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Citations for PacBio :
151 - 200 of 270 citations for Human Ras Related Protein M Ras MRAS ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2023Quote: ... For 5µg of DNA an extra repair step with SMRTbell DAMAGE REPAIR KIT (PACBIO, 100-465-900) was performed before the one step DNA damage repair + END-repair + dA tail of double stranded DNA fragments and adapters ligation ...
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bioRxiv - Genomics 2023Quote: ... and then subjected to HiFi SMRTbell library preparation with the SMRTbell Express Template Prep Kit 2.0 (PacBio). The resultant library was separated on BluePippin (Sage Science ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Real-Time (SMRT) library using SMRTbell Express Template Prep Kit v2.0 (PacBio, CA; Cat. #100-938-900). We sheared HMW gDNA to a target DNA size distribution between 15 – 20 kb and concentrated it using 0.45X of AMPure PB beads (PacBio ...
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bioRxiv - Genomics 2024Quote: ... Further selection of high molecular weight DNA was performed using the Short Read Eliminator (SRE) kit (PacBio) according to the manufacturer’s guide ...
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bioRxiv - Genomics 2024Quote: ... the DNA went through a size selection step using a SRE Kit (PacBio, SKU-102-208-300) to remove fragments up to 25kb ...
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bioRxiv - Cell Biology 2024Quote: ... lucifugus fibroblast line was isolated using the Nanobind ®HMW DNA extraction kit for cultured cells (PacBio). M ...
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bioRxiv - Genomics 2020Quote: ... an SMRT sequence library was constructed with an SMRTbell Express Template Prep Kit (PacBio, Menlo Park, CA, USA) and sequenced on a PacBio Sequel system (PacBio) ...
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bioRxiv - Immunology 2020Quote: ... 5 ug of fragmented DNAs were converted to SMRTbell libraries using the SMRTbell Template Prep Kit 1.0 (PacBio). The libraries were size-selected (7-kb size cutoff ...
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bioRxiv - Plant Biology 2020Quote: ... PacBio SMRTbell libraries were constructed from the HMW DNA using the SMRTbell Express Template Prep Kit 2.0 following the manufacturer’s protocols (PacBio). A total of 24 Single-Molecule Real-Time (SMRT ...
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bioRxiv - Microbiology 2021Quote: ... The PacBio shotgun library was constructed following the manufacturer’s multiplexed bacterial protocol for the SMRTbell Express TPK 2.0 kit (PacBio). Briefly ...
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bioRxiv - Microbiology 2021Quote: ... A ready to sequence SMRT bell-Polymerase Complex was created using the Sequel II Binding Kit 2.1 and Internal Control 1.0 (PacBio) according to the manufacturer instruction ...
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bioRxiv - Genomics 2022Quote: Genomic DNA was subjected to HiFi SMRTbell library construction using the SMRTbell Express Template Prep Kit 2.0 (PacBio), according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2022Quote: ... Seventeen 1Mv3 SMRT cells were run on a Sequel I sequencer using the Binding Kit 1.0 and the Sequencing Chemistry version 1.0 (all from PacBio) for six hours per cell ...
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bioRxiv - Genetics 2023Quote: ... total RNA was converted into cDNA using a Iso-Seq Express cDNA generation kit (PacBio #101-737-500). UW then prepared Iso-seq libraries from this cDNA using a Iso-Seq Express SMRTbell library prep kit (PacBio #100-938-900) ...
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bioRxiv - Genomics 2023Quote: ... end-repair/A-tailing mix and ligated with overhang adapters using SMRTbell Express Template Prep Kit 2.0 (PacBio) and purified with AMPure PB Beads (PacBio) ...
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bioRxiv - Neuroscience 2023Quote: ... Library preparation of the amplified cDNA products was then performed using SMRTbell Template Prep Kit v1.0 (PacBio, USA). Sequencing was performed on the PacBio Sequel 1M SMRT cell ...
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bioRxiv - Genomics 2024Quote: ... A SMRTbell library was constructed using the SMRTbell® prep kit 3.0 (PacBio Ref. No. 102-141-700), following the manufacturer’s protocol ...
