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Citations for abcam :
651 - 700 of 10000+ citations for PAS Domain Containing Serine threonine Protein Kinase PASK Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2019Quote: ... cells were resuspended at a concentration of 2×106 cells/ml in serum-free media containing 30 μg/ml of an IgG mouse isotype control antibody (abcam, Cambridge, MA, Catalog #ab91353) or a function blocking anti-β1 integrin antibody (abcam ...
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bioRxiv - Biochemistry 2021Quote: ... Resin was washed 4X in 800 uL EMB 300 Buffer and then protein association was analyzed by Western blot using antibody for hnRNP A2/B1 (Abcam #ab6102). Additionally ...
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bioRxiv - Neuroscience 2019Quote: ... Sections were blocked with 5% fish gelatine before incubation with the primary antibodies for myelin basic protein (MBP, Abcam, 1:500) and protein gene product 9.5 (PGP ...
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bioRxiv - Systems Biology 2020Quote: ... The blot was probed with the antibody against the viral nucleocapsid protein (Cambridgebio, USA) and the horseradish peroxidase-conjugated Goat Anti-Rabbit IgG (Abcam, USA) as the primary and the secondary antibodies ...
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bioRxiv - Cell Biology 2020Quote: ... the PNBM treated cell lysates were analyzed with a western blot assay to measure protein thio-phosphorylation with the anti-thiophosphate ester rabbit monoclonal antibodies (Abcam, USA).
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bioRxiv - Genetics 2020Quote: ... Reactions were stopped by addition of 6x SDS Laemmli buffer and proteins were resolved by 15% SDS-PAGE and analysed by western blotting with anti-ubiquitin antibodies (1:2000 Abcam P4D1) and anti-Rad51 (1:5000 ...
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bioRxiv - Neuroscience 2021Quote: ... The monoclonal anti-VGLUT2 antibody was raised in Mice immunized to a full length recombinant protein corresponding to rat VGLUT2 (Abcam, ab79157) and has been previously shown to stain VGLUT2 in zebra finches (86) ...
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bioRxiv - Neuroscience 2020Quote: ... at 100 μm thickness (coronal) and processed for free floating immunofluorescence using chicken anti Green-Fluorescent-Protein (GFP) primary antibody (1:1000 AbCam, ab13970), and Goat anti Chicken-alexa488 secondary antibody (1:500 thermofisher ...
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bioRxiv - Microbiology 2020Quote: ... The cells were then probed for 1 h with a polyclonal rabbit anti-SARS-CoV-2-S protein antibody (1:2,250; ab252690; Abcam, Cambridge, UK) or a rabbit anti-MeV N protein antibody (1:1,000 ...
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bioRxiv - Cancer Biology 2021Quote: ... Blots were blocked for 1 hour at room temperature with 5% fat-free milk or milk-free blocking buffer (for analysis of phosphorylated proteins) and incubated overnight at 4°C with primary antibodies for p-GCN2 (Abcam, ab75836), p-eIF2α (Cell Signaling ...
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bioRxiv - Microbiology 2022Quote: ... Spain) binding to the 229E nucleocapsid protein and a goat anti-mouse secondary antibody conjugated with fluorescein isothiocyanate (Abcam, United Kingdom). TCID50 titers were calculated according to the method of Reed and Muench (44).
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bioRxiv - Microbiology 2020Quote: ... endogenous human NPC1 and HSP90 were purified using immobilized Recombinant Protein G Resin (Generon) and 4 µg of specific antibodies against NPC1 (Abcam, ab108921) or HSP90 (Enzo Life Sciences ...
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bioRxiv - Microbiology 2020Quote: ... 50 μg of recombinant Rv3351c protein and anti-6xHIS antibody conjugated to horseradish peroxidase (HRP, 1:500; Abcam Inc., Cambridge, MA) were mixed together in PBS containing 0.5% BSA ...
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bioRxiv - Cancer Biology 2021Quote: Protein extraction and immunoblotting was performed according to standard procedures with primary antibodies against FLI1 (1:500, rabbit, ab15289, abcam, UK) and GAPDH (1:5000 ...
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bioRxiv - Developmental Biology 2019Quote: Phospho-protein staining was performed as previously described (Okuda et al. 2018) using the following antibodies: GFP (abcam cat. no. ab13970), pErk1/2 (cell signaling technologies cat ...
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bioRxiv - Cancer Biology 2019Quote: ... Whole pancreas protein extracts were isolated and incubated with mouse inflammation/cytokine antibody arrays (40 targets, Abcam, ab133999; 97 targets, Abcam ab169820), according to manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: ... The proteins of interests were detected using the following primary antibodies: anti-rabbit Catalase (1:375; catalog #Ab52477; Abcam, Cambridge, MA); anti-rabbit Glutathione Peroxidase (1:500 ...
