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• Host cell-specific metabolism of linoleic acid controls Toxoplasma gondii growth in cell culture

  Nicole D. Hryckowian, et al., bioRxiv - Microbiology 2024

  Quote: ... Fatty acids were purchased from Nu-Chek Prep (linoleic acid ...
• CryoRhodopsins: a comprehensive characterization of a group of microbial rhodopsins from cold environments

  G.H.U. Lamm, et al., bioRxiv - Biophysics 2024

  Quote: ... in the crystallization buffer was mixed with octyl-β-D-Glucopyranosid (OG, Glycon) and then with the premelted at 42°C monoolein (MO, Nu-Chek Prep) in a 2:1 ratio (protein/OG:lipid ...
• Elongation capacity of polyunsaturated fatty acids in the annelid Platynereis dumerilii

  Marc Ramos-Llorens, et al., bioRxiv - Biochemistry 2024

  Quote: ... All FA substrates (>98–99% pure) used for the functional characterisation assays were obtained from Nu-Chek Prep, Inc ...
• Disruption of liquid/liquid phase separation in asymmetric GUVs prepared by hemifusion

  Kristen B. Kennison-Cook, Frederick A. Heberle, bioRxiv - Biophysics 2024

  Quote: ... Cholesterol (Chol) was purchased from Nu-Chek Prep (Elysian, MN) as a dry powder and used as supplied ...
• Mathematical model of intestinal lipolysis of a long-chain triglyceride

  Oljora Rezhdo, et al., bioRxiv - Bioengineering 2024

  Quote: ... Diolein (DO, P/N: D-236-N9-Z) was purchased from Nu-Chek Prep Inc. ...
• Colorectal ALOX15 as a host factor determinant of EPA and DHA effects on colorectal carcinogenesis

  Xiangsheng Zuo, et al., bioRxiv - Cancer Biology 2024

  Quote: ... 1% EPA-EE diet (EPA-EE: catalog# U-99-E, NU-CHEK PREP, INC; the diet: TD.160449, Envigo), 1% EPA-TG diet (EPA-TG ...
• Colorectal ALOX15 as a host factor determinant of EPA and DHA effects on colorectal carcinogenesis

  Xiangsheng Zuo, et al., bioRxiv - Cancer Biology 2024

  Quote: ... 1% DHA-EE diet (DHA-EE: catalog# U-84-E, NU-CHEK PREP, INC; the diet: TD.160450, Envigo), or 1% DHA-TG diet (DHA-TG ...
• Targeting SCD triggers lipotoxicity of cancer cells and enhances anti-tumor immunity in breast cancer brain metastasis mouse models

  Alessandro Sammarco, et al., bioRxiv - Cancer Biology 2024

  Quote: ... Samples were prepared alongside internal standard curve samples made up of FAMEs (Nu-Chek Prep). Isotopomer spectral analysis (ISA ...
• FABP4-mediated lipid accumulation and lipolysis in tumor associated macrophages promote breast cancer metastasis

  Matthew Yorek, et al., bioRxiv - Immunology 2024

  Quote: ... all FFAs used in the current study were purchased from Nu-Chek Prep (MN). PA (5 mM) ...
• Enzymes enabling the biosynthesis of various C₂₀ polyunsaturated fatty acids in a sea urchin Hemicentrotus pulcherrimus

  Yingying Peng, Yutaka Haga, Naoki Kabeya, bioRxiv - Biochemistry 2024

  Quote: ... All fatty acid substrates were purchased from Nu-Chek Prep, Inc ...
• Halogenated cholesterol alters the phase behavior of ternary lipid membranes

  Deeksha Mehta, et al., bioRxiv - Biophysics 2024

  Quote: ... Cholesterol was purchased from Nu-Chek Prep (Elysian, MN). The fluorescent dyes 1,2-dioleoyl-3-[16-N-(Lissamine rhodamine B sulfonyl ...
• Fatty acid desaturation guides cellular decisions between ferroptosis and cellular senescence

  Chisaka Kuehnemann, et al., bioRxiv - Cell Biology 2024

  Quote: ... Peaks of interest were identified by comparison with authentic fatty acid standards (Nu-Chek Prep, Inc. MN) and expressed both as absolute concentration (μg/mg weight ...
• Colorectal ALOX15 as a host factor determinant of EPA and DHA effects on colorectal carcinogenesis

