Labshake search
Citations for PacBio :
251 - 293 of 293 citations for rno mir 22 Real Time RT PCR Detection and U6 Calibration Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2023Quote: ... The DNA was then selected for high molecular weight DNA using the PacBio Short Read Eliminator kit (PacBio, Menlo Park, CA, USA). Concentration was assessed using the dsDNA BR assay on a Qubit fluorometer (Thermo Fisher Scientific ...
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bioRxiv - Genomics 2023Quote: ... a SMRTbell DNA library was constructed according to the Pacbio HiFi SMRTbell protocol using SMRTbell Express Template Prep Kit 3.0 (Pacbio, 102-182-700). After BluePippin size selection (Sage Science ...
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bioRxiv - Genetics 2023Quote: ... UW then prepared Iso-seq libraries from this cDNA using a Iso-Seq Express SMRTbell library prep kit (PacBio #100-938-900). The resulting iso-seq libraries were then sequenced by UW on a PacBio Sequel II system using an 8M SMRT cell (v2.1 chemistry) ...
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bioRxiv - Genomics 2023Quote: The PacBio SMRTbell library (∼20kb) for PacBio Sequel was constructed using SMRTbell Express Template Prep Kit 2.0 (PacBio, Menlo Park, CA, USA) using the manufacturer recommended protocol ...
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bioRxiv - Evolutionary Biology 2023Quote: ... The PacBio SMRTbell library (∼20kb) for PacBio Sequel was constructed using SMRTbell Express Template Prep Kit 2.0 (PacBio, Menlo Park, CA, USA) using the manufacturer recommended protocol ...
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bioRxiv - Genomics 2024Quote: The final library preparation for sequencing was performed with The Sequel® II binding kit 3.2 (PacBio Ref. No. 102-194-100). In brief ...
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bioRxiv - Genomics 2024Quote: ... The PacBio SMRTbell library (∼20kb) for PacBio Sequel was constructed using SMRTbell Express Template Prep Kit 2.0 (PacBio, Menlo Park, CA, USA) using the manufacturer-recommended protocol ...
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bioRxiv - Evolutionary Biology 2024Quote: ... The PacBio SMRTbell library (∼20kb) for PacBio Sequel was constructed using SMRTbell Express Template Prep Kit 2.0 (PacBio, Menlo Park, CA, USA) using the manufacturer recommended protocol ...
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bioRxiv - Genomics 2023Quote: HMW DNA from 1.5 x 106 cells was used to generate PacBio HiFi libraries with the SMRTbell express template prep kit 2.0 (PacBio 100-938-900). Size selection was performed with Megaruptor 2 (Diagenode ...
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bioRxiv - Evolutionary Biology 2024Quote: ... The PacBio SMRTbell library (∼20kb) for PacBio Sequel was constructed using SMRTbell Express Template Prep Kit 2.0 (PacBio, Menlo Park, CA, USA) using the manufacturer’s recommended protocol ...
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bioRxiv - Genomics 2024Quote: ... The purified linearized library was used to prepare a PacBio sequencing library using a SMRTbell prep kit 3.0 (PacBio #102-141-700), following the manufacturer’s recommended protocol (PacBio Protocol #102-166-600 REV02 ...
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bioRxiv - Genomics 2024Quote: ... The final preparation of the library was performed using the Sequel® II binding kit 3.2 (PacBio Ref. No. 102-194-100). In brief ...
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bioRxiv - Genetics 2019Quote: ... Bound SMRTbell libraries were loaded onto SMRT cells using standard MagBead protocols and the MagBead Buffer Kit v2.0 (PacBio Cat#100-642-800). The standard MagBead sequencing protocol was followed with the DNA Sequencing Kit 4.0 v2 (PacBio Cat#100-612-400 ...
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bioRxiv - Genetics 2019Quote: ... SMRTbell libraries were annealed and bound to P6 DNA polymerase for sequencing using the DNA/Polymerase Binding Kit P6 v2.0 (PacBio Cat#100-372-700). Bound SMRTbell libraries were loaded onto SMRT cells using standard MagBead protocols and the MagBead Buffer Kit v2.0 (PacBio Cat#100-642-800) ...
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bioRxiv - Microbiology 2021Quote: ... Sheared DNA was treated with exonuclease to remove single-stranded ends and DNA damage repair mix followed by end repair and ligation of barcoded blunt adapters using SMRTbell Template Prep Kit 2.0 (PacBio, Menlo Park, CA, USA). Libraries were purified with AMPure PB beads (Beckman Coulter ...