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bioRxiv - Physiology 2021Quote: ... Membranes were blocked in 3% skim milk for 2 hours and then incubated with primary antibody overnight at 4°C for the following proteins: 4HNE (Abcam, ab46545), β-actin (Santa Cruz Biotech) ...
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bioRxiv - Neuroscience 2021Quote: ... constant shaking) with the following primary antibodies: sheep anti-phospo-S6 ribosomal protein (pS6; 1:250, ab65748, Abcam, Cambridge, Massachusetts, USA); mouse anti-D3R (1:150 ...
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bioRxiv - Neuroscience 2021Quote: ... in PBS for 1 hour then incubated overnight with Anti-Amyloid beta precursor protein (Y188) rabbit monoclonal antibody at 1:1,000 (Abcam Cat# ab32136) and Anti-VCP mouse monoclonal antibody at 1:2,000 (Abcam Cat# ab11433) ...
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bioRxiv - Microbiology 2021Quote: ... Proteins co-purified with His-NS5A or His tag protein were detected by western blot with mouse anti-Myc tag monoclonal antibody (Abcam, USA) for probing NEMO and mouse anti-His tag monoclonal antibody (Abcam ...
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bioRxiv - Genomics 2021Quote: ... Fragmented DNA was then diluted 10 times with ChIP dilution buffer and protein-DNA complexes were captured using an H3K27ac antibody (Abcam AB4729). Next ...
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bioRxiv - Cancer Biology 2020Quote: S-nitrosylation of proteins was detected by western blot using an S-nitrosylation specific antibody (HY8E12 clone, Abcam # 94930, 1:20) or the Pierce S-Nitrosylation Western Blot detection kit (Thermo Fisher Scientific # 90105) ...
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bioRxiv - Immunology 2022Quote: ... The slides were incubated with primary rabbit anti-SARS nucleocapsid protein antibody(NB100-56576, Novus, USA) overnight at 4°C and then with goat anti-rabbit IgG HRP (Abcam, UK) for 1 hr ...
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bioRxiv - Neuroscience 2023Quote: ... After SDS-PAGE and proteins transferred on PVDF membranes were incubated overnight at 4°C with Total OXPHOS Human WB Antibody Cocktail (Abcam, UK) and subsequently detected with HRP-conjugated goat anti-rabbit IgG secondary antibody (1:4000 ...
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bioRxiv - Neuroscience 2022Quote: ... NF200 (1:500, Chemicon International, Temecula, CA, USA) rabbit monoclonal amyloid precursor protein (APP) antibody [Y188] (1:500, Abcam, Cambridge, MA) and NeuN (1:500 ...
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bioRxiv - Genomics 2022Quote: ... The ChIP assay was performed with Dynabead Protein G beads and histone antibodies (H3K4me3: Millipore #07-473, H3K4me1: Millipore #07-436, H3K27me3: Millipore #07-449, H4K27ac: abcam ab4729). After binding to antibodies ...
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bioRxiv - Physiology 2023Quote: ... Frozen sections were fixed with fresh 4% paraformaldehyde and neutrophil elastase protein was detected using anti-neutrophil elastase antibody (Abcam #68672).
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bioRxiv - Developmental Biology 2023Quote: ... nucleoplasm and chromatin fractions were used for protein analysis with western blotting with the following antibodies were used: anti-DHX9 (Abcam 26271), anti-TBP (Abcam 818) ...
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bioRxiv - Cancer Biology 2022Quote: Protein array analysis was performed using the Human MAPK Phosphorylation Antibody Array (ab211061) according to the manufacturer’s instructions (Abcam, Boston, MA). Visualization was performed with the ChemiDoc Touch Imaging System (Bio-Rad ...
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bioRxiv - Genomics 2023Quote: ... The heterozygous expression of the Dam-Lamin B1 fusion protein was further confirmed by Western blot using an anti-HA antibody at 1 in 5000 dilution (Abcam, ab9110) and an anti-LaminB1 antibody at 1 in 5000 dilution (Abcam ...
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bioRxiv - Neuroscience 2023Quote: ... the cryosections of human brain were mounted on a Polyethylene naphthalate (PEN) Membrane Slide (Zeiss, 415190-9041-000) and then stained with spike protein antibodies using the IHC kit (Abcam, ab210062). After further processing ...
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bioRxiv - Neuroscience 2023Quote: ... CTIP2 (chicken ovalbumin upstream promoter transcription factor-interacting protein) antibody was added (rat, 1:500, Abcam, Cat: ab18465, Lot: GR3272266-13) to facilitate delineation of striatum and incubation continued until the 4 hours were completed ...