  Xiangsheng Zuo, et al., bioRxiv - Cancer Biology 2024

  Quote: ... or 1% DHA-TG diet (DHA-TG: catalog# T-310, NU-CHEK PREP, INC; the diet: TD.160452, Envigo) (diet details described in Supplementary Table 6 ...
• Colorectal ALOX15 as a host factor determinant of EPA and DHA effects on colorectal carcinogenesis

  Xiangsheng Zuo, et al., bioRxiv - Cancer Biology 2024

  Quote: ... 1% EPA-TG diet (EPA-TG: catalog# T-325, NU-CHEK PREP, INC; the diet: TD.160151, Envigo), 1% DHA-EE diet (DHA-EE ...
• Neuronal lipid droplets play a conserved and sex-biased role in maintaining whole-body energy homeostasis

  Romane Manceau, et al., bioRxiv - Neuroscience 2024

  Quote: ... 10 mM glucose and 0.25 mM oleate (Nu-chek Prep, #S-1120) in 0.27% bovine serum albumin (BSA ...
• Targeting de novo lipogenesis improves gemcitabine efficacy in pancreatic ductal adenocarcinoma

  Sarah E Hancock, et al., bioRxiv - Cancer Biology 2024

  Quote: ... All lipid internal standards were purchased from Merck Life Sciences except diacylglycerol 15:0/15:0 (Nu-Chek Prep, Elysian, MN, USA) and d5-triacylglycerol 16:0/16:0/16:0 (C/D/N Isotopes ...
• FAF2 is a bifunctional regulator of peroxisomal homeostasis and saturated lipid responses

  Choah Kim, et al., bioRxiv - Cell Biology 2024

  Quote: FFAs obtained from Nu-Chek Prep, Inc ...
• Elucidating the Impact of Bacterial Lipases, Human Serum Albumin, and FASII Inhibition on the Utilization of Exogenous Fatty Acids by Staphylococcus aureus

  Emily L. Pruitt, et al., bioRxiv - Microbiology 2023

  Quote: ... and arachidonic acid (20:4) (Nu-Chek Prep, Inc., Elysian, MN), each at a final concentration of 100 µM ...
• Elucidating the Impact of Bacterial Lipases, Human Serum Albumin, and FASII Inhibition on the Utilization of Exogenous Fatty Acids by Staphylococcus aureus

  Emily L. Pruitt, et al., bioRxiv - Microbiology 2023

  Quote: ... and C20:4 (Nu-Chek Prep, Inc., Elysian, MN) in ethanol each at 100 μM in TSB ...
• Elucidating the Impact of Bacterial Lipases, Human Serum Albumin, and FASII Inhibition on the Utilization of Exogenous Fatty Acids by Staphylococcus aureus

  Emily L. Pruitt, et al., bioRxiv - Microbiology 2023

  Quote: ... and arachidonic acid (20:4) (Nu-Chek Prep, Inc., Elysian, MN), each at a final concentration of 100 µM ...
• Seasonal and genetic effects on lipid profiles of juvenile Atlantic salmon

  Andrew H. House, et al., bioRxiv - Evolutionary Biology 2023

  Quote: ... Internal lipid standards TG 14:0/14:0/14:0 (NU-CHEK PREP), PC 14:1/14:1 and PE 14:0/14:0 (Avanti Polar Lipids ...
• FALCON systematically interrogates free fatty acid biology and identifies a novel mediator of lipotoxicity

  Nicolas Wieder, et al., bioRxiv - Cell Biology 2023

  Quote: ... Representative FFAs were ordered from Nu-Chek Prep, Inc. ...
• Adipocytes regulate fibroblast function, and their loss contributes to fibroblast dysfunction in inflammatory diseases

  Heather J. Faust, et al., bioRxiv - Cell Biology 2023

  Quote: ... Arachidonic acid (AA, Cat# U-71-A) was from Nu-Chek Prep. Hydrocortisone-d4 (cortisol-d4 ...
• Adipocytes regulate fibroblast function, and their loss contributes to fibroblast dysfunction in inflammatory diseases

  Heather J. Faust, et al., bioRxiv - Cell Biology 2023

  Quote: ... Arachidonic acid (AA, Cat# U-71- A) was purchased from Nu-Chek Prep. Triacylglycerol (TAG ...
• Comparative omics reveals unanticipated metabolic rearrangements in a high-oil mutant of plastid acetyl-CoA carboxylase

  Amr Kataya, et al., bioRxiv - Plant Biology 2023

  Quote: ... as well as 40 mg tripentadecanoin (15:0; Nu-chek Prep, Elysian, MN, USA) as an internal standard in a 10 cm x 1 cm glass tube ...
• Tld1 is a novel regulator of triglyceride lipolysis that demarcates a lipid droplet subpopulation

