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Citations for abcam :
7901 - 7950 of 10000+ citations for Anti INTS9 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2019Quote: ... and treated with blocking solution (TBS containing 5% normal goat serum and 0.2% Triton X-100) for 2 hr and incubated overnight at 4°C with chicken anti-GFP monoclonal antibody (1:500; abcam; ab13970). After washing three times with TBST (TBS containing 0.2% Triton X-100) ...
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bioRxiv - Genomics 2019Quote: ... KLF4 protein expression has been checked by western blot using an antibody from Santa-Cruz (#sc-20691, now discontinued) and using H3 as a loading control (anti-H3, Abcam, ab1791).
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bioRxiv - Genetics 2020Quote: ... the membranes were then probed at 4°C overnight with various primary antibodies: anti-foxa2 (Seven Hills) or LXR-α (Abcam), washed with TBST (20 mM Tris ...
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bioRxiv - Developmental Biology 2019Quote: ... After washing 3 times with TBS-T the blots were incubated with HRP-conjugated secondary antibodies (goat-anti-mouse HRP (Abcam, ab6789); goat-anti-rabbit HRP (Abcam ...
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bioRxiv - Biophysics 2019Quote: ... for 1h at (20°C) followed by an overnight incubation at (20°C) in primary Rabbit anti-porcine circovirus antibody (Cab 183908, Abcam, UK) diluted with blocking buffer ...
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bioRxiv - Molecular Biology 2019Quote: ChIP-seq experiments were performed as described previously (Ninova et al., accompanying manuscript) with the following antibodies: anti-H3K9me3 (Abcam, ab8898), anti-RNA Pol II (Abcam ...
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bioRxiv - Cell Biology 2019Quote: ... and then incubated overnight at 4°C in wash buffer containing Anti-CDKN2A/p16INK4a conjugated (Alexa Fluor 647) antibody (Abcam ab192054). Cells were then washed with PBS containing 1 µg/mL DAPI for five minutes at room temperature ...
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bioRxiv - Biochemistry 2020Quote: ... the protein expression was verified by transient transfection and western blot detection with goat anti-human polyclonal horse radish peroxidase (HRP) conjugated antibody (Abcam ab98567). A stable Drosophila S2 cell line for SALM3 LRR expression was then generated for the protein purification ...
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bioRxiv - Microbiology 2019Quote: ... A 96-well ELISA plate (Nunclon® Immobilon) was coated with 70 µL of polyclonal rabbit anti-Salmonella antibody ab35156 (Abcam) suspended in PBS (5 µg/mL ...
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bioRxiv - Biochemistry 2021Quote: ... The stability of the recombinant protein during the purification was monitored by SDS-PAGE and Western blot analysis (WB) using a rabbit anti-CSB primary antibody (ABCAM: ab96089) and a goat anti-rabbit HRP secondary antibody (ABCAM ...
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bioRxiv - Neuroscience 2020Quote: ... the blot was incubated with 1:1000 diluted buffer of secondary rabbit anti-mouse HRP conjugated antibody (Cat # 6728, Abcam, UK) in 1X PBS for 1 h at room temperature ...
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bioRxiv - Molecular Biology 2020Quote: ... proteins were blocked with 1% normal goat serum (30 min at room temperature) followed by a goat anti-mouse IgG2a antibody (Abcam, ab98694). After additional blocking with normal goat serum ...
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bioRxiv - Neuroscience 2020Quote: ... Anti-Synapsin 1 antibody (cat no: ab64581, Used at 1:1000 dilution for western blotting) was obtained from Abcam (Cambridge, UK). Glutamate (25μM) ...
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bioRxiv - Molecular Biology 2021Quote: ... The membrane was washed twice with PBST for 10 minutes and then incubated with HA-tag primary anti-rabbit antibody (Abcam ab236623) for 2 hours with shaking ...
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bioRxiv - Immunology 2021Quote: ... For detection of IgG subclasses all serum samples were diluted to 1 total IgG EU and incubated at 37 °C for 1 h prior to detection with Alkaline Phosphatase conjugated anti-mouse IgG subclass-specific secondary antibodies (Southern Biotech or Abcam) incubated for 1 h at 37 °C ...
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bioRxiv - Immunology 2020Quote: ... Sections were incubated overnight at 4°C with a rabbit polyclonal anti-mouse blocking antibody (MUC2, ab90007, Abcam Ltd; Occludin, ab168986, Abcam Ltd; caspase 3, ab44976, Abcam Ltd). The sections were washed with PBS and incubated with a secondary antibody for 30 min ...
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bioRxiv - Animal Behavior and Cognition 2019Quote: ... primary antibodies used were polyclonal rabbit α-Nowl (1:200) and FITC-conjugated polyclonal goat anti-GFP (1:200; Abcam, #ab6662). Biotinylated anti-rabbit was applied (1:200 ...
