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Citations for abcam :
751 - 800 of 10000+ citations for Cytosolic arginine sensor for mTORC1 subunit 2 CASTOR2 Antibody HRP since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2021Quote: ... the membranes were blocked with 5% milk (w/v) in TBST (TBS, 0.1% Tween 20) and were probed with horseradish peroxidase (HRP) conjugated anti-His antibody (Abcam, catalog # ab1187) or the C ...
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bioRxiv - Microbiology 2021Quote: ... horseradish peroxidase (HRP)-conjugated goat anti-mouse antibodies against the light chain (Fab) and heavy chain (Fc) (Abcam,plc, Cambridge, MA), and HRP-conjugated goat anti-rabbit antibody (Abcam ...
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bioRxiv - Microbiology 2020Quote: ... 50 μg of recombinant Rv3351c protein and anti-6xHIS antibody conjugated to horseradish peroxidase (HRP, 1:500; Abcam Inc., Cambridge, MA) were mixed together in PBS containing 0.5% BSA ...
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bioRxiv - Immunology 2022Quote: ... washed twice in TBS-T and incubated in the presence of a goat HRP conjugated anti-rabbit secondary IgG antibody (1:10000; Abcam 6721). Membranes were revealed using the Amersham ECL prime reagent (GE healthcare ...
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bioRxiv - Biochemistry 2020Quote: ... the protein expression was verified by transient transfection and western blot detection with goat anti-human polyclonal horse radish peroxidase (HRP) conjugated antibody (Abcam ab98567). A stable Drosophila S2 cell line for SALM3 LRR expression was then generated for the protein purification ...
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bioRxiv - Neuroscience 2020Quote: ... the blot was incubated with 1:1000 diluted buffer of secondary rabbit anti-mouse HRP conjugated antibody (Cat # 6728, Abcam, UK) in 1X PBS for 1 h at room temperature ...
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bioRxiv - Developmental Biology 2020Quote: ... SPE-18 protein was detected by a 1:5000 dilution of rabbit anti-SPE-18 polyclonal antibody (YenZym) and HRP conjugated goat-anti rabbit IgG (Abcam #ab6721). MSP was detected by a 1:10000 dilution of mouse anti-MSP monoclonal antibody 4A5 (Kosinski et al. ...
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bioRxiv - Biochemistry 2022Quote: ... Detection of CMY-2 was performed by adding 500 ng/well of pAbs themselves followed by the addition of a 1/2000 diluted goat anti-rabbit antibody conjugated to HRP (Abcam, UK) or by adding 200 ng/well of monovalent or bivalent cAbCMY-2 (254 ...
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bioRxiv - Immunology 2022Quote: ... The slides were incubated with primary rabbit anti-SARS nucleocapsid protein antibody(NB100-56576, Novus, USA) overnight at 4°C and then with goat anti-rabbit IgG HRP (Abcam, UK) for 1 hr ...
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bioRxiv - Plant Biology 2024Quote: ... blots were stripped and re-probed with anti-HSC70 monoclonal antibody (Enzo #ADI-SPA-818-D) at 1/5000 dilution followed by Rabbit Anti-Mouse IgG H&L (HRP) secondary antibody (AbCam ab6728) at 1/10000 dilution ...
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bioRxiv - Plant Biology 2024Quote: ... Both were used at 1/5000 dilution and followed by Goat Anti-Rabbit IgG H&L (HRP) secondary antibody (AbCam ab6721) used at 1/10000 dilution ...
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bioRxiv - Immunology 2023Quote: ... was incubated with the transferred proteins for 2 h and the binding was detected after incubation for 1 h at RT with HRP-conjugated anti-HA antibody diluted 1:5000 (Abcam, Ab1190). The binding of the conjugate was detected with ECL Western Blotting Substrate (Thermo Fisher Scientific ...
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bioRxiv - Zoology 2023Quote: ... The signal from the secondary antibody was amplified using an HRP-conjugated streptavidin (1:10,000 in PBS; ab7403, Abcam, Cambridge, UK). Colour development was achieved with TMB substrate (1 mg L-1 in 0.05M phosphate-citrate buffer ...
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bioRxiv - Cancer Biology 2023Quote: ... Sections were washed with PBS and incubated in the blocking solution containing anti-goat IgG H&L HRP-conjugated secondary antibody (1:1000) (Abcam, ab6885) for 1hr ...
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bioRxiv - Microbiology 2022Quote: ... The plates were washed three times with PBS-T and 100 uL anti-hamster IgG horseradish peroxidase antibody (HRP, abcam, #ab6892) diluted 1:10,000 in PBS-T containing 1% milk powder was added to all wells ...
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bioRxiv - Cancer Biology 2023Quote: ... the slides were incubated with the corresponding HRPconjugated secondary antibody for one hour at room temperature (goat anti-rabbit IgG H&L HRP, Abcam #ab6721). The slides were then washed in PBS 3 times then developed using the DAB substrate kit (Abcam #ab64238) ...