  Natalie Ortiz Speer, et al., bioRxiv - Cell Biology 2023

  Quote: ... Each plate was spotted with a neutral lipid reference standard mixture (Cat # 18-5C; Nu-Chek Prep). The standard was prepared in chloroform to a final concentration of 10 mg/ml and diluted to 1µg/µL before loading onto plate ...
• A novel subgroup of light-driven sodium pumps with an additional Schiff base counterion

  E. Podoliak, et al., bioRxiv - Biophysics 2023

  Quote: ... the solubilized protein (60 mg/ml) in the crystallization buffer was mixed with premelted at 42°C monoolein (MO, Nu-Chek Prep) in a 3:2 ratio (lipid:protein ...
• Adipocyte autophagy limits gut inflammation by controlling oxylipin levels

  Felix Clemens Richter, et al., bioRxiv - Immunology 2022

  Quote: ... FAMEs were identified routinely by comparing retention times of peaks with those of G411 FA standards (Nu-Chek Prep Inc). TotalChrom software (Perkin-Elmer ...
• Structural basis for receptor selectivity and inverse agonism in S1P₅ receptors

  Elizaveta Lyapina, et al., bioRxiv - Biophysics 2022

  Quote: ... made of monoolein (Nu-Chek Prep) supplemented with 10 %w/w cholesterol (Affymetrix) ...
• Remodeling of gene regulatory networks underlying thermogenic stimuli-induced adipose beiging

  Seoyeon Lee, et al., bioRxiv - Genomics 2022

  Quote: ... An equal weight FAME mixture (462A; Nu-Chek Prep, Inc.) was used to calculate response factors ...
• Overlapping local and systemic defense induced by an oomycete fatty acid MAMP and brown seaweed extract in tomato

  Domonique C. Lewis, et al., bioRxiv - Plant Biology 2022

  Quote: Fatty acid sodium salts (Na-FA; Nu-Chek Prep, Elysian, MN) were prepared and stored as previously described (Dye ...
• The oomycete MAMP, arachidonic acid, and an Ascophyllum nodosum-derived plant biostimulant induce defense metabolome remodeling in tomato

  Domonique C. Lewis, et al., bioRxiv - Plant Biology 2022

  Quote: Fatty acid sodium salts (Na-FA; Nu-Chek Prep, Elysian, MN) were prepared and stored as previously described (Dye et al ...
• Mediator subunit MDT-15 promotes expression of propionic acid breakdown genes to prevent embryonic lethality in Caenorhabditis elegans

  Grace Ying Shyen Goh, et al., bioRxiv - Genetics 2022

  Quote: ... and PUFAs (mix of fatty acid sodium salts: 150 μM C18:2, S-1127; 150 μM C20:5, S-1144, Nu-Chek Prep). E ...
• Structural insights into HIV-1 polyanion-dependent capsid lattice formation revealed by single particle cryo-EM

  Carolyn M. Highland, et al., bioRxiv - Biochemistry 2022

  Quote: ... Cholesterol was purchased from Nu-Chek Prep (Elysian, MN), and cholesterol stock solutions were prepared by standard gravimetric procedures to within 0.2% error ...
• Cardiac dysfunction affects eye development and vision by reducing supply of lipids in fish

  Elin Sørhus, et al., bioRxiv - Molecular Biology 2021

  Quote: Fatty acids (as 51 FAMEs) were identified by comparing retention times with a FAME standard (GLC-463 from Nu-Chek Prep. Elysian, MN, USA). Retention index maps and mass spectral libraries (GC-MS ...
• Pkd1 mutation has no apparent effects on peroxisome structure or lipid metabolism

  Takeshi Terabayashi, et al., bioRxiv - Genetics 2021

  Quote: ... The 11 standard stocks were prepared by serial dilution of GLC 401 (Nu-Chek Prep, MN) in methanol and were kept at 4°C ...
• Dietary Vitamin B₁₂ reduces amyloid-β proteotoxicity by alleviating oxidative stress and mitochondrial dysfunction

  Andy B. Lam, Kirsten Kervin, Jessica E. Tanis, bioRxiv - Cell Biology 2021

  Quote: ... 0.3mM sodium homogamma linolenate solution (Nu-Chek Prep), 148nM methylcobalamin (Millipore Sigma) ...
• The AdcACB/AdcAII system is Essential for Zinc Homeostasis and an Important Contributor of Enterococcus faecalis Virulence