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bioRxiv - Genomics 2024Quote: ... a SMRTbell library was constructed using the SMRTbell® prep kit 3.0 (PacBio Ref. No. 102-141-700), following the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2024Quote: ... Two SMRTbell libraries were constructed with the SMRTbell® prep kit 3.0 (PacBio Ref. No. 102-141-700), following the manufacturer’s protocol ...
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bioRxiv - Evolutionary Biology 2024Quote: ... HMW gDNA for long-read sequencing was extracted using the Nanobind tissue kit (PacBio, Menlo Park, CA, USA) following the application note for isolation of HMW DNA from frozen tilapia testis tissue.
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bioRxiv - Genomics 2024Quote: A SMRTbell library was made using the SMRTbell® prep kit 3.0 (PacBio Ref. No. 102-141-700), following the provided protocol ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Binding of the library pools to the sequencing polymerase was completed using Sequel II Binding Kit v2.0 or 2.2 (PacBio). Calculations for primer to template and polymerase to template binding ratios were kept at default values ...
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bioRxiv - Genomics 2024Quote: Extracted DNA was size selected using the SRE XS kit (Pacbio US, Catalogue number: SKU 102-208-200) following manufacturer’s instructions to remove fragments < 10 kb in length.
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bioRxiv - Genomics 2021Quote: ... A sequence library for isoform sequencing (Iso-Seq) was prepared using the Iso-Seq Express Template Preparation Kit (PacBio) and sequenced on the PacBio Sequel system (PacBio) ...
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bioRxiv - Evolutionary Biology 2020Quote: ... USA) which were subsequently utilized to construct a library separately through the SMRTbellTM Template Prep Kit 1.0 (PacBio, USA). Libraries were sequenced by the PacBio Sequel (PacBio ...
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bioRxiv - Genomics 2020Quote: ... Sequencing libraries were constructed using the protocol “Preparing HiFi SMRTbell Libraries using SMRTbell Express Template Prep Kit 2.0” from PacBio. SMRTbells were size selected using 0.75% agarose 1-18kb protocol on SageELF (Sage Science ...
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bioRxiv - Neuroscience 2020Quote: ... SMRTbell libraries of pooled amplicons (up to 29 samples per library) were prepared using SMRTbell Template Prep Kit 1.0 (PAcbio) and Sequel chemistry v3 and sequenced on a PacBio Sequel SMRT cell with a 10 hr ...
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bioRxiv - Microbiology 2021Quote: ... The genomic DNA was sheared to approximately 8-14 kb long fragments and a library was generated with the SMRTbell Express Template Prep Kit 2.0 (PacBio) without size selection ...
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bioRxiv - Genomics 2021Quote: ... and a SMRTbell library was prepared using the SMRT bell Template Prep Kit 1.0-SPv3 (Pacbio, 100-991-900). DNA quantification was performed using a Qubit fluorometer (Thermo Fisher ...
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bioRxiv - Genomics 2021Quote: ... Long-read DNA libraries were constructed using the SMRTbell Express Template Prep Kit 2.0 (PacBio, Menlo Park, CA, USA) and sequenced on SMRT cells (1M v3 LR ...
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bioRxiv - Genomics 2021Quote: The amplicon library was created using the SMRTbell Express Template Prep Kit 2.0 (PacBio, Cat. No. 100-938-900) as per manufacturer’s instructions ...
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bioRxiv - Genomics 2021Quote: ... PacBio SMRTbell libraries (∼20kb) were constructed using the SMRTbell Express Template Prep Kit 2.0 (PacBio, Menlo Park, CA, USA), following the manufacturer’s protocol ...
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bioRxiv - Genomics 2023Quote: ... The library preparation procedure is described in the protocol “Preparing HiFi Libraries from Low DNA Input Using SMRTbell Express Template Prep Kit 2.0” from PacBio. The SMRTbell library sizes and profiles were evaluated using the Agilent DNA 12000 kit on the Bioanalyzer system ...
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bioRxiv - Plant Biology 2023Quote: Library preparation and PacBio sequencing was conducted by Eremid Genomic Services (Kannapolis, NC) using the SMRTbell prep kit 3.0 (PacBio), following the manufacturer’s instruction ...
