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Citations for abcam :
7851 - 7900 of 10000+ citations for Recombinant Mouse Frizzled Homolog 1 Drosophila His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... slides were thoroughly washed using PBS 1X/Triton- X 0.1% and incubated overnight at 4°C with the respective antibodies anti-mouse Alexa-Fluor® 594 (abcam, ab150108), anti-sheep Alexa-Fluor® 488 (Jackson Immunoresearch ...
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bioRxiv - Microbiology 2022Quote: ... Spain) binding to the 229E nucleocapsid protein and a goat anti-mouse secondary antibody conjugated with fluorescein isothiocyanate (Abcam, United Kingdom). TCID50 titers were calculated according to the method of Reed and Muench (44).
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bioRxiv - Bioengineering 2022Quote: ... ECM components were detected using mouse mAbs specific for collagen type I (0.5 μg/ml clone ab90395, Abcam®, Cambridge, UK), collagen type IV (2 μg /ml clone M0785 ...
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bioRxiv - Cell Biology 2022Quote: Epidermal punch biopsies (6 mm diameter) in triplicates were floated on medium containing either a mouse α-RTN1A ab (mon162, abcam) or the respective isotype control ab (IgG1 ...
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bioRxiv - Cancer Biology 2022Quote: ... Coverslips were then washed with 1xPBS 3x for 5 minutes before incubation in 50 µl spots of secondary antibodies (goat anti-mouse AF647: Abcam ab150115, goat anti-rabbit AF405: abcam 175652) diluted in incubation buffer for 1hr at RT ...
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bioRxiv - Microbiology 2021Quote: ... horseradish peroxidase (HRP)-conjugated goat anti-mouse antibodies against the light chain (Fab) and heavy chain (Fc) (Abcam,plc, Cambridge, MA), and HRP-conjugated goat anti-rabbit antibody (Abcam ...
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bioRxiv - Neuroscience 2021Quote: ... and then incubated in secondary antibodies (donkey antichicken Alexa 488, Jackson ImmunoResearch 703-545-155; donkey anti-mouse Dylight 550, Abcam ab96876 ...
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bioRxiv - Immunology 2020Quote: Transferrin and cytokines in the samples (cell supernatant or plasma) were measured using a mouse transferrin ELISA kit (ab157724, Abcam, USA), mouse TNF-α ELISA kit (DKW12-2720-096 ...
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bioRxiv - Neuroscience 2021Quote: ... 0.1% SDS) and incubated overnight at 4°C with 3 μL specific mouse monoclonal antibody recognizing Bcl11a (Abcam Cat# ab19487, RRID:AB_444947) or unspecific IgG1 antibody (Cell Signaling Technology Cat# 5415 ...
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bioRxiv - Developmental Biology 2022Quote: Immunofluorescence was performed on serial sections along the rostral–caudal brain axis using the following antibodies: mouse monoclonal anti-GLUT1 (Abcam, ab40084), chicken polyclonal anti-GFP (Abcam ...
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bioRxiv - Developmental Biology 2019Quote: ... After washing 3 times with TBS-T the blots were incubated with HRP-conjugated secondary antibodies (goat-anti-mouse HRP (Abcam, ab6789); goat-anti-rabbit HRP (Abcam ...
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bioRxiv - Cancer Biology 2019Quote: ... Whole pancreas protein extracts were isolated and incubated with mouse inflammation/cytokine antibody arrays (40 targets, Abcam, ab133999; 97 targets, Abcam ab169820), according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: ... After blocking, the cells were stained with Mouse Anti-Human Myeloperoxidase (Santacruz, Germany) and Rabbit Anti-Human Histone-H3 (Abcam, USA) antibodies for one hour ...
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bioRxiv - Cancer Biology 2020Quote: ... The following primary antibodies and their respective dilutions were used in immunofluorescence (IF) and Western blot (WB) experiments: MITF (C5) mouse monoclonal (Abcam, #ab12039), 1:2000 (WB) ...
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bioRxiv - Immunology 2020Quote: ... Sections were incubated overnight at 4°C with a rabbit polyclonal anti-mouse blocking antibody (MUC2, ab90007, Abcam Ltd; Occludin, ab168986, Abcam Ltd; caspase 3, ab44976, Abcam Ltd). The sections were washed with PBS and incubated with a secondary antibody for 30 min ...
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bioRxiv - Cancer Biology 2021Quote: ... for 1hr and probed overnight with primary antibodies (Rabbit anti-SDMA, CST13222; mouse anti-vinculin, Sigma, RABBIT anti-PRMT5 (Abcam Ab31751). Blots were washed 3X with 1% TBST buffer and probed with secondary antibodies 680RD goat anti-Mouse and 800CW goat anti-Rabbit (LI-COR ...
