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Citations for abcam :
601 - 650 of 3270 citations for 3 4 Ethoxy 3 methoxy phenyl 3 thiophene 2 carbonyl amino propionic acid since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: Competition-EMSA was done as described previously (Jin, et al. 1999) with JEG-3 cell nuclear extract isolated using the EpiSeeker nuclear extraction kit (Abcam). Nuclear extract (3 μg) ...
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bioRxiv - Bioengineering 2024Quote: ... cells were washed 3 times for 5 min with PBS before incubating with a secondary antibody (ab150080 and ab150157, Abcam) for 1 h at room temperature ...
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bioRxiv - Cancer Biology 2024Quote: ... and then mechanically dissociated by repeated pipetting and filtered through a 40-μm nylon mesh prior to adding 3 nM of DRAQ-7 (Abcam) for viability assessment for flow cytometry analysis and/or sorting ...
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bioRxiv - Developmental Biology 2024Quote: ... starting from 3-5x106 cell for TFs and 0.5-1x106 for H3K27ac and using the following antibodies: MEIS1( Abcam ab19867), GATA-6 XP Rabbit mAb (D61E4 ...
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bioRxiv - Physiology 2022Quote: ... the stepwise addition of the uncoupler carbonyl cyanide-p-trifluoromethoxyphenylhydrazone (FCCP) (Abcam) was performed ...
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bioRxiv - Cell Biology 2020Quote: ... diluted 1:33 in PBS 1X supplemented with 3% BSA and with polyclonal rabbit anti-CD31 (cat. Ab28364, Abcam, Cambridge, UK) diluted 1:50 in the same blocking buffer ...
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bioRxiv - Genetics 2021Quote: ... were incubated in blocking solution (0.5% bovine serum albumin in PBS) with 3 μg of antibody to histone-H3 K27ac (Abcam, cat. # ab4729) for 3 h at 4°C ...
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bioRxiv - Immunology 2021Quote: ... Sonicated DNA was pre-cleared using Protein A magnetic beads followed by overnight chromatin immunoprecipitations with 3 ug H3K27Ac (Abcam ab4729), 5 ug FoxP3 (Abcam ab150743) ...
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bioRxiv - Zoology 2020Quote: ... 0-3 days old) and older buds (stage 3, 1 month old) by two different means: anti Phospho-histone H3 (PHH3) immunolocalization (Abcam ab5176) antibody (1:200 ...
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bioRxiv - Immunology 2021Quote: ... Slides were then washed 3×10 minutes in PBS at RT and again blocked with 10% normal donkey serum (Abcam, ab7475) for 20 minutes at room temperature before being incubated with PBS containing WGA-555 (Thermo Fisher ...
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bioRxiv - Bioengineering 2020Quote: ... In the next step the cells were washed with 3% BSA solution and incubated with 1x Phalloidin-iFluor594 (Abcam, stock 1000x) solution (in 1% BSA ...
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bioRxiv - Cancer Biology 2020Quote: Beads that were incubated with FLI 7.3 mouse antibody were resuspended in 100 µL Dig-wash buffer and incubated with rabbit anti-mouse secondary antibody (Abcam, ab46540) at a dilution of 1:100 on a rotator for 1 hour at 4°C ...
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bioRxiv - Neuroscience 2020Quote: ... BLA slices were incubated overnight at 4°C in a solution containing 0.1% Triton X and 3% normal goat serum in PBS and chicken anti-GFP (1:1000, Abcam, ab13970, polyclonal). They were then rinsed in PBS (3 × 10min ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were then washed in PBS (3×10 min) and incubated for 2h in secondary antibody diluted in blocking solution (1:1000 Goat-α-mouse AlexaFluor488, abcam ab150113 ...
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bioRxiv - Immunology 2020Quote: ... Sections were incubated overnight at 4°C with a rabbit polyclonal anti-mouse blocking antibody (MUC2, ab90007, Abcam Ltd; Occludin, ab168986, Abcam Ltd; caspase 3, ab44976, Abcam Ltd). The sections were washed with PBS and incubated with a secondary antibody for 30 min ...
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bioRxiv - Immunology 2021Quote: ... 100 μL of cells expressing mHVEM-mCherry proteins were mixed with 0.3 μg mBTLA- penta-His-tag/mCD160-biotin proteins and 0.5 μg of green fluorescent anti-His-tag (Abcam; Cat: ab1206)/Alexa Fluor 488 conjugated streptavidin (Life technologies ...
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Satb2 acts as a gatekeeper for major developmental transitions during early vertebrate embryogenesisbioRxiv - Developmental Biology 2020Quote: ... Non-specific sites on the membrane were blocked using 3% BSA and further incubated with anti-SATB2 antibody (1:1000 abcam ab34735) in 0.3% BSA overnight at 4°C with constant rocking ...
