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Citations for abcam :
701 - 750 of 864 citations for n4 Benzoyl 5 methyldeoxycytidine since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... unskimmed milk powder, and incubated with mouse monoclonal anti-HA (clone 6E2, Cell Signalling Technology) and anti-p24 (clone 5, Abcam) overnight at 4°C ...
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bioRxiv - Immunology 2024Quote: The proliferation of CD3+ T-cell was assessed using the 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) (Abcam, US) following the manufacturer’s protocol [11] ...
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bioRxiv - Microbiology 2024Quote: ... To test for O-x-AlkTDM presence we used in-gel fluorescence (to detect TAMRA) and/or immunoblotted with anti-biotin antibodies (1:1000; 5% nonfat Milk in TBST; Abcam).
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bioRxiv - Microbiology 2024Quote: Anti-HIV-1 Pr55Gag + p24 + p17 antibody (ab63917) and anti-tubulin antibodies (B-5-1-2) were purchased from Abcam and Sigma-Aldrich ...
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bioRxiv - Immunology 2024Quote: ... ∼50 µg of lysate material was treated with the reducing agent TCEP at a final concentration of 5 mM and heated to 60 °C for 30 minutes before being loaded onto a FASP protein digestion column (Abcam)75 ...
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bioRxiv - Immunology 2024Quote: ... Cells were permeabilized with 0.1% Triton X-100 / 2% BSA in 1x PBS for 5 min and F-actin was stained by Phalloidin-iFluor488 (1:1,000, Abcam #ab176753) for 1h room temperature prior to slides mounting (Fluoromount/DAPI).
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bioRxiv - Immunology 2024Quote: ... Slides were washed 3 x 5 min and stained with 1:1 HRP-conjugated goat anti-rabbit IgG (Abcam ab214880) in 1% BSA-TBS for 2 hours at room temperature ...
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bioRxiv - Cell Biology 2024Quote: ... The membranes were blocked with 5% milk followed by incubation with primary antibodies overnight using the following antibodies: anti-VEGF-A (Abcam), anti-BCL2 (Cell Signaling Technology) ...
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bioRxiv - Molecular Biology 2024Quote: ... Wells were again washed three times for 5 minutes with dPBS before incubating with secondary antibodies (goat anti-rabbit IgG Alexa Fluor 488 [Abcam] ...
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bioRxiv - Cancer Biology 2024Quote: ... or with recombinant TGF-β at 10 ng/mL (stock concentration 5 ng/µL) or Batimastat at 30 µM (stock concentration 1 mM, BB-94/Abcam). After 48 hours (or 1 hour for SMAD activity tests) ...
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bioRxiv - Molecular Biology 2024Quote: ... 3 µl of 1 mM DCFDA dye in 1X buffer and 5 µl of 10 µM DiOC2 was added for ROS evaluation (ab113851, Abcam) and membrane depolarization (B34950 ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were then washed for 5 mins in PBS and mounted with mounting media containing DAPI (catalog number ab104139, Abcam) before imaging.
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bioRxiv - Bioengineering 2024Quote: ... the blots were washed with PBS supplemented with 0.1% Tween 20 four times for 5 min before staining with the secondary antibody (7076, Cell Signaling; ab216773, Abcam) in blocking buffer with 0.1% Tween 20 for 1h at room temperature ...
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bioRxiv - Physiology 2024Quote: ... The membranes were blocked (5% milk (PanReac AppliChem, A0830)) and probed with the following antibodies: anti-β-Tubulin (Abcam, ab15568), anti-GAPDH (Proteintech ...
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bioRxiv - Bioengineering 2024Quote: ... cells were washed 3 times for 5 min with PBS before incubating with a secondary antibody (ab150080 and ab150157, Abcam) for 1 h at room temperature ...
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bioRxiv - Cancer Biology 2024Quote: ... All of supernatant containing digested nuclear extract was transferred to a new tube and 500µl of Low IP buffer without additional NaCl or detergent was added together with 5-10µg of KAT7 antibody (Abcam #190908) or rabbit IgG (R&D Systems ...
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bioRxiv - Bioengineering 2024Quote: ... for 5 minutes and then incubated for 1 hour at room temperature (RT) in NM95 primary antibody (ab190710 16 - Abcam) at 1:1500 in TBS ...
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bioRxiv - Bioengineering 2024Quote: Cell proliferation was measured via metabolic activity using a 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) assay kit (Abcam, UK). Briefly ...
