Labshake search
Citations for BioSpec :
51 - 100 of 222 citations for 6 4 METHOXY PHENYL 2 PHENYL PYRAZOLO 1 5 A PYRIMIDINE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2023Quote: ... and added to a 2-ml screw-cap tube containing zirconia beads (BioSpec Products). Cells were disrupted by bead-beating three times for 30 seconds ...
-
bioRxiv - Microbiology 2023Quote: ... Approximately 2 × 108 conidia were homogenized using a Mini-Bead beater (BioSpec Products Inc.) with 0.3 mL of glass beads (Daihan Scientific ...
-
bioRxiv - Plant Biology 2023Quote: ... Baker) in 2 L flasks with the addition of 0.5 mm glass beads (BioSpec) and shaken 20 °C overnight ...
-
bioRxiv - Molecular Biology 2023Quote: Approximately 5 mg of frozen heart LV tissue was homogenized using a BeadBeater (BioSpec, Bartlesville, OK, USA) with zirconia/silica beads in 1 mL RLT buffer (Qiagen ...
-
bioRxiv - Molecular Biology 2021Quote: ... Cells were pelleted at 6000 rpm for 2 minutes at 4°C and the pellet was resuspended in FA lysis buffer and Zirconia beads (0.7 mm diameter, Biospec). Cells were broken using a cell homogenizer (Bertin Instruments ...
-
bioRxiv - Microbiology 2019Quote: ... Tissues were collected in screwcap tubes containing 500 μl SPGS and 3-4 2.0 mm zirconia beads (BioSpec Products) and homogenized using a Bead Mill 24 (Fisher Scientific ...
-
bioRxiv - Genomics 2022Quote: ... Cells were lysed at 4°C by repeated vigorous agitation with 0.5mm zirconia/silica beads in a bead beater (Biospec) using 4 cycles of 60 s with 75 s of rest in between cycles ...
-
bioRxiv - Genomics 2022Quote: ... Final suspensions were transferred to 2 ml tubes containing 0.5 mm zirconia/silica beads (BioSpec, Lab Services BV ...
-
bioRxiv - Microbiology 2023Quote: ... Pellets were then bead beaten (2 x one minute) using 0.1mm zirconia/silica beads (BioSpec). Triton-X-100 was then added to lysates at a final concentration of 1% to solubilize membrane proteins and vortexed to mix.
-
bioRxiv - Microbiology 2024Quote: ... RNAse-free 2 mL tube containing around 100 μg of 0.1 mm silica beads (Biospec) and 3.5 μL of β-mercaptoethanol (ROTH ...
-
bioRxiv - Bioengineering 2022Quote: ... 5 mM EDTA) containing 140 μg/ml papain using steel balls and a Mini-BeadBeater™ (Biospec, USA). Samples were incubated at 60°C overnight in a water bath shaker (300 RPM) ...
-
bioRxiv - Plant Biology 2022Quote: ... Seedlings were flash-frozen in liquid nitrogen for 5 min and crushed using a Mini-BeadBeater (Biospec Products) for 1 min ...
-
bioRxiv - Evolutionary Biology 2024Quote: ... This was carried out by bead beating for 5 minutes using a mixture of 0.1 and 0.5mm zirconia/silica beads (BioSpec) in a BioSpec Mini-Beadbeater ...
-
bioRxiv - Microbiology 2024Quote: ... and 500 µL phenol:chloroform:isoamyl alcohol (pH 4.5) and bead beat on high for 5 minutes (Minibeadbeater, Biospec Products). Samples were centrifuged at 18,000xg for 3 minutes at 4°C ...
-
bioRxiv - Microbiology 2021Quote: ... The plate was shaken violently for 4 min at 2100 rpm on a bead beater (Biospec Mini-beadbeater-96, #1001) to homogenize the flies ...
-
bioRxiv - Genetics 2020Quote: ... were resuspended in 1 ml of 155 mM ammonium formate and lysed at 4 °C with 400 µl of acid-washed glass beads using a Mini-Beadbeater (Biospec). Lysates corresponding with 0.4 OD units were subsequently spiked with internal lipid standards and subjected to two-step lipid extraction [68] ...
-
bioRxiv - Genetics 2020Quote: ... flies were homogenized with 3-4 glass beads in 600μL distilled water for 45 seconds using a Mini-Beadbeater-96 (BioSpec Products). Homogenate was centrifuged for 5 minutes at 14,000rpm ...
-
bioRxiv - Bioengineering 2022Quote: ... Each hMSC seeded scaffold was placed in a 2 mL reinforced microvial (BioSpec Products, Oklahoma, USA) containing four 3.2 mm stainless steel beads and 1 mL of TRIzol™ Reagent (ThermoFisher Scientific ...
