Labshake search
Citations for PacBio :
251 - 279 of 279 citations for Mouse Dachshund Homolog 1 DACH1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2022Quote: ... PacBio HiFi reads were generated by first constructing a ~20kb library using SMRTbell Express Template Prep Kit 2.0 (PacBio, Menlo Park, CA, USA) using the manufacturer’s recommended protocol ...
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bioRxiv - Microbiology 2022Quote: ... The sheared and purified DNA fragments were used as templates to prepare the SMRTbell library through SMRTbell template prep kit 1.0 (PacBio, Menlo Park, CA, USA), according to the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2023Quote: ... The PacBio SMRTbell library (~20kbp mean insert length) for PacBio Sequel was constructed using SMRTbell Express Template Prep Kit 2.0 (PacBio, Menlo Park, CA, USA) using the manufacturer recommended protocol ...
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bioRxiv - Genomics 2023Quote: ... Sheared DNA was treated with exonuclease to remove single stranded ends and DNA damage repair mix followed by end repair and ligation of barcoded blunt adapters using SMRTbell Template Prep Kit 2.0 (PacBio, Menlo Park, CA, USA). Libraries were purified with AMPure PB beads (Beckman Coulter ...
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bioRxiv - Genomics 2023Quote: ... The PacBio SMRTbell library (∼20 kbp mean insert length) for PacBio Sequel was constructed using SMRTbell Express Template Prep Kit 2.0 (PacBio, Menlo Park, CA, USA) using the manufacturer’s recommended protocol ...
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bioRxiv - Genomics 2023Quote: ... The PacBio SMRTbell library (∼20 kb) for PacBio Sequel was constructed using SMRTbell Express Template Prep Kit 2.0 (PacBio, Menlo Park, CA, USA) using the manufacturer recommended protocol ...
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bioRxiv - Evolutionary Biology 2023Quote: ... we used 80 ng of each library in the end-repair/ligation reaction using barcoded adaptors from the SMRTbell barcoded adapter complete prep kit -96 (PacBio # 100-514-900). We then pooled all the barcoded libraries to be multiplexed and purified the pooled library using the AMPure PB Kit (PacBio # 100-265-900) ...
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bioRxiv - Evolutionary Biology 2023Quote: ... we performed a DNA damage repair reaction followed by exonuclease treatment (ExoIII and ExoVII) using the SMRTbell DNA Damage Repair Kit (PacBio # 100-486-900) according manufacturer’s instruction ...
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bioRxiv - Evolutionary Biology 2023Quote: ... We then pooled all the barcoded libraries to be multiplexed and purified the pooled library using the AMPure PB Kit (PacBio # 100-265-900). We used a concentration of 0.6X of AMPure Beads and eluted the DNA in 37 µl of elution buffer ...
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bioRxiv - Genomics 2023Quote: ... The PacBio SMRTbell library (∼20kb) for PacBio Sequel was constructed using the SMRTbell Express Template Prep Kit 2.0 (PacBio, Menlo Park, CA, USA) following the manufacturer recommended protocol ...
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bioRxiv - Genomics 2023Quote: ... They constructed the PacBio SMRTbell library (∼20kb) for PacBio Sequel using SMRTbell Express Template Prep Kit V 2.0 (PacBio, Menlo Park, CA, USA) using the manufacturer recommended protocol ...
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bioRxiv - Genomics 2024Quote: ... A SMRTbell library was then prepared by following the protocol of the SMRTbell® prep kit 3.0 (PacBio Ref. No. 102-141-700). Briefly ...
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bioRxiv - Cancer Biology 2023Quote: ... All samples were depleted of short DNA fragments (less than 10kb long) with the short read eliminator (SRE) kit (Circulomics, SS-100-101-01; now PacBio 102-208-300) or the SRE XS kit (Circulomics SS-100-121-01 ...
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bioRxiv - Microbiology 2020Quote: Raw data were processed using the protocol RS_Readsofinsert.1 in SMRT Portal version 2.7 software (PacBio). Low-quality sequences were removed using the Quantitative Insights Into Microbial Ecology (QIIME ...
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bioRxiv - Evolutionary Biology 2021Quote: ... DNA was extracted using the Qiagen kits Dneasy (for short-read sequencing; from approximately 5000-7000 rotifers) and GenomicTips 100 (for long read Pacbio sequencing; from >20000 rotifers), and RNA was extracted from freshly prepared biomass with Rneasy.
