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Citations for abcam :
7551 - 7600 of 10000+ citations for Anti ZNF699 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: ... cells were incubated for 2 hours with: (i) chicken polyclonal antibody anti microtubule-associated-protein 2 (MAP-2) (Abcam, #Ab5392) at dilution of 1/1000 in PBS containing 1% fetal calf serum and 0.1% of saponin (this antibody stains specifically cell bodies and neurites ...
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bioRxiv - Neuroscience 2021Quote: ... and incubated in 2% BSA in PBS to block against non-specific binding and incubated in 2% BSA containing rabbit anti-LC3B primary antibody (Abcam). Coverslips were then washed 3 × 5 minutes in PBS ...
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bioRxiv - Neuroscience 2021Quote: ... Free-floating sections in wells were treated with PV primary antibodies (rabbit anti-PV; 1:200; AB11427; Abcam, Cambridge, MA) and blue secondary antibodies (donkey anti-rabbit IgG conjugated to AlexaFluor 405 ...
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bioRxiv - Pathology 2021Quote: ... Membranes were incubated overnight at 4°C with the following antibodies: rabbit polyclonal anti-mouse MMP-13 (1/6000; ab39012; Abcam) and MMP-1 (1/500 ...
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bioRxiv - Cancer Biology 2021Quote: ... Unbound protein was removed by washing the wells three times with 100ul of washing buffer and bound Myc-HK2 was detected with monoclonal anti-Myc HRP-conjugated antibody (Abcam; 1:5000 in blocking buffer ...
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bioRxiv - Cancer Biology 2021Quote: Immunostaining of mouse lung sections was performed as described previously (Tavora et al., 2020) with the following antibodies: anti-Ki-67 (Abcam), anti-Endomucin (Santa Cruz Biotechnology) ...
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bioRxiv - Cancer Biology 2021Quote: ... They were detected with corresponding secondary antibody: i) donkey anti-sheep IgG H&L (HRP polymer) (catalog no. ab214884, Abcam) or ii ...
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bioRxiv - Cancer Biology 2021Quote: ... tissue sections were blocked with 5% mouse serum PBS1X for 1h30 and incubated with mouse anti-tryptase antibody (1:300, ab2378, RRID:AB_303023, ABcam, Cambridge, UK) for 1h at RT ...
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bioRxiv - Cell Biology 2020Quote: ... TZM-bl cells were similarly transfected followed by staining with 1:50 dilution of anti-mouse monoclonal CD4-APC conjugated antibody (Abcam) or isotype control (Abcam ...
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bioRxiv - Cell Biology 2020Quote: ... The wells were then washed three times with PBS buffer (0.05% tween) and treated with HRP-conjugated anti-FITC antibody (100 μL/well, Abcam, ab196968) for 1h at room temperature ...
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bioRxiv - Microbiology 2021Quote: ... The plate was washed three times with 1xPBS and then incubated with a fluorescence goat anti-mouse IgG heavy plus light chain (H+L)–Alexa Fluor 488 antibody (Abcam) or an anti-human secondary (Sigma ...
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bioRxiv - Genomics 2019Quote: ... and then incubated in anti-acetylated tubulin primary antibody (1:500, monoclonal, clone 6-11B-1, AB24610, Abcam, Cambridge, UK) and goat anti-mouse Alexa fluor 488 conjugate secondary antibody (1:1000 ...
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bioRxiv - Immunology 2021Quote: ... Plates were washed and bound specific IgG was detected with a HRP-conjugated goat anti-mouse IgG H&L antibody (ab6789, Abcam) diluted 1 ...
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bioRxiv - Molecular Biology 2020Quote: ... at room temperature for 2 h and then incubated overnight at 4°C in Blocking Buffer containing primary antibody (anti-mouse PIWIL2/MILI, Abcam Cat# ab36764 ...
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bioRxiv - Immunology 2021Quote: ... the membranes were washed with TBST and incubated with HRP-conjugated Goat-anti-human Fc antibody (Abcam, ab99759, 1:10000) for 1 h at RT ...
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bioRxiv - Cell Biology 2019Quote: ... of the anther cells was precleared with MagnaChiP A/G magnetic beads for 1 h at 4°C and incubated with anti-Sm antibodies (ANA No. 5 — 15 μl per sample, Y12 (Abcam) — 1 μg per sample ...
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bioRxiv - Cell Biology 2020Quote: Exosomes (25 μg prot) from A-375 cells were incubated or not with 5 µl anti-CD63 antibodies (Abcam, ab8219) for 2 h at room temperature ...
