand conjugate for the rapid and reversible staining of native AMPA receptors in living neurons

  Kyohei Soga, et al., bioRxiv - Neuroscience 2024

  Quote: ... and rabbit βIII tubulin antibody (abcam, ab18207, 1:3,000). The membrane is washed three times with TBS-T and then treated with HRP-conjugated secondary antibody (MBL ...
• Effects of HA1 (a Probucol Analogue) and ApoC3 siRNA on Lipoprotein-Amyloid Metabolism, Neurovascular Integrity and Cognitive Function in db/db Mice

  Arazu Sharif, et al., bioRxiv - Neuroscience 2024

  Quote: ... or polyclonal rabbit anti-mouse ApoB (1:200, Abcam) in blocking solution ...
• Nanotextured steel surface exhibits antifungal activity

  Anuja Tripathi, Julie A. Champion, bioRxiv - Molecular Biology 2024

  Quote: ... 3 µl of 1 mM DCFDA dye in 1X buffer and 5 µl of 10 µM DiOC2 was added for ROS evaluation (ab113851, Abcam) and membrane depolarization (B34950 ...
• Characterization of direct Purkinje cell outputs to the brainstem

  Christopher H. Chen, et al., bioRxiv - Neuroscience 2024

  Quote: ... followed by an Alexa 647 secondary antibody (1:1000 A32733 or ab150079, Thermofisher or Abcam respectively) and streptavidin-Alexa 594 conjugate (1:1000 ...
• The intersection of inflammation and DNA damage as a novel axis underlying the pathogenesis of autism spectrum disorders

  Megha Jhanji, et al., bioRxiv - Neuroscience 2024

  Quote: ... TyrRS (Abcam, #ab50961, 1:1000), TTL (Proteintech ...
• The intersection of inflammation and DNA damage as a novel axis underlying the pathogenesis of autism spectrum disorders

  Megha Jhanji, et al., bioRxiv - Neuroscience 2024

  Quote: ... TyrRS (Abcam, #ab50961, 1:50) diluted in 2% goat serum and 0.2% Triton X-100 (Lizarraga et al. ...
• The intersection of inflammation and DNA damage as a novel axis underlying the pathogenesis of autism spectrum disorders

  Megha Jhanji, et al., bioRxiv - Neuroscience 2024

  Quote: ... primary antibodies against either MAP2 (Abcam #36592 1:1250), NF-κB (Cell Signaling Technology ...
• Identification of Ephrin type-B receptor 4 as a critical mediator of tissue fibrosis

  Brian Wu, et al., bioRxiv - Pathology 2024

  Quote: ... phospho-SMAD 2/3 (Abcam, catalog #ab272332), SMAD 2/3 (Abcam ...
• Identification of Ephrin type-B receptor 4 as a critical mediator of tissue fibrosis

  Brian Wu, et al., bioRxiv - Pathology 2024

  Quote: ... elastin (Abcam, ab213720) at 1:300 ...
• Identification of Ephrin type-B receptor 4 as a critical mediator of tissue fibrosis

  Brian Wu, et al., bioRxiv - Pathology 2024

  Quote: ... Verhoeff Van Gieson’s stain (Abcam) was performed according to manufacturer’s instructions to assess elastin in lung tissue ...
• Elevated EGR1 Binding at Enhancers in Excitatory Neurons Correlates with Neuronal Subtype-Specific Epigenetic Regulation

  Liduo Yin, et al., bioRxiv - Neuroscience 2024

  Quote: ... The nuclei/beads conjugates were resuspended in 50 μL of Antibody Buffer (Wash Buffer with 0.01% Digitonin and 2 mM EDTA) containing 2 μg of H3K27ac antibody (abcam, cat# ab4729), or H3K4me1 antibody (Active Motif ...
• A comprehensive spatiotemporal map of dystrophin isoform expression in the developing and adult human brain

  Francesco Catapano, et al., bioRxiv - Neuroscience 2024

  Quote: ... Ab154168 (abcam) was used as the antibody with a concentration of 1:1500 ...
• The Alzheimer’s Disease Risk Genes MS4A4A And MS4A6A Cooperate to Negatively Regulate Trem2 and Microglia states

  Dalya Rosner, et al., bioRxiv - Neuroscience 2024

  Quote: ... cells were stained with Nuclear Green LCS1 (Abcam # ab138904) to quantify cell number ...
• Parental kynurenine 3-monooxygenase genotype in mice directs sex-specific behavioral outcomes in offspring