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bioRxiv - Cancer Biology 2024Quote: ... Membranes were blocked with 5% milk-TBST for 1 hour under agitation and probed overnight for ALIX (1:1000, 186429, Abcam), Calnexin (1:1000 ...
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bioRxiv - Cell Biology 2024Quote: ... The primary antibody was washed 2x with PBS for 5 min per wash and cells were incubated with secondary antibodies (diluted in 0.5% BSA/PBS) at room temperature in the dark for 1 h at 1:1000 dilution (anti-rabbit Alexa488, Abcam ab150077 ...
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bioRxiv - Microbiology 2020Quote: The mutant library was screened by preparing a 1 × 10-6 dilution in LB medium and plating on LB agar containing 60 µg ml-1 X-Gal (5-bromo-4-chloro-3-indolyl-β-D-galactopyranoside, Abcam) and 500 µM IPTG ...
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bioRxiv - Molecular Biology 2021Quote: ... The membrane was blocked in 5% milk for 1h followed by incubation with primary antibodies: c-Myc (Abcam ab185656; 1:1000), Fos (Cell Signaling 2250S ...
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bioRxiv - Developmental Biology 2021Quote: ... Sections were blocked with 5% donkey serum and incubated overnight at 4°C against MMP13 (1:100, Abcam ab39012, Cambridge, UK) and for 2 hours at room temperature with a Cy3-conjugated secondary (1:500 ...
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bioRxiv - Neuroscience 2022Quote: ... then incubated for 2 d at 4°C in primary rabbit anti-TH antibody (Abcam, 1:200, in TBSMT + 5% DMSO). After 6 washes x 1 h with TBST ...
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bioRxiv - Neuroscience 2021Quote: ... injected with vehicle (VEH = 0.9% aqueous solution of NaCl and 5% ethanol) or MTZ (10 mg/kg, ab120068, Abcam, Cambridge, UK). The 10 mg/kg daily dosage in mice is equivalent to 50 mg/day in humans ...
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bioRxiv - Biochemistry 2020Quote: ... Membranes were treated with antibodies diluted 1:1000 in 0.5% BSA and washed 3 times for 5 min before applying secondary antibiotic (anti-mouse or anti-goat from Santa Cruz Biotechnology and Anti-Rabbit from Abcam at a concentration of 1:1000 ...
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bioRxiv - Immunology 2021Quote: ... The membrane was then washed 3×15 minutes in PBST and then incubated in secondary antibody solution (PBST + 5% BSA + Goat a-Rabbit-HRP 1:5000) (Abcam, ab205718) for one hour at room temperature ...
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bioRxiv - Neuroscience 2020Quote: ... The blots were blocked in TBST buffer containing 5% nonfat milk and then incubated overnight at 4°C with the primary rabbit polyclonal anti-Tip60 (1:1000, abcam, #ab23886), rabbit polyclonal anti-Rpd3 (1:1000 ...
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bioRxiv - Bioengineering 2021Quote: ... blocked with 5% goat serum and incubated overnight with primary polyclonal rabbit antibodies in 5% goat serum at 1:150 dilution for types I (Abcam AB34710), II (Abcam AB34712) ...
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bioRxiv - Cell Biology 2021Quote: ... Afterwards membranes were blocked for 30 min with 5% Nutrilon (Nutricia) in PBS and incubated overnight at 4°C with primary antibodies H3 (Abcam 1791), H4 (Merck Millipore 04-858) ...
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bioRxiv - Immunology 2021Quote: ... Cells were washed in PBS for 5 min before being blocked with 10% normal donkey serum (Abcam, ab7475, Lot GR3234297-32) diluted in PBS for 20 minutes at room temperature ...
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bioRxiv - Immunology 2021Quote: ... Fixed cells were blocked with 5% BSA in PBS for 1 hr at room temperature and incubated with rabbit anti-mouse Ki67 primary antibody (Abcam ab15580) at 1:200 dilution overnight at 4°C ...
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bioRxiv - Cancer Biology 2021Quote: ... Tissue samples were washed with PBS three times for 5 minutes each before adding a secondary goat anti-rabbit IgG H & L (1:1000, Alexa Fluor® 555, Abcam) and Goat Anti-Rat IgG H & L (1:1000 ...
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bioRxiv - Cancer Biology 2022Quote: ... Samples were sectioned in the sagittal plane to a thickness of 5 μm prior to immunoperoxidase staining for Nestin-1 (Abcam, ab237036), a marker that is used to detect vessels in AOM-induced tumors41,42.
