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Citations for abcam :
6351 - 6400 of 6498 citations for Mouse CXCL9 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... Serine racemase treatment of human serum was performed with the addition of 5 µl of proprietary serine racemase solution from a DL-serine assay kit (Abcam) to 1 ml of serum for 3 h ...
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bioRxiv - Neuroscience 2024Quote: ... GDH activities in primary astrocytes were determined with glutamate dehydrogenase activity assay kit following with the manufactureŕs instructions (Abcam, ab102527).
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bioRxiv - Molecular Biology 2024Quote: ... then resuspended at 1×105 cells/mL in ice-cold PBS and analysed using the comet assay kit (Abcam, #ab238544) according to manufacturer’s instructions with gel electrophoresis run for 20 minutes at 3 volts/cm.
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bioRxiv - Microbiology 2024Quote: The SOD activity of the gastrocnemius muscle tissues from the mice was assessed using the colorimetric assay kit (Abcam, ab65354) as per the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2019Quote: The TC and free cholesterol content of hLECs were measured colorimetrically using a Cholesterol assay kit according to the manufacturer’s protocol (#ab65359; Abcam, Cambridge, UK). Harvest the amount of hLECs necessary for each assay (initial recommend = 1 x 107 cells) ...
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bioRxiv - Developmental Biology 2021Quote: ... slides were incubated with secondary antibody for 2 h at room temperature before DAB reaction using the DAB detection kit (Abcam #ab64238). Slides were counterstained with hematoxylin to stain nuclei ...
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bioRxiv - Cell Biology 2022Quote: ... NAD+/NADH ratio and the concentration of total NAD+ and NADH was determined by NAD/NADH fluorometric assay kit from Abcam (ab176723) following manufacture’s protocol ...
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bioRxiv - Immunology 2021Quote: ... Staining was completed per manufacturer’s instructions for RNAScope® multiplex V2 detection kit (ACD Bio) coupled with immunofluorescent protein staining for either Cytokeratin19 (ab52625 Abcam) at 1:250 dilution ...
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bioRxiv - Neuroscience 2020Quote: ... Oil Red O (ORO) staining was performed on muscle cryosections (10μm) to visualize neutral lipids using an ORO kit (Abcam, Cambridge, GBR). Briefly ...
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bioRxiv - Bioengineering 2020Quote: Quantification of proteins via C-terminal GFP tags was performed using a GFP quantification kit from AbCam (Cambridge, UK, product # ab171581) according to manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: ... The general activity of MMPs in iPSC-astrocyte supernatants was determined using the MMP Activity Assay Kit (Abcam, Cat. no.: ab112147) following the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2020Quote: The number of apoptotic cells before and 1h and 4 weeks after music exposure (n=10 in each group) was evaluated with the TUNEL method using the “17- 141 TUNEL Apoptosis Detection Kit” (Abcam, UK) in accordance with the standard protocol provided by the manufacturer and analyzed by confocal microscopy (Olympus FLUOVIEW FV10i-W ...
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bioRxiv - Cancer Biology 2020Quote: ... ChIP for RNA polymerase II was performed according the iDeal ChIP-Seq Kit instructions using the anti-Pol2 antibody (Abcam, ab5408). Immunoprecipitated DNA was bisulfite converted using the EZ DNA Methylation Gold Kit (Zymo) ...
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bioRxiv - Bioengineering 2021Quote: ... Twenty microliters of reagent was added directly to each well plate following the instructions of the MTS Cell Proliferation Assay Kit (colorimetric ab197010, abcam®). The culture plate was incubated for 3 h at 37°C in a humidified atmosphere containing 5% CO2 ...
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bioRxiv - Plant Biology 2021Quote: ... 1 mM dithiothreitol (DTT) and 1% (v/v) protease inhibitor cocktail and then used for protein quantification with BCA protein quantitative Kit (Abcam ab6789). The protein sample (200μg amounts ...
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bioRxiv - Molecular Biology 2021Quote: The activity of mitochondria Complex I was measured in peeled murine esophageal epithelium using Complex I Enzyme Activity Assay kit (Abcam, ab109721) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: ... cells were incubated with filipin III (1:100) in PBS for 2 h as described in the manufacturer’s protocol of the cholesterol assay kit (Abcam Biochemicals, ab133116). Filipin III was also added in all following incubation steps with the primary and secondary antibody to enable proper staining ...
