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Citations for abcam :
751 - 800 of 2760 citations for 5 2 3 Difluorophenyl 5 oxovaleric acid since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: ... Sections were then washed 8×5 min in PBS before being mounted onto glass slides and cover-slipped with fluoroshield mounting medium with DAPI (AbCam). Dual staining was performed with sequential primary-secondary incubations ...
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bioRxiv - Microbiology 2020Quote: ... Membranes containing proteins were blocked in 5% BSA and incubated with rabbit anti-HMGB1 polyclonal antibody (1:1,000, Abcam, U.S.A), followed by incubation with horseradish peroxidase-linked secondary antibodies (1:5,000 ...
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bioRxiv - Neuroscience 2020Quote: ... Slides were blocked in 5% foetal bovine serum (FBS) for 30 min and incubated overnight in rabbit anti-PSD-95 (1:100; Abcam, Cambridge ...
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A novel neural stem cell-derived immunocompetent mouse model of glioblastoma for preclinical studiesbioRxiv - Cancer Biology 2020Quote: Formalin fixed paraffin-embedded sections (5 μm) were stained as described previously34 using the following primary antibodies: rabbit anti-Ki67 (ab15580, Abcam), rabbit anti-OLIG2 (ab109186 ...
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bioRxiv - Cell Biology 2021Quote: ... Membranes incubated with the primary antibodies against claudin-5 (1:1000, Zymed 1600-34) or GAPDH (1:400, ab181602, ABCAM)) and the appropriate secondary antibodies.
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bioRxiv - Microbiology 2021Quote: Clonal lines were screened for knockout by labeling with primary mouse monoclonal antibodies to CD59 (clone MEM-43/5; Abcam) or CD46 (clone C-10 ...
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bioRxiv - Microbiology 2021Quote: ... Membranes were blocked with 5% BSA in TBST then probed with primary antibodies for HMGCR (Ms mAb, 1:000, abcam), MVD (1:1000 ...
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bioRxiv - Plant Biology 2020Quote: ... The membrane was blocked with 5% non-fat skim milk and immunoblots were performed with indicated primary antibodies [anti-SUMO1 (Abcam), anti-Actin C3 (Abiocode) ...
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bioRxiv - Cell Biology 2021Quote: ... The membrane was washed and then incubated at RT for 1 h with horseradish-peroxidase (HRP)-conjugated goat anti-mouse (IgG) polyclonal antibody as the secondary antibody (1:5 000; Abcam). After three washes ...
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bioRxiv - Cell Biology 2020Quote: ... and heated to 95 °C for 5 min (no heating of the samples used for the total oxphos (abcam, ab110413) blots) ...
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bioRxiv - Neuroscience 2020Quote: ... They were then blocked with rotation at room temperature for an hour in blocking buffer (5% normal goat serum (Abcam), 0.2% TritonX-100 PBS) ...
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bioRxiv - Cell Biology 2022Quote: ... paraffin slices were blocked for 1 hour at 37°C with 5% BSA before being treated overnight at 4°C with various primary antibodies as follows: anti-GFAP (Abcam), anti-CD68 (Abcam) ...
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bioRxiv - Physiology 2022Quote: ... blocked in 5% goat serum for 1 hour and stained using myelin protein zero (MPZ) (1 in 50 in 5% goat serum in PBS containing 1% triton) (EPR20383, Abcam) overnight ...
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bioRxiv - Cancer Biology 2022Quote: ... Coverslips were then washed with 1xPBS 3x for 5 minutes before incubation in 50 µl spots of secondary antibodies (goat anti-mouse AF647: Abcam ab150115 ...
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bioRxiv - Molecular Biology 2021Quote: ... the cells were permeabilized with 0.1% Triton X 100 in 1X PBS for 5 min and blocked with Protein Block (Abcam, USA) for 20 min at room temperature ...
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bioRxiv - Immunology 2021Quote: ... Samples were blocked with 5% normal goat serum for 1 hr and incubated in anti-TRPM4 (Abcam, catalog no. ab106200) or normal mouse IgG (Santa Cruz Biotechnology ...
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bioRxiv - Immunology 2021Quote: ... 1×106 cells were blocked with DPBS with 5% of normal mouse serum (cat. n° ab7486, Abcam, Cambridge, MA, USA) for 30 min at 37°C ...
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bioRxiv - Cell Biology 2021Quote: ... 0.25 x 106 cells were seeded into 10 mL cell culture dishes then treated with 5 ng/mL TGFbeta (Abcam) on the next day ...