  Snezana Milosavljevic, et al., bioRxiv - Neuroscience 2024

  Quote: ... then incubated with a total OXPHOS rodent western blot antibody cocktail mix (Abcam AB110413, 1:15,000) prepared in 2% non-fat dry milk in TBS containing 0.2% Tween 20 (TBST ...
• Transient receptor potential vanilloid channel 2 contributes to multi-modal endoplasmic reticulum and perinuclear space dilations that can also be observed in prion-infected mice

  Wenda Zhao, et al., bioRxiv - Neuroscience 2024

  Quote: ... dilution 1:2,000 (catalog number ab176333, Abcam), mouse monoclonal IgG1 anti-ATP1A1 antibody ...
• Transient receptor potential vanilloid channel 2 contributes to multi-modal endoplasmic reticulum and perinuclear space dilations that can also be observed in prion-infected mice

  Wenda Zhao, et al., bioRxiv - Neuroscience 2024

  Quote: ... Hematoxylin and Eosin staining kit (catalog number ab245880) was purchased from Abcam (Cambridge, UK).
• Unraveling cysteine deficiency-associated rapid weight loss

  Alan Varghese, et al., bioRxiv - Physiology 2024

  Quote: ... Aliquots of lysates were filtered through 10K Amicon filters and glutathione and CoA were measured in the flow-through using the following kits: Glutathione detection kit (Cayman 703002) and CoA detection kit (Abcam ab102504). Metabolite concentration was normalized to the protein concentration in lysates ...
• Synaptic acetylcholine induces sharp wave ripples in the basolateral amygdala through nicotinic receptors

  Joshua X. Bratsch-Prince, et al., bioRxiv - Neuroscience 2024

  Quote: ... atropine (5 uM, Abcam, mecamylamine (20 uM, Millipore Sigma), and physostigmine (1 uM ...
• Synaptogyrin-3 Prevents Cocaine Addiction and Dopamine Deficits

  Emily G. Peck, et al., bioRxiv - Neuroscience 2024

  Quote: ... the primary antibody for Syngr3 was ab106460 (Abcam, 1:10,000), and the secondary antibody was Goat-anti-Mouse (Millipore Sigma ...
• Divalent siRNA for prion disease

  Juliana E Gentile, et al., bioRxiv - Neuroscience 2024

  Quote: ... The assay uses antibodies EP1802Y (Abcam, ab52604) for capture and in-house biotinylated 8H4 (Abcam ab61409 ...
• Transcriptomic changes in retinal ganglion cell types associated with the disruption of cholinergic retinal waves

  Rachana Deven Somaiya, et al., bioRxiv - Neuroscience 2024

  Quote: ... and RBPMS+ (1:500 dilution, Abcam #ab152101) cells ...
• Anti-obesity compounds, Semaglutide and LiPR, do not change the proportion of human and mouse POMC+ neurons

  Sara K.M. Jörgensen, et al., bioRxiv - Neuroscience 2024

  Quote: ... chicken anti-MAP2 monoclonal antibody (1:500, Abcam ab92434), DAPI ready-made (Sigma-Aldrich MBD0015).
• ATF5-Dependent GDF15 Expression Mediates Anesthesia-Induced Neuroprotection Against Stroke

  Xianshu Ju, et al., bioRxiv - Neuroscience 2024

  Quote: ... HSP60 (Abcam, CAT# ab46798), HSP70 (Santa Cruz Biotechnology ...
• ATF5-Dependent GDF15 Expression Mediates Anesthesia-Induced Neuroprotection Against Stroke

  Xianshu Ju, et al., bioRxiv - Neuroscience 2024

  Quote: ... p-IRE1 (S724) (Abcam, CAT# ab124945), IRE1 (Abcam ...
• ATF5-Dependent GDF15 Expression Mediates Anesthesia-Induced Neuroprotection Against Stroke

  Xianshu Ju, et al., bioRxiv - Neuroscience 2024

  Quote: ... and ATF5 (1:500; Abcam), both diluted in the blocking solution ...
• Prefrontal gamma oscillations and fear extinction learning require early postnatal interneuron-oligodendroglia communication

  Fabrice Plaisier, et al., bioRxiv - Neuroscience 2024

  Quote: ... rat monoclonal anti-MBP (1:200; clone 26; ref. AB7349, Abcam), rabbit polyclonal anti-Olig2 (1:400 ...
• Mitochondrial cardiolipin metabolism controlled by tafazzin enables ferroptosis

  Yvonne Wohlfarter, et al., bioRxiv - Molecular Biology 2024

  Quote: ... PCBP1 (Abcam, ab74793), PCBP2 (Antikörper-online ...
• Mitochondrial cardiolipin metabolism controlled by tafazzin enables ferroptosis

  Yvonne Wohlfarter, et al., bioRxiv - Molecular Biology 2024

  Quote: ... VDAC1/3 (Abcam, ab14734), FPN (Eurogentec ...