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Citations for BioSpec :
501 - 542 of 542 citations for Hexadecanoic acid reaction products with tetraethylenepentamine since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... We ground the plant tissues in a 2.0 ml screw tube with one 3.2 mm diameter stainless steel bead and two 2.3 mm diameter zirconia/silica beads (BioSpec Products, Bartlesville, Oklahoma, USA). The tissues were ground at 60 Hz for 30 seconds three times using a STEP tissue grinder (ACTTR ...
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bioRxiv - Genomics 2024Quote: ... Mosquito samples were mechanically homogenised for two 3-minute cycles at 1,500 rpm using a Mini Beadbeater-96 (BioSpec Products, Bartlesville, USA) and centrifuged for two 5-minute cycles at 14,000 rpm ...
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bioRxiv - Microbiology 2024Quote: ... cecal contents were weighed and transferred to tubes containing 1.2 g of zirconia-silicate beads (Cat. No. 11079101z, BioSpec Products, Bartlesville, OK). Cecal contents were homogenized using a bullet blender and a vortex in 1 mL of 0.5% phosphoric acid per 100 mg of sample ...
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bioRxiv - Developmental Biology 2023Quote: ... dissected digestive tracts were dissected and pooled (5 guts/pool) into 1mL sterile phosphate buffered saline (PBS) which was then mechanically disassociated using a Tissue-Tearor (BioSpec Products, 985370). 100 µL of serially diluted solution was then spotted on a Tryptic soy agar (TSA ...
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bioRxiv - Immunology 2022Quote: ... lung cells in the single cell suspension were further homogenized with glass beads using Mini Bead Beater (BioSpec Products Inc, Bartlesville, OK) and resuspended in PBS ...
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bioRxiv - Microbiology 2024Quote: ... The samples were then bead-beaten for 2 minutes at 2400 rpm in a Mini-Bead-Beater 96 (Biospec Products, Bartlesville, USA) and subsequently incubated for 20 minutes at 56 °C ...
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bioRxiv - Microbiology 2024Quote: ... Briefly specimens were placed in RLT buffer and homogenized with a micro pestle followed by further homogenization using a Qiagen TissueLyser for 3 minutes at 30 Hz with 100 μL of 0.1 mm Mini-BeadBeater Zirconia-Silicate beads (BioSpec Products, Oklahoma, USA). The remaining steps were done following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2021Quote: ... mixed with 1 mL of pre-cooled methanol containing 0.5 μM of stable-isotope-labeled bile acids (internal standards) and 1.0 mm diameter zirconia/silica beads (BioSpec, Bartlesville, OK), followed by thorough homogenization and centrifugation ...
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bioRxiv - Genetics 2020Quote: ... were resuspended in 1 ml of 155 mM ammonium formate and lysed at 4 °C with 400 µl of acid-washed glass beads using a Mini-Beadbeater (Biospec). Lysates corresponding with 0.4 OD units were subsequently spiked with internal lipid standards and subjected to two-step lipid extraction [68] ...
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bioRxiv - Genetics 2023Quote: ... Cells were lysed using a FastPrep (MP) bead beater (5 x 90 second runs at 4°C) and acid-washed glass beads (BioSpec), centrifuged ...
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bioRxiv - Molecular Biology 2024Quote: ... The supernatant was transferred to pre-fill screw-capped vials with ∼250 μl volume of acid-washed beads (500 µm diameter, BIOSPEC). Cells walls were disrupted with Precellys evolution homogenizer (Bertin Technologies ...
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bioRxiv - Microbiology 2022Quote: ... placed in a microvial rack, and violently shaken (3 times 10 sec, 10 sec interval) in a bead beater (BioSpec Products; Mini Beadbeater) to disrupt the cells and tissues ...
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bioRxiv - Microbiology 2020Quote: Foliage and synthetic diet samples were placed in thick walled 2 ml tubes with three 2.3mm diameter stainless steel beads (BioSpec Products, Bartlesville, OK, USA) and 250 μl of the PowerSoil bead tube buffer ...
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bioRxiv - Plant Biology 2020Quote: ... Leaf discs were then ground in sterile water for 5 min using the tissue-lyser and metal beads (Biospec Products, Bartelsville, OK, USA). Serial dilutions of the homogenate were plated onto LB agar supplemented with either gentamicin (10 µg/ml ...