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bioRxiv - Genomics 2020Quote: A long-read sequence library was constructed using an SMRTbell Express Template Prep Kit 2.0 and sequenced on SMRT cells (1M v3 LR) in a PacBio Sequel system (PacBio, Menlo Park, CA, USA). The obtained reads (≥15 kb ...
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bioRxiv - Genomics 2021Quote: The manufacturer recommended protocol was used to generate a PacBio SMRTbell library (~20kb) for PacBio Sequel using SMRTbell Express Template Prep Kit 2.0 (PacBio, Menlo Park, CA, USA). The library was bound to polymerase using the Sequel II Binding Kit 2.0 (PacBio ...
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bioRxiv - Genomics 2022Quote: ... The sheared DNA was used for HiFi SMRTbell library preparation with the SMRTbell Express Template Prep Kit 2.0 (PacBio, Menlo Park, CA, USA). The resultant library was separated on BluePippin (Sage Science ...
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bioRxiv - Genomics 2022Quote: ... were added to ligation products following an adapted version of the PacBio Microbial Multiplexing PacBio protocol (Part Number 101-696-100 Version 07 (July 2020)) using the PacBio SMRTbell Express Template Prep Kit 2.0 (PacBio, PN: 100-938-900) as follows ...
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bioRxiv - Genomics 2022Quote: ... PacBio HiFi reads were generated by first constructing a ~20kb library using SMRTbell Express Template Prep Kit 2.0 (PacBio, Menlo Park, CA, USA) using the manufacturer’s recommended protocol ...
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bioRxiv - Microbiology 2022Quote: ... The sheared and purified DNA fragments were used as templates to prepare the SMRTbell library through SMRTbell template prep kit 1.0 (PacBio, Menlo Park, CA, USA), according to the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2023Quote: ... The PacBio SMRTbell library (~20kbp mean insert length) for PacBio Sequel was constructed using SMRTbell Express Template Prep Kit 2.0 (PacBio, Menlo Park, CA, USA) using the manufacturer recommended protocol ...
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bioRxiv - Genomics 2023Quote: ... Sheared DNA was treated with exonuclease to remove single stranded ends and DNA damage repair mix followed by end repair and ligation of barcoded blunt adapters using SMRTbell Template Prep Kit 2.0 (PacBio, Menlo Park, CA, USA). Libraries were purified with AMPure PB beads (Beckman Coulter ...
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bioRxiv - Genomics 2023Quote: ... The PacBio SMRTbell library (∼20 kbp mean insert length) for PacBio Sequel was constructed using SMRTbell Express Template Prep Kit 2.0 (PacBio, Menlo Park, CA, USA) using the manufacturer’s recommended protocol ...
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bioRxiv - Genomics 2023Quote: ... The PacBio SMRTbell library (∼20 kb) for PacBio Sequel was constructed using SMRTbell Express Template Prep Kit 2.0 (PacBio, Menlo Park, CA, USA) using the manufacturer recommended protocol ...
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bioRxiv - Evolutionary Biology 2023Quote: ... we used 80 ng of each library in the end-repair/ligation reaction using barcoded adaptors from the SMRTbell barcoded adapter complete prep kit -96 (PacBio # 100-514-900). We then pooled all the barcoded libraries to be multiplexed and purified the pooled library using the AMPure PB Kit (PacBio # 100-265-900) ...
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bioRxiv - Evolutionary Biology 2023Quote: ... we performed a DNA damage repair reaction followed by exonuclease treatment (ExoIII and ExoVII) using the SMRTbell DNA Damage Repair Kit (PacBio # 100-486-900) according manufacturer’s instruction ...
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bioRxiv - Evolutionary Biology 2023Quote: ... We then pooled all the barcoded libraries to be multiplexed and purified the pooled library using the AMPure PB Kit (PacBio # 100-265-900). We used a concentration of 0.6X of AMPure Beads and eluted the DNA in 37 µl of elution buffer ...
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bioRxiv - Genomics 2023Quote: ... The PacBio SMRTbell library (∼20kb) for PacBio Sequel was constructed using the SMRTbell Express Template Prep Kit 2.0 (PacBio, Menlo Park, CA, USA) following the manufacturer recommended protocol ...
