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Citations for abcam :
6301 - 6350 of 7426 citations for Mouse Protein Fantom RPGRIP1L ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... 0.4% Triton X) before being stained overnight at 4C with the following primary antibodies: mouse anti-APC (CC1) (Abcam #ab16794, 1:100), goat anti-CCL11 (R&DSystems #AF-420-SP ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were washed twice with PBS before incubation overnight at 4°C in primary mouse IgG2b anti-NeuN (1:500, Abcam cat# ab104224). Sections were washed twice the following day with 1X PBS followed by incubation in goat anti-IgG2b AlexaFlour 647 (1:1000 ...
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bioRxiv - Biochemistry 2024Quote: ... Immunoprecipitation of mGluR2 was performed overnight at 4°C using a mouse monoclonal anti-mGluR2 N-terminal antibody (Abcam, Catalog no. ab15672), followed by RNA isolation ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... the coverslips were incubated overnight (4 °C) with a mouse monoclonal anti-15-LOX-1 antibody (1:100, # ab119774, Lot: GR3322776-8; Abcam, Cambridge, UK), washed repeatedly ...
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bioRxiv - Plant Biology 2024Quote: ... using a solution containing goat polyclonal secondary antibody to mouse or rabbit IgG – H&L conjugated to Alexa Fluor 488 (Abcam AB150113, AB150077), added in a 1:100 dilution ...
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bioRxiv - Molecular Biology 2024Quote: ... washed three times with PBS for and subsequently stained with AF647 anti-mouse secondary antibody (1:200, Abcam, ab169348 in 1% BSA) for 1 h at RT ...
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bioRxiv - Cell Biology 2020Quote: ... Co-immunoprecipitation of α-tubulin interacting proteins was performed from total cell lysates using α-Tubulin specific antibodies (rabbit anti-α-tubulin, ab52899, Abcam) and Protein A/G Sepharose beads (Santa Cruz Biotechnology) ...
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bioRxiv - Cell Biology 2020Quote: ... The eluates were boiled with LDS protein loading buffer (Thermo, NP0008) and loaded on NuPAGE for gel electrophoresis and Western blotting with antibody to ANT1 (Abcam, ab102032, 1:3000). In some experiments ...
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bioRxiv - Genetics 2021Quote: ... ran side by side in a Criterion Precast Gradient 4-15% SDS-PAGE gel for western blotting probing of key intracellular organellar markers and proteins of interest: HSP60 (Abcam, cat. number ab46798), Calnexin (Abcam ...
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bioRxiv - Neuroscience 2020Quote: ... Proteins were transferred to 0.2 um nitrocellulose or PVDF membranes and detected with primary antibodies targeting LRRK2 (ab133474, Abcam, RRID: AB_2713963, 1:500), pS935 LRRK2 (ab133450 ...
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bioRxiv - Plant Biology 2021Quote: ... For each ChIP/re-ChIP assay, 20 μg DNA/protein complexes were immunoprecipitated with anti-H3K27me3 (Milipore, Cat No 07-449) and anti-H3K18ac (Abcam, Cat No ab1191) antibodies using the Re-ChIP-IT kit (Active Motif ...
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bioRxiv - Immunology 2021Quote: ... SARS-CoV-2 infected cells were visualized using SARS-CoV-2 S protein specific immunochemistry staining with anti-SARS-CoV-2 spike glycoprotein S1 antibody (Abcam, Cambridge, Great Britain) as described previously [64] ...
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bioRxiv - Neuroscience 2021Quote: ... Co-labeling for astrocytes was performed using the same anti-glial fibrillary acidic protein antibody described above (GFAP; Abcam, ab53554; 1:250 dilution). Secondary antibody incubation labeling GFAP positive cells was performed for 1 hour at room temperature using Alexa Fluor 488-conjugated Donkey anti-Goat (ThermoFisher ...
