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Citations for abcam :
601 - 650 of 3295 citations for 3 Phenyl 2 thioxo 2 3 dihydro 1H imidazole 4 carboxylic acid since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... anti-claudin 3 (Abcam, ab15102; 1:100 IF, 1:1000 IB); anti-connexin 43 (Abcam ...
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bioRxiv - Bioengineering 2023Quote: ... including rabbit anti-Caspase 3 (1:100; ab184787, Abcam, Cambridge, UK), rabbit anti-Ki67 (1:100 ...
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bioRxiv - Plant Biology 2023Quote: ... rabbit anti-histone 3 polyclonal antibody (Abcam ab1791, 1:5,000 dilution), mouse anti-tubulin antibody (Sigma T5168 ...
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bioRxiv - Immunology 2023Quote: ... Caspase activity was determined with Cleaved Caspase-3 Staining Kit (Abcam) and viability of target cells was assessed using eBioscience Fixable Viability Dye eFluor 780 (ThermoFisher) ...
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bioRxiv - Microbiology 2023Quote: ... and glyceraldehyde 3-phosphate dehydrogenase (GAPDH; 1:5000, Abcam Cat# ab181602); fibroblast-specific protein 1 (FSP1 ...
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bioRxiv - Neuroscience 2024Quote: ... rat anti-complement component 3 (C3) (1:250; Abcam, Cat #: ab11862), goat anti-ionized calcium binding adaptor molecule 1 (Iba-1 ...
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bioRxiv - Cell Biology 2024Quote: ... rabbit polyclonal anti-integrin alpha 3 (1:500, Abcam, Cat# ab190731), rabbit polyclonal anti-integrin alpha 5 (1:250 ...
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bioRxiv - Bioengineering 2024Quote: ... cycle 3 Rabbit CD31 (Abcam, ab182981, dilution 1:200, 1 h), cycle 4 Rabbit CD45 (Abcam ...
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bioRxiv - Bioengineering 2024Quote: ... 1 mM EDTA with 3 uL of anti-Pdh (ab168379, Abcam) and was incubated overnight at 4°C with rotation ...
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bioRxiv - Neuroscience 2021Quote: ... sections were incubated for 48 hours at 4°C on a shaker in primary antibody solution with 2% NHS (1:2000 Rabbit anti-C-FOS, ab208942, Abcam; 1:1000 Rat anti-Ctip2 ...
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bioRxiv - Neuroscience 2021Quote: ... and then incubated overnight at 4°C for 24 hours in 2% normal goat serum together with rabbit anti-Nrf2 at 1:500 (Abcam). Slides were then washed and incubated in the secondary antibody (1:1500 ...
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bioRxiv - Cancer Biology 2020Quote: Cytosol and mitochondria were separated from A375 cells 2 hr or 4 hr after treatment using the Mitochondria/Cytosol Fractionation Kit (Abcam) following the manufacturer’s instructions.
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bioRxiv - Bioengineering 2020Quote: ... blocked spheroids were firstly incubated for 2 days at 4 °C with α-Ki67 (Abcam, stock concentration 0.031 mg/mL) or α-cleaved caspase3 (Cell Signalling Technology ...
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bioRxiv - Evolutionary Biology 2021Quote: ... pCS2-lg-HAtag-WT and pCS2-lg-HAtag-MUT (2 μg) (Supplementary Fig. 4) were then transfected into the HEK293T cell line (ab255449, Abcam) separately ...
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bioRxiv - Genetics 2020Quote: ... Membranes were probed for 1 hour at room temperature and overnight at 4°C with primary antibody (Anti-8-Hydroxy-2’-deoxyguanosine antibody, 1:1000, Abcam) in 3% BSA ...
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bioRxiv - Molecular Biology 2020Quote: ... at room temperature for 2 h and then incubated overnight at 4°C in Blocking Buffer containing primary antibody (anti-mouse PIWIL2/MILI, Abcam Cat# ab36764 ...
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bioRxiv - Immunology 2021Quote: ... Antibody staining was typically conducted on ice for 1 h with rat monoclonal FITC or PE anti-mouse Ly6B.2 (clone 7/4; Abcam), FITC or PE/Cy5 anti-mouse F4/80 (clone BM8 ...
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bioRxiv - Microbiology 2024Quote: Lysates were incubated over night at 4°C with 40 µL of Dynabeads protein G beads conjugated with 2 µg rabbit anti-DDX5 antibody (ab21696; Abcam), or rabbit anti-DDX17 antibody (19910-1-AP ...
