Labshake search
Citations for PacBio :
251 - 300 of 305 citations for Cow T Cell Surface Glycoprotein CD3 Gamma Chain CD3G ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Evolutionary Biology 2023Quote: ... The PacBio SMRTbell library (∼20kb) for PacBio Sequel was constructed using SMRTbell Express Template Prep Kit 2.0 (PacBio, Menlo Park, CA, USA) using the manufacturer recommended protocol ...
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bioRxiv - Genomics 2024Quote: ... The final preparation of the library was performed using the Sequel® II binding kit 3.2 (PacBio Ref. No. 102-194-100). In brief ...
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bioRxiv - Evolutionary Biology 2024Quote: ... The PacBio SMRTbell library (∼20kb) for PacBio Sequel was constructed using SMRTbell Express Template Prep Kit 2.0 (PacBio, Menlo Park, CA, USA) using the manufacturer’s recommended protocol ...
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bioRxiv - Genomics 2024Quote: ... The purified linearized library was used to prepare a PacBio sequencing library using a SMRTbell prep kit 3.0 (PacBio #102-141-700), following the manufacturer’s recommended protocol (PacBio Protocol #102-166-600 REV02 ...
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bioRxiv - Genomics 2024Quote: The final library preparation for sequencing was performed with The Sequel® II binding kit 3.2 (PacBio Ref. No. 102-194-100). In brief ...
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bioRxiv - Genomics 2024Quote: ... The PacBio SMRTbell library (∼20kb) for PacBio Sequel was constructed using SMRTbell Express Template Prep Kit 2.0 (PacBio, Menlo Park, CA, USA) using the manufacturer-recommended protocol ...
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bioRxiv - Evolutionary Biology 2024Quote: ... The PacBio SMRTbell library (∼20kb) for PacBio Sequel was constructed using SMRTbell Express Template Prep Kit 2.0 (PacBio, Menlo Park, CA, USA) using the manufacturer recommended protocol ...
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bioRxiv - Genomics 2020Quote: ... in accordance with the manufacture’s protocol and sequenced with SMRT Cell v2.1 on a Sequel System (PacBio). The sequence reads were assembled using FALCON v.1.8.814 to generate primary contig sequences and to associate contigs representing alternative alleles ...
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bioRxiv - Microbiology 2020Quote: ... The genome of the strain QMA0248 was sequenced using 3 SMRT cells on the Pacific Biosciences (PacBio) RS II platform and P4C2 sequencing chemistry ...
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bioRxiv - Microbiology 2021Quote: ... the RK13 cells we used were confirmed to be of European rabbit (Oryctolagus cuniculus) origin by PacBio sequencing (ArrayExpress accession ...
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bioRxiv - Genetics 2019Quote: ... SMRTbell libraries were annealed and bound to P6 DNA polymerase for sequencing using the DNA/Polymerase Binding Kit P6 v2.0 (PacBio Cat#100-372-700). Bound SMRTbell libraries were loaded onto SMRT cells using standard MagBead protocols and the MagBead Buffer Kit v2.0 (PacBio Cat#100-642-800) ...
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bioRxiv - Microbiology 2021Quote: ... Sheared DNA was treated with exonuclease to remove single-stranded ends and DNA damage repair mix followed by end repair and ligation of barcoded blunt adapters using SMRTbell Template Prep Kit 2.0 (PacBio, Menlo Park, CA, USA). Libraries were purified with AMPure PB beads (Beckman Coulter ...
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bioRxiv - Genomics 2021Quote: The manufacturer recommended protocol was used to generate a PacBio SMRTbell library (~20kb) for PacBio Sequel using SMRTbell Express Template Prep Kit 2.0 (PacBio, Menlo Park, CA, USA). The library was bound to polymerase using the Sequel II Binding Kit 2.0 (PacBio ...
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bioRxiv - Genomics 2022Quote: ... The sheared DNA was used for HiFi SMRTbell library preparation with the SMRTbell Express Template Prep Kit 2.0 (PacBio, Menlo Park, CA, USA). The resultant library was separated on BluePippin (Sage Science ...
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bioRxiv - Genomics 2022Quote: ... were added to ligation products following an adapted version of the PacBio Microbial Multiplexing PacBio protocol (Part Number 101-696-100 Version 07 (July 2020)) using the PacBio SMRTbell Express Template Prep Kit 2.0 (PacBio, PN: 100-938-900) as follows ...
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bioRxiv - Genomics 2022Quote: ... PacBio HiFi reads were generated by first constructing a ~20kb library using SMRTbell Express Template Prep Kit 2.0 (PacBio, Menlo Park, CA, USA) using the manufacturer’s recommended protocol ...
