Labshake search
Citations for PacBio :
201 - 250 of 292 citations for ssc mir 299 Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... SMRTbell libraries of pooled amplicons (up to 29 samples per library) were prepared using SMRTbell Template Prep Kit 1.0 (PAcbio) and Sequel chemistry v3 and sequenced on a PacBio Sequel SMRT cell with a 10 hr ...
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bioRxiv - Microbiology 2021Quote: ... The genomic DNA was sheared to approximately 8-14 kb long fragments and a library was generated with the SMRTbell Express Template Prep Kit 2.0 (PacBio) without size selection ...
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bioRxiv - Genomics 2021Quote: ... and a SMRTbell library was prepared using the SMRT bell Template Prep Kit 1.0-SPv3 (Pacbio, 100-991-900). DNA quantification was performed using a Qubit fluorometer (Thermo Fisher ...
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bioRxiv - Genomics 2021Quote: ... Long-read DNA libraries were constructed using the SMRTbell Express Template Prep Kit 2.0 (PacBio, Menlo Park, CA, USA) and sequenced on SMRT cells (1M v3 LR ...
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bioRxiv - Genomics 2021Quote: The amplicon library was created using the SMRTbell Express Template Prep Kit 2.0 (PacBio, Cat. No. 100-938-900) as per manufacturer’s instructions ...
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bioRxiv - Genomics 2021Quote: ... PacBio SMRTbell libraries (∼20kb) were constructed using the SMRTbell Express Template Prep Kit 2.0 (PacBio, Menlo Park, CA, USA), following the manufacturer’s protocol ...
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bioRxiv - Genomics 2023Quote: ... The library preparation procedure is described in the protocol “Preparing HiFi Libraries from Low DNA Input Using SMRTbell Express Template Prep Kit 2.0” from PacBio. The SMRTbell library sizes and profiles were evaluated using the Agilent DNA 12000 kit on the Bioanalyzer system ...
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bioRxiv - Genomics 2023Quote: ... we sheared 10 µg of HMW DNA to an average fragment size of of 24 kb and prepared two independent non-barcoded HiFi SMRTbell libraries using SMRTbell Express Template Prep Kit 2.0 (PacBio). We pooled the two libraries and performed size-selection with a BluePippin (SageScience ...
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bioRxiv - Genomics 2023Quote: ... HMW DNA was used to generate PacBio HiFi libraries via the Template Prep Kit v1 (PacBio, 100-259-100) or SMRTbell Express Template Prep Kit v2 (PacBio ...
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bioRxiv - Genomics 2022Quote: ... The long-read library was then sequenced on the PacBio Sequel IIe system in CLR mode using the Sequel II Binding Kit 2.2 (PacBio). The short-read library with an insert size of 350 bp was sequenced on the NovaSeq6000 platform (Illumina ...
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bioRxiv - Genomics 2023Quote: ... libraries were constructed with an average insert size of 15kb using SMRTbell Express Template Prep Kit 2.0 (Pacific Biosciences, Menlo Park, USA) and sequenced by PacBio Sequel II ...
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bioRxiv - Evolutionary Biology 2023Quote: ... SMRTbell libraries (∼20kb) for PacBio Sequel were constructed using SMRTbell Template Prep Kit 1.0 (PacBio, Menlo Park, CA, USA) using the manufacturer’s recommended protocol ...
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bioRxiv - Plant Biology 2023Quote: Library preparation and PacBio sequencing was conducted by Eremid Genomic Services (Kannapolis, NC) using the SMRTbell prep kit 3.0 (PacBio), following the manufacturer’s instruction ...
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bioRxiv - Genetics 2023Quote: ... DNA was then cleaned and sheared to an average size of 19 kb before generating four HiFi libraries using SMRTbell template express kit 2.0 (PacBio). Final libraries were size-selected in the size range of 7-50kb and sequenced across 18 SMRT cells (see SI for details).
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bioRxiv - Evolutionary Biology 2023Quote: ... We prepared a PacBio HiFi library with the SMRTbell Express Template Prep Kit 2.0 (PacBio, Menlo Park, CA, USA). The library was sequenced on a PacBio Sequel II instrument on an 8M SMRT cell in CCS mode with a 30-hour movie time ...