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bioRxiv - Evolutionary Biology 2024Quote: ... the included Pierce™ Protein A/G Plus agarose beads were incubated with rabbit anti-myc polyclonal antibody (ab9106, Abcam, USA) and cross-linked using 2.5 mM DSS crosslinker ...
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bioRxiv - Physiology 2020Quote: ... Protein concentrations were measured using BCA Protein Assay Reagent (Abcam, USA). Proteins were separated on SDS-PAGE and transferred to the polyvinylidene fluoride (PVDF ...
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bioRxiv - Cancer Biology 2021Quote: ... Equal amounts of total proteins as determined by BCA Protein Assay were analyzed by SDS-polyacrylamide gel electrophoresis with antibodies specific to IL-8 (Cat. No. 18672, Abcam, Cambridge, MA) and prostate specific antigen (Cat ...
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bioRxiv - Neuroscience 2021Quote: ... slices were then incubated at 4 °C overnight in the primary antibodies: chicken anti-glial fibrillary protein GFAP (targeting astrocytes, 1:200, Abcam #ab4674, USA), goat anti-ionized calcium binding adaptor molecule 1 (Iba1 ...
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bioRxiv - Neuroscience 2021Quote: ... the brain slides were incubated with primary antibody rabbit monoclonal anti-amyloid precursor protein (APP) [clone Y188] (#AB32136, Abcam, USA; 1:500) in blocking solution at 4 °C overnight ...
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bioRxiv - Molecular Biology 2021Quote: ... The membrane was then washed thrice with 1X-TBST and incubated with anti-SARS-CoV-2 N protein antibodies (catalogue No. ab272852, Abcam, 1:1000) in TBST with BSA (2% ...
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bioRxiv - Cell Biology 2021Quote: ... The cells were then fixed with 4% paraformaldehyde and processed for immunofluorescence analysis using GAP SH3 Binding Protein 1 (G3BP1) antibody (green; Abcam, Cambridge, MA) and fluorophore-conjugated secondary antibody ...
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bioRxiv - Animal Behavior and Cognition 2022Quote: ... Astrogliosis was visualized after staining with rabbit Monoclonal Anti-Glial Fibrillary Acidic Protein (GFAP) antibody at a 1:1000 dilution (Abcam, Cambridge, MA). All samples were analyzed on an inverted epifluorescent microscope (Olympus IX71) ...
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bioRxiv - Biochemistry 2021Quote: ... and analyzed by western blot using anti-HBP1 (11746-1-AP, Protein Tech Group, 1:500) or anti-ZMYND19 antibody (ab86555, Abcam, 1:500).
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bioRxiv - Neuroscience 2020Quote: Lysates from DRG neurons were run on 4-12% Bis-Tris gradient gels and proteins were transferred to PVDF membranes that were incubated with antibodies to REST (Abcam, 1:1000), using anti-β-actin as a loading control ...
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bioRxiv - Neuroscience 2019Quote: ... or GT1-7MC4R cells (50 μg of protein) were subjected to immunoprecipitation overnight with 1 μg of a specific α2 AMPK antibody (ab3760, Abcam, Cambridge, MA) coupled with protein G-Sepharose beads (GE Healthcare ...
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bioRxiv - Cancer Biology 2019Quote: ... Whole pancreas protein extracts were isolated and incubated with mouse inflammation/cytokine antibody arrays (40 targets, Abcam, ab133999; 97 targets, Abcam ab169820), according to manufacturer’s instructions ...
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bioRxiv - Pathology 2021Quote: ... tissue proteins (20 μg) were separated by 4-20% SDS-PAGE and immunoblots were developed using antibodies against pSMAD3 (Abcam, 1:1000), SMAD3 (Abcam ...
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bioRxiv - Biochemistry 2019Quote: ... 20 μg of total cell lysates were separated on SDS-PAGE and protein transferred to nitrocellulose membranes that were probed with antibodies against human pancreatic α-amylase (Abcam #ab21156) and ENT2 (Alomone Labs #ANT-052 ...
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bioRxiv - Biochemistry 2021Quote: ... The homogenate was clarified by centrifugation at 21,130 x g for 15 minutes before analysis of protein levels in the supernatant by immunoblotting using antibodies including anti-PKA pRIIα (Abcam, RRID: AB_779040), anti-GFP (Sigma Aldrich ...
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bioRxiv - Immunology 2021Quote: ... Cells were then stained for intracellular spike protein using an Anti-SARS-CoV-2 Spike Glycoprotein S1 antibody (Abcam, ab275759, 1:50) followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor 488 ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... a pair of monoclonal antibodies (clone EPR16588, ab178846; clone EPR16589, ab178847) and a recombinant human protein (ab105593) are available from Abcam (Waltham, MA). Similarly ...