  Ling Ning Lam, et al., bioRxiv - Microbiology 2021

  Quote: ... Authentic FAME standards were prepared as a set of dilutions 0.01 – 5.0 µg ml-1 (GLC-461 standard mix, Nu-Chek Prep) spiked with US-108N ISTD for FAME quantitation ...
• Single-cell Chromatin Accessibility and Lipid Profiling Reveals a Metabolic Shift in Adipocytes Induced by Bariatric Surgery

  Blaine Harlan, et al., bioRxiv - Genomics 2021

  Quote: ... An equal weight FAME mixture (462A; Nu-Chek Prep, Inc.) was used to calculate response factors daily ...
• Viral channelrhodopsins: calcium-dependent Na⁺/K⁺ selective light-gated channels

  D. Zabelskii, et al., bioRxiv - Biophysics 2020

  Quote: ... or monopalmitolein (MP, Nu-Chek Prep) in 2:3 ratio (lipid:protein ...
• Viral channelrhodopsins: calcium-dependent Na⁺/K⁺ selective light-gated channels

  D. Zabelskii, et al., bioRxiv - Biophysics 2020

  Quote: ... the solubilized protein (40 mg/ml) in the crystallization buffer was mixed with premelted at 50°C monoolein (MO, Nu-Chek Prep) or monopalmitolein (MP ...
• Molecular mechanism of light-driven sodium pumping

  K. Kovalev, et al., bioRxiv - Biophysics 2020

  Quote: ... The solubilized protein in the crystallization buffer was added to the monooleoyl-formed lipidic phase (Nu-Chek Prep, USA). The best crystals were obtained using the protein concentration of 25 mg/ml ...
• Inhibition of CMP-sialic acid transport by endogenous 5-methyl CMP

  Shivani Ahuja, et al., bioRxiv - Biochemistry 2020

  Quote: ... the protein was mixed 2:3 with monoolein (Nu-Chek Prep) and then 70 nl of this material was deposited on a glass slide (Molecular Dimensions) ...
• Nrf2 activator-encapsulating polymeric nanoparticles and LDL-like nanoparticles target atherosclerotic plaque

  Sophie Maiocchi, et al., bioRxiv - Bioengineering 2020

  Quote: ... and unesterified oleic acid (Nu-chek Prep, Inc) into LDL was performed by the reconstitution method as described previously26.
• Exogenous Nitro-Oleic Acid inhibits primary root growth by reducing the mitosis in the meristem in Arabidopsis thaliana

  Luciano M. Di Fino, et al., bioRxiv - Plant Biology 2020

  Quote: OA was purchased from Nu-Chek Prep (Elysian, MN). NO2-OA and biotinylated NO2-OA were synthesized and purified as previously described (Woodcock el at. ...
• Structural insights into the mechanism of rhodopsin phosphodiesterase

  Tatsuya Ikuta, et al., bioRxiv - Biophysics 2020

  Quote: The purified protein was concentrated to 10 mg /mL and mixed with monoolein (Nu-Chek Prep) in a 2:3 protein to lipid ratio (w/w) ...
• Demonstration of electron diffraction from membrane protein crystals grown in a lipidic mesophase after lamella preparation by focused ion beam milling at cryogenic temperatures

  Vitaly Polovinkin, et al., bioRxiv - Biophysics 2020

  Quote: ... and crystallization of BR (from Halobacterium salinarum S9 cells) in the lipidic cubic phase of monoolein (Nu-Chek Prep) were carried out as described in (Gordeliy et al. ...
• Stearoyl-CoA desaturase mediated monounsaturated fatty acid availability supports humoral immunity

  Xian Zhou, et al., bioRxiv - Immunology 2020

  Quote: ... stearic acid (NU-CHEK PREP, INC) were conjugated with fatty acid free BSA (Sigma-Aldrich ...
• Stearoyl-CoA desaturase mediated monounsaturated fatty acid availability supports humoral immunity

  Xian Zhou, et al., bioRxiv - Immunology 2020

  Quote: ... palmitic acid (NU-CHEK PREP, INC), stearic acid (NU-CHEK PREP ...
• Stearoyl-CoA desaturase mediated monounsaturated fatty acid availability supports humoral immunity

  Xian Zhou, et al., bioRxiv - Immunology 2020

  Quote: ... Palmitoleic acid (NU-CHEK PREP, INC), palmitic acid (NU-CHEK PREP ...