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bioRxiv - Neuroscience 2022Quote: ... Slices containing VPMpc were incubated overnight at 4°C in a solution containing 0.1% Triton X and 3% normal goat serum in PBS and chicken anti-GFP (1:1000, Abcam, ab13970, polyclonal). They were then rinsed in PBS (3 × 10min ...
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bioRxiv - Neuroscience 2023Quote: ... cells were resuspended in 47 µl of Inside Perm Solution and stained with 3 µl of Anti-PBR antibody [EPR5384] (abcam, ab199836). After 10 minutes of incubation ...
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bioRxiv - Immunology 2023Quote: ... The sections were incubated overnight with anti-cell marker cluster of differentiation (CD) 3 antibody (1:200, Abcam, ab16669, Cambridge, UK) at 4°C in a humidified incubation chamber ...
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bioRxiv - Neuroscience 2023Quote: ... followed by incubation with a rabbit anti-Kv4.3 primary antibody (1:10000, Alomone Labs) together with chicken (G)/mouse (L) anti-TH primary antibody (1:1000, Abcam / Merck) in a carrier solution (1% NGS ...
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bioRxiv - Cancer Biology 2023Quote: ... was performed on sectioned paraffin-embedded tumor constructs or fixed cells cultured for 3 to 7 days on X-tra® slides to analyze biomarker presence of SF-1 (ab240394, Abcam), Melan A (ab210546 ...
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bioRxiv - Biophysics 2024Quote: ... The micro-tissues were then permeabilized in 0.5% Triton X-100 in PBS for 5 min and blocked in 3% bovine serum albumin (BSA) in PBS before they were incubated with primary antibodies (anti-sarcomeric alpha actinin (Abcam #9465) or desmin (Cell Signaling #5332) ...
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bioRxiv - Cell Biology 2024Quote: ... Paraffin embedded liver sections (3 μm) from human and mouse liver samples were stained for aSMA (1:100, Abcam, Cat#ab5694), KI67 (1:50 ...
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bioRxiv - Cell Biology 2023Quote: ... washed twice with 3 % BSA and incubated for 1 h with monoclonal rabbit anti-Ki-67 antibody (Abcam, ab16667, 1:1000) and washed three times with PBS ...
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bioRxiv - Microbiology 2023Quote: 10 µm-thick paraffin sections from juvenile and adult Chinook salmon brains (n=3/group) were deparaffinized and treated with trypsin antigen retrieval solution (Abcam, ab970) at room temperature for 15 minutes ...
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bioRxiv - Cancer Biology 2023Quote: ... The membrane was blocked in TBS containing 3% BSA for 1h at RT and incubated with rabbit anti-B7H3 (1:1000, clone EPNCIR122, Abcam, ab134161) and rabbit anti-GAPDH (1:1000 ...
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bioRxiv - Neuroscience 2022Quote: ... Sections were incubated at room temperature for ~3 hours in secondary antibody solution containing goat anti-rabbit IgG H&L Alexa Fluor 488 (abcam; ab150077).
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bioRxiv - Cell Biology 2022Quote: ... log-phase liquid cultures were divided in two and incubated for different times in YES-Glucose medium treated with 10 µM 3-BrB-PP1 (Abcam, ab143756), or in medium lacking the analog kinase inhibitor ...
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bioRxiv - Biochemistry 2022Quote: Cells were incubated in complete media in the presence of 100 nM bafilomycin A1 (to block the lysosomal turnover of LC3-II) for 3 hrs and subjected to immunoblotting using the mCherry antibody (Abcam, ab125096) with a ratio of 1:1000 ...
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bioRxiv - Biophysics 2023Quote: ... Wells were rinsed with PBS and incubated overnight with 50 μg/mL anti-human CD45RA antibody (Abcam ab212774, lot GR3365431-3), at 4°C.
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bioRxiv - Cancer Biology 2022Quote: ... cells were washed 3 times with PBS for 5 minutes and incubated with AlexaFluor® 647 conjugated secondary antibodies (Ab150079, Ab150115; Abcam) for 1 hour at room temperature ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were blocked in 3% -BSA/1x PBS for 30 min prior to incubation with fluorescence-conjugated Phalloidin (abcam, Cat# ab176753) at 1:000 for 1 hr at room temperature ...
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bioRxiv - Molecular Biology 2024Quote: ... and 30ng of this extract was used for subsequent immunoprecipitation using 4μl of antibody targeting H3K4me3 (Abcam AB8580 lot GR273043-3).