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bioRxiv - Neuroscience 2022Quote: ... in a heating chamber (37°C) in which the choroid plexus was loaded with Fluo-8 AM (5 μM, 30 min, Abcam, ab142773) with subsequent continuous wash with HEPES-aCSF (~1 ml/min ...
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bioRxiv - Immunology 2020Quote: ... The transferrin levels in cells were tested by Western blotting using anti-transferrin antibody (1:5□000 dilution, ab82411, Abcam, USA) as described above ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were blocked and permeabilized with 0.5% TBS-T and 5% BSA and incubated overnight at 4°C with primary antibodies anti-MAP-2 (1:500, Abcam; ab32454) and anti-GFAP (1:500 ...
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bioRxiv - Microbiology 2021Quote: ... Membranes were then washed with TBST buffer (5 × 1 min) and probed with secondary rabbit anti-rat antibodies conjugated with peroxidase (1:1000, Abcam #ab6734). Blots were washed as described above and detection was carried out using Amersham ECL Western Blotting Select Detection Reagent (GE Lifesciences #RPN2235) ...
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bioRxiv - Neuroscience 2022Quote: ... spinal transverse sections 5 mm caudal and rostral from the lesion center were prepared and stained for BDA or PKCγ (Abcam, ab109539). For mice with uPx ...
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bioRxiv - Cell Biology 2022Quote: ... deparaffinized sections were rehydrated and stained using specific antibodies against Leucine-rich repeat-containing G-protein-coupled receptor 5 (Lgr5, ab219107, Abcam, USA), Lysozyme (ab76784 ...
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bioRxiv - Genomics 2021Quote: ... were used to capture 400 μL of SUMO-2/3 antibody (8A2, acquired from Abcam, ab81371; ∼5-10 μg/μL antibody). All washing and handling steps were followed by centrifugation of the beads at 500g for 3 min in a swing-out centrifuge with delayed deceleration and careful aspiration of buffers ...
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bioRxiv - Immunology 2020Quote: ... was used to solubilize N-(1-(2′-(4-Isopropoxyphenoxy)-2,5’-bithiazol-5-yl)ethyl)acetamide (“ACC2 inhibitor 1”; ab142090; purchased from Abcam, Cambridge, UK), 5-[1’-(1-cyclopropyl-4-methoxy-3-methylindole-6-carbonyl)-4-oxospiro[3H-chromene-2,4’-piperidine]-6-yl]pyridine-3-carboxylic acid (“ACC2 inhibitor 2” known as MK-407439 ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... which was blocked with 5% (w/v) skimmed milk powder in PBS and probed for ERα at a concentration of 1:1000 (Abcam ab108398) and PRβ at a concentration of 1:1000 (Abcam ab46535 ...
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bioRxiv - Neuroscience 2023Quote: ... The sections were then incubated overnight at 4°C in primary antibody diluted in blocking solution with 5% normal goat serum (Abcam, ab7481). Antibodies were directed against GFP (Abcam ...
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bioRxiv - Cancer Biology 2023Quote: ... Samples were pre-cleared using the beads provided in the kit and incubated overnight at 4°C with 5 µg of commercially available antibodies against lamin A/C (Abcam, #ab108595), phospho-Ser301 lamin A/C (Affinity ...
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bioRxiv - Microbiology 2023Quote: ... washed further 5 times with PBS pH 7.4 and incubated for one hour with Goat anti-monkey (Rhesus macaque) (Abcam cat # a112767), diluted 1:2000 in PBS pH 7.4 ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... paraffin-embedded and 5 µm-thick sections were stained with hematoxylin and eosin (H&E) or picrosirius red solution (Abcam #ab150681) as in [18] ...
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bioRxiv - Microbiology 2023Quote: ... 5 s at 60% amplitude) in 320 µL of buffer A containing 2 % (v/v) NP-40 (Abcam, Cat. No. ab142227). The aggregated proteins were sedimented at 15,000 x g for 30 min at 4 °C ...
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bioRxiv - Systems Biology 2023Quote: ... Primary antibodies were diluted in 5% milk TBST and incubated with blots overnight at 4 °C (FGF1 – Abcam, ab207321; β-ACTIN – Abcam, ab8226 ...
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bioRxiv - Neuroscience 2022Quote: ... The rats received bilateral lesions in the LHb with ibotenic acid (5 µg/µl in 0.01 M PBS, Abcam, Cambridge, UK). The coordinates for the LHb (AP −3.3 mm ...