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Citations for abcam :
601 - 650 of 732 citations for 8 Benzylthio 6 oxo octanoic Acid Methyl Ester d4 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... SH-SY5Y and HEK293 cells were determined by modified microculture tetrazolium (MTT) assay (University at Buffalo, State University of New York, NY, USA) or CCK-8 assay (Abcam). Before any treatment ...
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bioRxiv - Bioengineering 2024Quote: ... Primary antibodies against VE-cadherin (1:200, v/v, F-8, Santa Cruz Biotechnology; 1:250, v/v, ab33168, Abcam), vinculin (1 ...
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bioRxiv - Cell Biology 2023Quote: ... GRASP65 was then depleted by treatment with 0.5 mM of the auxin indole-3-acetic acid (IAA, Abcam) for 2 h (Zhang and Seemann ...
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bioRxiv - Immunology 2023Quote: ... The solution was analyzed by the commercially available total bile acid (TBA) assay kit purchased from Abcam (ab239702), following the manufacturer’s instructions.
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bioRxiv - Neuroscience 2024Quote: ... Serum was separated and frozen at −80 before use in either free fatty acid (AbCam cat no. ab65341) or epinephrine (Adrenaline ELISA Kit ...
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bioRxiv - Cell Biology 2022Quote: ... Marker antibodies were detected by Alexa fluor® green rabbit or mouse secondary antibodies (Abcam, supplementary table 6). Co- localization of SaCas9 with desmin or albumin markers in liver tissue was detected by Alexa fluor® secondary antibodies (supplementary table S7) ...
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bioRxiv - Immunology 2021Quote: ... the cells were marked with 4′,6-diamidino-2-phenylindole (DAPI) (cat. n° ab104139, Abcam, Cambridge, MA, USA), and incubated for 5 min ...
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bioRxiv - Cell Biology 2022Quote: ... excised and mounted on glass slides with mounting medium containing 4⍰,6-diamidino-2-phenylindole (DAPI) (Abcam, #104139). Descriptive immunofluorescence images were taken using a Leica SP8 confocal microscope at magnification 20x or 40x ...
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bioRxiv - Physiology 2023Quote: Enzyme- linked immunosorbent assay (ELISA) was used to quantify the concentration of IL-6 (ab100712; Abcam, Waltham, MA) and MCP-1 (ab208979 ...
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bioRxiv - Neuroscience 2022Quote: Stock solutions of 2,3-dioxo-6-nitro-1,2,3,4-tetrahydrobenzo[f]quinoxaline-7-sulfonamide disodium salt (NBQX disodium salt, Abcam; 100 mM) and Tetrodotoxin citrate (TTX ...
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bioRxiv - Developmental Biology 2023Quote: ... Samples were then washed 6 times in PBSGT before incubation with secondary antibodies (ab150063 and ab175745 from Abcam, at 1:500 and 1:250 dilution ...
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bioRxiv - Neuroscience 2023Quote: ... before incubation with anti-syntaxin-6 (1:500 clone C34B2 (Cell Signalling; 2869S) or clone 3D10 (Abcam; Ab12370)) overnight at 4°C ...
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bioRxiv - Cell Biology 2023Quote: ... All samples were stained with 1 mg/mL 4′,6-diamidino-2-phenylindole (DAPI Staining Solution ab228549, Abcam). Cav-1 (ab2910 ...
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bioRxiv - Neuroscience 2024Quote: ... Separate tissue sections were incubated first with rabbit anti-MJFR-14-6-4-2 (1:20000; Abcam, RRID:AB_2714215) or rabbit anti-SOD2 (1:1000 ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Secondary antibodies were incubated for 1-hour followed by 4’-6-diamidino-2-phenylindole (DAPI; 1:1000, Abcam) for 5-minutes ...
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bioRxiv - Neuroscience 2024Quote: ... Slides were washed and mounted with a mounting medium containing 4’,6-diamidino-2-phenylindole (DAPI; ab104139, Abcam). Primary antibodies used were as follows ...
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bioRxiv - Bioengineering 2024Quote: ... and then for 6 h at 4 °C in Alexa fluor 647 labelled goat anti mouse IgG (Abcam) with 1:400 dilution ...
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bioRxiv - Cell Biology 2024Quote: Primary antibodies for immunofluorescence microscopy were: mouse monoclonal anti- alpha tubulin (acetyl K40) [6-11B-1] (Abcam, ab24610), mouse monoclonal anti-SNX1 (BD Transduction Laboratories ...
