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Citations for abcam :
701 - 750 of 10000+ citations for Nuclear pore complex protein Nup93 dye Antibody Biotin since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2021Quote: ... Membranes were blocked in 3% skim milk for 2 hours and then incubated with primary antibody overnight at 4°C for the following proteins: 4HNE (Abcam, ab46545), β-actin (Santa Cruz Biotech) ...
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bioRxiv - Neuroscience 2021Quote: ... constant shaking) with the following primary antibodies: sheep anti-phospo-S6 ribosomal protein (pS6; 1:250, ab65748, Abcam, Cambridge, Massachusetts, USA); mouse anti-D3R (1:150 ...
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bioRxiv - Neuroscience 2021Quote: ... in PBS for 1 hour then incubated overnight with Anti-Amyloid beta precursor protein (Y188) rabbit monoclonal antibody at 1:1,000 (Abcam Cat# ab32136) and Anti-VCP mouse monoclonal antibody at 1:2,000 (Abcam Cat# ab11433) ...
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bioRxiv - Microbiology 2021Quote: ... Proteins co-purified with His-NS5A or His tag protein were detected by western blot with mouse anti-Myc tag monoclonal antibody (Abcam, USA) for probing NEMO and mouse anti-His tag monoclonal antibody (Abcam ...
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bioRxiv - Genomics 2021Quote: ... Fragmented DNA was then diluted 10 times with ChIP dilution buffer and protein-DNA complexes were captured using an H3K27ac antibody (Abcam AB4729). Next ...
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bioRxiv - Cancer Biology 2020Quote: S-nitrosylation of proteins was detected by western blot using an S-nitrosylation specific antibody (HY8E12 clone, Abcam # 94930, 1:20) or the Pierce S-Nitrosylation Western Blot detection kit (Thermo Fisher Scientific # 90105) ...
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bioRxiv - Neuroscience 2022Quote: ... NF200 (1:500, Chemicon International, Temecula, CA, USA) rabbit monoclonal amyloid precursor protein (APP) antibody [Y188] (1:500, Abcam, Cambridge, MA) and NeuN (1:500 ...
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bioRxiv - Genomics 2022Quote: ... The ChIP assay was performed with Dynabead Protein G beads and histone antibodies (H3K4me3: Millipore #07-473, H3K4me1: Millipore #07-436, H3K27me3: Millipore #07-449, H4K27ac: abcam ab4729). After binding to antibodies ...
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bioRxiv - Neuroscience 2023Quote: ... After SDS-PAGE and proteins transferred on PVDF membranes were incubated overnight at 4°C with Total OXPHOS Human WB Antibody Cocktail (Abcam, UK) and subsequently detected with HRP-conjugated goat anti-rabbit IgG secondary antibody (1:4000 ...
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bioRxiv - Physiology 2023Quote: ... Frozen sections were fixed with fresh 4% paraformaldehyde and neutrophil elastase protein was detected using anti-neutrophil elastase antibody (Abcam #68672).
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bioRxiv - Neuroscience 2023Quote: ... CTIP2 (chicken ovalbumin upstream promoter transcription factor-interacting protein) antibody was added (rat, 1:500, Abcam, Cat: ab18465, Lot: GR3272266-13) to facilitate delineation of striatum and incubation continued until the 4 hours were completed ...
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bioRxiv - Immunology 2022Quote: ... The slides were incubated with primary rabbit anti-SARS nucleocapsid protein antibody(NB100-56576, Novus, USA) overnight at 4°C and then with goat anti-rabbit IgG HRP (Abcam, UK) for 1 hr ...
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bioRxiv - Developmental Biology 2023Quote: ... nucleoplasm and chromatin fractions were used for protein analysis with western blotting with the following antibodies were used: anti-DHX9 (Abcam 26271), anti-TBP (Abcam 818) ...
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bioRxiv - Cancer Biology 2022Quote: Protein array analysis was performed using the Human MAPK Phosphorylation Antibody Array (ab211061) according to the manufacturer’s instructions (Abcam, Boston, MA). Visualization was performed with the ChemiDoc Touch Imaging System (Bio-Rad ...
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bioRxiv - Genomics 2023Quote: ... The heterozygous expression of the Dam-Lamin B1 fusion protein was further confirmed by Western blot using an anti-HA antibody at 1 in 5000 dilution (Abcam, ab9110) and an anti-LaminB1 antibody at 1 in 5000 dilution (Abcam ...
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bioRxiv - Neuroscience 2023Quote: ... the cryosections of human brain were mounted on a Polyethylene naphthalate (PEN) Membrane Slide (Zeiss, 415190-9041-000) and then stained with spike protein antibodies using the IHC kit (Abcam, ab210062). After further processing ...