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bioRxiv - Physiology 2021Quote: ... A commercially available monoclonal antibody was used to detect electron transport chain proteins (rodent OXPHOS Cocktail, ab110413; Abcam, Cambridge, UK, 1:250 dilution), including V-ATP5A (55kDa) ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Membranes were then probed to determine protein levels using the following antibodies diluted in 5% non-fat milk in 1x TBST: matrix metalloproteinase 9 (MMP9) (Abcam ab38898, 1:1000) and MMP2 (Abcam ab92536 ...
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bioRxiv - Genetics 2019Quote: ... Proteins were transferred to a polyvinylidene difluoride membrane and probed with well-characterized monoclonal antibody of anti-Fibrillin 1 (Abcam ab124334, Cambridge, UK).
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bioRxiv - Bioengineering 2019Quote: ... Tangential cortical sections of 30 μm-thickness were sliced using a cryostat and directly mounted on gelatin-subbed glass slides for immunohistological staining of GFAP (glial fibrillary acidic protein, Abcam, Cambridge, MA, USA) for astrocytes ...
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bioRxiv - Microbiology 2020Quote: Paraffin embedded tissue sections were stained for SARS-CoV-2 nucleocapsid protein (1:500) and ionized calcium binding adaptor molecule 1 (Abcam; ab5076; 1:50) using a Leica Bond RXm automated staining instrument following permeabilization using 0.01% Triton X diluted in Trisbuffered saline (TBS) ...
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bioRxiv - Plant Biology 2024Quote: ... Protein expression of rSPL9-GFP was verified by SDS-PAGE and immunoblotting using an anti-GFP antibody (Abcam, Cat# ab290, 1:10,000 dilution) and HRP-conjugated secondary antibody (Promega ...
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bioRxiv - Physiology 2024Quote: ... A commercially available monoclonal antibody was used to detect electron transport chain proteins (rodent OXPHOS Cocktail, ab110413; Abcam, Cambridge, UK, 1:250 dilution), including V-ATP5A (55kDa) ...
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Mucociliary Clearance Augmenting Drugs Block SARS-Cov-2 Replication in Human Airway Epithelial CellsbioRxiv - Microbiology 2023Quote: ... while sections of caspase and protein S staining were incubated with goat anti-rabbit IgG secondary antibody conjugated with HRP (Abcam ab6721, 1:1,000). ImmPACT DAB Peroxidase (HRP ...
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bioRxiv - Developmental Biology 2023Quote: Expression of Br-Z1 and Blimp-1 proteins was inferred from staining knock-in strains with chicken anti-GFP (Abcam Ab13970 1:500) and an anti-chicken secondary antibody (Invitrogen A-21437 1:1000).
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bioRxiv - Microbiology 2023Quote: ... Primary antibody incubation was conducted for 3 hours at room temperature (1:2,000 α-N protein, Sino Biological 40143-R001; 1:500 α-nsp3, abcam ab283958) followed by a 1 hour ...
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bioRxiv - Pathology 2024Quote: ... IHC protocol modified from a previously described protocol(37, 38) using a rabbit anti-SARS-CoV-2 S protein antibody (Abcam, ab272504, Cambridge, UK) at 1:5000 dilution (0.2 µg/mL) ...
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bioRxiv - Cell Biology 2024Quote: ... Transfer quality and total protein were assessed by Ponceau-S staining before the membranes were probed for Myc-tag (Abcam, #ab9106, 1:1000). Fluorophore-conjugated secondary antibodies were used for detection using the Licor Odyssey CLx system (anti-rabbit IgG ...
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bioRxiv - Biophysics 2021Quote: ... the cells were blocked for 1 h at room temperature using an unconjugated goat anti-mouse IgG H&L antibody (polyclonal, 1:1000, Abcam ab6708, Cambridge, UK) diluted in previously mentioned blocking solution ...
