Labshake search
Citations for abcam :
5201 - 5250 of 10000+ citations for PAS Domain Containing Serine threonine Protein Kinase PASK Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2023Quote: ... Protein bands were separated by running the gel and Instant Blue Coomassie stain (ab119211, abcam) was used to visualise bands ...
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bioRxiv - Neuroscience 2023Quote: ... Protein bands were visualized using horseradish peroxidase-conjugated anti-mouse or anti-rabbit IgG (Abcam) and ECL (Thermo Fisher Scientific) ...
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bioRxiv - Biochemistry 2023Quote: ... 5 μL reactions were prepared with varying concentrations of recombinant human IRF5 protein (Abcam, ab173024) and recombinant human STK25 protein (Signalchem ...
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bioRxiv - Plant Biology 2023Quote: ... The membrane loaded with the proteins was incubated with either anti-GFP (1:5000, Abcam) or anti-tubulin (1:5000 ...
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bioRxiv - Neuroscience 2024Quote: ... Proteins transferred onto a nitrocellulose membrane were immunoblotted with anti-TBR2 (1:1000; ab183991; abcam), ROBO1 (1:2000 ...
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bioRxiv - Microbiology 2024Quote: ... 10mM EDTA pH 8) and protein quantification was determined with the bicinchoninic acid kit (Abcam). For each condition ...
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bioRxiv - Molecular Biology 2021Quote: ... Primary antibody incubations took place in a humid chamber at 4°C overnight with the following antibodies: chicken anti-GFP (Abcam ab13970 1:2000), mouse anti-mCherry (ab125096 1:1000) ...
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bioRxiv - Biophysics 2019Quote: ... followed by overnight incubation at 4 °C with the primary target antibody: rabbit anti-GFP polyclonal antibody (pAb) (1:1000; ab290, Abcam, Cambridge, United Kingdom), mouse anti-SERCA2 monoclonal antibody (mAb ...
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bioRxiv - Bioengineering 2022Quote: ... Mouse monoclonal anti-bassoon antibody was used as a presynaptic marker while rabbit polyclonal anti-homer1 antibody was used as a postsynaptic marker (Abcam, cat# ab82958, ab97593). For the immunostaining assay ...
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bioRxiv - Molecular Biology 2022Quote: ... Membranes were striped from anti N-VEGF or VEGF polyclonal antibodies and following re-blocking reacted with primary antibodies directed against Lactate Dehydrogenase (anti-LDH) (Abcam, cat #ab47010,1:300) and secondary antibodies anti-goat (1:10,000) ...
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bioRxiv - Neuroscience 2020Quote: ... bovine serum albumin (BSA) they incubated (4°C overnight) separately with specific primary antibodies including rabbit anti-tau polyclonal antibody (1:500, Abcam, Catalogue no. ab64193), rabbit anti GRP87/Bip polyclonal antibody (1:600 ...
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bioRxiv - Molecular Biology 2019Quote: ... Primary antibodies were incubated at 4°C overnight diluted as noted in Odyssey blocking buffer: rabbit monoclonal anti-Vinculin antibody (abcam, Cambridge, MA [EPR8185]) at 1:3000 ...
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bioRxiv - Cancer Biology 2020Quote: ... according to manufacturer’s instructions using human anti-PD-L1 antibody (Avelumab, EMD Serona, 1 μg/ml) or rabbit monoclonal Na/K ATPase antibody (Abcam # ab76020, 1:100,000) as primary antibodies and peroxidase-conjugated donkey anti-human IgG-HRP (Jackson ImmunoResearch ...
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bioRxiv - Pathology 2021Quote: ... blots were successively incubated with specific anti-ASIC2a antibody (Alomone labs, ASC-012, 1/1000) or pan-ASIC2 antibody (Abcam, ab77384, 1/700) and anti-rabbit IgG antibody coupled to horseradish peroxidase (Promega France ...
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bioRxiv - Neuroscience 2021Quote: ... Staining for tyrosine hydroxylase (primary antibody 1:500, chicken anti-TH, Aves Labs TYH; secondary antibody, 1:1000, goat anti-chicken, Alexa Fluor® 568, Abcam ab175477) was co-localized with YFP (primary antibody 1:500 ...
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bioRxiv - Cell Biology 2021Quote: ... Membranes were then probed with Alexa Fluor anti-rabbit A680 secondary antibody and Alexa Fluor anti-mouse A790 secondary antibody (Abcam ab175773 and ab175783). Membranes were visualized using an Odyssey Infrared Imaging System (LI-COR) ...
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bioRxiv - Microbiology 2020Quote: ... T cells were detected in sections from TgBAC(lck:EGFP)vcc4 zebrafish by anti-GFP staining to enhance visible fluorescent green signal (primary antibody: ab13970, Abcam; secondary antibody: ab150173, Abcam), stained slides were then mounted with coverslips using Fluoromount G containing DAPI ...
