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Citations for abcam :
701 - 750 of 10000+ citations for 1 Ethylbenz cd indol 2 1H ylidene malononitrile since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2022Quote: ... This was followed by a 2-hour incubation with secondary antibody goat anti-rabbit Alexa Fluor 488 (1:500, #ab150077, Abcam, Waltham, MA, USA), and a 0.1% (w/v ...
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bioRxiv - Neuroscience 2021Quote: ... brains were incubated in primary antibodies (diluted in 5% serum in PBT at 4°C for 2–4 days): Chicken anti-GFP (Abcam ab13970; 1:1000); anti-TH mouse monoclonal (SIGMA MAB318 ...
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bioRxiv - Physiology 2020Quote: ... diluted in PBTD was added for 2 hours followed by an anti-elastin rabbit polyclonal antibody diluted in PBTD 1:100 (Abcam, Cambridge, UK; ab21610). The samples were incubated with the primary antibody for 18 hours at 4°C followed by 30 minutes washing in PBTD ...
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bioRxiv - Bioengineering 2021Quote: ... This was followed by a 2-hour incubation with secondary antibody goat anti-rabbit Alexa Fluor 488 (1:500, #ab150077, Abcam, Waltham, MA, USA), and a 0.1% (w/v ...
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bioRxiv - Physiology 2021Quote: ... 4µm sections of paraffin-embedded mouse kidneys were submitted to antigen retrieval for 20 min at 95°C in citrate buffer followed by incubation with Ly-6B.2 antibody (Abcam, ab53457, 1:100). Sections were incubated with HRP-labeled secondary antibody (Vector ...
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bioRxiv - Immunology 2020Quote: The HSV-seropositivity status of donors completing the survey was confirmed by ELISA using the anti-herpes simplex virus type 1 and 2 IgG human ELISA kit (abcam, Cambridge, United Kingdom) according to manufacturer’s instructions.
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bioRxiv - Molecular Biology 2022Quote: ... blocked with blocking solution at 37°C for 3 h and incubated for 2 h with primary antibody (H3K9ac – Abcam ab12179, 1:300) and 1 hour with secondary antibodies (Donkey anti mouse – Abcam ab150109 ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... N-[4-chloro-3-(trifluoromethyl)phenyl]-2-ethoxy-6-pentadecyl-benzamide (CTPB) (CAS #: 586976-24-1) (>99% purity) was purchased from Abcam (Cambridge, United Kingdom). For both chemicals ...
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bioRxiv - Bioengineering 2024Quote: ... Secondary staining was performed for 2 hours at RT in a PBS (1x) solution containing secondary antibody-TRIC labeled (Abcam, MA, 1:1000), BSA (1% ...
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bioRxiv - Cancer Biology 2023Quote: ... The NOS2 antibody complex was stripped by heating with Bond Epitope Retrieval 2 and then stained with CD8 (Abcam no. 101500, 1:100) or IFNγ (Abcam no ...
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bioRxiv - Cancer Biology 2023Quote: ... The NOS2 antibody complex was stripped by heating with Bond Epitope Retrieval 2 and then stained with CD8 (Abcam no. 101500, 1:100) or IFNγ (Abcam no ...
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bioRxiv - Cell Biology 2023Quote: ... 1:400 dilution) and anti-α- synuclein aggregate antibody (Clone#: MJFR-14-6-4-2; 1:1000 dilution) were acquired from Abcam (Cambridge UK).
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bioRxiv - Neuroscience 2023Quote: ... DRG or spinal sections were incubated with secondary antibodies for 2 h: goat anti-mouse IgG H&L (Alexa Fluor® 488) (1:500, Abcam, Cat#: ab150113), goat anti-guinea pig IgG H&L (Alexa Fluor® 488 ...
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bioRxiv - Bioengineering 2024Quote: ... Then the membrane was washed three times in TBS-T and incubated at room temperature for 2 hours with the following secondary antibodies goat anti-rabbit IgG H&L (HRP) (Abcam #ab205718, 1:2000) and m-IgG Fc BP-HRP (Santa Cruz ...
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bioRxiv - Molecular Biology 2024Quote: ... Whole cell lysates of SH-SY5Y cells (10 µg) were subjected to Western blotting using the following antibodies: mouse anti-SLP-2 antibody (Abcam ab89025, 1:1000), rabbit anti-HSP60 (Cell Signaling #4870 ...
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bioRxiv - Molecular Biology 2024Quote: ... midguts were incubated at room temperature for 2 hours on an orbital shaker with secondary antibodies (goat anti-rabbit IgG Alexa Fluor 488 [Abcam] at 1:4000) diluted in midgut blocking buffer ...
