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Citations for abcam :
501 - 550 of 567 citations for L Asparagine H2O 13C4; D3; 15N2 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... Sections were incubated at room temperature for ~3 hours in secondary antibody solution containing goat anti-rabbit IgG H&L Alexa Fluor 488 (abcam; ab150077).
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bioRxiv - Evolutionary Biology 2022Quote: One hundred µl of the cell culture was transferred into a 96-well microplate provided by the In-Cell ELISA kit (Abcam, ab111541), and treated following the manufacturer’s recommendations ...
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bioRxiv - Neuroscience 2023Quote: ... the sections were incubated with secondary antibodies Goat Anti-Mouse IgG H&L (Alexa Fluor® 647) (1:700; Abcam, ab150115) and Goat Anti-Rabbit IgG Fc (Alexa Fluor® 488 ...
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bioRxiv - Cancer Biology 2023Quote: ... Myotubes were washed with PBS and incubated with anti-mouse IgG H&L Alexa Fluor 594 secondary antibody (1:2000) (Abcam, ab150116) and DAPI (1:3000 ...
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bioRxiv - Cancer Biology 2023Quote: ... Sections were washed with PBS and incubated in the blocking solution containing anti-goat IgG H&L HRP-conjugated secondary antibody (1:1000) (Abcam, ab6885) for 1hr ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were washed with PBS and incubated with anti-mouse IgG H&L Alexa Fluor 594 secondary antibody (1:2000) (Abcam, ab150116) and DAPI (1:3000 ...
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bioRxiv - Cancer Biology 2023Quote: ... the slides were incubated with the corresponding HRPconjugated secondary antibody for one hour at room temperature (goat anti-rabbit IgG H&L HRP, Abcam #ab6721). The slides were then washed in PBS 3 times then developed using the DAB substrate kit (Abcam #ab64238) ...
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bioRxiv - Cell Biology 2024Quote: The activity of Cathepsin L from isolated cardiomyocytes from 6-8 mice (plated as described) was assessed using the Cathepsin L Activity Assay kit from Abcam (ab65306). 50μg of protein from isolated cardiomyocytes were used to assess the degradation of the FR-AFC substrate at 37°C degrees for 2 hours ...
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bioRxiv - Cancer Biology 2024Quote: MKN7/HGC-27 cells that overexpressed or have knockdown of IGF2BP3 were collected and processed according to the kit instructions (Glucose Assay kit, Abcam, ab65333; L-Lactate Assay kit, Abcam, ab65331; ATP Assay Kit, Abcam, ab83355). In brief ...
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bioRxiv - Bioengineering 2024Quote: ... the slides were incubated with secondary antibody Goat Anti Rabbit IgG H&L (Alexa Fluor 488) (Abcam, ab150077, 1:500 dilution) for 30 min at 37℃ ...
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bioRxiv - Microbiology 2024Quote: ... The total concentrations of free amino acids and fatty acids per unit dry weight of activated sludge were measured by L-Amino Acid Assay Kit (Abcam, UK) and Free Fatty Acid Assay Kit (Abcam ...
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bioRxiv - Immunology 2024Quote: ... Detection of the rabbit pAb cocktail was conducted with a goat anti-rabbit IgG (H+L) AF647 secondary antibody (Abcam ab150079). Flow cytometry was used for quantifying binding using an Agilent NovoCyte Advanteon flow cytometer.
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bioRxiv - Cell Biology 2024Quote: ... and then washed 3X with PBST and incubated for 1 hour in secondary antibody Goat Anti-Mouse IGG H&L Alexa Fluor® 488 (ab150113, Abcam, 1 ...
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bioRxiv - Neuroscience 2024Quote: ... The secondary antibodies used were Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (1:500; Cat# ab150077, Abcam, UK) and Goat anti-Rabbit IgG (H+L) ...
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bioRxiv - Biophysics 2024Quote: ... at a ratio of 1:5000 in Roti®-Block in TBS-T and the goat-anti-rabbit IgG H&L (HRP) preadsorbed (#ab7090, abcam) at a ratio of 1:20,000 in TBS-T as a secondary antibody.
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bioRxiv - Cell Biology 2024Quote: Fixed oocytes were incubated for 1hr in 25μl droplets of blocking solution (see above) at 37°C containing primary antibodies: mouse anti-Bub1 [2F9] (1:200, Abcam ab54893), rabbit anti-Ki67 (1:100) ...
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bioRxiv - Bioengineering 2024Quote: ... The plates were incubated with 100 µl of goat anti-human IgG (H+L) cross-adsorbed secondary Ab (Abcam, Cambridge, UK) at 1/10,000 dilution in TBS-T for 30 min at room temperature ...
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bioRxiv - Developmental Biology 2024Quote: ... The samples were washed four times with staining buffer before applying the secondary antibody (1:800 donkey anti-rat IgG H&L Alexa Fluor-647, Abcam, ab150155) for 1 hour at room temperature ...