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bioRxiv - Cell Biology 2022Quote: ... milk and the membranes incubated at 4°C overnight with rabbit Anti-Annexin A1/ANXA1 antibody monoclonal antibody [EPR19342]-BSA and Azide free (1: 25000, Abcam, Cat. N. ab222398), ALIX (1:1000 ...
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bioRxiv - Cell Biology 2022Quote: ... For tissue sections we used KISS1 rabbit polyclonal antiserum antibody (Cat # AC566, 1:10,000) (Clarkson et al., 2009) and KISS1R rabbit polyclonal antibody (ABCAM, Cat # ab137483, 1:100). For cultured HESCs ...
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bioRxiv - Plant Biology 2022Quote: ... Shanghai, CN), Goat Anti-Mouse IgG HRP (Abmart, Shanghai, CN) and Anti-Myc antibody (HRP Anti-Myc tag antibody, Abcam, Cambridge, MA, USA).
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bioRxiv - Microbiology 2022Quote: ... LTA was detected with an anti-LTA primary antibody (mAb 55; HycultBiotech; 1:5000 dilution) and an HRP-goat anti-mouse IgG secondary antibody (Abcam; 1:10000 dilution). LTA was visualised using Amersham ECL Prime western blotting detection reagent (GE Healthcare ...
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bioRxiv - Neuroscience 2022Quote: ... followed by overnight incubation with primary antibodies (CDKL5- 1:1000, #HPA002847, Atlas Antibodies-Sigma Aldrich; NMDAR1-1:1000, #700685, Thermo Fisher; NMDAR2A-1: 1000, #ab169873, Abcam; NMDAR2B- 1:1000 ...
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bioRxiv - Molecular Biology 2024Quote: ... standard CUT&RUN102 was performed using the following antibodies (1 µg per CUT&RUN sample if not stated otherwise): rabbit monoclonal anti-TAF1 antibody (in-house/Abcam, cTAF1 177-493), rabbit polyclonal anti-TAF2 antibody (pAb 302853) ...
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bioRxiv - Biophysics 2023Quote: ... Blots were probed with antibodies for XRCC4 (CBMAB-X0197-YC, 1:2000), XLF (Antibodies ABIN2857060, 1:2000) and MLH1 (Abcam ab92312, 1:1000). Agilent NovoExpress Version 1.5.0 for the Quanteon was used to capture and analyze the flow cytometry data ...
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bioRxiv - Cell Biology 2023Quote: ... The membranes were blocked in 5% skim milk solution for 1 h at RT then incubated with primary antibody at 4 ℃ overnight: Anti-FMRP antibody (Abcam, ab17722, 1:1,000), anti-GAPDH antibody (Cell signaling technology ...
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bioRxiv - Molecular Biology 2022Quote: ... Single and double immunohistochemical reactions were performed as described previously (Rueda-Alanã and Garciá-Moreno, 2021) using the following primary antibodies: rabbit antibody to histone H3 - phospho S10 (Abcam ab47297; 1:1000), mouse antibody to LHX5 (DSHB;1:30 ...
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bioRxiv - Biochemistry 2023Quote: ... The cell lysates were boiled in SDS-PAGE loading buffer for 10 min and then subjected to SDS-PAGE and probed with the following specific antibodies: mouse anti-PrP monoclonal antibody 8H4 (Abcam ab61409, 1:5,000), mouse anti-MyHC antibody MF-20 (Developmental Studies Hybridoma Bank MAB4470-SP ...
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bioRxiv - Cell Biology 2024Quote: Anti-ATG7 antibody (Cat. NO. ab133528; clone name, EPR6251) and anti-SQSTM1/p62 antibody (Cat. NO. ab91526) were from Abcam (Waltham, MA, USA). LC3A/B XP Rabbit mAb (Cat ...
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bioRxiv - Bioengineering 2023Quote: ... After completion of blocking the membrane was incubated in primary antibody overnight on a shaker (Primary antibodies: Syntenin-1 (Abcam, ab133267 1:2000); CD81 (Santa Cruz ...
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bioRxiv - Cell Biology 2024Quote: ... Goat anti-rabbit IgG (Alexa Fluor 488) antibody (ab150081) and goat anti-mouse IgG (Alexa Fluor 594) antibody (ab150120) were from Abcam (Cambridge, MA, USA). SAG (Smoothened Agonist ...
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bioRxiv - Biophysics 2024Quote: ... The primary antibodies were subsequently recognized by a goat anti-human IgG secondary antibody conjugated with DyLight® 650 fluorescent label (Abcam, Cambridge, UK). Cell nuclei were stained with DAPI (Beyotime ...
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bioRxiv - Cancer Biology 2024Quote: ... primary antibodies were at incubated at 37°C for 60 minutes under the following conditions: anti-S100P antibody (Abcam, ab124743, 1:2000 dilution), anti-SERPINB5 antibody (Abcam ...