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bioRxiv - Physiology 2024Quote: ... and 0.3% Triton X-100 in PBS for 2 h at room temperature and incubated with primary c-FOS antibody (rabbit, Abcam ab190289, 1:1000) overnight at 4°C ...
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bioRxiv - Genomics 2021Quote: ... serial sections were DAB stained with 2 µg/mL of anti-SARS-CoV-2 spike glycoprotein antibody (Abcam, ab272504). Slides were scanned at 20x directly on the NanoString GeoMx instrument to facilitate alignment with subsequent WTA/CTA/Protein scans ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were incubated in treatment media for the indicated period of time and then MTS reagent ((3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium) purchased from Abcam, (ab197010 ...
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bioRxiv - Microbiology 2024Quote: ... tachyzoites were stained with 2 µL of Annexin-V-FITC and 2 µL of propidium iodide (ab 14085, Abcam) in 100 µL annexin-V buffer (ab… Abcam ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... then incubated overnight at 4°C with the following primary antibodies in 2% w/v BSA: Rabbit anti-Gclc antibody (Abcam, United Kingdom; 1:1000); rabbit anti-NQO1 antibody (Cell Signalling ...
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bioRxiv - Neuroscience 2022Quote: ... Membranes were blocked with 1×TBST containing 5% dry milk for 2-hr at room temperature and probed with the following antibodies: anti-Kv7.4 antibodies (#ab84820, Abcam, Cambridge, United Kingdom; 1:500), anti-REST antibodies (#ab21635 ...
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bioRxiv - Neuroscience 2020Quote: ... incubated in PBST containing 2% NGS with goat anti-rabbit secondary antisera conjugated to Alexa Fluor® 488 (1:1000, Abcam, Cambridge UK, #ab150169) and Alexa Fluor® 647 (1:1000 ...
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bioRxiv - Cell Biology 2023Quote: ... Cells lysates were either mixed with 3 × Laemmli buffer for immunoblotting analysis or subject to pre-clearing and NDC80 complexes were isolated from 1 mg of cell lysate by 2-h incubation at 4°C with anti-NDC80 antibody cocktail (ProteinTech, 18932-1-AP; Abcam, ab3613; SantaCruz, sc-515550) or ...
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bioRxiv - Microbiology 2022Quote: ... The higher molecular weight part of the membrane was incubated overnight at 4ºC in anti-DNAK antibody (1:5000 dilution in blocking buffer, Abcam#69617 Mouse monoclonal [8E2/2]) as the loading control ...
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bioRxiv - Neuroscience 2024Quote: ... in PBS before incubation with the primary antibody MJF-14 (anti-confor-mation-specific α-synuclein filament antibody MJFR-14-6-4-2, Abcam, #ab209538, 1:25,000), overnight at 4°C ...
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bioRxiv - Neuroscience 2024Quote: ... fol-lowed by 5% horse serum before incubation with the primary antibody MJF-14 (anti-conformation-spe-cific α-synuclein filament antibody MJFR-14-6-4-2, Abcam, #ab209538, 1:200). The immunohistology sig-nals were visualized with ImmPRESS®-AP Horse Anti-Rabbit IgG Polymer (VectorLabs ...
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bioRxiv - Neuroscience 2024Quote: ... before blocking in 10% BSA and incubation with primary anti-bodies against aggregated α-synuclein (MJF-14; rabbit MJFR-14-6-4-2, Abcam, #ab209538, 1:25,000), total α-synuclein (mouse LB509 ...
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bioRxiv - Plant Biology 2024Quote: ... kC9 and its phosphonic acid ester form (P-[[(Acetyloxy)methoxy]-2-naphthalenylmethyl]-bis[(acetyloxy)methyl] ester phosphonic acid: 1) are also commercially available (abcam AB141566 and abcam141567, respectively). These compounds were diluted in DMSO and subjected for chemical treatment ...
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bioRxiv - Genomics 2021Quote: ... and sheared DNA was added to antibodies coupled to washed magnetic beads (anti-FLI1 2 µg, ab15289, Abcam; anti-ERG 2 µg, ab92513, Abcam; anti-H3K4me3 1.4 µg ...
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bioRxiv - Neuroscience 2020Quote: ... Western blot membrane stripping for restaining was performed for p-ERK1/2 and ERK1/2 antibodies using a mild stripping protocol from abcam. Briefly ...