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bioRxiv - Microbiology 2021Quote: ... Membranes were blocked in TBS-T with 5% skim milk powder at 4°C overnight and incubated in 1:8000 diluted anti-GFP antibody (Ab290; Abcam) in TBS-T with 5% skim milk powder for one hour at room temperature ...
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bioRxiv - Systems Biology 2021Quote: ... All primary and secondary antibodies were diluted 1:400 in PBS containing either 2.25% bovine serum albumin [Millipore Sigma #A2058] or 5% normal goat serum (Abcam #ab7481) for blocking and 0.15% Triton X-100 (Sigma Aldritch #T8787 ...
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bioRxiv - Immunology 2022Quote: ... were washed with lysis buffer (containing protein inhibitors) and incubated with 5 µg of 4C4 antibody or IgG1k as a control isotype (ab18443 Abcam) at 4°C overnight under rotation ...
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bioRxiv - Synthetic Biology 2020Quote: ... 5 μL FLAG tag antibody (Anti-DDDDK-PE, Abcam ab72469, RRID: AB_1268475, or Anti-DDDDK-APC, Abcam ab72569, RRID: AB_1310127) was added at a concentration of 0.5 μg per sample and cells incubated at 4°C for 30 min ...
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bioRxiv - Pathology 2021Quote: ... The antigen was retrieved by incubating tissue sections for 5-7 minutes in Proteinase K antigen retrieval solution (Abcam, ab64220). Incubation with primary monoclonal antibodies against HCN1 (Novus Biologicals ...
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bioRxiv - Immunology 2020Quote: ... Sections were blocked with protein block for 5 min and incubated overnight at 4°C with either rabbit polyclonal anti-CD3 antibody (1:50 dilution, ab5690, Abcam) or rabbit monoclonal negative control anti-IgG (1:50 dilution ...
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bioRxiv - Immunology 2020Quote: ... serum was diluted 1:5 in diluent buffer and run in duplicate using Mouse LBP ELISA Kit (Abcam, Cambridge, MA) according to manufacturer’s instructions.
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bioRxiv - Physiology 2021Quote: ... Membranes were blocked in TBS-Tween 20 (0.1%) (TBST) with 5% BSA and then incubated overnight with primary antibodies for OXPHOS (Abcam MS604), P-RPS6 Ser235/236 (Cell Signaling ...
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bioRxiv - Cancer Biology 2021Quote: ... tissue sections were blocked with 5% mouse serum PBS1X for 1h30 and incubated with mouse anti-tryptase antibody (1:300, ab2378, RRID:AB_303023, ABcam, Cambridge, UK) for 1h at RT ...
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bioRxiv - Immunology 2021Quote: ... ABCA1 (ATP-binding cassette lipid transporter marker of multivesicular bodies; mouse anti-human ABCA1; 5 μg/ml; Abcam Cat. #ab18180) and ...
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bioRxiv - Cell Biology 2020Quote: Exosomes (25 μg prot) from A-375 cells were incubated or not with 5 µl anti-CD63 antibodies (Abcam, ab8219) for 2 h at room temperature ...
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bioRxiv - Genomics 2021Quote: ... 500 µL of supernatant fluid was added to 25 µL of pre-incubated protein G magnetic beads (ChIP-Adembeads ChIP-Adem-Kit, Ademtech SA) with 5 µg of anti-V5 rabbit polyclonal antibody (ab9116, Abcam) in IP buffer (50 mM HEPES-KOH pH 7.5 ...
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bioRxiv - Neuroscience 2022Quote: Immunohistochemistry was performed for neuroblast detection and analysis of hippocampal neurogenesis using the following primary antibodies diluted in PBS containing 5% goat serum and 0.1% Triton-X-100: anti-doublecortin (DCX, rabbit: 1:5000, Abcam, #ab18723), anti-bromodeoxyuridine (BrdU ...
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bioRxiv - Cancer Biology 2022Quote: ... The following antibodies were used with a total of 5 µg of each primary antibody used per immunoprecipitation: Rabbit anti-LSD1 (ab17721, Abcam), Rabbit ani-MYC (13987 ...
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bioRxiv - Cell Biology 2022Quote: ... Anti-Cullin 5(ab184177), anti-Elongin B antibody (ab154854, 1:2000) and anti-SOCS2 antibody (ab109245, 1:1000) were purchased from Abcam. Antibody binding was visualized with peroxidase-conjugated sheep anti-rabbit immunoglobulin (Southern Biotech ...