-
bioRxiv - Immunology 2023Quote: ... using the Mobio PowerSoil DNA extraction kit (MoBio) following a 2-minute bead-beating step (Biospec). The V4 of the 16S rRNA gene was amplified using PCR with barcoded primers ...
-
bioRxiv - Microbiology 2024Quote: ... The Mtb samples (200 µl) were placed in 2 ml Polypropylene Microvials (Cat#10832, BioSpec Products) containing approximately 70 µl of 0.1 mm Zirconia/Silica beads (Cat# 11079101z ...
-
bioRxiv - Microbiology 2019Quote: ... 700 μL of cold methanol and 140 μL of EDTA 0.1M were added and vigorously mixed (4 x 45 s) in a mini-bead-beater-8 (Biospec Products, Qiagen). By this way ...
-
bioRxiv - Bioengineering 2021Quote: ... and a relationship of 0.9 mL of S30B and 5 g of 0.1 mm diameter silica beads (Biospec, 11079101) were added per gram of pellet obtained ...
-
bioRxiv - Neuroscience 2023Quote: ... according to the manufacturer’s instructions and following a 2-minute bead beating step (BioSpec, Bartlesville, OK, USA). Purified DNA was used for PCR amplification of the variable V4 region using the 515F and 806R barcoded primers following the Earth Microbiome Project protocol (Human Microbiome Project Consortium ...
-
bioRxiv - Genetics 2020Quote: ... Approximately 30-100 mg of material was homogenized in QIAGEN’s DNEasy Plant Mini Kit extraction buffer using a BeadBeater with 4 × 3.2 mm steel beads in XXTuff 2mL O-ring screw cap tubes (Biospec, Bartlesville, OK, USA). Following homogenization ...
-
bioRxiv - Bioengineering 2022Quote: ... were washed in PBS and disintegrated in 0.5 mL of 0.2% (v/v) Triton X-100 and 5 mM MgCl2 solution using steel beads and a Mini Beadbeater™ (Biospec). The remaining solution was centrifuged at 3000g for 10 minutes ...
-
bioRxiv - Microbiology 2023Quote: ... The lungs and spleens were harvested in 5 mL lysis buffer (0.1% IGEPAL, in 1x PBS sterile filtered) and homogenized using a Tissue Teaeror Homogenizer (BioSpec Products). Organ homogenate was serially diluted into 1X PBS and dilutions plated on TSAC ...
-
bioRxiv - Microbiology 2023Quote: Mouse fecal samples (30-60 mg of preweighed material) were homogenized with bead beating for 5 min (Mini-Beadbeater-96, BioSpec) using the ZR BashingBead lysis matrix containing 0.1 and 0.5 mm beads (ZR-96 BashingBead Lysis Rack ...
-
bioRxiv - Microbiology 2021Quote: Samples were bead beaten on high for 2-3 minutes with a Mini-BeadBeater-16 (Biospec Products, USA), then centrifuged at 18,000 g for 5 mins ...
-
bioRxiv - Microbiology 2020Quote: ... samples were bead beaten on the homogenize setting for 2 minutes using a mini-bead beater (BioSpec Products). For a subset of isolates DNA was extracted with either the Qiagen Plant DNeasy (QIAGEN ...
-
bioRxiv - Molecular Biology 2020Quote: ... transferred to 2-ml screw-cap tubes and disrupted using zirconium/silica beads 0.5 mm (BioSpec Products, #11079105z) by bead beating 7 times 3 minutes in a Genie Disruptor (Scientific Industries) ...
-
bioRxiv - Molecular Biology 2021Quote: ... Cell suspension was transferred to two 2 ml skirted screw-cap tubes containing 1.25 ml zirconia beads (0.5 mm, BioSpec) and additional buffer added to completely fill tube ...
-
bioRxiv - Microbiology 2023Quote: ... 200 μl saliva sample was diluted in 1000μl tris-EDTA (TE) buffer (pH 8.0) in a 2 ml sterile screw cap vial (Biospec) and centrifuged at 10,000g for 5 min ...
-
bioRxiv - Bioengineering 2021Quote: ... were washed with PBS and each disintegrated in 500 μL of 0.2% (v/v) Triton X-100 and 5 mM MgCl2 solution using steel balls and a minibeadbeater™ (Biospec, USA). The solids were separated by centrifugation (3000 g ...
-
bioRxiv - Microbiology 2019Quote: ... This included a combination of 5 min of heat (65°C) followed by 5 min of bead-beating for cell disruption on a Mini-Beadbeater-24 (Biospec, U.S.A.). After extraction the DNA was checked for quality by gel electrophoresis and quantified using a Qubit 4 (Thermo Fisher Scientific ...