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bioRxiv - Cell Biology 2019Quote: ... Tendon stromal cells isolated from healthy volunteers and patients with tendon tears (n=3 each) were incubated with the following primary antibodies: anti-ALX (Abcam, ab26316), anti-ALOX15 (Abcam ab119774) ...
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bioRxiv - Cell Biology 2019Quote: COS7 cells expressing Sec23-GFP and VSVG-Scarlet under BFA/Noc treatment were fixed 30 min after shift to permissive temperature for immuno-EM(Beznoussenko et al., 2014) Cells were then incubated with polyclonal anti-GFP antibody (Abcam, UK) for 2 hours and subsequently with anti-rabbit Fab’ fragment nanogold conjugates (NanoProbes ...
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bioRxiv - Neuroscience 2019Quote: ... incubated in 5% BSA diluted in TBST for 1hr and then incubated with primary anti-C-terminus GluR1 antibody overnight (Abcam, ab31232). Membranes were washed in TBST (3 × 5 min) ...
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bioRxiv - Systems Biology 2019Quote: ... KO of SDCBP was confirmed by Western Blot127 with the rabbit anti-SDCBP antibody (ab133267 with lot number GR282684-7) purchased from Abcam (Cambridge).
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bioRxiv - Genomics 2021Quote: ... CHD4 complexes were solubilized and immunoaffinity purified as previously described (Cristea and Chait 2011; Kaltenbrun et al. 2013) using rabbit anti-CHD4 antibody (Abcam #ab72418) or negative control custom rabbit anti-GFP antibody (Cristea et al ...
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bioRxiv - Genomics 2021Quote: ... The cells were resuspended by gently flicking the tube in the same blocking buffer containing 1:250 dilution of rabbit anti lamin-B1 antibody (Abcam, #ab16048) and incubated overnight at 4 °C in a microfuge tube rack ...
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bioRxiv - Physiology 2021Quote: ... Cells were washed three times with cold PBS and incubated with 1% bovine serum albumin (BSA) in PBS for 10 min and then with goat anti-TRPM7 antibody (ab729; Abcam Biochemicals) for 16 h at 4°C ...
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bioRxiv - Neuroscience 2020Quote: ... The proteins of interests were detected using the following primary antibodies: anti-rabbit Catalase (1:375; catalog #Ab52477; Abcam, Cambridge, MA); anti-rabbit Glutathione Peroxidase (1:500 ...
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Molecular architecture determines brain delivery of a transferrin-receptor targeted lysosomal enzymebioRxiv - Neuroscience 2021Quote: ... Sections were then incubated in primary antibody (Jackson ImmunoResearch 109-605-003: Goat anti-huIgG conjugated to Alexa Fluor 647, 1:250, Abcam GL2A7 ...
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Satb2 acts as a gatekeeper for major developmental transitions during early vertebrate embryogenesisbioRxiv - Developmental Biology 2020Quote: ... Non-specific sites on the membrane were blocked using 3% BSA and further incubated with anti-SATB2 antibody (1:1000 abcam ab34735) in 0.3% BSA overnight at 4°C with constant rocking ...
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bioRxiv - Neuroscience 2021Quote: ... constant shaking) with the following primary antibodies: sheep anti-phospo-S6 ribosomal protein (pS6; 1:250, ab65748, Abcam, Cambridge, Massachusetts, USA); mouse anti-D3R (1:150 ...
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bioRxiv - Neuroscience 2021Quote: ... CeA and the PPTg were incubated in a chicken anti-GFP primary antibody (1:1000, Abcam, catalog# ab13970, lot# GR236651-13), then incubated in an Alexa Fluor 488-conjugated donkey anti-chicken secondary antibody (1:500 ...
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bioRxiv - Neuroscience 2021Quote: ... in PBS for 1 hour then incubated overnight with Anti-Amyloid beta precursor protein (Y188) rabbit monoclonal antibody at 1:1,000 (Abcam Cat# ab32136) and Anti-VCP mouse monoclonal antibody at 1:2,000 (Abcam Cat# ab11433) ...
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bioRxiv - Developmental Biology 2020Quote: ... for 30 mins at room temperature and incubated overnight with 1: 400 diluted rabbit anti-SYCP3 antibody (1:200, Abcam, ab15093) at 37°C ...
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bioRxiv - Cell Biology 2021Quote: ... with secondary antibody goat anti-mouse IgM mu chain (dilution 1:2000) conjugated with Alexa Fluor 488 (ab150121, Abcam, Cambridge, UK). Only one primary antibody was used in each experiment.
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bioRxiv - Cell Biology 2020Quote: ... Membranes were washed three times for 10 mins with TBST and incubated with IRDye conjugated antibodies (Goat anti-Rabbit IRDye800CW 1:5000 (abcam, #216773), donkey anti-mouse IRDye680RD 1:5000 (abcam ...