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bioRxiv - Genomics 2024Quote: ... a SMRT bell library was constructed using the SMRTbell® prep kit 3.0 (PacBio Ref. No. 102-141-700), following the manufacturer’s instructions ...
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bioRxiv - Genomics 2024Quote: ... The resulting cDNA was used as input for the MAS-Seq for 10x Single Cell 3’ kit by PacBio.
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bioRxiv - Genomics 2024Quote: A SMRTbell library was then constructed with the SMRTbell® prep kit 3.0 (PacBio Ref. No. 102-141-700), following the manufacturer’s protocol ...
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bioRxiv - Genomics 2024Quote: ... and purification of polymerase-bound SMRTbell complexes were performed using the Revio polymerase kit (PacBio, Menlo Park, CA, USA). Loading concentration for sequencing was 225 pM ...
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bioRxiv - Genetics 2024Quote: ... Fragments smaller than 40 kb were depleted from these samples using the SRE XL kit (PacBio, SKU102-208-400) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2024Quote: ... The final library was prepared with the Sequel® II binding kit 3.2 (PacBio Ref. No. 102-194-100) and was loaded with the diffusion loading mode with the on-plate concentration set at 90 pM on the Pacific Biosciences SEQUEL IIe System ...
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bioRxiv - Genomics 2021Quote: A long-read sequence library was prepared using the SMRTbell Express Template Prep Kit 1.0 (PacBio, Menlo Park, CA, USA). The size selection of the library was performed by BluePippin (Sage Science ...
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bioRxiv - Microbiology 2021Quote: ... to 15 kb and size-selected using the Blue Pippin (Sage Science, USA) and prepared for sequencing using SMRTbell Express Template Preparation Kit 1.0 (PacBio, USA) according to manufacturers’ instructions ...
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bioRxiv - Genomics 2022Quote: ... Sequencing libraries were prepared for each species using the SMRTbell Express Template Prep Kit 2.0 (PacBio, Menlo Park, CA, USA) and following the ultra-low protocol ...
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bioRxiv - Genetics 2019Quote: 100-150ng of total RNA per reaction was reverse transcribed using the Clontech SMARTer cDNA synthesis kit and 12 sample specific barcoded oligo dT (with PacBio 16mer barcode sequences ...
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bioRxiv - Genomics 2020Quote: ... The two fractions were recombined at equimolar quantities and library preparation performed using SMRTbell Template Prep Kit v1.0 (PacBio, USA). Sequencing was performed on the PacBio Sequel 1M SMRT cell ...
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bioRxiv - Plant Biology 2022Quote: ... and a HiFi SMRTbell library was constructed with the SMRTbell Express Template Prep Kit 2.0 (PacBio, Menlo Park, CA, USA). The library DNA was fractionated using a BluePippin (Sage Science ...
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bioRxiv - Genomics 2022Quote: ... A linked-read sequencing library was prepared using the 10X Genomics Chromium Genomic Kit (from the same DNA extraction as used for PacBio sequencing ...
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bioRxiv - Molecular Biology 2023Quote: High molecular weight DNA was extracted from frozen pellets (1 million cells) using the Nanobind HMW DNA Extraction kit (PacBio) following the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2023Quote: ... Libraries were prepared and multiplexed with the PacBio SMRTbell® Express Template Prep Kit 2.0 (PacBio, Menlo Park, CA, USA) according to the published protocol (19) ...
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bioRxiv - Genomics 2023Quote: ... DNA was pooled and sequenced at the Australian Genome Research Facility (Brisbane, Queensland, Australia) using a SMRTbell® prep kit 3.0 (PacBio), and circular consensus sequencing (CCS ...
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bioRxiv - Genomics 2023Quote: ... Extractions were performed using the Nanobind Tissue Big DNA kit following the manufacturer’s instructions (Pacific BioSciences - PacBio, Menlo Park, CA). For song sparrow ...
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bioRxiv - Genomics 2024Quote: ... DNA samples for Oxford Nanopore or PacBio long read sequencing were enriched for HMW DNA (> 25 kb) employing the Short Read Eliminator Kit (Circulomics/PacBio). Quality control was performed by determining A260/A280 and A260/A230 ratios on NanoDrop ...