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bioRxiv - Microbiology 2021Quote: ... PBS for 45 minutes and further incubated in blocking solution containing anti-SARS-CoV-2 spike protein subunit 2 (Abcam) 1:1000 dilution for 1 hour at 37°C ...
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bioRxiv - Immunology 2021Quote: ... SARS-CoV-2 infected cells were visualized using SARS-CoV-2 S-protein specific immunochemistry staining with anti-SARS-CoV-2 spike glycoprotein S1 antibody (Abcam) 90.
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bioRxiv - Biochemistry 2021Quote: ... coli were purchased and added to Kinase Buffer I (25 mM MOPS pH7.2, 12.5 mM β-glycerol phosphate, 25 mM MgCl2, 5 mM EGTA, 2 mM EDTA, 0.25 mM DTT; Abcam ab189135). Either water (mock ...
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bioRxiv - Biochemistry 2021Quote: Mid-log stage TbGT10 cKO cultures (∼1 × 106 cells/mL) were passaged to 2 × 103 cells/mL or 2 × 104 cells/mL and dosed with 100 nM rapamycin (Abcam) from a 1 mM stock solution in DMSO ...
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bioRxiv - Microbiology 2022Quote: ... tween 20 in PBS (PBST) three times followed by incubation with goat F(ab’)2 anti-human IgG (Fab’)2-horseradish peroxidase (Abcam) at a 1:10,000 dilution ...
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bioRxiv - Neuroscience 2023Quote: ... Cell viability was assessed after 72 hours using a 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) assay kit (Abcam, ab197010) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2024Quote: ... were passaged to 2 x 103 cells/mL or 2 x 104 cells/mL and dosed with 100 nM rapamycin (Abcam) from a 1 mM stock solution in DMSO ...
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bioRxiv - Microbiology 2023Quote: ... 2 µg of rabbit anti-CCT3 (Ray Biotech, 144-06547-200) or 2 µg IgG isotype control antibody (Abcam, ab172730) and incubated at 4°C for 30 min with rotation ...
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bioRxiv - Immunology 2024Quote: The proliferation of CD3+ T-cell was assessed using the 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) (Abcam, US) following the manufacturer’s protocol [11] ...
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bioRxiv - Biophysics 2024Quote: ... Coverslips were then blocked for 1 hour in a blocking solution (PBS with 2% BSA and 2% fish gel) and then incubated with anti-amyloid beta primary antibody (DE2B4) from Abcam diluted 1/400 in the blocking solution ...
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Decreased Astrocytic CCL5 by MiR-324-5p Ameliorates Ischemic Stroke Injury via CCR5/ERK/CREB PathwaybioRxiv - Neuroscience 2024Quote: ... The PVDF membranes were blocked with 5% BSA and probed with primary antibodies (phospho-ERK1/2, Abcam, ab76299; ERK1/2, Abcam, ab184699 ...
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Decreased Astrocytic CCL5 by MiR-324-5p Ameliorates Ischemic Stroke Injury via CCR5/ERK/CREB PathwaybioRxiv - Neuroscience 2024Quote: ... The PVDF membranes were blocked with 5% BSA and probed with primary antibodies (phospho-ERK1/2, Abcam, ab76299; ERK1/2, Abcam, ab184699; phospho-CREB, Abcam, ab32096 ...
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bioRxiv - Bioengineering 2024Quote: Cell proliferation was measured via metabolic activity using a 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) assay kit (Abcam, UK). Briefly ...
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bioRxiv - Cell Biology 2022Quote: ... or goat anti-Calnexin (2 μg/ml, Abcam). After incubation with the secondary antibody ...
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bioRxiv - Biochemistry 2020Quote: ... or Anti-DnaJB8 (rabbit) (abcam; ab235546; GR3229943-2) in milk and incubated the membrane shaking at room temperature for 2 hours ...
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bioRxiv - Cell Biology 2020Quote: ... anti-Tubulin [YL1/2] Rat monoclonal (Abcam, ab6160), anti-CHMP4B (Proteintech ...
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bioRxiv - Immunology 2021Quote: ... 2) rabbit anti-human CD3 (clone SP7, abcam) with rhodamine Red-X donkey anti-rabbit IgG (Jackson Immuno) ...
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bioRxiv - Genomics 2021Quote: ... rabbit monoclonal [ER179(2)] anti-AR (#ab108341, Abcam), mouse monoclonal [F6] anti-probasin (#sc-393830 ...
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bioRxiv - Bioengineering 2021Quote: ... 4′,6-diamidino-2-phenylindole (DAPI, Abcam, ab228549) was diluted to 2.5 µM in PBS and added to the cells for 15 minutes in the dark at room temperature ...