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Evaluation of the DBA/2J mouse as a potential background strain for genetic models of cardiomyopathybioRxiv - Physiology 2022Quote: ... blocked in 5% BSA-PBS at room temperature and incubated with the anti-syntrophin primary antibody (1:2000; #11425; Abcam) overnight at 4°C ...
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bioRxiv - Microbiology 2022Quote: ... Tween-20 and 5% (w/v) milk powder containing 1:5,000 horseradish peroxidase-conjugated goat anti-mouse IgG secondary antibody (AbCam ab205719) and incubated on a shaking platform for 1 hr at room temperature ...
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bioRxiv - Neuroscience 2022Quote: ... They were then blocked with rotation at room temperature for an hour in blocking buffer (5% normal goat serum (Abcam), 0.2% TritonX-100 PBS) ...
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bioRxiv - Molecular Biology 2022Quote: ... Membranes were washed 5 times with 1× PBST and incubated with goat anti-mouse horseradish peroxidase-conjugated secondary antibody (Abcam) for 30 mins then visualized using the chemiluminescence kit (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2024Quote: ... 5% CO2 for 24 h before supernatants were collected and LDH levels determined using an LDH-Cytotoxicity Assay Kit (abcam).
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bioRxiv - Pathology 2024Quote: ... paraffin-embedded 5 µm slices were stained for insulin (ab195956, 1:1500, Alexa Fluor 555-conjugated secondary antibody: ab150186, 1:500, Abcam) and glucagon (ab92517 ...
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bioRxiv - Neuroscience 2024Quote: ... The slides were then incubated for 60 minutes at room temperature in blocking buffer (PBS with 5 % goat serum [Abcam #ab7481] and 0.3 % Triton X-100 [Bio-Rad Laboratories #161-0407]) ...
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bioRxiv - Physiology 2023Quote: ... This was followed by placing in 5% normal goat serum for 1h and labelling for mCherry with rabbit anti-mCherry (1:10,000; Abcam; RRID:AB_2571870) and goat anti-rabbit 568 (1;400 ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were then incubated overnight at 4⁰C on a plate rocker in a 5% NGS/0.1% PBS-Triton solution containing rabbit anti-cFos antibody (Abcam, ab190289) at 1:500 dilution ...
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bioRxiv - Neuroscience 2024Quote: ... and blocked with 5% milk in TBS-Tween 20 for 2h and incubated with primary antibodies diluted in the 5% BSA in TBS-Tween 20 overnight at 4°C (AMPD2, Sigma HPA045760, 1:1000; IMPDH2, Abcam ab129165 ...
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bioRxiv - Neuroscience 2024Quote: ... the membrane was washed in TBS-T three times for 5 minutes each and incubated with goat anti-mouse IgG HRP (1:5000, cat# AB205719, Abcam) in TBS-T for 1 h at room temperature ...
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bioRxiv - Microbiology 2024Quote: ... Cells were then permeabilized in 1mL of 0.1% Triton X-100 in 1X PBS for 5 min at room temperature and incubated with anti-DDX5 primary antibody (rabbit, ab21696; Abcam) diluted to 1:400 in PBS 1X for 1 hour ...
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bioRxiv - Cancer Biology 2024Quote: ... The membranes were incubated with the following primary antibodies in 5% skim milk at 4 °C overnight: rabbit anti-ATF4 antibody (ab270980, 1:1000, Abcam), XBP1 antibody (ab37152 ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... The slices were blocked with 5% goat serum (Beyotime, China) for 30 min and then incubated with anti-Aβ1-42 (1:300, Abcam, UK), anti-Tau(pSer396 ...
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bioRxiv - Biochemistry 2023Quote: ... samples were washed with 5 mL of 0.1% Triton X-100/PBS and incubated with 1 μL of IRF5 antibody (Abcam, 181553) in 100 μL of 0.1% Triton X-100/PBS for 2 h at RT ...
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bioRxiv - Cancer Biology 2023Quote: ... were sonicated and incubated overnight under rotation at 4 °C with 5 ug/ml of anti-RNAPII-Ser2p (Abcam, ab193468) or anti-IgG (Cell Signaling ...
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bioRxiv - Biochemistry 2024Quote: ... Membranes blocked for 1 h at room temperature were probed overnight at 4 °C in 5% non-fat dry milk with 1:2,000 anti-CA (Abcam ab63917) or 1:5,000 anti-IN77 ...