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bioRxiv - Cancer Biology 2024Quote: ... secondary antibodies for another 90 minutes at 37°C and mounted with 4’,6-diamidino-2-phenylindole (DAPI) containing mounting medium (Abcam) as published before14 ...
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bioRxiv - Molecular Biology 2023Quote: ... Membranes were blocked with TBST containing 5% skim milk or 5% BSA for 2 hours and incubated overnight at 4°C with the following antibodies: anti-MIF (1:200, ab65869, Abcam), anti-iNOS (1:500 ...
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bioRxiv - Neuroscience 2023Quote: ... Slides were incubated in primary antibody in 0.1 M Tris/2% FBS in a humidified chamber overnight at 4°C (SMI-32, Biolegend 801702, 1:250, SATB2, Abcam ab92446 1:250 ...
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bioRxiv - Genetics 2023Quote: ... Cells were washed three times in PBS before the addition of 4′,6-diamidino-2-phenylindole (DAPI) (Abcam, Cambridge, UK) diluted 1:10,000 in H2O ...
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bioRxiv - Cell Biology 2024Quote: ... All slides then were incubated at 4°C overnight with a single IF stain of ACE-2 (1:100, ab15348, Abcam) or ACE-2 (1:100 ...
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bioRxiv - Cancer Biology 2024Quote: ... Sheared chromatin was diluted to contain 0.16% SDS for antibody binding and incubated overnight at 4 °C with 2 ug anti-H3K27Ac (Abcam, ab4729), anti-H3K4ME3 (Cell Signaling Technology ...
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bioRxiv - Molecular Biology 2024Quote: ... Cells were rotated for 2 hours at 4 °C in 200 μL D-Wash with 1:200 LMNB2 (Abcam, ab8983) followed by a wash step with D-Wash ...
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bioRxiv - Neuroscience 2024Quote: ... Assays were performed using the confor-mation-specific α-synuclein antibody MJF-14 (MJFR-14-6-4-2; Abcam, #ab214033) or the total α-synu-clein antibody syn211 (Abcam ...
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bioRxiv - Neuroscience 2024Quote: ... The membrane was blocked with 5% skimmed milk and probed with MJFR-14-6-4-2 (Abcam, 1:3000 dilution) and EP1536Y (Abcam ...
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bioRxiv - Neuroscience 2024Quote: Dot blots were used to confirm immunoreactivity of generated PFFs with a commonly used conformation-specific anti-αSyn antibody ([MJFR-14-6-4-2], Abcam). 1.5 µl of serially diluted monomer or (un)sonicated PFF samples were loaded onto nitrocellulose membrane (0.45 µm ...
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bioRxiv - Neuroscience 2024Quote: ... brain tissue was quantified by dot blot (described above) using a conformation-specific anti-αSyn antibody ([MJFR-14-6-4-2], Abcam), and diluted PFFs as a standard series ...
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bioRxiv - Neuroscience 2024Quote: ... for 2 hrs at room temperature before incubation with primary antibody (1:500, conformation-specific anti-αSyn antibody [MJFR-14-6-4-2], Abcam) in blocking solution overnight at room temperature ...
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bioRxiv - Cancer Biology 2024Quote: ... 2 mg of protein lysate was incubated overnight at 4°C with 2 µg of MHC-I mouse antibody (ab70328, Abcam) or mouse IgG1 isotype control antibody (5415 ...
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bioRxiv - Evolutionary Biology 2022Quote: ... 0.1% 4-Iodophenylboronic acid (4IBPA) and 0.2 % 5-TAMRA fluorescent dye (Abcam, ab145438) for 30 min ...
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bioRxiv - Molecular Biology 2020Quote: Antibodies (H3K4me1 abcam ab8895, H3K27Ac abcam ab4729, H3K36me abcam ab9050, 2 μg antibody per 2×107 cells) were incubated with 25 μL protein A Dynabeads (Thermo Scientific ...
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bioRxiv - Cancer Biology 2023Quote: ... 50 µg of chromatin was used with anti-Pol II ser 2 phos (2 µg, Abcam ab5095) and anti-Pol II ser 5 phos (2 µg ...
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bioRxiv - Immunology 2020Quote: ... Sections were incubated overnight at 4°C with a rabbit polyclonal anti-mouse blocking antibody (MUC2, ab90007, Abcam Ltd; Occludin, ab168986, Abcam Ltd; caspase 3, ab44976, Abcam Ltd). The sections were washed with PBS and incubated with a secondary antibody for 30 min ...