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bioRxiv - Microbiology 2024Quote: ... The membrane was washed 3 times in TBST and incubated with the secondary antibody (donkey anti-rabbit IgG HRP conjugate, AbCam 16284) diluted 1/5000 in TBST for 2 h at room temperature ...
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bioRxiv - Immunology 2020Quote: ... Reacted mAbs were detected using ALP-conjugated Goat F(ab’)2 Anti-Human (IgG (Fab’)2) secondary antibody (Abcam, ab98532, 1:2000) for 30 min at RT ...
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bioRxiv - Biochemistry 2021Quote: ... for 10 min and then incubated with the following secondary antibody for 2 hrs: goat anti-mouse IRDYe® 680 conjugated antibody (1:10,000; abcam; ab216776). The membrane was washed again with PBST three times for 10 min and imaged on the Odyssey Li-Cor.
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bioRxiv - Microbiology 2021Quote: ... Gels were either stained with Coomassie brilliant blue or subjected to Western blot using appropriate antibodies: rabbit polyclonal anti-SARS-CoV-2 S antibody (Abcam ab272504) or rabbit polyclonal anti-SARS-CoV/CoV2 M antibody (Novus Biologicals NB100-56569).
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bioRxiv - Neuroscience 2021Quote: ... Antibodies used were α-syn aggregate-specific antibody MJF-14 (rabbit monoclonal MJFR-14-6-4-2, #ab209538, Abcam, 1:25,000) and mouse monoclonal pS129-α-syn (11A5 ...
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bioRxiv - Genomics 2021Quote: ... were used to capture 400 μL of SUMO-2/3 antibody (8A2, acquired from Abcam, ab81371; ∼5-10 μg/μL antibody). All washing and handling steps were followed by centrifugation of the beads at 500g for 3 min in a swing-out centrifuge with delayed deceleration and careful aspiration of buffers ...
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bioRxiv - Physiology 2021Quote: ... Baltimore PKD Center), anti-PC-2 monoclonal antibody (3374 CT-1 414, Baltimore PKD Center) and anti-CD31 primary monoclonal antibody (Abcam 7388) at 4°C ...
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bioRxiv - Bioengineering 2024Quote: ... cells were incubated with rabbit Human Protein Atlas antibodies (HPA072117 for NANOG, HPA058267 for POU5F1) at 2 µg/mL and mouse anti-tubulin antibodies (Abcam ab7291) at 1 µg/mL ...
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bioRxiv - Neuroscience 2023Quote: ... Free floating sections were incubated in 1% hydrogen peroxide for 15 minutes then incubated with anti-ATP synthase subunit C (Abcam, ab181243; 1:1000), anti-GFAP (Dako ...
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bioRxiv - Cell Biology 2020Quote: ... Western blotting was carried out as described (Hinshaw et al., 2019) with the following antibodies: anti-FLAG-HRP (Abcam 49763 from ascites), anti-PGK1 (Invitrogen 459250) ...
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bioRxiv - Microbiology 2021Quote: ... Blots were washed again in 3X TBS-T then incubated in blocking buffer containing a 1:5000 dilution of rabbit Anti-DDDDK HRP conjugated antibody (Abcam Cat #ab2493). Blots were washed three times with TBST and once with TBS ...
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Role of autophagy in sepsis-induced skeletal muscle dysfunction, whole-body metabolism, and survivalbioRxiv - Cell Biology 2021Quote: ... Membranes were blocked in PBS + 1% Tween® 20 + 5% BSA for 1 h at room temperature then incubated overnight with specific primary antibodies at 4°C.Membranes were washed in PBST (3×5 min) then incubated with HRP-conjugated goat anti-rabbit (ab6721) or rabbit anti-mouse (ab6728) secondary antibodies (Abcam, Cambridge, MA) for 1 h at room temperature ...
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bioRxiv - Microbiology 2020Quote: ... the membrane was washed three times with Tris-buffered saline containing 0.01% Tween 20 and incubated for one hour with secondary mouse HRP-conjugated antibody (Abcam Inc., Cambridge, MA). All incubations and wash steps were performed at room temperature unless otherwise stated ...
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bioRxiv - Molecular Biology 2020Quote: ... membranes were incubated with secondary antibody for 1 hr at room temperature: rat-HRP 1:10,000 dilution (abcam ab205720 Lot# GR32115155-1), rabbit-HRP 1:10,000 dilution (Santacruz sc-2004 Lot#B2216) ...
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bioRxiv - Plant Biology 2020Quote: ... Membranes were washed 3 times for 15 mins in 1X Tris-buffered saline (TBS) with 0.1% Tween20 (TBST) and incubated with HRP-conjugated goat anti-mouse antibody (abcam, catalog number ab6789). All antibodies were used at a concentration on 1:5000 ...