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bioRxiv - Cell Biology 2021Quote: ... The fixed cells were washed three times with water and then partially lysed with a yeast nuclei isolation kit (Abcam 206997) using the following modified protocol ...
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bioRxiv - Cancer Biology 2022Quote: ... Peroxidase and non-specific protein blocking were done as per the instructions using the Abcam ABC detection kit (ab64264) and incubated with the following primary antibody dilutions: anti-Androgen Receptor (Abcam, ab74272), anti-Ki67 (Abcam ...
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bioRxiv - Microbiology 2022Quote: ... solani IPO 2222 WT strain and ΦD5□resistant Tn5 mutants were isolated using a Lipopolysaccharide Extraction Kit (Abcam, Symbios, Gdansk, Poland) with a modified protocol (Bartnik et al. ...
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bioRxiv - Immunology 2022Quote: ... Alanine liberated was measured as increasing fluorescence signal (Resorufin, Ex/Em: 535/587 nm) recorded at 25 °C using an L-alanine assay kit (Abcam, ab83394) at 25 °C according to manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... and CTLA-4 Ig (Abatacept) were generated using the APC Conjugation Kit - Lightning-Link® according to manufacturer’s instructions (Abcam, ab201807). APC conjugated anti-PD-L1 antibodies were procured from Biolegend (clones MIH1 ...
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bioRxiv - Cancer Biology 2020Quote: Mammary glands were dissected and homogenized in NOS assay buffer and further processed following the Nitric Oxide Synthase Activity Assay kit (Abcam, ab211083) protocol for measuring enzymatic activity of nitric oxide synthase (NOS) ...
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bioRxiv - Cancer Biology 2019Quote: ... Nuclear protein sample extract from washed cell pellets was carried out according to the manufactures protocol using cell fraction kit – standard (Abcam, ab109719). Nuclear pellets were resuspended in 100mM ammonium bicarbonate and lysed by sonication at 26 joules ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... and deparaffinized in serial baths of xylene and ethanol followed by staining using Mayer hematoxylin and 0.2% eosin (Histolab Products AB, Askim, Sweden) or picrosirius red staining kit (Abcam, Cambridge, UK). The stained slides were imaged using an Aperio CS2 image capture device.
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bioRxiv - Cancer Biology 2020Quote: ... Cells were incubated with CM for 24 or 48h after which cells were stained to detect DNA fragmentation in apoptosis using the BrdU-Red DNA (TUNEL) kit (Abcam, ab66110) following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2019Quote: A luminescent ATP detection assay kit was used to determine the total levels of cellular ATP as per manufacturer’s instructions (abcam, Cat #ab113849). Briefly ...
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bioRxiv - Biochemistry 2021Quote: Histological analyses of the NAFLD tissues were performed using the H&E Stain Kit (Hematoxylin and Eosin) (ab245880; Abcam, Cambridge, UK) and the Oil Red O Stain Kit (Lipid Stain ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... and deparaffinized in serial baths of xylene and ethanol followed by staining using Mayer hematoxylin and 0.2% eosin (Histolab) or picrosirius red staining kit (Abcam, Cambridge, UK). The stained slides were imaged using an Olympus BX60F microscope with an SC50 camera.
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bioRxiv - Cell Biology 2021Quote: The relative contents of endogenous sphingomyelins in WT and SIRT1 KO mESCs were analyzed using a commercially available Sphingomyelin Assay Kit (Abcam ab133118) per manufacturer’s instruction.
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bioRxiv - Cancer Biology 2021Quote: ... cells were collected by trypsinisation and Annexin V staining was carried out using an Annexin V-iFluor 647 Apoptosis Detection Kit (Abcam, ab219919), following manufac-turer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... Sorted satellite cells were treated with UV radiation at different dosages and the comet assay was operated immediately after irradiation through a Comet Assay Kit (Abcam, ab238544) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: Grown BEC-organoids were treated with the FA-mix for 4 days and triglyceride levels were measured with a Triglyceride kit (Abcam, ab65336) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... Monoclonal α-GPEET and α-EP IgG were conjugated with Allophycocyanin (APC) fluorescence molecule by APC Conjugation Kit – Lightning Link (Abcam). Cells were stained with α-GPEET or α-EP or both α-GPEET and α-EP to detect the loss of GPEET and EP as the indicator of the presence of non-PCF cells in the induced culture ...