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bioRxiv - Immunology 2024Quote: ... and stained for proteins of interest using the following primary and secondary antibodies: S100A8 (clone: EPR3554, 1:100, Abcam, cat# ab92331), goat anti-rabbit IgG Alexa Fluor 594 (1:500 ...
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bioRxiv - Molecular Biology 2024Quote: ... previously blotted membranes from Figure S2C,E were re-probed with 1:5000 mouse monoclonal anti-TATA binding protein antibody (Abcam #ab51841). The membrane in Figure S2H was probed for 1 h with 1:1000 rabbit anti-LARP7 antibody (Fortis/Bethyl #A303-723A ...
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bioRxiv - Microbiology 2024Quote: ... and a rabbit antibody recognizing cellular proteins or epitope tags (anti-EEA1, Cell Signaling Technology, 2411, 1:75; anti-TGN46, Abcam, ab50595 ...
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bioRxiv - Molecular Biology 2024Quote: ... The membrane was then probed with a polyclonal rabbit anti-human antibody targeting human AL light chain protein (Abcam, 1:500). A horseradish peroxidase-conjugated goat anti-rabbit IgG (Invitrogen ...
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bioRxiv - Biochemistry 2024Quote: ... Eluted proteins were resolved on NuPAGE™ 4–12% Bis–Tris gel followed by Western blotting using anti-IFNAR1 antibody (ab124764, Abcam).
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bioRxiv - Cell Biology 2024Quote: ... SR-BI protein was determined by Western blotting by probing cell lysates with SR-BI antibody (ab52629, Abcam, 1:1000 dilution).
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bioRxiv - Plant Biology 2024Quote: ... The proteins were also visualised by total protein staining with Instant Blue® Coomassie Protein Stain (Abcam ab119211). For Western blot analysis ...
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bioRxiv - Physiology 2020Quote: ... Protein concentrations were measured using BCA Protein Assay Reagent (Abcam, USA). Proteins were separated on SDS-PAGE and transferred to the polyvinylidene fluoride (PVDF ...
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bioRxiv - Bioengineering 2024Quote: ... Protein mass was determined using the BCA protein assay kit (Abcam) with PierceTM bovine serum albumin as a standard (Thermo Scientific).
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bioRxiv - Cell Biology 2020Quote: ... rabbit anti-biotin (ab53494, WB 1:1000), mouse anti-integrin beta 1 (ab30394, IF 1:400) all from Abcam; rabbit anti-Rabankyrin-5 was a gift from Professor Marino Zerial (Max Planck Institute of Molecular Cell Biology and Genetics ...
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Development of monoclonal antibody-based blocking ELISA for detecting SARS-CoV-2 exposure in animalsbioRxiv - Microbiology 2023Quote: ... Biotinylation of the mAb was performed using a Biotin Conjugation Kit by following the manufacturer’s instruction (Abcam, Cambridge, MA). The SARS-CoV-2 N-specific mAb B6G11 was previously developed in Diel lab (20).
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bioRxiv - Molecular Biology 2021Quote: ... Cells were incubated either with phalloidin conjugated to Alexa 488 dye (Phalloidin-iFluor 488, Abcam ab176753) following the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2024Quote: ... glucose 13.8 mM) and loaded with a cell permeable fluorescent calcium sensitive dye Fluo8-AM (Abcam) at 5 μM and allowed for 30 min incubation at 37°C ...
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bioRxiv - Cancer Biology 2021Quote: ... Equal amounts of total proteins as determined by BCA Protein Assay were analyzed by SDS-polyacrylamide gel electrophoresis with antibodies specific to IL-8 (Cat. No. 18672, Abcam, Cambridge, MA) and prostate specific antigen (Cat ...
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bioRxiv - Neuroscience 2021Quote: ... slices were then incubated at 4 °C overnight in the primary antibodies: chicken anti-glial fibrillary protein GFAP (targeting astrocytes, 1:200, Abcam #ab4674, USA), goat anti-ionized calcium binding adaptor molecule 1 (Iba1 ...
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bioRxiv - Neuroscience 2021Quote: ... slices were then incubated at 4 °C for 5-7 days in the primary antibodies containing: chicken anti-glial fibrillary acidic protein (GFAP) (targeting astrocytes, 1:200, Abcam #ab4674, USA) and/or and NeuN (targeting nuclei of neurons ...
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bioRxiv - Neuroscience 2021Quote: ... the brain slides were incubated with primary antibody rabbit monoclonal anti-amyloid precursor protein (APP) [clone Y188] (#AB32136, Abcam, USA; 1:500) in blocking solution at 4 °C overnight ...