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bioRxiv - Immunology 2024Quote: ... (Cell-Signaling Technology, 2148), Cleaved caspase-3 (CST, 9661S), Cleaved caspase-8 (CST, 8592S), IL-18 (1:1000, WB) (Abcam, ab207323), GSDMC2/GSDMC3 (Abcam ...
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bioRxiv - Microbiology 2024Quote: ... The membrane was washed 3 times in TBST and incubated with the secondary antibody (donkey anti-rabbit IgG HRP conjugate, AbCam 16284) diluted 1/5000 in TBST for 2 h at room temperature ...
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bioRxiv - Immunology 2024Quote: ... Slides were washed 3 x 5 min and stained for 10 min with 1:50 DAB Chromogen in DAB substrate (Abcam ab64238). Slides were rinsed 4x ...
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bioRxiv - Neuroscience 2024Quote: The activity of CatB was examined in the cortex of 3-month-old mice or human fibroblasts using a Cathepsin B Activity Assay Kit (Abcam, #ab65300). The tissue or fibroblasts were homogenised in CB lysis buffer and then incubated on ice for 30 minutes before being centrifuged at 15,000 x g for 5 minutes at 4°C ...
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Varying recombination landscapes between individuals are driven by polymorphic transposable elementsbioRxiv - Genomics 2024Quote: ... We followed the CUT&Tag@home protocol (V.3, (Kaya-Okur et al. 2019; Henikoff et al. 2020)) using H3K9me3 rabbit antibody (Abcam EPR16601) and anti-rabbit Secondary Antibody binding (EpiCypher 13-0047) ...
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bioRxiv - Cancer Biology 2024Quote: ... The assay plate was washed 3 times with 100 µl/well of PBST and 20 µl/well biotinylated detection antibody (Abcam, Ab62352) was diluted (1:100 in dilution buffer ...
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bioRxiv - Genetics 2024Quote: ... washed 3×10 minutes in TBS-T and then blocked for an hour in TBS-T+ (TBS-T with 3% normal goat serum (Abcam, ab7481)) at room temperature ...
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bioRxiv - Genetics 2024Quote: ... The next day the blot was washed 3 times for 5 minutes in PBST before adding 1:500 dilution of anti-Ret antibody (Abcam: ab134100) in PBST and with overnight shaking at 4°C ...
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bioRxiv - Microbiology 2021Quote: ... 5,5’-dithio-bis-(2-nitrobenzoic acid) (DTNB) (Abcam), with the molar extinction coefficient of 14,150 M-1cm-1 at 412nm (72) ...
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bioRxiv - Developmental Biology 2021Quote: ... Non-decalcified E16.5 and P0 samples had mineralized bone visualized by von Kossa staining (n=3 per age and genotype) with Nuclear Fast Red counterstaining as part of an established protocol (Abcam, Cambridge, UK). For osteoclast staining ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were then incubated for 60 min at room temperature with primary antibody dilutions prepared in 3 % BSA in PBS as follows: mouse anti-Bub1 (ab54893, Abcam, 1:500); mouse anti-Mad2 (sc-65492 ...
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bioRxiv - Bioengineering 2020Quote: ... Permeabilized cells were rinsed 3 times with 1X PBS and incubated in 1X PBS with primary antibody Rabbit-α-Tomm20 (Abcam, ab186735) and Mouse-α-LaminA/C (Abcam ...
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bioRxiv - Neuroscience 2020Quote: ... the samples were incubated for 3 h at room temperature with the goat anti-chicken antibody conjugated with Alexa Fluor® 488 (Abcam 150169) at a concentration of 1:500 in PBS-blocking buffer ...
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bioRxiv - Neuroscience 2020Quote: ... every fourth section throughout the hippocampus/dentate gyrus was collected and stained with panel 3 = anti-mouse BrdU (ab8152, Abcam, 1:500), anti-mouse doublecortin (DCX ...
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Role of autophagy in sepsis-induced skeletal muscle dysfunction, whole-body metabolism, and survivalbioRxiv - Cell Biology 2021Quote: ... Membranes were blocked in PBS + 1% Tween® 20 + 5% BSA for 1 h at room temperature then incubated overnight with specific primary antibodies at 4°C.Membranes were washed in PBST (3×5 min) then incubated with HRP-conjugated goat anti-rabbit (ab6721) or rabbit anti-mouse (ab6728) secondary antibodies (Abcam, Cambridge, MA) for 1 h at room temperature ...
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bioRxiv - Cell Biology 2022Quote: ... The cultures subsequently underwent 3 cycles of 10 min PBS washes and incubated for 40 min with the appropriate secondary antibodies (Abcam or Jackson) and DAPI ...