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bioRxiv - Genomics 2019Quote: ... The nine cDNAs were pooled in equimolar ratios and used to prepare a SMRTbell™ library using the PacBio Template Prep Kit (PacBio, Menlo Park, CA). The SMRTbell™ library was then sequenced across four Sequel v2 SMRT cells with polymerase 2.1 and chemistry 2.1 (P2.1C2.1).
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bioRxiv - Neuroscience 2020Quote: ... PacBio library preparation was performed with 500 ng of amplified cDNA using SMRTbell Express Template Prep Kit V2.0 (PacBio cat#PN: 100-938-900) to obtain Sequel II compatible library complex and was sequenced on a total 24 Sequel I SMRTcells with a run time of 10 hours and 20 Sequel II SMRTcells with a run time of 30 hours across samples and replicates.
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bioRxiv - Genomics 2020Quote: ... The three cDNAs were pooled in equimolar ratios and used to prepare a SMRTbell™ library using the PacBio Template Prep Kit (PacBio, Menlo Park, CA). The SMRTbell™ library was then sequenced on a Sequel v2 SMRT cell with polymerase 2.1 and chemistry 2.1 (P2.1C2.1 ...
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bioRxiv - Genomics 2023Quote: The HiFi SMRTbell library was constructed using the SMRTbell Express Template Prep Kit v2.0 (Pacific Biosciences -PacBio, Menlo Park, CA, Cat. #100-938-900) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2023Quote: ... a fraction of short fragments was removed from 9 μg of total DNA using the Short-Read Eliminator Kit XL (PacBio, SKU 102-208-400), according to the manufacturer’s recommendations ...
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bioRxiv - Genomics 2024Quote: High molecular weight genomic DNA was extracted from heart tissue according to the protocol of (Sambrook and Russell) for the first library and from lung tissue using the PacBio Nanobind Tissue Kit (PacBio, Menlo Park, CA, USA) for the second library ...
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bioRxiv - Plant Biology 2023Quote: ... Short read elimination was performed using the Circulomics Short Read Eliminator kit (formerly SS-100-101-01, now SKU 102-208-300; PacBio, Menlo Park, California, USA). The size selected DNA was quantified using a Qubit 1.0 Fluorometer (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2024Quote: Extraction of high molecular weight DNA from overnight cultures of CPs was accomplished using the Nanobind CBB kit (PacBio catalog no.102-301-900). Barcoded sequencing libraries were prepared using the SMRTbell prep kit 3.0 (PacBio catalog no.102-141-700 ...
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bioRxiv - Cancer Biology 2023Quote: Tumour genomic DNA was extracted from fresh-frozen tissue with the Nanobind tissue kit (Circulomics NB-900-701-01, now PacBio SKU 102-302-100) and Germline DNA from blood with the Nanobind CBB kit (Circulomics NB-900-001-01 ...
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bioRxiv - Genomics 2023Quote: The HiFi SMRTbell library was constructed using the SMRTbell Express Template Prep Kit v2.0 following the manufacturer’s protocols (Pacific Biosciences - PacBio, Menlo Park, CA; Cat. #100-938-900). HMW gDNA was sheared to a target DNA size distribution between 15-20 Kb and concentrated using 0.45X of AMPure PB beads (PacBio Cat ...
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bioRxiv - Genomics 2022Quote: ... saligna CGN05327 (Supplemental Figure 1) was assembled using a ∼21-fold coverage of long-read data generated by PacBio Sequel technology (4,083,751 reads ...
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bioRxiv - Evolutionary Biology 2024Quote: ... We thus validated our haplotype block analysis by estimating the correspondence between our allele frequency-based inferred haplotypes and (1) sequences from PacBio HiFi-based long read assemblies of the 12 PSGs in five diploid and five tetraploid samples of A ...
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bioRxiv - Genomics 2023Quote: Sequencing data were obtained using two complementary long-read sequencing technologies for the assembly of hTERT RPE-1 cells: Pacific Biosciences (PacBio) High-Fidelity (HiFi ...
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bioRxiv - Microbiology 2020Quote: ... k236-1 and k273) were sequenced using the PacBio platform (The Norwegian Sequencing Centre; PacBio RSII platform using P5-C3 chemistry).