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bioRxiv - Cancer Biology 2021Quote: ... sections with primary antibodies were incubated with secondary antibodies (Alexa Fluor-594 goat anti-rabbit IgG, #a21429, Thermo Scientific; Alexa Fluor 488 goat anti-mouse IgG, #ab-150116, abcam; Alexa Fluor 594 goat anti-mouse IgG ...
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bioRxiv - Microbiology 2020Quote: ... Anti-mouse IgG conjugated to HRP for immunoblot of immunoprecipitation samples (used in Fig. 3b) was purchased from Abcam (Cat#: ab131368). Fluorophore-conjugated secondaries for immunofluorescence were purchased from Life Technologies.
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bioRxiv - Microbiology 2021Quote: ... Hemoglobin and hemopexin levels in the plasma samples of WT- and KO-infected mice were measured by Mouse Hemoglobin (Abcam, ab157715) and Mouse Hemopexin (Abcam ...
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bioRxiv - Genetics 2020Quote: ... Embryos were washed (3x 30 min PBS-T) and then secondary antibody (goat anti-mouse Alexa Fluor® 555 - abcam ab150114) at 1:2000 and a nuclear counterstain (TO-PRO™-3 Iodide ThermoFisher Scientific T3605 ...
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bioRxiv - Developmental Biology 2022Quote: ... The cells were washed with 0.3% PBSTx four times for 15 min per wash and incubated in Alexa Fluor 488 Goat Anti-Mouse IgG H&L (Abcam, ab150117) at a 1:1000 dilution at room temperature (RT ...
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bioRxiv - Developmental Biology 2022Quote: ... β1-integrin blocking antibodies at 100 μg/mL (MilliporeSigma; # MABT821) or mouse monoclonal (P5D2) β1-integrin blocking antibodies at 50 μg/mL (Abcam; ab24693).
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bioRxiv - Physiology 2022Quote: ... and cells were washed in 3 changes of PBS prior to incubation with secondary antibody (DyLight 488 anti-rabbit IgG (ab96881) or DyLight 594 anti-mouse IgG (ab96881) (Abcam, UK)) for 1 hour at room temperature in the dark ...
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bioRxiv - Plant Biology 2022Quote: ... After three washes with 1 x PBS the samples were incubated for 4 hours with anti-mouse secondary antibodies conjugated with Alexa Fluor 488 (ab150077, Abcam, UK) diluted 1:400 in PBS ...
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bioRxiv - Neuroscience 2022Quote: The quantitative detection of serum lipopolysaccharide binding protein (LBP) was performed by Mouse LBP Enzyme-Linked Immunosorbent Assay (ELISA) kit (Abcam, ab213876) according to the manufacturer protocol ...
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bioRxiv - Physiology 2022Quote: ... Immunohistochemical staining for MMP13 was performed utilizing the Innovex Universal Animal IHC Kit and a polyclonal rabbit anti-mouse MMP13 antibody (Abcam ab39012). Briefly ...
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bioRxiv - Bioengineering 2023Quote: ... Then the secondary antibodies (Nissl 435/455, Thermo Fisher, N-21479, donkey anti-rabbit 488, Abcam, ab150061, donkey anti-mouse 568, Abcam, ab175700 ...
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bioRxiv - Neuroscience 2023Quote: ... was used for AnkB localization in cortical neurons in culture by immunofluorescence staining with mouse monoclonal anti-AnkB antibody 33-3700 and AF-594-conjugated secondary antibodies (Abcam, #ab245992). STED images were acquired using a Leica SP8 STED confocal microscope with Lightning deconvolution in the UNC Neuroscience Research Center.
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bioRxiv - Cancer Biology 2023Quote: ... Immunohistochemical SP method (according to Fuzhou Maishin immunohistochemical ultrasensitive mouse/rabbit SP IHC ready-to-use kit) was performed to detect the expression of CK2A1 (Abcam 76040), CK2A2 (Invitrogen PA5-109601) ...
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bioRxiv - Bioengineering 2023Quote: ... and Cytokeratin-14 (CAS: Ab119695) and the Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (CAS: Ab150113) were purchased from Abcam. CCK-8 and Live/Dead staining kit was supplied by Beijing Solarbio Science & Technology Co.,Ltd.
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bioRxiv - Microbiology 2022Quote: ... Antibodies used for immunostaining of plaque assays are: monoclonal anti-SARS-CoV-NP antibody (1C7C7) and anti-mouse HRP antibody (Abcam ab6823). Antibodies used for immunostaining of histology slides are ...
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bioRxiv - Immunology 2023Quote: ... Sections will then be stained with FITC-conjugated anti-C3 and Alexa Fluor 647-conjugated anti-mouse IgG (Abcam, Cambridge, UK) antibodies ...