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bioRxiv - Genomics 2023Quote: ... They constructed the PacBio SMRTbell library (∼20kb) for PacBio Sequel using SMRTbell Express Template Prep Kit V 2.0 (PacBio, Menlo Park, CA, USA) using the manufacturer recommended protocol ...
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bioRxiv - Genomics 2024Quote: ... A SMRTbell library was then prepared by following the protocol of the SMRTbell® prep kit 3.0 (PacBio Ref. No. 102-141-700). Briefly ...
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bioRxiv - Cancer Biology 2023Quote: ... All samples were depleted of short DNA fragments (less than 10kb long) with the short read eliminator (SRE) kit (Circulomics, SS-100-101-01; now PacBio 102-208-300) or the SRE XS kit (Circulomics SS-100-121-01 ...
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bioRxiv - Evolutionary Biology 2021Quote: ... DNA was extracted using the Qiagen kits Dneasy (for short-read sequencing; from approximately 5000-7000 rotifers) and GenomicTips 100 (for long read Pacbio sequencing; from >20000 rotifers), and RNA was extracted from freshly prepared biomass with Rneasy.
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bioRxiv - Genomics 2019Quote: ... The nine cDNAs were pooled in equimolar ratios and used to prepare a SMRTbell™ library using the PacBio Template Prep Kit (PacBio, Menlo Park, CA). The SMRTbell™ library was then sequenced across four Sequel v2 SMRT cells with polymerase 2.1 and chemistry 2.1 (P2.1C2.1).
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bioRxiv - Neuroscience 2020Quote: ... PacBio library preparation was performed with 500 ng of amplified cDNA using SMRTbell Express Template Prep Kit V2.0 (PacBio cat#PN: 100-938-900) to obtain Sequel II compatible library complex and was sequenced on a total 24 Sequel I SMRTcells with a run time of 10 hours and 20 Sequel II SMRTcells with a run time of 30 hours across samples and replicates.
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bioRxiv - Genomics 2020Quote: ... The three cDNAs were pooled in equimolar ratios and used to prepare a SMRTbell™ library using the PacBio Template Prep Kit (PacBio, Menlo Park, CA). The SMRTbell™ library was then sequenced on a Sequel v2 SMRT cell with polymerase 2.1 and chemistry 2.1 (P2.1C2.1 ...
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bioRxiv - Genomics 2023Quote: The HiFi SMRTbell library was constructed using the SMRTbell Express Template Prep Kit v2.0 (Pacific Biosciences -PacBio, Menlo Park, CA, Cat. #100-938-900) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2023Quote: ... a fraction of short fragments was removed from 9 μg of total DNA using the Short-Read Eliminator Kit XL (PacBio, SKU 102-208-400), according to the manufacturer’s recommendations ...
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bioRxiv - Genomics 2024Quote: High molecular weight genomic DNA was extracted from heart tissue according to the protocol of (Sambrook and Russell) for the first library and from lung tissue using the PacBio Nanobind Tissue Kit (PacBio, Menlo Park, CA, USA) for the second library ...
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bioRxiv - Plant Biology 2023Quote: ... Short read elimination was performed using the Circulomics Short Read Eliminator kit (formerly SS-100-101-01, now SKU 102-208-300; PacBio, Menlo Park, California, USA). The size selected DNA was quantified using a Qubit 1.0 Fluorometer (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2024Quote: Extraction of high molecular weight DNA from overnight cultures of CPs was accomplished using the Nanobind CBB kit (PacBio catalog no.102-301-900). Barcoded sequencing libraries were prepared using the SMRTbell prep kit 3.0 (PacBio catalog no.102-141-700 ...
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bioRxiv - Cancer Biology 2023Quote: Tumour genomic DNA was extracted from fresh-frozen tissue with the Nanobind tissue kit (Circulomics NB-900-701-01, now PacBio SKU 102-302-100) and Germline DNA from blood with the Nanobind CBB kit (Circulomics NB-900-001-01 ...
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bioRxiv - Genomics 2023Quote: The HiFi SMRTbell library was constructed using the SMRTbell Express Template Prep Kit v2.0 following the manufacturer’s protocols (Pacific Biosciences - PacBio, Menlo Park, CA; Cat. #100-938-900). HMW gDNA was sheared to a target DNA size distribution between 15-20 Kb and concentrated using 0.45X of AMPure PB beads (PacBio Cat ...