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Citations for PacBio :
1 - 50 of 335 citations for Onion Yellow Dwarf Virus OYDV PCR Kits since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: The mCherry-E3L integration sites in the purified HGT virus genomes were located by inverse PCR amplification and by PacBio sequencing ...
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bioRxiv - Genetics 2024Quote: ... and a PCR-free library was prepared using SMRTbell prep kit 3.0 (PacBio). Long-read whole-genome sequencing was done using a PacBio Revio system at an average coverage of approx ...
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bioRxiv - Neuroscience 2021Quote: ... The SMRT sequencing was performed on the PacBio Sequel at the Genomics Core at KU Leuven (Belgium) using the Clontech SMARTer PCR cDNA Synthesis Kit (recommended by Pacbio). Multiple parallel PCR reactions were set after the first strand synthesis employing the PrimeSTAR GXL DNA Polymerase kit ...
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bioRxiv - Molecular Biology 2024Quote: ... The purified ligation product was split into two reactions and enriched using 10-18 cycles of PCR using barcoded amplification oligos (IDT) and SMRTbell® gDNA Sample Amplification Kit (PacBio). Up to sixteen libraries were pooled in equimolar concentrations and the pooled libraries were size-selected using the 0.75% agarose gel cassettes with Marker S1 and High Pass protocol on the BluePippin (Sage Science) ...
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bioRxiv - Cancer Biology 2024Quote: ... PCR products were purified using AmpurePB beads (PacBio) according to manufacturer’s instructions and were then subjected to a second round (8 cycles ...
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bioRxiv - Microbiology 2021Quote: ... PCR products were purified using AMPure®PB beads (PacBio), following manufacturer’s guidelines ...
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bioRxiv - Microbiology 2022Quote: ... PCR product pools were purified using AMPure®PB beads (PacBio). Sequencing was performed at the Functional Genomic Center Zürich ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Using SMRTBell template Prep Kit 1.0 and SMRTbell Barcoded Adaptater kit 8A or 8B kits (PacBio), samples (1 µg ...
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bioRxiv - Bioengineering 2023Quote: ... 2 weeks later and subjected to large deletions detection (PCR and PacBio sequencing). Single cells were isolated from bulk edited cells into 96-well plates 2 weeks after electroporation and genotyped 2 weeks after isolation ...
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bioRxiv - Immunology 2024Quote: ... Pooled PCR products were sequenced on a 8M SMRT cell (PacBio Sequel II) by the Leiden Genome Technology Center.
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bioRxiv - Genomics 2024Quote: ... to reduce potential PCR biases of the amplification polymerases A/B supplied by PacBio, we prepared two further libraries using KOD Xtreme™ Hot Start DNA Polymerase C (Merck) ...
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bioRxiv - Genomics 2024Quote: ... The final Iso-Seq library was constructed following the PacBio Kinnex PCR protocol (PacBio) and sequenced on one Revio SMRT cell ...
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bioRxiv - Genomics 2020Quote: ... Sequencing was performed on a PacBio Sequel instrument using the Sequel® Binding Kit and Internal control Kit 3.0 and the Sequel® Sequencing Kit 3.0 (PacBio). Long read data was assembled using the HGAP4 assembler.
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bioRxiv - Genomics 2023Quote: ... and the Express Template Prep Kit v2 and SMRTbell Cleanup Kit v2 (PacBio). The library was size-selected on a PippinHT instrument (Sage Science ...
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bioRxiv - Microbiology 2021Quote: ... PCR chemicals and conditions (27 cycles) were performed as suggested by Pacific Biosciences (PacBio, 2020). PCR products were visualized on an 1 % (w/v ...
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bioRxiv - Microbiology 2021Quote: ... SMRTbell libraries were prepared using the SMRTbell Express Template Prep Kit 2.0 (PacBio kit). Long read data were assembled by canu 2.1.1(44) ...
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bioRxiv - Plant Biology 2021Quote: ... A Template Prep Kit (PacBio, USA) was used to remove failed ligation products ...
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bioRxiv - Developmental Biology 2023Quote: ... Iso-Seq Express Oligo Kit (PacBio) and SMRTbell Express Template Prep Kit 2.0 (PacBio) ...
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bioRxiv - Genomics 2024Quote: ... The Short Read Eliminator Kit (PacBio) was used to reduce the number of small DNA fragments according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2024Quote: ... using the Binding kit 2.2 (PacBio). Using SMRT Link (v10.1.0.119528 ...
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bioRxiv - Genomics 2024Quote: ... the DNA was used for CCS (circular consensus sequencing) library preparation using the SMRTbell Express Template Prep Kit 2.0 and Enzyme Cleanup Kit 1.0 (PacBio). Each library was barcoded using PacBio Barcoded Overhang Adapters ...
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bioRxiv - Genomics 2024Quote: ... using a Sequel II SMRT Cell 8M along with a Sequel II Binding Kit 3.2 and Sequel II Sequencing Kit 2.0 (PacBio). These library preparations and sequencings were performed at the National Institute of Genetics Japan (NIG ...
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bioRxiv - Genomics 2021Quote: ... Using SMRTBell template Prep Kit 1.0 (PacBio), a DNA and END damage repair step was performed on 5 μg of sample ...
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bioRxiv - Genomics 2023Quote: ... Using SMRTBell template Prep Kit 1.0 (PacBio), a DNA and END damage repair step was performed on 15µg of unshared sample ...