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bioRxiv - Immunology 2021Quote: ... confluent monolayers of 293T Lenti-X cells were stained with 2G12 or PGT145 bNAbs and Goat Anti-Human IgG H&L (10 nm Gold) secondary Ab (Abcam, Cambridge, UK) and then fixed in 2.5% glutaraldehyde in cacodylate buffer 0.1 M ...
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bioRxiv - Developmental Biology 2020Quote: ... brains were incubated with conjugated fluorescent secondary Alexa Fluor 555 donkey anti-mouse IgG (H+L) (Abcam; Material No. ab150106; 1:250) for 2 days at 4 °C.
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bioRxiv - Bioengineering 2022Quote: ... and incubated for 1h at room temperature with anti-mouse or anti-rabbit IgG H&L secondary antibodies conjugated with Alexa Fluor™ 488 or Alexa Fluor™ 568 (goat, 1:500; Abcam). Slides were mounted with VECTASHIELD antifade mounting medium containing DAPI (Vector Labs ...
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bioRxiv - Biophysics 2022Quote: ... The cells were washed with PBS three times before labeling with goat anti-rabbit IgG H&L Biotin (ab97049; abcam, Cambridge, UK) at 1:500 dilution for 30 min at room temperature ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... sections were washed 3x with PBS with 0.1% Tween-20 before incubation with the secondary polyclonal Donkey Anti-Goat IgG H&L antibody conjugated to Alexa Fluor 555 (ab150130; Abcam; 1:200) or Donkey Anti-Rabbit IgG H&L antibody conjugated to Alexa Fluor 555 (ab150066 ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... cells were washed 3x with 100 μL PBS with 0.1% Tween-20 before incubation with the secondary polyclonal Donkey Anti-Goat IgG H&L antibody conjugated to Alexa Fluor 555 (ab150130; Abcam; 1:200) or Donkey Anti-Rabbit IgG H&L antibody conjugated to Alexa Fluor 555 (ab150066 ...
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bioRxiv - Bioengineering 2021Quote: ... and 1% penicillin/streptomycin in PBS (Ca2+,Mg2+)) containing 30 µg/mL Goat anti-human IgG H&L (Dylight® 488, Abcam ab96911) at a 1:1 ratio ...
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bioRxiv - Microbiology 2020Quote: ... for 2 h at room temperature and a secondary antibody (1:500; Donkey anti-mouse IgG H&L, Alexa Fluor® 568, Abcam #ab175472) for 1 h at room temperature in the dark ...
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bioRxiv - Microbiology 2020Quote: ... 293T cells were fixed and processed for immunostaining against dsRNA (Scicons, 10010500) and visualized with goat anti-mouse IgG H&L (Cy5 ®) (Abcam, ab6563). Images were taken using Nikon Eclipse Ti-E Spinning Disk under 20X and 60X ...
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bioRxiv - Cell Biology 2021Quote: ... lungs were incubated with conjugated fluorescent secondary Alexa Fluor 555 donkey anti-mouse IgG (H+L) (Abcam; Material No. ab150106; 1:250) for two days at 4 °C.
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bioRxiv - Biochemistry 2023Quote: The following secondary antibodies have been used for Western blotting: Goat Anti-Mouse IgG H&L (HRP) preadsorbed (Abcam, #ab7068; 1:10000), Recombinant Protein G (HRP ...
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bioRxiv - Bioengineering 2022Quote: ... anti-occludin antibody (ab216327, and goat anti-rabbit IgG H&L (Alexa Fluor 488, ab150077) antibody were all obtained from Abcam (Waltham, MA). Anti-tumor necrosis factor-α (aTNF ...
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bioRxiv - Neuroscience 2024Quote: ... Triton X-100 in PBS and incubated with donkey-anti-mouse IgG (H+L) Alexa Fluor 488 nm (1:500, Abcam, UK, ab150109), donkey-anti-rabbit IgG (H+L ...
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bioRxiv - Immunology 2024Quote: ... samples were treated with unconjugated affinity purified F(ab) fragment IgG anti-mouse (H+L) for 1 hr at RT (0.1 mg/ml, Abcam, cat no. ab6668). Mouse epithelium was stained with Alexa 633-conjugated phalloidin (Invitrogen ...
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bioRxiv - Molecular Biology 2024Quote: ... Secondary antibody: Goat Anti-Rabbit IgG H&L(Alexa Fluor® 647)preadsorbed (ab150083) at a dilution of 1:2000 (Abcam, USA).
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bioRxiv - Molecular Biology 2024Quote: ... Secondary antibody: Goat Anti-Rabbit IgG H&L(Alexa Fluor® 647)preadsorbed (ab150083) at a dilution of 1:2000 (Abcam, USA); Ro 3306 ...
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bioRxiv - Molecular Biology 2023Quote: A pair of parotid salivary glands were harvested from each mouse (n=5/group) and directly placed in 500 uL of Lactate Assay Buffer from the L-Lactate Assay Kit (ab65331, Abcam, Cambridge, UK), 10 uL of protease inhibitor (PI ...
