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Citations for abcam :
751 - 800 of 871 citations for SCF Human P.pastoris since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... day 1st-stimulated CAR T cells were co-cultured with γ-irradiated target cells at a 1:3 E:T ratio in the presence of 10 μg/ml plate-bound human CD226 blocking monoclonal antibody (clone DX11, Abcam, UK). Ultra-LEAF™ purified mouse IgG1(Clone MOPC-21 ...
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bioRxiv - Molecular Biology 2021Quote: Human RIPK3 rat monoclonal 1H2 (generated in-house)(Petrie et al, 2019b) Human phospho-MLKL (Ser358) rabbit monoclonal ab 187091 (Abcam) VDAC1 rabbit polyclonal AB10527 (Millipore)
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bioRxiv - Immunology 2020Quote: ... The following fluorochrome conjugated rabbit anti-human antibodies were also used in the intracellular staining: FITC anti-GLUT3 (polyclonal) and PE anti-GLUT1 (EPR3915) from Abcam. Brilliant Stain Buffer (BD Biosciences ...
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bioRxiv - Immunology 2020Quote: ... the membranes were washed with TBST and incubated with HRP-conjugated Goat-anti-human Fc antibody (Abcam, ab99759, 1:10000) for 1 h at RT ...
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bioRxiv - Cell Biology 2021Quote: The following primary antibodies were adopted for IHC and IF on mouse and human tissues: rabbit polyclonal ACE2 (1:500 pH8, ab15348, Abcam), rabbit polyclonal Prosurfactant Protein C (1:200 pH9 ...
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bioRxiv - Immunology 2020Quote: Cytokine levels in the cellular supernatant were evaluated using the FirePlex-HT assay system with the Human Cytokines FirePlex-HT Panel 1 (ab234897; Abcam). FirePlex-HT immunoassays quantify up to 10 protein analytes per sample from low sample inputs in 384-well plate format ...
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bioRxiv - Microbiology 2020Quote: ... then incubated with 0.1μg/ml HRP-conjugated Goat Anti-Human IgG H&L or HRP-conjugated Goat Anti-Rabbit IgG H&L (abcam) in 0.5% BSA in PBST for 1h at RT ...
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bioRxiv - Immunology 2021Quote: ... the membranes were washed with TBST and incubated with HRP-conjugated Goat-anti-human Fc antibody (Abcam, ab99759, 1:10000) for 1 h at RT ...
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bioRxiv - Immunology 2021Quote: ... ABCA1 (ATP-binding cassette lipid transporter marker of multivesicular bodies; mouse anti-human ABCA1; 5 μg/ml; Abcam Cat. #ab18180) and ...
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bioRxiv - Cancer Biology 2020Quote: ... rehydrated through an alcohol gradient to water and antigen retrieval in citrate buffer pH 6.0 was used for the human nucleolin antibody at 5.0 g/mL (ab13541) (Abcam, Canbridge, MA). Endogenous peroxide activity and nonspecific binding was blocked with 3%(v/v ...
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bioRxiv - Bioengineering 2022Quote: ... and a cocktail of biotinylated detection antibody (~1 ng/ml per each protein panel) and phycoerythrin (PE, 532 nm emission) conjugated anti-human IgG antibody (10 ng/ml, Abcam) was loaded onto the barcoded array slide after removing excess serum using BSA solution ...
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bioRxiv - Bioengineering 2022Quote: ... for detection (brown signals) of collagen type I (1:100 anti-human collagen I alpha-1 1200-1450 fragment (ab233080, Abcam) compatible to pig elastin [45] ...
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bioRxiv - Cell Biology 2022Quote: ... The assays were carried out in 50 μl mTOR reaction buffer with or without 200 μM ATP and/or the recombinant human His-tagged ATF4 (Cat. ab109946, Abcam) at 30°C for 45 min ...
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bioRxiv - Genomics 2022Quote: We determined the cytomegalovirus (CMV) serostatus of AFB (n = 80) and EUB (n = 80) donors with a human anti-IgG CMV ELISA kit (Abcam) on plasma samples ...
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bioRxiv - Microbiology 2022Quote: ... tween 20 in PBS (PBST) three times followed by incubation with goat F(ab’)2 anti-human IgG (Fab’)2-horseradish peroxidase (Abcam) at a 1:10,000 dilution ...
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bioRxiv - Neuroscience 2022Quote: Human serum levels of LBP were assayed using the Human LBP ELISA kit standard sandwich enzyme-linked immunosorbent technology and following the manufacturer’s protocol (Abcam, ab213805). Briefly ...
