4 2 Hydroxy 1 Methoxyethyl 1 2 Benzenediol citations for abcam

Quote: ... in PBS-Tween (1:250, overnight, 4°C) and FITC anti-mouse alpha muscle actin (Abcam, ab8211-500, 1:100, overnight, 4°C). Tissues were washed with PBS-Tween before incubation with secondary antibody PE-Goat anti-Rabbit IgG (Abcam ...

Identifying challenges and opportunities for histone peptides in protein engineering platforms

Jenna N Meanor, Albert J Keung, Balaji M Rao, bioRxiv - Bioengineering 2021

Quote: ... or H3K9me3 antibody for 30 minutes with rotation at 4°C in 100 μL of incubation buffer (ab1220 1:200, ab9045 1:200, ab8898 1:250, Abcam). Incubation buffers tested were 0.1% PBSA ...

Neural circuit analysis using a novel intersectional split intein-mediated split-Cre recombinase system

Audrey Tze Ting Khoo, et al., bioRxiv - Neuroscience 2020

Quote: ... the same solution containing the primary antibody overnight at 4°C (anti-tRFP, 1:1000, Evrogen; anti-GABA, 1:2000, MilliporeSigma anti-parvalbumin, 1:2000, Abcam; anti-somatostatin ...

The chemical chaperone 4-phenylbutyric acid rescues molecular cell defects of COL3A1 mutations that cause vascular Ehlers Danlos Syndrome

Ramla Omar, et al., bioRxiv - Genetics 2024

Quote: ... membranes were incubated with primary antibody overnight at 4°C (BIP [1:40,000 BD Transduction 610979], ATF4 [1:1000, Santa Cruz Biotechnology sc-200], ATF6 [1:1000 Abcam ab122897] ...

Gallic Acid, a Methyl 3,4-Dihydroxybenzoate Derivative, Induces Neural Stem Cells to Differentiate and Proliferate

Junxing Jiang, et al., bioRxiv - Neuroscience 2021

Quote: ... the cells were incubated with the primary antibodies of interested proteins at 4°C overnight (Nestin, 1:1000, Sigma; Tuj1, 1:1000, Sigma; GFAP, 1:1000, Abcam; Ki67, 1:1000, ProteinTech). The next day ...

Abelson-induced phosphorylation of TACC3 modulates its interaction with microtubules and affects its impact on axon outgrowth and guidance

Burcu Erdogan, et al., bioRxiv - Cell Biology 2020

Quote: ... and labelled with primary antibody (1:1000 diluted in blocking buffer made up by 1% non-fat dry milk in calcium and magnesium free PBS) to tyrosinated tubulin [YL1/2] (rat monoclonal, ab6160, Abcam) for 45 min at room temperature which is followed by PBS washes repeated three times and 10 min of blocking ...

Circulating levels of epirubicin cause endothelial senescence while compromising metabolic activity and vascular function

Amanda J Eakin, et al., bioRxiv - Cell Biology 2020

Quote: ... Slides were washed 3 times in PBS and then incubated for 2 h in secondary antibody (1:200 dilution AlexaFluor647 conjugated donkey anti-goat (ab150135, Abcam) and 1:200 dilution FITC-conjugated rabbit anti-rat (ab6730 ...

The secretome of M1 and M2 microglia differently regulate proliferation, differentiation and survival of adult neural stem/progenitor cell

Xue Jiang, Saini Yi, Qin Liu, Jinqiang Zhang, bioRxiv - Neuroscience 2021

Quote: ... Then the cells were blocked in 10% donkey serum for 2 h and incubated with primary antibodies (goat anti-Iba1, 1:400, Abcam; mouse anti-CD68 ...

CMTr cap-adjacent 2’-O-ribose mRNA methyltransferases are required for reward learning and mRNA localization to synapses

Irmgard U. Haussmann, et al., bioRxiv - Molecular Biology 2021

Quote: ... Chromosome were blocked in PBT containing 0.2% BSA and 5% goat serum and sequentially incubated with primary antibodies (mouse anti-PolII H5 IgM, 1:1000, Abcam, and rat anti-HA MAb 3F10 ...

The small GTPase Arf1 regulates ATP synthesis and mitochondria homeostasis by modulating fatty acid metabolism

Ludovic Enkler, et al., bioRxiv - Cell Biology 2022

Quote: ... Sections were blocked with PBST (PBS+ 0.05% Tween 20) + 2% BSA for 15 min and incubated 3h at RT with anti-GFP antibody (1:100, 6556 Abcam) in PBST/BSA ...

Protective anti-prion antibodies in human immunoglobulin repertoires

Assunta Senatore, et al., bioRxiv - Neuroscience 2020

Quote: ... sections were blocked and incubated with the anti-PrP Fabs at 12 µg/ml and antibody for Microtubule Associated Protein 2 (MAP2) (1∶500, Abcam). Double staining was performed with fluorescently-labeled secondary antibodies (goat anti Human IgG F(ab’)2:Tritc 1:150 ...

VAL genes regulate vegetative phase change via miR156-dependent and independent mechanisms

Jim P. Fouracre, et al., bioRxiv - Plant Biology 2021

Quote: ... 2% was removed as input and samples were incubated overnight with 1% antibody (for histone ChIP: anti-H3 (abcam ab1791), anti-H3K27me3 (EMD Millipore 07-449) ...

Cardiac Microlesions Form During Severe Bacteremic Enterococcus faecalis Infection

Armand O. Brown, et al., bioRxiv - Microbiology 2020

Quote: ... paraffin embedded sections were treated with 1:100 rabbit Anti-mouse MLKL (phospho S345) antibody [EPR9515(2)] in order to detect phosphorylated MLKL (Abcam), and 1:100 rat anti-mouse MLKL antibody (Millipore-Sigma ...

Dysfunctions of the paraventricular hypothalamic nucleus induce hypersomnia in human and mice

Chang-Rui Chen, et al., bioRxiv - Neuroscience 2021

Quote: ... Primary antibodies were washed five times with PBS before incubation with secondary antibodies at room temperature for 2 h (Alexa 488, 1:1000; abcam). Finally ...

Glycomic analysis of host-response reveals high mannose as a key mediator of influenza severity

Daniel W. Heindel, et al., bioRxiv - Microbiology 2020

Quote: ... cells were stained with mouse monoclonal anti-MBL (Biotin) (2 μg/ml in MBL2 wash buffer; 1 h, 37 °C; Abcam) and mouse monoclonal anti-influenza A that had been labeled with Cy3 dye and dialyzed following the manufacturers protocol (1 μg/ml in MBL2 wash buffer ...

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