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bioRxiv - Neuroscience 2022Quote: ... in a heating chamber (37°C) in which the choroid plexus was loaded with Fluo-8 AM (5 μM, 30 min, Abcam, ab142773) with subsequent continuous wash with HEPES-aCSF (~1 ml/min ...
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bioRxiv - Developmental Biology 2022Quote: ... protein was extracted from the 8 cultures using the lysis buffer from the GFP Fluorescent ELISA Kit (ab229403) (Abcam, Cambridge, UK) that was supplemented with Halt Protease Inhibitor Cocktail (Thermo Fisher Scientific ...
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bioRxiv - Bioengineering 2024Quote: ... cells were immuno-stained overnight at 4-8°C with rabbit anti-human IRF1 antibody (1:1000, ab243895, Abcam, Cambridge, UK) or rabbit anti-human pSTAT1 (1:400 ...
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bioRxiv - Molecular Biology 2024Quote: ... Slides were then incubated overnight at 4° C with both α-Cytokeratin-8/18 (1/10, rat monoclonal, University of Iowa, US) and α-Myc antibody (1/200 [Y69], ab32072, rabbit monoclonal, Abcam). After washing with PBS-T ...
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bioRxiv - Neuroscience 2023Quote: ... For double labeling, GFAP antibody was incubated with 8-hydroxyguanosine (8OHG, marker of RNA oxidation) (1:10000, mouse/IgG, Abcam, ab62623), AT100 (1:500 ...
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bioRxiv - Immunology 2024Quote: ... (Cell-Signaling Technology, 2148), Cleaved caspase-3 (CST, 9661S), Cleaved caspase-8 (CST, 8592S), IL-18 (1:1000, WB) (Abcam, ab207323), GSDMC2/GSDMC3 (Abcam ...
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bioRxiv - Genomics 2024Quote: ... 16.7 mM Tris pH 8 and 167 mM NaCl) and incubated overnight with an H3K27ac antibody with rotation at 4 °C (Abcam, ab4729). Protein G Dynabeads (Thermo Scientific ...
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bioRxiv - Immunology 2021Quote: ... Protein concentration in endothelial cell lysates were measured using bicinchoninic acid assay (BCA) Protein Assay Kit (Abcam, Cambridge, UK). NOS production was measured indirectly by Griess reagent in samples using Nitric Oxide Synthase (NOS ...
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bioRxiv - Immunology 2022Quote: ... B16F10 tumor cells were expanded and then supernatant was tested for lactic acid production as per the manufacturer’s instructions (Abcam). Briefly ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Staining was completed using Mayer hematoxylin and 0.2 % eosin (Histolab) or Periodic Acid Schiff (PAS) Stain Kit (Mucin Stain) (Abcam). Imaging of the slides was performed using an Olympus BX60F microscope with an SC50 camera (Olympus ...
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bioRxiv - Neuroscience 2023Quote: ... recovery aCSF included (R,S)-3-(2-Carboxypiperazin-4-yl)propyl-1-phosphonic acid (CPP, 5 μM; Abcam, ab120160) and 2,3-dihydroxy-6-nitro-7-sulfamoyl-benzo[f]quinoxaline (NBQX ...
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Beclin1-Deficient Adipocytes Promote Tumor Progression by YAP/TAZ-dependent Adipocyte TransformationbioRxiv - Biochemistry 2023Quote: The concentration of free fatty acids in the adipocyte-CM was quantified using an ELISA assay kit (ab65341; Abcam). The levels of adipokines in the plasma of WT and BaKO mice were measured using the Mouse Adipokine Array Kit (ARY013 ...
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bioRxiv - Microbiology 2023Quote: ... Prior to DENV-2 infection cells were pre-treated with 1 µM fatty acid synthase (FAS) inhibitor C75 (Abcam) or DMSO diluent only for 24 hours ...
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bioRxiv - Immunology 2024Quote: ... Nucleic acid samples were treated with or without RNase H and then incubated with 5μg of S9.6 antibody (Abcam, USA) or mouse IgG (Beyotime ...
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bioRxiv - Biochemistry 2024Quote: ... and chicken anti-β-galactosidase raised against a 17 amino acid peptide from near the N-terminus (abcam, ab106567). Primary antibodies were detected with HRP-conjugated goat anti-rabbit (abcam ...