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bioRxiv - Biochemistry 2022Quote: ... and anti-human MT1-MMP rabbit monoclonal antibody EP1264Y against the catalytic domain (Abcam, catalog no. ab51074, 1:1000 dilution) were used followed by anti-rabbit secondary antibodies (Li-COR ...
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bioRxiv - Neuroscience 2022Quote: ... The expression of ACE2 Receptor protein (Rabbit Anti-Human Polyclonal, Citrate Buffer HIER, dilution 1:2000, Abcam, Code Number: ab15348) and TMPRSS-2 protein (Rabbit Anti-Human Monoclonal ...
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bioRxiv - Bioengineering 2022Quote: ... Primary antibodies (Ab) used in this study: rabbit polyclonal Ab to human serum albumin (Abcam, catalogue no. ab2406, 1:400), goat polyclonal antibody to E-cadherin (R&D systems ...
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bioRxiv - Biochemistry 2023Quote: ... and then incubated with 2% donkey serum followed by anti-human xanthine oxidase (1:1000; ab133268, Abcam, UK; RRID: AB_11154903), for 30min at 21 °C ...
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bioRxiv - Immunology 2023Quote: ... supernatants from the cocultures were tested in a hemoglobin release assay at a dilution of 1:1600 (Human Hemoglobin ELISA kit, Abcam) according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... The human origin of all patient-derived organoid xenografts (PDOXs) was confirmed by staining with an antibody recognizing human mitochondria (ab92824, Abcam).
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bioRxiv - Immunology 2022Quote: ... Plates were then washed 3x with PBS-T and incubated with HRP-conjugated goat Anti-human IgG/M antibody (Abcam) for 1h ...
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bioRxiv - Systems Biology 2023Quote: ... PVDF membranes were blocked and stained with total OXPHOS human western blot antibody cocktail (Abcam Cat# ab110411, RRID:AB_2756818, 1:500). Blots were visualized using an Odyssey infrared imaging system (RRID:SCR_022510 ...
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bioRxiv - Cancer Biology 2023Quote: ... Slides were then incubated in a humidified chamber overnight at 4°C with primary antibodies (1:50 dilution): rabbit anti-human CD20 (ab78237, Abcam), rat anti-mouse Fibroblast Marker ER-TR7 (sc73355 ...
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bioRxiv - Cancer Biology 2023Quote: ... Labelling of c-Myc positive cells was performed with human c-Myc antibody (Abcam rabbit ab#32072 dilution 1:200) and anti-rabbit secondary antibody (Thermofisher anti-rabbit IgG AF633) ...
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bioRxiv - Neuroscience 2023Quote: ... Human temporal cortex samples were lysed in CB Cell Lysis Buffer from the Cathepsin B Activity Assay Kit (Abcam, ab65300). Mouse cortex samples were lysed in RIPA buffer (150 mm sodium chloride ...
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bioRxiv - Synthetic Biology 2023Quote: The serum concentration of His-tagged PRL N59D was determined using a quantitative ELISA as follows: 50ul of 0.1ug/mL anti-human PRL antibodies (Abcam; ab244037) were diluted in PBS and used to coat MaxiSorp 96-well ELISA plates for 1hr at room temperature ...
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bioRxiv - Cell Biology 2024Quote: ... and stained with anti-human type II or I collagen antibody (1:1,000, clone II-4C11, ICN Biomedicals, Germany; or Abcam, Germany), followed by biotinylated goat anti-mouse antibody (1:500 ...
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bioRxiv - Immunology 2024Quote: ... Primary anti-human TSHR antibody was used for 60 minutes at room temperature at 1:1500 (abcam, ab218108, Cambridge, UK). Envision labeled polymer (Dako ...
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bioRxiv - Immunology 2024Quote: ... Primary anti-human TSHR antibody was used for 60 minutes at room temperature at 1:500 (abcam, ab218108, Cambridge, UK). The infiltration of human T lymphocytes into the neoplastic tumors was assessed using anti-human CD3 at 1:500 (abcam ...
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bioRxiv - Immunology 2024Quote: ... Primary anti-human TSHR antibody was used for 60 minutes at room temperature at 1:1500 (abcam, ab218108, Cambridge, UK). Slides were subsequently prepared as described in the immunohistochemistry section.
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bioRxiv - Molecular Biology 2024Quote: ... Samples were centrifuged at 1,000 g for 30 minutes before proceeding to ELISA (Human FAS Ligand ELISA Kit - CD95L –Abcam-ab100515).