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bioRxiv - Biophysics 2021Quote: ... the samples were incubated with the primary antibody (monoclonal mouse anti-vinculin, 1:100, Sigma V9264, St. Louis, USA; polyclonal rabbit anti-talin 1, 1:100, Abcam ab71333, Cambridge, UK) diluted in blocking solution for 1.5 h at room temperature or overnight at 4 °C.
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bioRxiv - Cell Biology 2020Quote: ... the primary rabbit mono-specific antibodies were diluted into a concentration of 2-4 µg/ml in blocking buffer (PBS + 4% FBS) containing 1 µg/ml mouse anti-alpha-tubulin (Abcam, ab7291, Cambridge, UK) and chicken anti-KDEL respectively (Abcam ...
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bioRxiv - Neuroscience 2021Quote: ... This was directly followed by incubation overnight at room temperature with primary antibodies (GFAP; host: mouse; diluted 1:1000; Sigma-Aldrich; Cat# MAB360. GFP; host: chicken; diluted 1:2000; Abcam; cat# ab13970) diluted in antibody solution (ABS ...
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bioRxiv - Molecular Biology 2020Quote: ... Bottom halves of dermal sample membranes were incubated with rabbit anti-TIMP3 (Cell Signalling Technology; D74B10) and top halves with mouse anti-HSA (Abcam, ab10241; loading control). Bottom half of epidermal sample membranes were incubated with rabbit anti-RPL36 (Sigma ...
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bioRxiv - Neuroscience 2019Quote: ... Slices were washed 3 times and then stained simultaneously with two secondary antibodies: goat anti-mouse IgG H&L (Alexa Fluor® 488) preadsorbed (1:400 diluition; ab150117; Abcam, Cambridge, UK) and rhodamine red -X-coniugated affinity pure goat anti-rabbit IgG (H+L ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... the samples were incubated with Alexa Fluor 488- or 594-conjugated goat-anti-mouse or goat-anti-rabbit secondary antibodies (Abcam, 1:500 dilution) overnight at 4 °C and rinsed several times in PBST before mounting in ProLong Diamond Antifade mountant (Invitrogen ...
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bioRxiv - Neuroscience 2020Quote: ... Afterward the sections were incubated with 1:100 diluted mouse polyclonal anti-GFAP (Cusabio, Houston, TX, USA) and 1:500 diluted rabbit polyclonal anti-HuC/D (Abcam, Cambridge, United Kingdom) overnight at 4°C· followed by incubation with Cy3-labeled goat anti-mouse IgG (Beyotime ...
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bioRxiv - Cell Biology 2021Quote: ... and for >16 hrs at 4°C with the following primary antibodies: mouse-α-CD63 (1:1000, clone TS63; Abcam, Cambridge, United Kingdom), mouse-α-Flotillin-1 (1:1000 ...
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bioRxiv - Neuroscience 2020Quote: ... so membranes were also incubated with a mouse monoclonal anti-beta actin primary antibody (dilution: 1:5000, product number: ab8226, Abcam, Cambridge, MA, USA). After TBST washing three times for 10 minutes each wash ...
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bioRxiv - Neuroscience 2022Quote: ... rabbit anti-CaVβ3 (Alomone Labs #AAC-008, 1:800) or mouse anti-CaVβ4 (Neuromab #75-054, 1:1000) and rabbit anti-β-tubulin (Abcam #ab6046, 1:600,000). Membranes were then incubated in LiCor Blocking Buffer as described above and LiCor IRDye secondary antibodies ...
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bioRxiv - Physiology 2022Quote: Both neonatal and adult ducts were stained using antibodies against the cholangiocyte marker keratin 19 (K19, 1:10, Developmental Studies Hybridoma Bank, TROMAIII) and for the myofibroblast marker α-smooth muscle actin (mouse α-SMA, Abcam ab7817). Adult bile ducts were also stained for hematoxylin and eosin and Masson trichrome.