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bioRxiv - Cancer Biology 2021Quote: ... Equal amounts of total proteins as determined by BCA Protein Assay were analyzed by SDS-polyacrylamide gel electrophoresis with antibodies specific to IL-8 (Cat. No. 18672, Abcam, Cambridge, MA) and prostate specific antigen (Cat ...
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bioRxiv - Cell Biology 2024Quote: Cells were then incubated at 37°C for 45 min in 90 µl calcium buffer (40 µl for 384-well plates) containing 1 µM Fluo-8 AM (Abcam, cat# 142773). 10x (5x for 384-well plates ...
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bioRxiv - Neuroscience 2023Quote: The accumulation of basal DNA damage was analyzed in 8-week-old patient iPSC-derived neurons by Western Blot for the DNA damage marker γ-H2AX (P-S139, Abcam ab11174). The ability to detect DNA damage was tested in patient iPSC-derived neurons by exposure to 0 ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... the coverslips were incubated overnight (4 °C) with a mouse monoclonal anti-15-LOX-1 antibody (1:100, # ab119774, Lot: GR3322776-8; Abcam, Cambridge, UK), washed repeatedly ...
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bioRxiv - Neuroscience 2024Quote: ... in TBS (pH 8) with 0.05% Tween-20 (TBS-T) and then incubated with the appropriate primary antibody (FTO (mouse, Abcam – ab92821, 1:5000), METTL3 (rabbit ...
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bioRxiv - Cell Biology 2024Quote: ... primary antibody incubation was performed with DNA/RNA damage antibody (8-OHdG; Novus, Littleton, CO, USA) or cleaved caspase-3 (Abcam, Cambridge, UK) (both at 5 µg/mL ...
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bioRxiv - Cancer Biology 2020Quote: ... were incubated for at least 6 hours at 4°C with an antibody against AR (9 μg, Abcam, ab74272) or normal rabbit IgG (Cell Signaling Technology ...
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bioRxiv - Cell Biology 2021Quote: ... Immunohistochemistry was performed on formalin-fixed paraffin embedded lungs following Tris-EDTA pH 9.0 antigen retrieval with an antibody that recognizes CXCL5/6 (Abcam). Routine hematoxylin and eosin (H&E ...
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bioRxiv - Microbiology 2021Quote: ... antibodies for IHC were as follows: acetylated alpha tubulin (cilia marker, clone 6-11B-1, ab24610, Abcam, 1:2000); MUC1-CT (clone MH1 ...
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bioRxiv - Microbiology 2020Quote: ... and 20 mM of L-lysine monohydrochloride in 50 mM Tris/HCl buffer (pH 7.4) were incubated in the presence or absence of ubiquitin-aldehyde (6 nM, Abcam) for 30 min at 37 °C ...
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bioRxiv - Microbiology 2020Quote: ... In a first step the membranes were incubated either with 0.1 μg/mL Anti-6×His® antibody (Abcam) to detect EF-P ...
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bioRxiv - Microbiology 2024Quote: ... In a first step the membranes were incubated either with 0.1 μg/ml anti-6×His® antibody (Abcam). This primary antibody ...
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bioRxiv - Neuroscience 2024Quote: ... IL-6 and IL-10) were measured by Sandwich ELISA according to recommendations of the kit’s manufacturer (Abcam, UK): Mouse IL-6 Matched Antibody Pair Kit (# ab213749) ...
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bioRxiv - Neuroscience 2024Quote: ... for 3 hrs at room temperature on an orbital shaker before being incubated in the primary antibody (1:1,000, conformation-specific anti-αSyn antibody [MJFR-14-6-4-2], Abcam) in 1% non-fat milk in TBST overnight at 4 °C ...
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bioRxiv - Cell Biology 2024Quote: ... samples were rinsed TBS and nuclei were counterstained with 0.5 µg/mL of 4’,6-Diamidino-2-phenylindole (DAPI; Abcam) at RT for 10 minutes ...
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bioRxiv - Cell Biology 2024Quote: ... The remaining chromatin was incubated overnight on a rotator at 4 °C with 6 μl of H3K9me3 (Abcam, ab8898) or H3K27ac (Abcam ...
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bioRxiv - Cell Biology 2024Quote: ... cells were seeded in 6-well plates for 24h prior to 30 minutes incubation with 10µM EdU (Abcam, #ab146186). Cells were then harvested ...