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bioRxiv - Cancer Biology 2022Quote: ... Sequencing Kit 2.0 (PacBio, 101-389-001) and Instrument Chemistry Bundle Version 11.0 were used ...
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bioRxiv - Genomics 2024Quote: ... The Short Read eliminator kit from PacBio was also used to increase average fragment size.
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bioRxiv - Microbiology 2024Quote: ... was extracted with the Circulomics kit (PacBio) according to the manufacturer protocol for gram-positive and gram-negative bacteria ...
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bioRxiv - Genomics 2023Quote: ... An Iso-Seq cDNA library was generated with the Iso-Seq Express Oligo Kit and the SMRTbell Express Template Prep Kit 2.0 (PacBio). The Iso-Seq library was sequenced using a single SMRTcell on a Sequel I at the D-BSSE (Basel ...
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bioRxiv - Genomics 2020Quote: ... HMW DNA was used to generate HiFi libraries via the SMRTbell Express Template Prep Kit v2 and SMRTbell Enzyme Clean Up kits (PacBio). Size selection was performed with SageELF (Sage Science) ...
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bioRxiv - Genomics 2023Quote: ... The sheared material was subjected to SMRTbell library preparation using the Express Template Prep Kit v2 and SMRTbell Cleanup Kit v2 (PacBio). After checking for size and quantity ...
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bioRxiv - Genomics 2024Quote: ... DNA was extracted using either the Nanobind Tissue Big DNA kit (cell line and brain) or the Nanobind HT 1ml blood kit (blood) (PacBio). For the cell line and blood samples ...
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bioRxiv - Genomics 2024Quote: ... The sheared material was processed for SMRTbell library preparation using the Express Template Prep Kit v2 and SMRTbell Cleanup Kit v2 (PacBio). After checking for size and quantity ...
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bioRxiv - Genomics 2024Quote: Total genomic DNA was extracted from cell pellets using Qiagen QIAamp DNA Micro Kit and the high molecular weight fraction was purified using a short read elimination kit (PacBio), which depletes the sample from all DNA fragments <10kb ...
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bioRxiv - Microbiology 2021Quote: ... using the Sequel II Sequencing Kit 2.0 (PacBio). Sequencing data quality were checked ...
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bioRxiv - Genetics 2021Quote: ... and the Express Library Prep Kit v2 (PacBio), size selecting on the SageELF platform (Sage Science ...
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bioRxiv - Plant Biology 2022Quote: ... and SMRTbell Express Template Prep Kit 2.0 (PacBio). Thereafter ...
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bioRxiv - Developmental Biology 2023Quote: ... and SMRTbell Express Template Prep Kit 2.0 (PacBio). Samples were sequenced using PacBio Sequel II 8M SMRT cell (version 2.1 ...
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bioRxiv - Genomics 2023Quote: ... using the Nanobind CBB DNA extraction kit (PacBio), according to the manufacturer’s instructions with modifications ...
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bioRxiv - Genetics 2022Quote: ... The SMRTBell® Template Prep Kit 1.0 (PacBio) was then used to prepare 14-kilobase libraries ...
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bioRxiv - Genomics 2024Quote: ... The SMRTbell® prep kit 3.0 from Pacbio was used to prepare the sequencing libraries ...
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bioRxiv - Genomics 2022Quote: ... Barcoded PacBio SMRTBell adapters from the PacBio 8A barcoded adapter kit (Barcoded Overhang Adapter Kit - 8A, PacBio PN: 101-628-400) were added to ligation products following an adapted version of the PacBio Microbial Multiplexing PacBio protocol (Part Number 101-696-100 Version 07 (July 2020) ...
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bioRxiv - Genomics 2024Quote: ... and long-read sequencing libraries were prepared using the SMRTbell Express Template Prep Kit v2 with a barcoded overhang adapter kit (PacBio, CA, USA) for multiplexing ...
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bioRxiv - Microbiology 2021Quote: ... using the Sequel II Sequencing Kit 1.0 (PacBio, USA) with the Sequel II SMRT Cell 8M (PacBio ...
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bioRxiv - Genomics 2023Quote: ... and Iso-Seq Express Oligo Kit (Pacific Biosciences (PacBio), CA ...
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bioRxiv - Genomics 2023Quote: ... or Circulomics Nanobind Tissue kit (Pacbio 102-302-100). The linked read library was prepared using the Chromium Genome Reagent Kit v2 following the protocol provided by 10x Genomics ...
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bioRxiv - Genomics 2023Quote: ... The Sequel® II Binding Kit 2.2 (PacBio, USA) was used to prepare the libraries for loading ...
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bioRxiv - Genomics 2023Quote: ... using the Sequel® II Sequencing Kit 2.0 (PacBio) on the Sequel® II Sequencer (PacBio).
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bioRxiv - Genomics 2022Quote: ... It’s recommended to use the barcode kit by PacBio to achieve the best sequencing result ...
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bioRxiv - Evolutionary Biology 2024Quote: ... the recommended SMRTbell Express Template Prep Kit 2.0 (PacBio) was used to prepare the libraries ...
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bioRxiv - Genomics 2024Quote: ... We used the Sequel II Binding Kit 2.0 (PacBio) to bind the library to polymerase and loaded libraries onto PacBio Sequel II ...