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Citations for abcam :
701 - 750 of 10000+ citations for 6 oxo 1 phenyl 1 4 5 6 tetrahydro pyridazine 3 carboxylic acid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2020Quote: ... cells were fixed with 4% paraformaldehyde then immunostained for Runx2 (Abcam ab76956, 1:250), followed by incubation with a fluorescently conjugated secondary antibody (ThermoScientific ...
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bioRxiv - Cell Biology 2022Quote: ... and then rotated overnight at 4°C with 1 μg of H3K4me3 (Abcam, ab1012), 2 μg H3K4me1 (Abcam ...
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bioRxiv - Neuroscience 2023Quote: ... then incubated overnight at 4 °C in primary antibodies: mouse anti-Beclin 1 (Abcam), mouse anti-GluN2B (BD Transductions) ...
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bioRxiv - Bioengineering 2023Quote: ... hepatic nuclear factor 4 alpha (HNF4a, hepatocyte marker; 1:100; Abcam Cat# ab41898, RRID:AB_732976), and phosphorylated c-Jun (pcJun ...
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bioRxiv - Cancer Biology 2023Quote: ... Samples were incubated with primary antibodies overnight at 4 °C (1:100, Abcam #ab32072), then washed with PBS five times and incubated overnight with Alexa Fluor 568 - conjugated anti-rabbit secondary antibodies (1:500 ...
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bioRxiv - Developmental Biology 2023Quote: ... and incubated overnight at 4°C with rabbit anti-Sox2 (1:100; Abcam ab97959) or mouse anti-HuC (1:400 ...
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bioRxiv - Molecular Biology 2024Quote: ... After overnight incubation at 4°C with rabbit anti-NR2F2 (1:2000, #ab42672, Abcam) diluted in TBS-T-5% BSA ...
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bioRxiv - Cancer Biology 2024Quote: ... overnight at 4 °C and secondary antibody (rabbit anti-mouse, Abcam ab46540, 1:100) for 1 hour at room temperature.
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bioRxiv - Genomics 2023Quote: ... Beads were then resuspended in 1 mL BSA solution and 5 μL of α-5-hmU antibody (Abcam Cat # ab19735) were added ...
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bioRxiv - Developmental Biology 2021Quote: Apoptosis and proliferation levels were measured by immunostaining in dewaxed and rehydrated midline placental sections with cleaved caspase-3 (Asp175) (Cell Signalling, US, 9661; 1:200) and Ki67 (Abcam, UK, ab264429; 1:500). Sections were incubated with goat-anti-rabbit secondary antibody (Abcam ...
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bioRxiv - Developmental Biology 2019Quote: ... Membranes were blocked in 5% milk for 1 hr at room temperature and incubated with primary DAZL antibody (ab215718, Abcam, 1:1000) overnight at 4 °C ...
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bioRxiv - Systems Biology 2022Quote: ... Cells were tehen incubated with a primary antibody solution containing BrdU antibody diluted 1:400 or 1:1000 in PBS containing 5% goat serum (Abcam, catalog # ab7481) and 0.1% Triton-X for 1 hour at room temperature ...
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bioRxiv - Biochemistry 2023Quote: ... Stripped membranes were blocked in 5% NFDM in TBST (1 h, RT) before being probed with anti-Act1 (Abcam ab8224, 1:5000) loading control antibody (1 h ...
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bioRxiv - Cancer Biology 2023Quote: ... The membrane was blocked with 5% skim milk for 1 hour at RT before immunoblotting with antibodies against ACC1 (#ab72046, Abcam, 1:2000), ACC2 (#HPA006554 ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... washed three times in 1XPBS for 5’ and incubated with anti-neural/glial antigen 2 (NG-2, 1:100, in 1XPBS + 1%NGS, Abcam, Cambidge, UK), overnight at 4°C.
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bioRxiv - Bioengineering 2023Quote: ... which was prepared by 5% milk in TBS-T containing 1:10,000 horseradish peroxidase (HRP)-conjugated goat anti-mouse secondary antibodies (Abcam ab6789-1 MG). The secondary antibody incubation was applied for 1 hour at room temperature ...
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bioRxiv - Pathology 2021Quote: ... The membrane was then probed overnight at 4°C with primary antibodies (P105/p50, 1:1000, Abcam ab32360; Actin, 1:1000, Abcam ab3280) in Odyssey Blocking Buffer and 0.1% Tween-20 ...