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bioRxiv - Genomics 2023Quote: ... we sheared 10 µg of HMW DNA to an average fragment size of of 24 kb and prepared two independent non-barcoded HiFi SMRTbell libraries using SMRTbell Express Template Prep Kit 2.0 (PacBio). We pooled the two libraries and performed size-selection with a BluePippin (SageScience ...
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bioRxiv - Genomics 2023Quote: ... HMW DNA was used to generate PacBio HiFi libraries via the Template Prep Kit v1 (PacBio, 100-259-100) or SMRTbell Express Template Prep Kit v2 (PacBio ...
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bioRxiv - Genomics 2022Quote: ... The long-read library was then sequenced on the PacBio Sequel IIe system in CLR mode using the Sequel II Binding Kit 2.2 (PacBio). The short-read library with an insert size of 350 bp was sequenced on the NovaSeq6000 platform (Illumina ...
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bioRxiv - Genomics 2023Quote: ... libraries were constructed with an average insert size of 15kb using SMRTbell Express Template Prep Kit 2.0 (Pacific Biosciences, Menlo Park, USA) and sequenced by PacBio Sequel II ...
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bioRxiv - Evolutionary Biology 2023Quote: ... SMRTbell libraries (∼20kb) for PacBio Sequel were constructed using SMRTbell Template Prep Kit 1.0 (PacBio, Menlo Park, CA, USA) using the manufacturer’s recommended protocol ...
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bioRxiv - Plant Biology 2024Quote: ... The final library was prepared with the Sequel® II binding kit 3.2 (PacBio Ref. No. 102-194-100) and was loaded with the diffusion loading mode with the on-plate concentration set at 90 pM on the Pacific Biosciences SEQUEL IIe System ...
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bioRxiv - Genetics 2024Quote: ... Fragments smaller than 40 kb were depleted from these samples using the SRE XL kit (PacBio, SKU102-208-400) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2024Quote: ... and purification of polymerase-bound SMRTbell complexes were performed using the Revio polymerase kit (PacBio, Menlo Park, CA, USA). Loading concentration for sequencing was 225 pM ...
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bioRxiv - Genetics 2023Quote: ... DNA was then cleaned and sheared to an average size of 19 kb before generating four HiFi libraries using SMRTbell template express kit 2.0 (PacBio). Final libraries were size-selected in the size range of 7-50kb and sequenced across 18 SMRT cells (see SI for details).
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bioRxiv - Evolutionary Biology 2023Quote: ... We prepared a PacBio HiFi library with the SMRTbell Express Template Prep Kit 2.0 (PacBio, Menlo Park, CA, USA). The library was sequenced on a PacBio Sequel II instrument on an 8M SMRT cell in CCS mode with a 30-hour movie time ...
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bioRxiv - Genomics 2024Quote: ... The resulting cDNA was used as input for the MAS-Seq for 10x Single Cell 3’ kit by PacBio.
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bioRxiv - Genomics 2024Quote: ... a SMRT bell library was constructed using the SMRTbell® prep kit 3.0 (PacBio Ref. No. 102-141-700), following the manufacturer’s instructions ...
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bioRxiv - Genomics 2024Quote: A SMRTbell library was then constructed with the SMRTbell® prep kit 3.0 (PacBio Ref. No. 102-141-700), following the manufacturer’s protocol ...
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bioRxiv - Genomics 2021Quote: A long-read sequence library was prepared using the SMRTbell Express Template Prep Kit 1.0 (PacBio, Menlo Park, CA, USA). The size selection of the library was performed by BluePippin (Sage Science ...
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bioRxiv - Neuroscience 2021Quote: ... The SMRT sequencing was performed on the PacBio Sequel at the Genomics Core at KU Leuven (Belgium) using the Clontech SMARTer PCR cDNA Synthesis Kit (recommended by Pacbio). Multiple parallel PCR reactions were set after the first strand synthesis employing the PrimeSTAR GXL DNA Polymerase kit ...
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bioRxiv - Microbiology 2021Quote: ... to 15 kb and size-selected using the Blue Pippin (Sage Science, USA) and prepared for sequencing using SMRTbell Express Template Preparation Kit 1.0 (PacBio, USA) according to manufacturers’ instructions ...