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bioRxiv - Genetics 2022Quote: ... The mixture was centrifuged at 10000g for 5 min and 5μl of the supernatant was used to measure fumarate levels per instruction from the commercial fumarate kit (Abcam, Cambridge, UK).
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bioRxiv - Physiology 2022Quote: RalGDS RBD Agarose beads were used to selectively pull-down the active form of Rap1 (Rap-GTP) from isolated cardiac mitochondria (Rap1 Activation Assay Kit, Abcam, ab212011). Samples of mitochondria ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were incubated for 1 hour at room temperature with PSR staining solution before rinsing twice in Acetic Acid solution (Picrosirius Red Stain Kit, Abcam, ab150681) and imaging ...
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bioRxiv - Systems Biology 2024Quote: ... The detection of EdU incorporation was performed according to the protocol provided by the EdU proliferation kit (Abcam, ab219801, iFluor 488). In brief ...
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bioRxiv - Cell Biology 2024Quote: The mitochondrial membrane potential (ΔΨm) was measured with the JC-1 mitochondrial membrane potential assay kit (ab113580, Abcam, Cambridge, United Kingdom) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... The supernatant was vacuum dried and the dried lipids were dissolved in 200 μL of the assay buffer supplied by the Cholesterol/ Cholesteryl Ester Assay Kit (Abcam, ab65359). 5 μL of each sample was used for each reaction.
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bioRxiv - Neuroscience 2024Quote: ... Sections measuring 5 μm were then cut using the Leica RM2165 and subsequently stained with Movat’s Pentachrome staining kit (Abcam Cat# ab245884). For H&E staining analysis ...
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bioRxiv - Cancer Biology 2024Quote: ... ROS indicator dye was prepared according to the manufacturer’s protocol using DCFDA/H2DCFDA - Cellular ROS Assay Kit (Abcam, ab113851, Cambridge, UK), and was added to each well ...
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bioRxiv - Neuroscience 2024Quote: ... Cresyl Violet & Luxol Blue staining of brain sections was performed using a commercially available kit according to the protocol provided (Abcam, ab150675). Brain images were taken on a Zeiss Axio Scan Z1 slide scanner.
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bioRxiv - Immunology 2024Quote: ... was performed by flow cytometry and triglyceride release assay was determined in supernatant after low-speed centrifugation (300 g, 10 min) using Triglyceride Assay kit (Abcam ab65336). GMPs were collected in a supernatant of granulocytes (3-5 x 106/ml ...
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bioRxiv - Genetics 2023Quote: ... slides were stained for goblet cells and mucins using an Alcian Blue dye and Nuclear Red Fast counterstain kit (Abcam; ab150662). Quantification of the percent area of Alcian blue staining ...
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bioRxiv - Microbiology 2023Quote: ... The preparation of samples and lactate level measurement procedure were followed as specified in the Lactate Assay Kit (Colorimetric) (ABCAM - ab65331) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: Detection of cytoplasmatic and mitochondrial ROS levels was performed by means of a commercial DCFDA / H2DCFDA - Cellular ROS Assay Kit (abcam, ab113851) and MitoSOX™ Red Mitochondrial Superoxide Indicator (Invitrogen ...
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bioRxiv - Cancer Biology 2023Quote: ... Samples were pre-cleared using the beads provided in the kit and incubated overnight at 4°C with 5 µg of commercially available antibodies against lamin A/C (Abcam, #ab108595), phospho-Ser301 lamin A/C (Affinity ...
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bioRxiv - Cancer Biology 2023Quote: ... Samples were diluted 1:400 in Milli-Q water and lactate concentration was measured using a Lactate assay kit (Abcam, ab65330) according to the manufacturer’s protocol (using 1.5 µL of Enzyme Mix per well) ...
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bioRxiv - Neuroscience 2023Quote: ... and cathepsin D (CatD) endo-peptidase activity was determined using commercial assay kits (Cathepsin B: ab65300, Abcam; Cathepsin D: ab65302, Abcam). Samples were homogenized in Lysis buffer as per the manufacturer’s instructions ...