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bioRxiv - Microbiology 2023Quote: ... Specific sIgA antibodies to the SARS-CoV-2 virus were detected with goat anti-mouse IgA alpha-chain + HRP (Abcam®), diluted to 1:1.000 ...
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bioRxiv - Biophysics 2023Quote: ... cells were incubated in a blocking buffer with 20 ng/μl of commercial secondary antibody goat anti-mouse IgG H&L (Alexa Fluor 647) (abcam, ab150115) for 45 min ...
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bioRxiv - Molecular Biology 2023Quote: ... Cultures were then washed 5x 5 minutes each and incubated with Alexa594-conjugated Goat α-Mouse IgG H&L (Abcam, #ab150116) diluted 1:500 in wash buffer for 1 hour at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... Secondary antibodies were used at a ratio of 1:500 in 5% GS/1% BSA/0.2% PBST (anti-rabbit-Alexa-Fluor-555, Invitrogen A27039; anti-mouse-Alexa-Fluor-647, Invitrogen A21235; anti-chicken-Alexa-Fluor, Abcam ab150169). Images were collected for analysis using a Keyence epifluorescence microscope with a 10X objective from a single plane of focus per section ...
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bioRxiv - Pathology 2023Quote: ... The rat monoclonal anti-mouse dipeptidyl peptidase-4 (Dpp4 also known as CD26) and the rat monoclonal anti-mouse F4/80 antibodies were purchased from Abcam (ab42899) and AbD serotec (CL:A3-1) ...
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bioRxiv - Neuroscience 2024Quote: ... secretory IFN-β in supernatant and 2’3’-cGAMP in primary cells (neuron, astrocytes, and microglia) lysate after Aβ or gAβ treatment were quantified by mouse IFN-β (abcam) or mouse 2’3’-cGAMP ELISA kit (Cayman Chemical ...
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bioRxiv - Microbiology 2024Quote: ... the cell lysate was split into equal volumes and combined with 2 µg mouse mAb anti-V5 (SV5-PK1) (Abcam, ab27671), mouse mAb anti-HA (clone HA-7)(Merck ...
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bioRxiv - Biochemistry 2024Quote: ... the wells were washed 6 times with wash buffer and then incubated with 100 µL per well of mouse biotinylated anti-DDDDK (Abcam ab173832) at 1:3000 in dilution buffer overnight at 4°C ...
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bioRxiv - Cell Biology 2024Quote: ... Primary antibodies were diluted in 5% BSA and incubated at 4°C overnight at given concentrations: α-smooth muscle actin (αSMA, mouse, Cat. No. ab7817, Abcam) at 1:300 dilution ...
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bioRxiv - Neuroscience 2024Quote: ... followed by incubation with appropriate secondary antibodies (anti-rabbit AlexaFluor-488, Invitrogen #A11008, anti-mouse AlexaFluor-568, Invitrogen #A11004, and anti-chicken AlexaFluor-647, Abcam #ab150175) for 1 hour at RT ...
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bioRxiv - Molecular Biology 2021Quote: ... Proteins were immunoprecipated from 1 μg chromatin and 1 μL of anti-H3 (Abcam, 1791) conjugated to 20 μl protein G magnetic beads (Invitrogen ...
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bioRxiv - Physiology 2020Quote: ... for 1 h at RT and primary RP anti-SOCS3 antibody (1:1000, ab16030, Abcam) or primary RP anti-PTP1B antibody (1:500 ...
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bioRxiv - Cell Biology 2019Quote: ... GFP 1:500 (Nacalai Tesque Cat# 04404-84) or NANOG 1:200 (Cat.#ab80892,Abcam) overnight at 4°C in blocking solution ...
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bioRxiv - Genomics 2019Quote: ... Primary antibodies included PODXL (AF1658; R&D; 1:500) and ECAD (ab11512; Abcam; 1:500). Stains included fluorescein-labeled LTL (FL-1321 ...
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bioRxiv - Genetics 2020Quote: ... Primary antibodies: γH2AX (1:400-500, MilliporeSigma, 05-636-I, LBR (1:100, Abcam, ab32535). Secondary antibodies ...
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bioRxiv - Pathology 2019Quote: ... The tissue was probed with rabbit polyclonal to ICAM-1 (1:400) antibody (Abcam, ab124759) and rabbit monoclonal to VCAM (1:600 ...
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bioRxiv - Neuroscience 2019Quote: ... Secondary antibodies were also incubated overnight (Alexa 488 1:1000, Abcam; Cy3 Stratech 1:500). The PGP antibody from Ultraclone was discontinued during our study ...
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bioRxiv - Molecular Biology 2019Quote: ... Histones were immunoprecipated from 1 μg chromatin and 1 μL of anti-H3 (Abcam, 1791) conjugated to 20 μl protein G magnetic beads (Invitrogen ...