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bioRxiv - Microbiology 2022Quote: ... The sheared and purified DNA fragments were used as templates to prepare the SMRTbell library through SMRTbell template prep kit 1.0 (PacBio, Menlo Park, CA, USA), according to the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2023Quote: ... The PacBio SMRTbell library (~20kbp mean insert length) for PacBio Sequel was constructed using SMRTbell Express Template Prep Kit 2.0 (PacBio, Menlo Park, CA, USA) using the manufacturer recommended protocol ...
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bioRxiv - Genomics 2023Quote: ... The PacBio SMRTbell library (∼20 kbp mean insert length) for PacBio Sequel was constructed using SMRTbell Express Template Prep Kit 2.0 (PacBio, Menlo Park, CA, USA) using the manufacturer’s recommended protocol ...
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bioRxiv - Genomics 2023Quote: ... Sheared DNA was treated with exonuclease to remove single stranded ends and DNA damage repair mix followed by end repair and ligation of barcoded blunt adapters using SMRTbell Template Prep Kit 2.0 (PacBio, Menlo Park, CA, USA). Libraries were purified with AMPure PB beads (Beckman Coulter ...
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bioRxiv - Genomics 2023Quote: ... The PacBio SMRTbell library (∼20kb) for PacBio Sequel was constructed using the SMRTbell Express Template Prep Kit 2.0 (PacBio, Menlo Park, CA, USA) following the manufacturer recommended protocol ...
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bioRxiv - Evolutionary Biology 2023Quote: ... we performed a DNA damage repair reaction followed by exonuclease treatment (ExoIII and ExoVII) using the SMRTbell DNA Damage Repair Kit (PacBio # 100-486-900) according manufacturer’s instruction ...
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bioRxiv - Evolutionary Biology 2023Quote: ... We then pooled all the barcoded libraries to be multiplexed and purified the pooled library using the AMPure PB Kit (PacBio # 100-265-900). We used a concentration of 0.6X of AMPure Beads and eluted the DNA in 37 µl of elution buffer ...
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bioRxiv - Genomics 2023Quote: ... The PacBio SMRTbell library (∼20 kb) for PacBio Sequel was constructed using SMRTbell Express Template Prep Kit 2.0 (PacBio, Menlo Park, CA, USA) using the manufacturer recommended protocol ...
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bioRxiv - Genomics 2024Quote: ... A SMRTbell library was then prepared by following the protocol of the SMRTbell® prep kit 3.0 (PacBio Ref. No. 102-141-700). Briefly ...
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bioRxiv - Evolutionary Biology 2023Quote: ... we used 80 ng of each library in the end-repair/ligation reaction using barcoded adaptors from the SMRTbell barcoded adapter complete prep kit -96 (PacBio # 100-514-900). We then pooled all the barcoded libraries to be multiplexed and purified the pooled library using the AMPure PB Kit (PacBio # 100-265-900) ...
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bioRxiv - Cancer Biology 2023Quote: ... All samples were depleted of short DNA fragments (less than 10kb long) with the short read eliminator (SRE) kit (Circulomics, SS-100-101-01; now PacBio 102-208-300) or the SRE XS kit (Circulomics SS-100-121-01 ...
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bioRxiv - Genomics 2023Quote: ... They constructed the PacBio SMRTbell library (∼20kb) for PacBio Sequel using SMRTbell Express Template Prep Kit V 2.0 (PacBio, Menlo Park, CA, USA) using the manufacturer recommended protocol ...
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bioRxiv - Genomics 2022Quote: ... The fractionated DNA libraries were sequenced on SMRT cells on the Sequel II and Sequel IIe system (PacBio). HiFi reads were constructed with the CCS pipeline (https://ccs.how).
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bioRxiv - Evolutionary Biology 2021Quote: ... DNA was extracted using the Qiagen kits Dneasy (for short-read sequencing; from approximately 5000-7000 rotifers) and GenomicTips 100 (for long read Pacbio sequencing; from >20000 rotifers), and RNA was extracted from freshly prepared biomass with Rneasy.
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bioRxiv - Genomics 2019Quote: ... The nine cDNAs were pooled in equimolar ratios and used to prepare a SMRTbell™ library using the PacBio Template Prep Kit (PacBio, Menlo Park, CA). The SMRTbell™ library was then sequenced across four Sequel v2 SMRT cells with polymerase 2.1 and chemistry 2.1 (P2.1C2.1).
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bioRxiv - Neuroscience 2020Quote: ... PacBio library preparation was performed with 500 ng of amplified cDNA using SMRTbell Express Template Prep Kit V2.0 (PacBio cat#PN: 100-938-900) to obtain Sequel II compatible library complex and was sequenced on a total 24 Sequel I SMRTcells with a run time of 10 hours and 20 Sequel II SMRTcells with a run time of 30 hours across samples and replicates.