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bioRxiv - Cell Biology 2024Quote: ... Membranes were probed with the following primary antibodies: rabbit polyclonal anti-calreticulin (1:1000) (ab19261, abcam; to detect human CRT); chicken polyclonal anti-calreticulin (1:1000 ...
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bioRxiv - Bioengineering 2024Quote: ... Samples were fixed at day 7 of culture and stained with CD31 human antibody (Abcam; ab134168; AF488-conjugated; 1:100) and imaged with an Inverted confocal microscope ...
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bioRxiv - Biochemistry 2024Quote: ... Wells were then washed with TBST (0.1% tween 20) and then incubated with an anti-human IgG antibody conjugated to HRP (Abcam, ab102420) for one hour at 37°C ...
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bioRxiv - Microbiology 2024Quote: To treat cells with rPAI-1 we used recombinant active human PAI-1 (carrying stabilizing mutations N150K, K154T, Q319L and M354I, Abcam) in experiments on human airway epithelial cultures and in in-vitro complex formation assays ...
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bioRxiv - Neuroscience 2023Quote: ... the membranes were washed three times with TBST and incubated with HRP-conjugated goat anti-human IgG (Fab’)2 (Abcam) or HRP-conjugated anti-rabbit IgG (Cell Signaling ...
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bioRxiv - Neuroscience 2021Quote: ... Microglia were probed with a 24h incubation at 4°C with either a rabbit antihuman IBA1 antibody diluted in blocking solution (1:200, 019-19741, Wako chemicals, USA) or a goat anti-human IBA1 antibody (1:200, ab5076, Abcam, UK). We used additional microglial markers including mouse anti-MHC-II/HLA-DP/DQ/DR (1:250 ...
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bioRxiv - Immunology 2020Quote: ... For selected experiments T cells were cultured in serum-depleted medium with or without native human C1q protein (100ug/ml, Abcam, ab96363).
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bioRxiv - Cancer Biology 2019Quote: ... surface expression of iNKG2D was correlated with GFP expression using a MicAbody and detecting with PE-anti-human kappa chain (Abcam #ab79113) or by directly conjugating the Rituximab-MicAbody to Alexa Fluor 647 (Alexa Fluor Protein Labeling Kit #A20173 ...
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bioRxiv - Microbiology 2020Quote: ... Infection was performed in the absence or in the presence of different amount of EVs or human recombinant ACE2 (Abcam, 151852). Cells were then spinoculated at 1,200xg for 1h 30 min at 25°C ...
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bioRxiv - Neuroscience 2020Quote: ... The MJFF rabbit monoclonal Rab12 pSer105 (equivalent phosphorylation site to human pSer106) antibody was described previously [21] and is available from Abcam (ab256487). Recombinant anti-LRRK2 pSer1292 MJFR-19-7-8 (ab203181) ...
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bioRxiv - Microbiology 2020Quote: ... peroxidase-labeled (Cat. 04-10-06, KPL, USA) or goat anti-human IgA alpha chain (alkaline phosphatase) (Cat. Ab97212, Abcam, UK), diluted 1:1000 in blocking solution ...
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bioRxiv - Cell Biology 2021Quote: The following primary antibodies were used: C-terminus (aa 450-499) of human Cdc20 (C-term Ab, 1:500, Abcam, ab26483), N-terminus (aa 1-175 ...
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bioRxiv - Cell Biology 2021Quote: ... cut based on the molecular weights indicated by the prestained marker and then incubated overnight on a shaker at 4 oC with rabbit anti-human STEAP3 (1:1000) (abcam, 151566), mouse anti-human p53 (1:1000 ...
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bioRxiv - Genomics 2022Quote: ... Immunostaining was performed using a rabbit polyclonal to human SC lateral element protein SCP3 (1:500 dilution; Cat# ab15093, Abcam; RRID:AB_301639) and a Cy3-labeled donkey anti-rabbit IgG (H+L ...
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bioRxiv - Cell Biology 2020Quote: Supernatant was harvested from HeLa tBID2A cells as described above and used to probe a Human Growth Factor Antibody Array (Abcam, ab134002) according to the manufacturer’s instructions.
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bioRxiv - Biochemistry 2020Quote: ... the protein expression was verified by transient transfection and western blot detection with goat anti-human polyclonal horse radish peroxidase (HRP) conjugated antibody (Abcam ab98567). A stable Drosophila S2 cell line for SALM3 LRR expression was then generated for the protein purification ...
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bioRxiv - Neuroscience 2021Quote: ... NT-3 was measured by standard ELISA according to manufacturer’s instructions (ab100615 – Neurotrophin 3 human ELISA Kit, abcam, Cambridge, United Kingdom).