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bioRxiv - Evolutionary Biology 2024Quote: ... High molecular weight DNA was extracted from blood cells embedded in agarose blocks using the Nanobind CBB kit (Circulomics/PacBio). Construction of PacBio SMRT-bell sequencing libraries and long read sequencing on PacBio Sequel II were conducted at the Roy J ...
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bioRxiv - Genomics 2024Quote: A final library preparation was performed with The Sequel® II binding kit 3.2 (PacBio Ref. No. 102-194-100) before sequencing ...
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bioRxiv - Molecular Biology 2024Quote: ... a long-read sequence library was prepared using the SMRTbell Express Template Prep Kit 2.0 (PacBio, Menlo Park, CA, USA) and sequenced using a PacBio Sequel II system (PacBio ...
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bioRxiv - Genomics 2021Quote: ... The size-selected DNA was prepared into a PacBio library using the SMRTbell® Express Template Preparation Kit 2.0 (PacBio, USA), then sequenced across two PacBio Sequel II 8M SMRT cells (PN:101-389-001) ...
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bioRxiv - Genomics 2021Quote: ... Sequencing libraries for each sample were created using the SMRTbell Express Template Prep Kit 2.0 (PacBio, Cat. No. 100-938-900) per manufacturer’s instructions ...
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bioRxiv - Genetics 2023Quote: HiFi SMRTbell libraries were constructed according to the manufacturer’s manual by using SMRTbell Express Template Prep Kit 2.0 (PacBio, 100-938-900). For all samples ...
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bioRxiv - Genomics 2023Quote: ... The PacBio SMRTbell library (∼20kb) for PacBio Sequel was constructed using SMRTbell Template Prep Kit 1.0 (PacBio, Menlo Park, CA, USA) using the manufacturer recommended protocol ...
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bioRxiv - Evolutionary Biology 2023Quote: ... elegans female of each genotype (Ao, Io, oo), using the Nanobind® Tissue Big Extraction Kit (Cat. No. NB-900-701-01, Circulomics Inc. (PacBio), MD ...
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bioRxiv - Genomics 2024Quote: ... using settings 28/31 and used to generate PacBio HiFi libraries via the SMRTbell Prep Kit 3.0 (PacBio, 102-182-700). Size selection was performed with Pippin HT using a high-pass cutoff of 17 kbp (Sage Science ...
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bioRxiv - Microbiology 2024Quote: ... Samples with many short DNA fragments were further purified using either the Short Read Eliminator kit (PacBio, San Diego, CA, USA), or 0.4 - 0.5 x volume KAPA PURE SPRI beads (Roche ...
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bioRxiv - Plant Biology 2024Quote: ... the DNA was then size-selected using the Circulomics SRE kit v2.0 (SS-100-101-01, PacBio, Menlo Park, CA, USA). A concentration of 150 ng/µL of DNA was initially used ...
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bioRxiv - Immunology 2023Quote: ... PacBio SMRTbell amplicon libraries for PacBio Sequel were constructed using SMRTbell Express Template Prep Kit 2.0 (PacBio, Menlo Park, CA, USA) using the manufacturer recommended protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... and Germline DNA from blood with the Nanobind CBB kit (Circulomics NB-900-001-01, now PacBio SKU 102-301-900). After extraction ...
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bioRxiv - Microbiology 2019Quote: ... The long-read libraries were prepared with a SMRTbell library using a SMRTbell template prep kit 1.0 (PacBio, Menlo Park, CA, USA) with barcoding adapters according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... eluted in 37 μl EB (10 mM TrisHCl pH 8.0) and processed using SMRTBellTM Template Prep kit 1.0 (PacBio Part# 100-259-100) to SMRTbell libraries ...
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bioRxiv - Genomics 2022Quote: ... The PacBio SMRTbell library (~20kb) for PacBio Sequel was constructed using SMRTbell Express Template Prep Kit 2.0 (PacBio, Menlo Park, CA, USA) using the manufacturer recommended protocol ...
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bioRxiv - Genomics 2023Quote: The PacBio SMRTbell library (∼20kb) for PacBio Sequel was constructed using SMRTbell Express Template Prep Kit 2.0 (PacBio, Menlo Park, CA, USA) using the manufacturer recommended protocol ...