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bioRxiv - Molecular Biology 2021Quote: ... The membrane was then washed thrice with 1X-TBST and incubated with anti-SARS-CoV-2 N protein antibodies (catalogue No. ab272852, Abcam, 1:1000) in TBST with BSA (2% ...
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bioRxiv - Cell Biology 2021Quote: ... The cells were then fixed with 4% paraformaldehyde and processed for immunofluorescence analysis using GAP SH3 Binding Protein 1 (G3BP1) antibody (green; Abcam, Cambridge, MA) and fluorophore-conjugated secondary antibody ...
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bioRxiv - Animal Behavior and Cognition 2022Quote: ... Astrogliosis was visualized after staining with rabbit Monoclonal Anti-Glial Fibrillary Acidic Protein (GFAP) antibody at a 1:1000 dilution (Abcam, Cambridge, MA). All samples were analyzed on an inverted epifluorescent microscope (Olympus IX71) ...
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bioRxiv - Neuroscience 2020Quote: ... Slices were incubated over night with primary antibody rabbit-anti-Ca2+/calmodulin-dependent protein kinase II (1:200, #PA5-38239, Thermo Fisher, MA, USA; 1:500, #ab52476, Abcam, UK), rabbit-anti-parvalbumin (1:500 ...
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bioRxiv - Biochemistry 2021Quote: ... and analyzed by western blot using anti-HBP1 (11746-1-AP, Protein Tech Group, 1:500) or anti-ZMYND19 antibody (ab86555, Abcam, 1:500).
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bioRxiv - Neuroscience 2020Quote: Lysates from DRG neurons were run on 4-12% Bis-Tris gradient gels and proteins were transferred to PVDF membranes that were incubated with antibodies to REST (Abcam, 1:1000), using anti-β-actin as a loading control ...
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bioRxiv - Pathology 2021Quote: ... tissue proteins (20 μg) were separated by 4-20% SDS-PAGE and immunoblots were developed using antibodies against pSMAD3 (Abcam, 1:1000), SMAD3 (Abcam ...
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bioRxiv - Biochemistry 2021Quote: ... The homogenate was clarified by centrifugation at 21,130 x g for 15 minutes before analysis of protein levels in the supernatant by immunoblotting using antibodies including anti-PKA pRIIα (Abcam, RRID: AB_779040), anti-GFP (Sigma Aldrich ...
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bioRxiv - Immunology 2021Quote: ... Cells were then stained for intracellular spike protein using an Anti-SARS-CoV-2 Spike Glycoprotein S1 antibody (Abcam, ab275759, 1:50) followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor 488 ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... a pair of monoclonal antibodies (clone EPR16588, ab178846; clone EPR16589, ab178847) and a recombinant human protein (ab105593) are available from Abcam (Waltham, MA). Similarly ...
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bioRxiv - Molecular Biology 2023Quote: ... They were then washed with PBS and incubated with 1° antibodies (α-NDUFV2, 15301-1-AP, Protein tech, α-tubulin, Abcam, ab179513) in 5% milk 0.1% Tween 20 ...
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Apicosome: newly identified cell-type-specific organelle in mouse cochlear and vestibular hair cellsbioRxiv - Neuroscience 2022Quote: The following primary antibodies were used in this study: two rabbit polyclonal antibodies against human fibronectin leucine-rich transmembrane protein 1 (FLRT1) (ab97825 and ab103839; Abcam, Cambridge, UK), rabbit polyclonal anti-human FLRT2 (ab154023 ...
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bioRxiv - Immunology 2022Quote: ... or CD8+ cells using a rabbit-raised anti-SARS-CoV-2 S protein antibody (1:5000 dilution, ab272504, Abcam plc, Cambridge UK), a rabbit monoclonal anti-CD4 antibody (1:250 dilution ...
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bioRxiv - Cell Biology 2024Quote: ... Detection of thiophosphate ester tagged proteins from the in vitro kinase assay was done with a specific anti-thiophosphate ester antibody (#ab92570 Abcam 1:10000). Antibody against total CDC-42 was use for the loading control (#sc-390210 ...
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bioRxiv - Genomics 2024Quote: ... we extracted the total protein from the mutant cells and conducted western immunoblotting using a recombinant anti-HA tag antibody (Abcam, Waltham, MA).
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bioRxiv - Physiology 2024Quote: ... The freely floating tissue were then incubated with specific combinations of primary antibodies [astrocytes - glial fibrillary acidic protein - GFAP (chicken, ab4674, abcam, 1:500), alpha synuclein (mouse ...
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bioRxiv - Genetics 2024Quote: ... Proteins were transferred to PVDF membranes by wet transfer and incubated with specific primary antibodies: anti-γ-H2A (ab15083, Abcam, 1:1,000), anti-Rad53 (ab166859 ...