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bioRxiv - Microbiology 2020Quote: ... Experimental and control eyes were harvested and transferred to individual 1.5ml screw-cap tubes containing sterile 1mm glass beads (BioSpec Products, Inc., Bartlesville OK) and 400μl lysis buffer (RLT ...
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bioRxiv - Microbiology 2021Quote: ... and placed individually in 1 ml of sterile DPBS containing zirconia/silica beads of diameter 2.3 mm (BioSpec Products, Inc, Bartlesville, OK, USA). Organs were homogenized by bead-beating for one min and then placed on ice ...
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bioRxiv - Physiology 2021Quote: ... Adipocytes isolated from 200 mg of tissue in the previous step were homogenized in 2 ml of Trizol Reagent® using tissue-tearor homogenizer (Model 985370, BioSpec Products, Inc). After homogenization ...
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bioRxiv - Biophysics 2020Quote: ... Cells were broken by four cycles of 5sec ON and 5min OFF in an ice-cooled glass bead homogenizer (Bead-Beater, BioSpec Products, Bartlesville, OK). After breakage the sample was centrifuged at 3,600g for 10 min to pellet unbroken cells and cell debris ...
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bioRxiv - Microbiology 2020Quote: ... Whole-cell lysates were prepared from the cross-linked and untreated cell suspensions by glass bead breakage in a Mini-Bead Beater 8 apparatus (BioSpec Products, Inc., Bartlesville) for four 40 second cycles with 1 min cooling on ice between each cycle ...
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bioRxiv - Microbiology 2020Quote: ... and 5 eyes were placed into each of 5 separate tubes containing 400 μl of sterile PBS and 1.0 mm sterile glass beads (Biospec Products Inc., Bartlesville, OK), and homogenized for 60 seconds at 5,000 rpm in a Mini-BeadBeater (Biospec Products ...
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bioRxiv - Microbiology 2021Quote: ... bladders and kidneys were removed from each mouse and homogenized in tubes containing 1.0mm diameter zirconia/silica beads (Biospec Products, catalog no. 11079110z) using a MagNA Lyser (Roche Diagnostics) ...
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bioRxiv - Biochemistry 2020Quote: ... Cells were lysed in a 1.5 mL centrifuge tube with denaturing lysis buffer (8 M urea, 50 mM NH4HCO3, 10 mM IAA) and 0.5 mm glass beads (Biospec Products Inc., Bartlesville, OK). Protein concentration of yeast lysate was measured by a Coomassie stained SDS gel(Xu et al. ...
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bioRxiv - Microbiology 2023Quote: ... 20OD spore sample for each replicate was homogenized in 3mL ice-cold 60% ethanol aqueous solution with Zirconium-silica beads (0.1 mm, BioSpec Products, Bartlesville, OK, USA) in a screw-top tube ...
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bioRxiv - Bioengineering 2022Quote: ... 80 μL 1 M HCl, and 800 μL chloroform: methanol (ratio 80:20, v/v) and vigorously agitating in the Mini-Beadbeater 16 (BioSpec Products, Bartlesville, USA) for 4 min ...
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bioRxiv - Neuroscience 2022Quote: Frozen left cerebral hemispheres of mice were weighed and homogenized with 0.7 mm diameter zirconia beads (catalog number 11079107, BioSpec Products, Bartlesville, OK, USA) in a buffer composed of 100 mM Tris-HCl ...
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bioRxiv - Physiology 2024Quote: ... were collected into sterile microcentrifuge tubes and weighed before being homogenized in sterile endotoxin-free saline using sterile 2.3 mm zirconia/silica beads (Biospec Products Inc.; Bartlesville, OK), as previously described by Speer ...
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bioRxiv - Immunology 2024Quote: ... and ileal lymph nodes were removed and homogenized in tubes containing sterile PBS and 1.0-mm-diameter zirconia/silica beads (Biospec Products; catalog number 11079110z) using a MagNA Lyser instrument (Roche Diagnostics) ...
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bioRxiv - Microbiology 2020Quote: ... Samples were homogenised for 90 seconds with the addition of silica glass beads (Daintree Scientific, St Helens, TAS, Australia) in a MiniBeadbeater-96 (Biospec Products, Bartlesville, Oklahoma, USA). Total RNA was extracted from the homogenate according to the Trizol protocol ...