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bioRxiv - Immunology 2020Quote: ... and mouse anti-human HSC70/HSP70 (clone N27F3-4, ENZO dilution 1:1.000, sample reduced with β-mercapthoethanol) and rabbit anti-human lactoferrin (polyclonal, Abcam, dilution 1:5.000), rabbit anti-human TLR3 (D10F10 ...
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bioRxiv - Developmental Biology 2022Quote: ... 0.1% Triton X-100 in PBS at room temperature and incubated overnight at 4°C with primary antibodies: α-actinin (Abcam; 1:300), GLI2 (Novus biologicals ...
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bioRxiv - Molecular Biology 2022Quote: ... in PBST for 1 h at room temperature and then immunoblotted overnight at 4 °C with corresponding primary antibodies: anti-FTO (Abcam, ab124892, 1/1000) or anti-β-Actin (Sigma ...
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bioRxiv - Molecular Biology 2023Quote: ... The membrane was blocked with 5% skim milk for 1 hour at room temperature and then were incubated overnight at 4°C with the anti-MGST3 antibody (Abcam, ab192254; 1: 1000). Subsequently it was incubated with horseradish peroxidase (HRP)-conjugated secondary antibody (Cell signaling ...
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bioRxiv - Cell Biology 2023Quote: ... The membranes were blocked in 5% skim milk solution for 1 h at RT then incubated with primary antibody at 4 ℃ overnight: Anti-FMRP antibody (Abcam, ab17722, 1:1,000), anti-GAPDH antibody (Cell signaling technology ...
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bioRxiv - Neuroscience 2023Quote: ... The slides with cryosectioned tissue were washed with PBS followed by incubation in blocking solution (2% of BSA in PBS) for 1 h and then incubated overnight at 4°C with an anti-DBI antibody (rabbit, 1:1000, Abcam, Cat. No: ab231910). Sections were then incubated for 1 h at room temperature with anti-rabbit Alex Fluor 546 (1:1000 ...
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bioRxiv - Neuroscience 2021Quote: Acute slices containing Neurobiotin-filled cells were fixed 30 min in 4% paraformaldehyde at 4°C and immunolabelled with antityrosine hydroxylase (chicken polyclonal, Abcam, 1:1000) and anti-AnkyrinG (mouse monoclonal IgG2b ...
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bioRxiv - Cell Biology 2020Quote: ... Eluted proteins were analyzed on a 6% SDS-PAGE gel followed by a western blot using monoclonal anti-GST (Abcam) or anti-MBP (New England Biolabs ...
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bioRxiv - Immunology 2020Quote: ... 6 µg of pre-cleared chromatin was immunoprecipitated with 2 µg/IP anti-Histone H3K27Ac (Abcam Cat#ab4729; RRID: AB_2118291), 4 µg/IP of anti-LXRα/β (provided by Knut Steffenssen ...
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bioRxiv - Neuroscience 2020Quote: ... The IgG amount in that reference sample was interpolated as an average across exposures from a concentration curve calculated in GraphPad Prism 6 from a blot of known concentrations (IgG from Abcam ab133470 ...
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bioRxiv - Cancer Biology 2021Quote: ... for 6 h and then fixed with methanol for immunostaining with an antibody against nuclear pore complex proteins (clone mAb414, Abcam) following the regular immunofluorescence protocol described below ...
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bioRxiv - Cell Biology 2021Quote: Iron saturation of transferrin was measured on 6% TBE-urea gels as previously described.32 Gels were stained with InstantBlue Coomassie protein stain (abcam).
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bioRxiv - Immunology 2021Quote: ... and hLIGHT-6×His tag proteins (made by the methods described above) in the mixtures with anti-6×His tag PE-labeled antibody (Abcam) for 20 min on ice ...
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bioRxiv - Immunology 2022Quote: ... Each well of a 6-well plate was incubated for 30min at room temperature with 200μL of Cell Extraction Buffer PTR (ab195215, ABCam, UK) containing phosphatase inhibitors and the protease inhibitor aprotinin ...
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bioRxiv - Neuroscience 2024Quote: ... coli), monoclonal Xarr1-6 anti-SAG (Peterson et al., 2003) and monoclonal G8 anti-GRK1 (Zhao et al., 1998)(Abcam). Images were obtained using a Zeiss 510 meta confocal microscope equipped with a 40X N.A ...