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bioRxiv - Immunology 2020Quote: ... Antigen-coupled beads were validated by incubating them with serial dilutions of anti-histidine-Biotin antibody for antigens with a histidine-tag (Abcam, ab27025). To choose an appropriate coupling concentration ...
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bioRxiv - Microbiology 2020Quote: ... Center for Therapeutic Antibody Discovery at the Icahn School of Medicine at Mount Sinai) and rabbit monoclonal anti-vinculin (Abcam, ab129002). Primary antibodies were detected by IRDye 680 Goat anti-Mouse IgG secondary antibody (926-68070 ...
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bioRxiv - Bioengineering 2021Quote: ... Primary antibody solutions were prepared by diluting one or more of the following antibodies in blocking buffer: mouse anti-HNF4a (1/200 from stock, Abcam ab41898), goat anti-OPN (1/60 from stock ...
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bioRxiv - Bioengineering 2021Quote: ... Secondary antibody solutions were prepared by diluting one or more of the following secondary antibodies in blocking buffer: DyLight 550-conjugated donkey anti-mouse IgG (1/50 from stock, Abcam, ab98767) and DyLight 488-conjugated donkey anti-goat IgG (1/50 from stock ...
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bioRxiv - Immunology 2021Quote: ... Expression of ACE2 was confirmed via immunoblot analysis (protocol described in Burke et al., 2019) using Anti-Angiotensin Converting Enzyme 2 antibody [EPR4435(2) (Abcam: ab108252) at 1:1000.
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bioRxiv - Microbiology 2021Quote: ... Proteins co-purified with His-NS5A or His tag protein were detected by western blot with mouse anti-Myc tag monoclonal antibody (Abcam, USA) for probing NEMO and mouse anti-His tag monoclonal antibody (Abcam ...
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bioRxiv - Microbiology 2021Quote: ... incubate the tissue with 70–90 µL of 1:350 secondary antibodies goat anti-chicken IgY Alexa Fluor-647 (ab150175, Abcam, USA) and 4.5µg/mL of Hoechst 33342 (ThermoFisher ...
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bioRxiv - Immunology 2020Quote: ... all serum samples were diluted to 1 total IgG ELISA unit and then detected with anti-mouse IgG subclass-specific secondary antibodies (Southern Biotech or Abcam). The results of the IgG subclass ELISA are presented using OD values instead of the ELISA units used for the total IgG ELISA ...
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bioRxiv - Genetics 2020Quote: ... Embryos were washed (3x 30 min PBS-T) and then secondary antibody (goat anti-mouse Alexa Fluor® 555 - abcam ab150114) at 1:2000 and a nuclear counterstain (TO-PRO™-3 Iodide ThermoFisher Scientific T3605 ...
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bioRxiv - Developmental Biology 2022Quote: Post-excysted bradyzoites and recrudesing populations were inoculated onto 6 well coverslips of HFF or primary astrocyte cells and at various times were fixed and stained using an anti-toxoplasma antibody (abcam, ab138698). Vacuole sizes at each timepoint were determined by counting 50 random vacuoles in triplicate on a Zeiss Axiovert fluorescent microscope.
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bioRxiv - Immunology 2022Quote: ... cells were washed with 1 ml of FACS buffer and resuspended in 500 µl of goat anti-rabbit Alexa Fluor 488 antibody (Abcam, ab150077) at 0.67 µg/ml ...
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bioRxiv - Microbiology 2022Quote: ... for 2 h at RT followed by two washed with PBS and 1:200 secondary antibody donkey anti–mouse-Alexa Fluor 568 (ab175700, Abcam, USA). To detect the total E-cadherin ...
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bioRxiv - Neuroscience 2022Quote: ... slices were incubated for 48 hours at 4 °C with primary antibodies diluted in 5% NGS in PBS-T as follows: rabbit anti-cFos (1:1000, Abcam, #190289) and chicken anti-GFP (1:1000 ...
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bioRxiv - Genetics 2022Quote: ... 500 ug lysate was incubated at 4□ overnight with 2 ug of appropriate antibodies (anti-H3K4me3 (Abcam, Cat# ab8580, RRID: AB_306649), anti-H3K36me3 (Abcam ...
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bioRxiv - Neuroscience 2022Quote: ... Sections were incubated at room temperature for ~3 hours in secondary antibody solution containing goat anti-rabbit IgG H&L Alexa Fluor 488 (abcam; ab150077).
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bioRxiv - Biochemistry 2022Quote: ... Detection of CMY-2 was performed by adding 500 ng/well of pAbs themselves followed by the addition of a 1/2000 diluted goat anti-rabbit antibody conjugated to HRP (Abcam, UK) or by adding 200 ng/well of monovalent or bivalent cAbCMY-2 (254 ...
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bioRxiv - Cancer Biology 2022Quote: ... for 1 h and then incubated overnight at 4 °C with primary antibodies: rabbit anti-human IRS2 (ab134101; Abcam, Cambridge, UK) and mouse anti-human active β-catenin (05-665 ...