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bioRxiv - Genomics 2020Quote: ... The three cDNAs were pooled in equimolar ratios and used to prepare a SMRTbell™ library using the PacBio Template Prep Kit (PacBio, Menlo Park, CA). The SMRTbell™ library was then sequenced on a Sequel v2 SMRT cell with polymerase 2.1 and chemistry 2.1 (P2.1C2.1 ...
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bioRxiv - Genomics 2023Quote: The HiFi SMRTbell library was constructed using the SMRTbell Express Template Prep Kit v2.0 (Pacific Biosciences -PacBio, Menlo Park, CA, Cat. #100-938-900) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2023Quote: ... Short read elimination was performed using the Circulomics Short Read Eliminator kit (formerly SS-100-101-01, now SKU 102-208-300; PacBio, Menlo Park, California, USA). The size selected DNA was quantified using a Qubit 1.0 Fluorometer (Thermo Fisher Scientific ...
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bioRxiv - Plant Biology 2023Quote: ... a fraction of short fragments was removed from 9 μg of total DNA using the Short-Read Eliminator Kit XL (PacBio, SKU 102-208-400), according to the manufacturer’s recommendations ...
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bioRxiv - Microbiology 2024Quote: Extraction of high molecular weight DNA from overnight cultures of CPs was accomplished using the Nanobind CBB kit (PacBio catalog no.102-301-900). Barcoded sequencing libraries were prepared using the SMRTbell prep kit 3.0 (PacBio catalog no.102-141-700 ...
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bioRxiv - Genomics 2024Quote: High molecular weight genomic DNA was extracted from heart tissue according to the protocol of (Sambrook and Russell) for the first library and from lung tissue using the PacBio Nanobind Tissue Kit (PacBio, Menlo Park, CA, USA) for the second library ...
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bioRxiv - Cancer Biology 2023Quote: Tumour genomic DNA was extracted from fresh-frozen tissue with the Nanobind tissue kit (Circulomics NB-900-701-01, now PacBio SKU 102-302-100) and Germline DNA from blood with the Nanobind CBB kit (Circulomics NB-900-001-01 ...
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bioRxiv - Plant Biology 2020Quote: ... A total of 24 Single-Molecule Real-Time (SMRT) cells (9 from PacBio RSII and 15 from PacBio Sequel) for Korso and 15 SMRT cells (all from PacBio Sequel ...
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bioRxiv - Plant Biology 2020Quote: ... A total of 24 Single-Molecule Real-Time (SMRT) cells (9 from PacBio RSII and 15 from PacBio Sequel) for Korso and 15 SMRT cells (all from PacBio Sequel ...
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bioRxiv - Genomics 2023Quote: ... Libraries were sequenced on the Sequel II platform with seven or eight SMRT Cells 8M (PacBio, 101-389-001) per sample using either Sequel II Sequencing Chemistry 1.0 (PacBio ...
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bioRxiv - Genomics 2023Quote: ... We sequenced the library on a single SMRT Cell (30 hours movie time) with the Sequel II system (PacBio) using the Binding Kit 2.0 ...
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bioRxiv - Genomics 2023Quote: The HiFi SMRTbell library was constructed using the SMRTbell Express Template Prep Kit v2.0 following the manufacturer’s protocols (Pacific Biosciences - PacBio, Menlo Park, CA; Cat. #100-938-900). HMW gDNA was sheared to a target DNA size distribution between 15-20 Kb and concentrated using 0.45X of AMPure PB beads (PacBio Cat ...
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bioRxiv - Microbiology 2020Quote: ... and sequenced at Washington State University core facility using one cell of the single molecular real time (SMRT) Pacific Biosciences (PacBio) RSII system.
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bioRxiv - Genomics 2022Quote: The absolute quantity of DNA-m6A in S2 cell nuclei treated with 20 U of Hia5 for 10 min was determined using Pacific Biosciences (PacBio) Circular consensus sequencing ...
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bioRxiv - Microbiology 2022Quote: ... DNA extraction, library preparation, sequencing (Pacific Biosciences Sequel, 4 samples on one SMRT cell) and assembly (‘Microbial Assembly’ function in PacBio SMRT® Link v8.0 for ACAM34_DSMZ and flye assembly tool v2.6 [65] for ACAM34_UNSW ...
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bioRxiv - Plant Biology 2022Quote: ... USA) to eliminate fragments <20 kb and sequenced with a Four SMRT Cell 8M on the Sequel II system (PacBio). The sequence reads were converted into HiFi reads with the ccs pipeline (PacBio ...
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bioRxiv - Genomics 2023Quote: Three HPG1 accessions (Exposito-Alonso et al. 2018) were sequenced with one SMRT Cell on the Sequel II platform (PacBio). Plant growth (Contreras-Garrido et al ...
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bioRxiv - Genomics 2023Quote: Sequencing data were obtained using two complementary long-read sequencing technologies for the assembly of hTERT RPE-1 cells: Pacific Biosciences (PacBio) High-Fidelity (HiFi ...