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bioRxiv - Neuroscience 2022Quote: ... FoxP2 (mouse, Merck Millipore: MABE415, RRID: AB_2721039; 1:2000; Invitrogen: MA5-31419, RRID: AB_2787055; 1:2000; rabbit, Abcam: ab16046, RRID: AB_2107107; 1:500), Calb1 (rabbit ...
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bioRxiv - Immunology 2022Quote: ... PVDF membrane was blocked with 5% skim milk buffer and then incubated with one of the following primary antibodies: mouse anti-HSP70 (ab2787, Abcam, Cambridge, MA, USA), rabbit anti-TSG101 (14497-1-AP ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were then incubated for 60 min with primary antibodies diluted in blocking solution as follows: mouse anti-Pyruvate dehydrogenase E2/E3 (PDHE2/E3, ab110333, Abcam, 1 μgmL−1); mouse anti-clathrin heavy chain (X22 ...
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bioRxiv - Cell Biology 2022Quote: ... We used mouse anti-beta-tubulin (E7, Developmental Studies Hybridoma Bank, 1:300 dilution) and rabbit anti-histone H3 (Abcam, 1:500 dilution). Embryos were then washed 4 x 2 h in PBT and incubated overnight at 4°C in PBT supplemented with goat anti-mouse and goat anti-rabbit secondary antibodies coupled to Alexa Fluor 488 and 568 (Invitrogen) ...
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bioRxiv - Molecular Biology 2020Quote: ... in a Tris-glycine-methanol transfer buffer overnight and detected with the following antibodies: 1:5000 monoclonal mouse anti-Hsp70 [C92F3A-5] (Abcam Cat# ab47455, RRID:AB_881520) (abcam) ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... e cells were then incubated with primary antibodies against mouse 8-hydroxy-2’-deoxyguanosine (8-OHdG) (1:200)(ab48508, 1:200 dilution; Abcam, Cambridge, United Kingdom) at 4°C overnight ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... For western blot analysis, a human polyclonal IgG anti-SARS-CoV-2 (CIGB, Cuba) and a mouse monoclonal antibody against CK2α (Abcam, Cambridge, United Kingdom) were used as primary antibodies ...
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bioRxiv - Cancer Biology 2020Quote: ... Primary antibodies used and dilution ratios are: mouse monoclonal anti-Acetylated α-Tubulin (1:500, ab24610, clone 6-11B-1, abcam, Cambridge, MA), mouse monoclonal anti-Acetylated α-Tubulin (1:500 ...
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bioRxiv - Neuroscience 2021Quote: ... stained and incubated in a humidified chamber with rabbit anti-mouse Collagen I overnight at 4°C (1:100 dilution Abcam Catalog No. ab34710); washed 3 times in TBS-Tween (.05% Tween ...
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bioRxiv - Neuroscience 2022Quote: ... for 1 h at room temperature and were incubated overnight at 4 °C with primary rabbit polyclonal antibodies against mouse MAGL (Abcam, Cambridge, UK; ab180016) or human MAGL (LSBio ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were washed and incubated with the appropriate fluorescein-conjugated secondary antibodies (Alexa Fluor 594-labeled goat anti-mouse IgG from Abcam for γ-H2AX; Alexa Fluor 488-labeled goat anti-rabbit IgG from Abcam for RAD51). After washing ...
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bioRxiv - Immunology 2019Quote: ... The tissue was embedded in paraffin and sections were stained with an antibody against RELM-α (polyclonal rabbit anti-mouse, Abcam, Cambridge, UK). Nuclei were stained with haematoxylin (Merck) ...
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bioRxiv - Neuroscience 2020Quote: ... and incubated for 2h in secondary antibody diluted in blocking solution (1:1000 Goat-α-mouse AlexaFluor488, abcam ab150113; 1:1000 Goat-α-rabbit AlexaFluor555, abcam ab150090) and washed again in PBS (4×10 min) ...