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bioRxiv - Microbiology 2020Quote: ... each sample was thawed on ice and homogenized for 30 seconds at maximum speed using a Mini-BeadBeater-8 (BioSpec Products Inc., Bartlesville, OK). DNA was extracted according to manufacturer specifications using the Zymo Quick-DNA Fungal/Bacterial Miniprep kit (Cat# D6005 ...
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bioRxiv - Evolutionary Biology 2022Quote: ... with one 2.5mm glass beads and 100µl cell lysis solution before homogenizing in a bead beater (Mini Beadbeater™, Biospec Products INC., Bartlesville, OK, USA). The rest of the protocol followed the standard Puregene Gentra Tissue kit manual.
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... whole lung tissue was placed into BioStor™ Vials with screw caps (National Scientific Supply, Claremont, CA, USA) with 3.5 g of zirconia/silica beads (BioSpec Products, Bartlesville, OK, USA). The tissue was homogenized at 4,800 rpm for 20 seconds.
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bioRxiv - Genomics 2021Quote: Genomic DNA was isolated using the CTAB protocol (Andreou, 2013) with minor modifications that included the use of 0.5 mm silica beads (Biospec Products, Inc. Cat. No. 110791052z), shaken using a specialized MO BIO Vortex-GenieR 2 (MO BIO Laboratories) ...
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bioRxiv - Microbiology 2022Quote: Virus nucleic acid was extracted using the Roche High Pure virus nucleic acid extraction kit by adding 200 µl of the working binding buffer to the tubes containing bodies or legs & wings and homogenizing the tissues by shaking the tubes for 1 min 30 s using a Mini Beadbeater-96 (BioSpec Products, Bartlesville, OK, USA). The tubes were centrifuged for 8,000 × g for 1 min ...
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bioRxiv - Bioengineering 2023Quote: DNA was extracted using a cetyl trimethylammonium bromide (CTAB) method51 with an additional bead-beating step (Mini-Beadbeater-96, BioSpec Products, Bartlesville, OK, USA) for 1.5 min using 0.1 mm diameter zirconium beads ...
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bioRxiv - Plant Biology 2024Quote: ... 10 mM EDTA) and transferred to 96-well plates containing 500 µL of 2 mm garnet rocks per well (Biospec Products, Bartlesville, OK, USA), after which DNA purification proceeded via bead-beating and chemical lysis as previously described (Wagner et al ...
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bioRxiv - Genomics 2024Quote: ... The pre-lysate was transferred into a 2mL screw cap tube containing 550mg of 0.5mm zirconia/silica beads (BioSpec Products, Inc.; Cat. No. 11079105Z). Bead-beating on the FastPrep-24 (MP Biomedicals ...
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bioRxiv - Genomics 2024Quote: ... Samples were transferred to 2mL screw cap tubes containing 550mg of autoclaved and air-dried 0.5mm zirconia/silica beads (BioSpec Products Inc.; Cat. No. 11079105Z). Bead-beating was carried out with 3 cycles of 1 min at 6m/s with 5 min interval on ice ...
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bioRxiv - Evolutionary Biology 2024Quote: We generated whole cell lysate by homogenizing with a bead beater at 3000 strokes per minute for 3 minutes with 1 mm glass beads (BioSpec Products catalog number 11079110) or until most cells appeared lysed under a microscope ...
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bioRxiv - Microbiology 2021Quote: ... 50 μL of 500 μm RNAse-free glass beads was added and the cell suspension was ground in 3 cycles of 30 sec using a BioSpec Mini-Beadbeater-16 (Biospec Products Inc., Bartlesville, OK, USA). The cell lysate was centrifuged to remove cell debris and the crude RNA extract collected from the supernatant ...
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bioRxiv - Bioengineering 2021Quote: ... were washed with PBS and each disintegrated in 500 μL of 5% Trichloroacetic acid (TCA) in UPW using steel balls and a minibeadbeater™ (Biospec, USA). After 48 hours of incubation at room temperature ...
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bioRxiv - Bioengineering 2022Quote: ... and each was disintegrated in 500 μl of 5% trichloroacetic acid (TCA) in UPW using steel balls and a Mini-BeadBeater™ (Biospec, USA). After 48 hours of incubation at room temperature ...
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bioRxiv - Neuroscience 2022Quote: The same frozen hippocampal and somatosensory cortex samples used for proteomics were cryogenically pulverized (cryopulverization) using a 12-well biopulverizer (BioSpec Products, OK USA Part number 59012MS) according to the manufacturer’s instructions ...