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bioRxiv - Cell Biology 2022Quote: ... 10mg replicate 2) were incubated for 4 h at 4°C with 5 µg anti-GFP antibody (Abcam ab290) or mock-treated as a control ...
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bioRxiv - Cell Biology 2023Quote: ... GRASP65 was then depleted by treatment with 0.5 mM of the auxin indole-3-acetic acid (IAA, Abcam) for 2 h (Zhang and Seemann ...
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bioRxiv - Neuroscience 2021Quote: ... was used to detect intracellular HTT fibrils and caspase-3 activity in untreated and treated neurons was monitored by immunocytochemistry using anti-caspase-3 antibody (Abcam, Cat# 32351, 1:1000). Images were captured using a Leica SP8 confocal microscope and analyzed by LAS X software ...
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bioRxiv - Immunology 2019Quote: ... pylori antibody (in-house) and anti-cadherin (5 μg/ml; clone DECMA-1, Abcam), followed by the appropriate secondary antibodies ...
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bioRxiv - Bioengineering 2021Quote: ... Double staining immunofluorescence (IF) was performed for cytokeratin 5 (K5, ab52635, Abcam, 1:200) and cytokeratin 10 (K10 ...
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bioRxiv - Cancer Biology 2020Quote: ... Primary antibodies (diluted in 5% milk) used were: anti-γH2AX (Abcam ab11174; 1:5000), anti-KillerRed (Evrogen AB961 ...
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bioRxiv - Developmental Biology 2019Quote: The following antibodies were used: rabbit anti-Aquaporin 5 (AQP5, 1:2500, ab78486, Abcam), goat anti-Carbonic anhydrase IV (CAR4 ...
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bioRxiv - Molecular Biology 2019Quote: ... Primary antibodies (CD31, sc-1506, 1 µg/ml, Fibrinogen, abcam, ab34269, 5 µg/ml) were incubated at 4°C overnight ...
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bioRxiv - Cell Biology 2020Quote: ... blocked in 5% milk and probed with the following antibodies: NPM1 (Abcam, 1:1000), IPO7 (Abcam ...
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bioRxiv - Developmental Biology 2021Quote: ... Primary antibodies were incubated overnight at 40C in 5% NDS/PBS (Nkx2-1 [abcam Cat ...
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bioRxiv - Cell Biology 2021Quote: ... Smc4 was probed using a rabbit polyclonal antibody (Abcam, ab229213, GR3228108-5, 1:1000). GAPDH was probed with a rabbit polyclonal antibody (Abcam ...
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bioRxiv - Neuroscience 2023Quote: ... 5-HT1A receptor antagonist (10-5M WAY-100635 maleate, Abcam, CAS:634908-75-1), 5-HT1A receptor agonist (10-5M Ipsapirone ...
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bioRxiv - Immunology 2023Quote: ... or VeriBlot Detection Reagent (#ab131366, 1:1000 in tris saline/5% skim milk; abcam). Protein expression was visualized by enzymatic chemiluminescence using PierceTM ECL western blotting substrate (#32106 ...
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bioRxiv - Molecular Biology 2024Quote: ... and Recombinant Alexa Fluor 647 anti-Cytokeratin 5 antibody diluted 1:200 (Abcam, ab193895) and DAPI (Sigma-Aldrich ...
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bioRxiv - Microbiology 2023Quote: ... Prior to DENV-2 infection cells were pre-treated with 1 µM fatty acid synthase (FAS) inhibitor C75 (Abcam) or DMSO diluent only for 24 hours ...
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bioRxiv - Immunology 2021Quote: ... and rabbit polyclonal ab5103 against histone 3 (citrulline R2+R8+R17) at 1:100 dilution (abcam). Secondary antibodies were horseradish peroxidase-conjugated antibodies using the goat anti-mouse or anti-rabbit enhanced polymer two-Step detection system (ZSGB-Bio ...
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bioRxiv - Neuroscience 2021Quote: ... rabbit anti-LIMB HOMEOBOX CONTAINING 3 (LHX3) (1/100; Abcam; Toronto, ON, Canada; Cat. No. ab14555); goat anti-FORKHEAD BOX PROTEIN 1 (FOXP1 ...
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bioRxiv - Physiology 2019Quote: ... A 3:1 molar ratio of Fab to antibody target was used for CD133 (AbCam ab19898), and 6:1 molar ratio for FLK-1 (AbCam ...