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bioRxiv - Neuroscience 2023Quote: ... and secondary antibody solutions were added for 1 hour at room temperature: donkey anti-rabbit IgG H&L (Alexa Fluor 647) at 1:200 (Abcam, Cambridge, Massachusetts); donkey anti-mouse IgG H&L (Alexa Fluor 488 ...
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bioRxiv - Immunology 2022Quote: ... Slides containing 4 sections per slide and 8 sections per sample were incubated with primary antibodies at 4°C overnight and then washed three times with PBS before incubating them with goat anti-mouse IgG (H&L) secondary antibody (Alexa Fluor® 488 in 1% BSA PBST, 1:300; Abcam #ab150113) for 1 h at room temperature in the dark ...
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bioRxiv - Cell Biology 2023Quote: ... cells were stained for 30 min with polyclonal fluorescein-isothiocyanate (FITC)-conjugated goat anti-mouse IgG H&L specific antibody (Abcam, United Kingdom) diluted 1:50 in RS-BSA 0.1% ...
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bioRxiv - Plant Biology 2024Quote: ... using a solution containing goat polyclonal secondary antibody to mouse or rabbit IgG – H&L conjugated to Alexa Fluor 488 (Abcam AB150113, AB150077), added in a 1:100 dilution ...
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bioRxiv - Pathology 2024Quote: ... Sections were then incubated with fluorochrome conjugated secondary antibody – goat anti-rabbit IgG H&L (Alexa Fluor® 488, ab150077, Abcam, UK) diluted in 1:500 ratio with PBS at room temperature for 30 min in the dark ...
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bioRxiv - Synthetic Biology 2024Quote: ... cells were washed three times with 1X PBS and then incubated with the secondary antibody (Goat Anti-Rabbit IgG H&L, Alexa Fluor® 647, Abcam, ab150079) at a 1:500 dilution in 2% BSA in 1X PBS for 1 hour at room temperature in the dark ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... (Epitope V, novoprotein, DA119), RSV Pre-F Antibody (3C12) (Epitope Φ, novoprotein, DA101) and Rabbit Anti-Human IgG H&L (FITC) (abcam, ab6755), and ELISA with Respiratory Syncytial Virus preF Antibody Pair (Φ & □ ...
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bioRxiv - Immunology 2024Quote: ... The membranes were washed and incubated with the respective HRP-coupled secondary antibodies for one hour at room temperature: goat anti-rabbit IgG H&L (#ab205718, 1:5000; abcam; RRID: AB_2819160), Goat Anti-Mouse IgG (H + L ...
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bioRxiv - Genomics 2024Quote: ... and 800 μl of antibody buffer containing 1 μl of primary antibodies (normal rabbit IgG, Santa Cruz Cat no sc-2027, H3K27ac (Abcam, ab4729), H4K16ac (Abcam ...
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bioRxiv - Biophysics 2024Quote: ... and Alexa Fluor 488-labeled Goat Anti-Rabbit IgG (H+L) (Beyotime, Shanghai, China) and HaCaT cells were immune-stained with anti-pan cytokeratin mouse recombinant multiclonal antibody (Abcam, Cambridge, UK) and Alexa Fluor 647-labeled Goat Anti-Mouse IgG (H+L ...
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bioRxiv - Zoology 2024Quote: ... Samples were incubated with the primary antibody in 1% BSA in PBS overnight and then incubated during 1 hour at room temperature with a secondary antibody goat anti-mouse IgG H&L Alexa Fluor 488 (1:500; Abcam, Cat# ab150113) diluted in 1% BSA and then rinsed in PBS ...
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bioRxiv - Biophysics 2021Quote: ... the cells were blocked for 1 h at room temperature using an unconjugated goat anti-mouse IgG H&L antibody (polyclonal, 1:1000, Abcam ab6708, Cambridge, UK) diluted in previously mentioned blocking solution ...
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bioRxiv - Cell Biology 2020Quote: ... Antibody was added to each tube (10μL anti-Histone H3, Cell Signaling Technology, cat #4620; 4μg Normal Rabbit IgG, Cell Signaling Technology, cat #2729; 4μg anti-SNF2H, Abcam, ab72499). Two SNF2H IP replicates were performed ...
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bioRxiv - Neuroscience 2020Quote: ... This was followed by a 48h incubation in goat antirabbit IgG H&L (Alexa Fluor 555) antibody (1:500, Abcam, Abcam Cat# ab150078, RRID:AB_2722519) and then placed for 1 h in a Hoescht:PBS (1:10 000 ...
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bioRxiv - Systems Biology 2023Quote: ... the cells underwent an additional 30-minute blocking step using 1× blocking buffer and were then incubated for 1 hour with secondary antibodies – Goat Anti-Mouse IgG H&L (Alexa Fluor® 594, Abcam, Cambridge, UK), and Phalloidin-iFluor 488 Reagent (Abcam ...