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bioRxiv - Neuroscience 2024Quote: ... It was released by confirming the identity of HC-HA/PTX3 based on Western blot analysis using respective antibody specific to HC1 (ITIH1 antibody, Cat# ab70048, Abcam, Waltham, MA, USA) and PTX3 (PTX3 antibody ...
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bioRxiv - Molecular Biology 2024Quote: ... Whole cell lysates of SH-SY5Y cells (10 µg) were subjected to Western blotting using the following antibodies: mouse anti-SLP-2 antibody (Abcam ab89025, 1:1000), rabbit anti-HSP60 (Cell Signaling #4870 ...
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bioRxiv - Developmental Biology 2020Quote: ... Control and repo mutant embryos containing plasmatocytes labelled via crq-GAL4,UAS-GFP were fixed and immunostained using mouse anti-GFP (ab1218 1:200; Abcam) and rabbit anti-cleaved DCP-1 (9578S 1:1000 ...
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bioRxiv - Molecular Biology 2021Quote: ... the 5’ adapter (containing four random nucleotides at the 3’ end) was ligated to the small RNAs using T4 RNA ligase (Abcam) for 1h ...
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bioRxiv - Biochemistry 2020Quote: ... The cells were differentiated in a medium containing 1% Fetal Calf Serum supplemented with 10 μM trans-Retinoic acid (Abcam) for 5 days ...
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bioRxiv - Biochemistry 2020Quote: ... Coverslips were then incubated for 30 min with 50 μl solution containing 1% BSA in PBT and streptavidin-Alexa 488 conjugate (Abcam) diluted 1:150 ...
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bioRxiv - Biochemistry 2020Quote: ... The cells were differentiated in a medium containing 1% Fetal Calf Serum supplemented with 10 μM trans-retinoic acid (Abcam) for 5 days ...
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bioRxiv - Neuroscience 2020Quote: ... The sections were washed with PBS for 3 times (10 min each) and incubated at room temperature for 1 hour in blocking solution containing 10% normal horse serum (Abcam), 0.2% bovine serum albumin (Sigma) ...
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bioRxiv - Neuroscience 2020Quote: ... currents elicited by 10 ms-pulse stimulation of ChR2-containing axonal terminals and isolated by blocking AMPA/KARs (10 µM NBQX, Abcam), NMDARs (50 µM D-APV ...
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bioRxiv - Cancer Biology 2020Quote: ... cells were incubated for 60 min with blocking solution (PBS containing 1% (w/v) BSA and 10% (v/v) normal goat serum (ab7481, Abcam)) ...
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bioRxiv - Biophysics 2020Quote: ... which was followed by the media replacement with a fresh media containing FCS reduced to 1% and supplemented with 10 μM trans-Retinoic acid (Abcam) for 5 days ...
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bioRxiv - Cell Biology 2020Quote: ... cell culture medium was aspirated and iPSCs were incubated in 2 mM EGTA (Carl Roth) solution in PBS for 20 mins followed by incubation in culture medium containing Y-27632 ROCK inhibitor (Abcam). Control samples received knockout DMEM (Gibco ...
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bioRxiv - Immunology 2021Quote: ... Cells were washed twice for 10 min with PBS and a cover slip was mounted to a glass slide with a mounting solution containing DAPI (Abcam). Cells were visualized using confocal microscopy and VE cadherin surface expression quantified using ImageJ 1x (Image J ...
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bioRxiv - Systems Biology 2021Quote: ... All primary and secondary antibodies were diluted 1:400 in PBS containing either 2.25% bovine serum albumin [Millipore Sigma #A2058] or 5% normal goat serum (Abcam #ab7481) for blocking and 0.15% Triton X-100 (Sigma Aldritch #T8787 ...
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bioRxiv - Microbiology 2020Quote: ... which are the ratio of sample serum absorbance to the absorbance of a manufacturer-provided solution containing the upper limit of the reference range of non-infected biological sample (“cutoff” (Abcam) or “calibrator” (Euroimmun)) ...
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bioRxiv - Neuroscience 2022Quote: Immunohistochemistry was performed for neuroblast detection and analysis of hippocampal neurogenesis using the following primary antibodies diluted in PBS containing 5% goat serum and 0.1% Triton-X-100: anti-doublecortin (DCX, rabbit: 1:5000, Abcam, #ab18723), anti-bromodeoxyuridine (BrdU ...
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bioRxiv - Biochemistry 2022Quote: SISA strains KS8266 and KS8311 (Mustavchiev et al., 2016) were maintained on Ye5S media containing 5μM 3-BrB-PP1(Abcam, ab143756) to prevent activation of sty1 and retention of Wis1DD was confirmed by sequencing of cells used for experiments ...
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bioRxiv - Physiology 2022Quote: ... slides were washed 3 × 1 minute in PBS before exposing to a blocking solution containing TBST (TBS with 0.1% Tween-20) plus 20% AquaBlock (Cat. # ab166952, Abcam USA) for 30 minutes ...