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bioRxiv - Developmental Biology 2020Quote: ... Slides were washed in PBST as above to remove the excess of the secondary antibody and then one drop of Fluoroshield Mounting Medium with 4’,6-diamidino-2-phenylindole (DAPI) (Abcam, #ab104139) was added on the immunostained samples.
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bioRxiv - Neuroscience 2022Quote: ... then incubated for 2 d at 4°C in primary rabbit anti-TH antibody (Abcam, 1:200, in TBSMT + 5% DMSO). After 6 washes x 1 h with TBST ...
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bioRxiv - Neuroscience 2020Quote: ... Each section was then washed three times in PB for 5 minutes each and incubated for 2 hours at 4°C in a 1:500 dilution of Alexa Fluor 488 goat anti-rabbit secondary antibody (Abcam, Ab150077). From this step on ...
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bioRxiv - Neuroscience 2021Quote: ... Antibodies used were α-syn aggregate-specific antibody MJF-14 (rabbit monoclonal MJFR-14-6-4-2, #ab209538, Abcam, 1:25,000) and mouse monoclonal pS129-α-syn (11A5 ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were blocked and permeabilized with 0.5% TBS-T and 5% BSA and incubated overnight at 4°C with primary antibodies anti-MAP-2 (1:500, Abcam; ab32454) and anti-GFAP (1:500 ...
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bioRxiv - Physiology 2021Quote: ... Membranes were blocked in 3% skim milk for 2 hours and then incubated with primary antibody overnight at 4°C for the following proteins: 4HNE (Abcam, ab46545), β-actin (Santa Cruz Biotech) ...
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bioRxiv - Immunology 2020Quote: ... was used to solubilize N-(1-(2′-(4-Isopropoxyphenoxy)-2,5’-bithiazol-5-yl)ethyl)acetamide (“ACC2 inhibitor 1”; ab142090; purchased from Abcam, Cambridge, UK), 5-[1’-(1-cyclopropyl-4-methoxy-3-methylindole-6-carbonyl)-4-oxospiro[3H-chromene-2,4’-piperidine]-6-yl]pyridine-3-carboxylic acid (“ACC2 inhibitor 2” known as MK-407439 ...
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bioRxiv - Genetics 2022Quote: ... 500 ug lysate was incubated at 4□ overnight with 2 ug of appropriate antibodies (anti-H3K4me3 (Abcam, Cat# ab8580, RRID: AB_306649), anti-H3K36me3 (Abcam ...
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bioRxiv - Bioengineering 2023Quote: ... Hydrogels were blocked with 2% BSA-PBS solution for 1 hr at room temperature and incubated with one or more of the following primary antibodies diluted in 2% BSA-PBS at 4 °C for 48∼72 hr: rabbit anti-Collagen VI (1:200; Abcam, ab182744), rabbit anti-fibronectin (1:200 ...
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bioRxiv - Developmental Biology 2023Quote: ... Sections were blocked in 10% FCS and then incubated overnight at 4°C with the following primary antibodies diluted in 2% FCS: LGALS1 (Abcam ab138513), RBP1 (Invitrogen PA528713) ...
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bioRxiv - Developmental Biology 2024Quote: ... in PBS for 2 hours at room temperature and incubated overnight at 4°C using the following concentrations: anti-H3K9me3 (1:300, Abcam ab8898), anti-H3K27me3 (1:300 ...
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bioRxiv - Biochemistry 2024Quote: ... and then incubated overnight at 4°C with primary antibodies: Anti-beta Amyloid 1-40 [EPR23712-2] (AbCam, Cat. No. ab289991) for Aβ40 and Anti-Amyloid Beta [21F12] (Absolute Antibodies ...
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bioRxiv - Cell Biology 2024Quote: ... The 2 or 4 µm section was used for H&E staining and IHC of anti-human CD11b mAb (abcam #ab307523). The samples were observed by Nikon Eclipse Ti2 microscope carrying NIS-Elements imaging software.
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bioRxiv - Molecular Biology 2021Quote: ChIP Antibodies (H3K4me1 abcam ab8895, H3K27Ac abcam ab4729, H3K36me abcam ab9050, 2 µg antibody per 2×107 cells) were incubated with 25 µL protein A Dynabeads (Thermo Scientific ...
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bioRxiv - Neuroscience 2024Quote: ... Neurons were then immunostained against endogenous AP-2 using Anti-AP-2 complex subunit alpha-1 (Abcam, #ab218624) diluted in blocking solution (1% BSA in PBS ...