-
bioRxiv - Systems Biology 2022Quote: ... using pre-assembled tubes containing 0.5g zirconia/silica beads (0.1 mm) and 5 glass beads (2.7 mm) (Biospec products, Bartlesville, OK, USA). In tubes without stabilization buffer ...
-
bioRxiv - Genomics 2023Quote: ... 1 ml of 0.5 mm zirconia/silica beads was added to the tube and cells were lysed in a Mini-BeadBeater 24 (BioSpec products) for three cycles (3 min ON/5 min OFF) ...
-
bioRxiv - Molecular Biology 2023Quote: ... at 6.0 m/s (3 x 30 s, 5 min pause time) using 1.0 mm zirconia/glass beads (Biospec Products, OK, USA). After centrifugation for 10 min at 15,000 x g and 4 °C with a 5415R benchtop microcentrifuge (Eppendorf ...
-
bioRxiv - Cell Biology 2023Quote: ... the cell pellets were resuspended in 500 μl of ice-cold 5% [w/v] 5-sulfo-salicylic acid dehydrate (SSA) and homogenized by vortexing in the presence of 300 µL zirconium beads (BioSpec Products) at 4°C for 30 min ...
-
bioRxiv - Neuroscience 2021Quote: ... brain hemispheres were transferred to a 2 mL reinforced polypropylene tube (Cat. No. 330TX; BioSpec Products, Inc., Bartlesville, OK) pre-filled with 1 mL of homogenization buffer (101 mM NaCl ...
-
bioRxiv - Molecular Biology 2019Quote: ... 2% Triton X-100) and cells were lysed in the presence of 0.3mm glass beads (Biospec products, Inc. 11079105). Phenol-chloroform (50:50 ...
-
bioRxiv - Pharmacology and Toxicology 2020Quote: ... added into a 2 mL tube with 3.5 g of 2.3 mm zirconia/silica beads (BioSpec Producs, Bartlesville, OK), and homogenized at 4800 rpm for 20 seconds ...
-
bioRxiv - Microbiology 2022Quote: ... This suspension was transferred into a sterile 2 mL bead beating tube containing 0.5mm Zirconia-Silicate beads (Biospec, USA). The bead beating was carried on in a Mini-beadbeater (BioSpec ...
-
bioRxiv - Microbiology 2023Quote: Cell pellets from 10 ml cultures were resuspended in 0.7 ml of MDC and transferred to 2-ml screw-cap microcentrifuge tubes containing ∼0.25 ml of 0.1 mm Zirconia/Silica beads (BioSpec Products). Cells were lysed in a 4°C room by 8-rounds of bead-beating using a FastPrep®-24 homogenizer (MP Biomedical ...
-
bioRxiv - Animal Behavior and Cognition 2024Quote: ... 1 mm zirconia (BioSpec Products) and approx ...
-
bioRxiv - Developmental Biology 2019Quote: ... dissected digestive tracts were dissected and pooled (5 guts/pool) into 1mL sterile phosphate buffered saline (PBS) which was then mechanically disassociated using a Tissue-Tearor (BioSpec Products, 985370). 100 µL of serially diluted solution was then spotted on a Tryptic soy agar (TSA ...
-
bioRxiv - Bioengineering 2021Quote: ... were washed with PBS and each disintegrated in 500 μL of 5% Trichloroacetic acid (TCA) in UPW using steel balls and a minibeadbeater™ (Biospec, USA). After 48 hours of incubation at room temperature ...
-
bioRxiv - Microbiology 2020Quote: ... each containing 5 infected eyes in 400 μl of sterile PBS and 1.0 mm sterile glass beads (Biospec Products Inc., Bartlesville, OK), were homogenized for 60 seconds at 5,000 rpm in a Mini-BeadBeater (Biospec Products ...
-
bioRxiv - Bioengineering 2022Quote: ... and each was disintegrated in 500 μl of 5% trichloroacetic acid (TCA) in UPW using steel balls and a Mini-BeadBeater™ (Biospec, USA). After 48 hours of incubation at room temperature ...
-
bioRxiv - Developmental Biology 2023Quote: ... dissected digestive tracts were dissected and pooled (5 guts/pool) into 1mL sterile phosphate buffered saline (PBS) which was then mechanically disassociated using a Tissue-Tearor (BioSpec Products, 985370). 100 µL of serially diluted solution was then spotted on a Tryptic soy agar (TSA ...
-
bioRxiv - Cancer Biology 2021Quote: ... was performed 21 days after tumor implantation in mice under Isofluorane anesthesia (2 to 2,5% mixed in ambient air) in a 4.7-T magnetic resonance scanner (Bruker BioSpec 47/40USR). Brain images were obtained using a Fast-Spin-Echo (FSE ...