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bioRxiv - Genomics 2021Quote: ... 500 µL of supernatant fluid was added to 25 µL of pre-incubated protein G magnetic beads (ChIP-Adembeads ChIP-Adem-Kit, Ademtech SA) with 5 µg of anti-V5 rabbit polyclonal antibody (ab9116, Abcam) in IP buffer (50 mM HEPES-KOH pH 7.5 ...
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bioRxiv - Neuroscience 2021Quote: ... Tissue was incubated for 48 hr in primary antibody against tyrosine hydroxylase (anti-TH, raised in rabbit, 1:1000, Abcam) and green fluorescent protein (anti-GFP ...
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bioRxiv - Cell Biology 2021Quote: Supernatant and pellet samples were probed by Western Blot using the following antibody concentrations: 1:5000 anti-6XHis-HRP (Abcam), 1:5000 mouse anti-tubulin (Invitrogen) ...
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bioRxiv - Cell Biology 2021Quote: ... The co-immunoprecipitated protein complexes were detected using western blotting with the following antibodies: anti-TCFL5 (1:1000, ab188075, Abcam), anti-FXR1 (1:1000 ...
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bioRxiv - Cell Biology 2021Quote: ... HO-2 and HO-1 were probed with a 1:1,000 dilution of rabbit anti HO-2 antibody (Abcam 90515) or 1:1,000 rabbit anti HO-1 (Enzo Life Sciences BML- HC3001-0025 ...
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bioRxiv - Genomics 2021Quote: ... The fluorophore-conjugated secondary antibodies include Goat anti-Rabbit IgG H&L-Alexa Fluor 488 (1:500, Abcam, Cat#ab150077) and Goat anti-Rabbit IgG H&L-Alexa Fluor 555 (1:500 ...
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bioRxiv - Neuroscience 2021Quote: ... 10% DMSO) and put into primary antibody (PTwH, 5% DMSO, 3% donkey serum, Goat anti-GFP, Abcam ab5450, 1:1000) for 10 days at 37 degrees Celsius ...
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bioRxiv - Genomics 2020Quote: ... The membranes were probed with appropriate antibodies overnight at 4°C (anti-rabbit histone H3 (abcam, ab1791; 1: 2,500 dilution), anti-mouse FLAG antibody (Sigma ...
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bioRxiv - Neuroscience 2021Quote: ... Brains were incubated for at least 72 h with the primary antibodies anti-GFP (chicken, 1:2000; Abcam, Cambridge, UK), anti-nc82 (mouse ...
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bioRxiv - Neuroscience 2021Quote: ... specific for the glycosaminoglycan portion of the chondroitin sulfate proteoglycans that are the main components of the PNN and a polyclonal rabbit anti-parvalbumin antibody (1:1000; ab11427, Abcam). Sections were then incubated at room temperature in a mixture of secondary antibodies diluted in TBST ...
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bioRxiv - Neuroscience 2022Quote: ... and incubated for 3 hours at room temperature with the appropriate fluorophore-conjugated species-specific secondary antibody: Cy3 donkey anti-goat (1:1000, Abcam), Cy3 donkey anti-mouse (1:1000 ...
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bioRxiv - Neuroscience 2022Quote: ... Slices were then incubated for 48 hr at 4C in 1× PBS containing the primary antibodies (Chicken anti-GFP, AbCam cat ...
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bioRxiv - Neuroscience 2022Quote: ... Sections were then rinsed in PBS for 20 min before being placed in a blocking solution with secondary antibody used to visualize TH neurons in the LC (goat anti-rabbit Alexa Fluor 594, 1:1000 dilution, Abcam, catalog no ...
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bioRxiv - Neuroscience 2022Quote: ... The secondary antibodies for the immunohistochemistry or immunofluorescence staining and immunogold labeling were goat anti-mouse IgG (ABcam, Cat. #ab6789), Cy2 AffiniPure donkey anti-rabbit IgG (Jackon ImmunoResearch ...
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bioRxiv - Cell Biology 2022Quote: ... The following primary and secondary antibodies were used for tissue immunohistochemistry in this study: anti-perilipin (Abcam; ab3526; 1:200) and goat anti-rabbit Alexa Fluor 488 (ThermoFisher ...
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bioRxiv - Developmental Biology 2022Quote: Secondary antibodies were generally diluted at 1:200 and included the following: Alexa Fluor 488 conjugated donkey anti-goat (Abcam); Alexa Fluor 555 conjugated donkey anti-rabbit (Abcam) ...
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bioRxiv - Microbiology 2022Quote: ... then were washed and was incubated with 1:200 secondary antibody donkey anti–mouse-Alexa Fluor 568 (ab175700, Abcam, USA). The non–permeabilized samples stained for accessible E-cadherin were permeabilized with PBS-0.2% Triton 100-X for 10 min at RT ...