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bioRxiv - Synthetic Biology 2022Quote: ... 0.1% Tween 20) and subsequently probed for 1 h with one of the following: horseradish peroxidase (HRP)-conjugated anti-DDDDK antibody (Abcam, cat # ab49763) that recognized the FLAG epitope tag ...
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bioRxiv - Immunology 2024Quote: ... anti-beta Actin HRP [AC-15] (#ab49900, diluted at 1:10000), anti-IL-1 beta (#ab2105, diluted at 1:1000) antibodies from Abcam (Cambridge, UK), anti-AMPKα2 (#AF2850 ...
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bioRxiv - Bioengineering 2023Quote: ... which was prepared by 5% milk in TBS-T containing 1:10,000 horseradish peroxidase (HRP)-conjugated goat anti-mouse secondary antibodies (Abcam ab6789-1 MG). The secondary antibody incubation was applied for 1 hour at room temperature ...
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Mucociliary Clearance Augmenting Drugs Block SARS-Cov-2 Replication in Human Airway Epithelial CellsbioRxiv - Microbiology 2023Quote: ... sections corresponding to tubulin staining were incubated with a goat anti-mouse IgG secondary antibody conjugated with HRP (Abcam ab6789, 1:500), while sections of caspase and protein S staining were incubated with goat anti-rabbit IgG secondary antibody conjugated with HRP (Abcam ab6721 ...
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bioRxiv - Synthetic Biology 2023Quote: ... The membrane was washed with TBS-T and incubated for 1 hour at room temperature with a 1:10,000 dilution of HRP-conjugated goat anti-mouse secondary antibodies (Abcam ab6789-1 MG) in 5% milk-TBS-T ...
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bioRxiv - Cancer Biology 2023Quote: ... The membranes were washed with TBST and incubated with the HRP-conjugated secondary antibodies (Goat pAb to Rabbit and Mouse IgG, Abcam, ab205718, ab205719) for 1 h at rt ...
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bioRxiv - Biochemistry 2023Quote: The following secondary antibodies have been used for Western blotting: Goat Anti-Mouse IgG H&L (HRP) preadsorbed (Abcam, #ab7068; 1:10000), Recombinant Protein G (HRP ...
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bioRxiv - Immunology 2023Quote: ... The plates were washed three times with PBST and then incubated with anti-mouse IgG or IgA secondary antibody conjugated to horseradish peroxidase (HRP) (Abcam, Cambridge, UK) in PBST for 1 h at room temperature ...
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bioRxiv - Immunology 2024Quote: ... The membranes were washed and incubated with the respective HRP-coupled secondary antibodies for one hour at room temperature: goat anti-rabbit IgG H&L (#ab205718, 1:5000; abcam; RRID: AB_2819160), Goat Anti-Mouse IgG (H + L ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... or rabbit anti-human GPR109A (also called hydroxycarboxylic acid receptor 2) antibody (Abcam, USA) at 1:500 ...
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bioRxiv - Plant Biology 2020Quote: ... The DNA-free RNAs were then inoculated with 2 μg anti-BrdU antibody (abcam) at room temperature for 3O min and precipitated by Dynabeads (Thermo) ...
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bioRxiv - Developmental Biology 2020Quote: ... or with the anti-tyrosinated tubulin antibody YL1/2 (1:50, rat; Abcam, #6160). Primary antibodies were detected ...
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bioRxiv - Genomics 2021Quote: ... Immunoprecipitations were carried out with 10 μg of SUMO-2/3 antibody (ab3742, Abcam) and 420 μl of Dynabeads Protein A (10001D ...
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bioRxiv - Genetics 2020Quote: ... The worms’ DNA was incubated with specific anti-8-Hydroxy-2’-deoxyguanosine antibody (Abcam) in immunoprecipitation buffer (2.7 mM KCl ...
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bioRxiv - Cell Biology 2021Quote: ... GAPDH was probed with a rabbit polyclonal antibody (Abcam, ab9485, GR3212164-2, 1:2500). hKid was probed with a monoclonal rabbit antibody (Abcam ...
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bioRxiv - Cell Biology 2022Quote: ... anti-SARS-CoV-2 nucleocapsid protein rabbit polyclonal antibody (dilution 1:1000; #ab273167, Abcam); Alexa Fluor 488 goat anti-rabbit IgG (H+L ...
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bioRxiv - Microbiology 2023Quote: ... followed by incubation with anti-SARS-CoV-2 S antibody (1:1,000) (ab272504, Abcam) for 1 h ...
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bioRxiv - Molecular Biology 2024Quote: ... 10-25 μg of chromatin was incubated with 1-2 μg of antibodies (Abcam: 9E10 ...
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bioRxiv - Neuroscience 2023Quote: ... and chicken anti-microtubule-associated protein 2 (MAP2) antibody (Abcam, Cat# ab92434, 1:70)] ...