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bioRxiv - Microbiology 2022Quote: ... Bacteria harboring an HA tag were washed with PBS and stained with anti-HA antibody conjugated with FITC (Abcam ab1208) at 10 µg/mL for 75 minutes in the dark on ice ...
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bioRxiv - Neuroscience 2022Quote: ... Secondary antibodies were then applied (IB4-Alexa 647, 1:300, ThermoFisher I32450; goat anti-mouse Alexa-488, 1:500, Abcam ab150113 ...
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bioRxiv - Cancer Biology 2022Quote: ... The following antibodies were used with a total of 5 µg of each primary antibody used per immunoprecipitation: Rabbit anti-LSD1 (ab17721, Abcam), Rabbit ani-MYC (13987 ...
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bioRxiv - Bioengineering 2022Quote: ... and a cocktail of biotinylated detection antibody (~1 ng/ml per each protein panel) and phycoerythrin (PE, 532 nm emission) conjugated anti-human IgG antibody (10 ng/ml, Abcam) was loaded onto the barcoded array slide after removing excess serum using BSA solution ...
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bioRxiv - Bioengineering 2022Quote: ... BD FACS Canto II was used to analyze VCAM-1 positive cells using FTIC- anti-VCAM-1 antibody (CC# ab24853; Abcam) and the number of positive cells were quantified using with FlowJo software (Tree Star) ...
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bioRxiv - Biochemistry 2022Quote: ... The assay was revealed by a 1/2000 diluted goat anti-rabbit antibody conjugated to horseradish peroxidase (HRP) (Abcam, UK). All steps were followed by 5 washes with 50 mM pH 7.5 buffer with 0.05 % tween-20 ...
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bioRxiv - Bioengineering 2022Quote: ... and subsequently incubated in primary antibody solution (1:200 CD31 Dako IS610 + 1:200 CD10 Invitrogen PA5-85875 or 1:200 anti-laminin Abcam ab11575 or 5 μg/mL ZO-1 Invitrogen #61-7300 ...
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bioRxiv - Developmental Biology 2022Quote: ... Tissue sections were incubated overnight at 4°C in a humidified chamber with primary antibodies: rat anti-BrdU (1:500; Cat#: ab6236, RRID:AB_305426; Abcam, Cambridge, UK), mouse anti-HuC/D (1:500 ...
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bioRxiv - Developmental Biology 2022Quote: ... The preparation was rinsed with PBS and blocked overnight in 2.5% non-fat dry milk in PBS and then treated with an anti-centrin antibody (1/1000th anticentrin 1 ab11257; Abcam) at 20°C overnight ...
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bioRxiv - Developmental Biology 2022Quote: The following secondary antibodies were used at 1:500 dilutions in this study: goat anti-rat IgG Fc (FITC) (ab97089, Abcam), goat anti-rabbit IgG (H+L ...
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bioRxiv - Genetics 2022Quote: ... The cleared lysate was incubated at 37 °C for 30-mins and subjected to Western blot analysis using an anti-GFP antibody (Abcam) to detect POPDC1-CFP containing complexes ...
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bioRxiv - Plant Biology 2022Quote: ... a Western blot was performed to visualize the GFP-tagged heterologously expressed proteins and actin as a loading control with respectively an α-GFP polyclonal (A-6455; Thermo) primary antibody at a 1:2,000 dilution and an anti-Actin (ab197345, abcam) primary antibody at a 1:5,000 dilution ...
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bioRxiv - Microbiology 2022Quote: ... A polyclonal anti-GFP-antibody (A-11122, Thermo Fischer Scientific) was used with a horseradish peroxidase-conjugated secondary anti-rabbit IgG (Ab6721, Abcam) and Clarity Western ECL substrate (Bio-rad ...
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Evaluation of the DBA/2J mouse as a potential background strain for genetic models of cardiomyopathybioRxiv - Physiology 2022Quote: ... blocked in 5% BSA-PBS at room temperature and incubated with the anti-syntrophin primary antibody (1:2000; #11425; Abcam) overnight at 4°C ...
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bioRxiv - Microbiology 2022Quote: ... Tween-20 and 5% (w/v) milk powder containing 1:5,000 horseradish peroxidase-conjugated goat anti-mouse IgG secondary antibody (AbCam ab205719) and incubated on a shaking platform for 1 hr at room temperature ...
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bioRxiv - Microbiology 2022Quote: ... Membranes were then washed 3x with PBST and incubated 1 hr at 22°C in 100 µL anti-mouse IgG AlexaFluor 594 secondary antibody (ab150116, Abcam) diluted 1:200 in PBST + 1% bovine serum albumin + 10% goat serum ...