Labshake search
Citations for PacBio :
251 - 300 of 326 citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2022Quote: ... A linked-read sequencing library was prepared using the 10X Genomics Chromium Genomic Kit (from the same DNA extraction as used for PacBio sequencing ...
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bioRxiv - Molecular Biology 2023Quote: High molecular weight DNA was extracted from frozen pellets (1 million cells) using the Nanobind HMW DNA Extraction kit (PacBio) following the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2023Quote: ... Libraries were prepared and multiplexed with the PacBio SMRTbell® Express Template Prep Kit 2.0 (PacBio, Menlo Park, CA, USA) according to the published protocol (19) ...
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bioRxiv - Genomics 2023Quote: ... DNA was pooled and sequenced at the Australian Genome Research Facility (Brisbane, Queensland, Australia) using a SMRTbell® prep kit 3.0 (PacBio), and circular consensus sequencing (CCS ...
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bioRxiv - Genomics 2023Quote: ... Extractions were performed using the Nanobind Tissue Big DNA kit following the manufacturer’s instructions (Pacific BioSciences - PacBio, Menlo Park, CA). For song sparrow ...
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bioRxiv - Genomics 2024Quote: ... DNA was then isolated from the washed blood pellet using the Circulomics Nanobind CBB kit and protocol according to the manufacturer’s recommendations (Circulomics, now PacBio company). High molecular weight DNA was eluted from the Nanodisk with 150µl Tris-cl buffer ...
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bioRxiv - Evolutionary Biology 2024Quote: ... A PacBio SMRTbell library was prepared using the SMRTbell Template Prep Kit 2.0 and Sequel II Binding 2.2 (PacBio, CA, USA), and loaded onto the sequencer by diffusion ...
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bioRxiv - Molecular Biology 2024Quote: High molecule weight DNA was extracted from patient’s HSPC with the Nanobind CBB kit (PacBio, catalog no. 102-301-900) according to manufacturer’s instructions ...
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bioRxiv - Plant Biology 2024Quote: ... The PacBio SMRTbell library (15-20kb) was constructed using SMRTbell Express Template Prep Kit 3.0 (PacBio, Menlo Park, CA, USA) using the manufacturer recommended protocol ...
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bioRxiv - Cancer Biology 2024Quote: ... All samples were depleted of short DNA fragments (less than 10kb long) with the SRE kit (Circulomics, SS-100-101-01, now PacBio) or the SRE XS kit (Circulomics SS-100-121-01 ...
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bioRxiv - Evolutionary Biology 2024Quote: ... The resulting nuclei/cellular compartment mix was used as input for the Nanobind plant nuclei big DNA kit from PacBio. Steps were followed as listed in the kit protocol except for large cell inputs ...
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bioRxiv - Genomics 2021Quote: ... The size-selected DNA was prepared into a PacBio library using the SMRTbell® Express Template Preparation Kit 2.0 (PacBio, USA), then sequenced across two PacBio Sequel II 8M SMRT cells (PN:101-389-001) ...
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bioRxiv - Genomics 2021Quote: ... Sequencing libraries for each sample were created using the SMRTbell Express Template Prep Kit 2.0 (PacBio, Cat. No. 100-938-900) per manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... Samples with many short DNA fragments were further purified using either the Short Read Eliminator kit (PacBio, San Diego, CA, USA), or 0.4 - 0.5 x volume KAPA PURE SPRI beads (Roche ...
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bioRxiv - Immunology 2023Quote: ... PacBio SMRTbell amplicon libraries for PacBio Sequel were constructed using SMRTbell Express Template Prep Kit 2.0 (PacBio, Menlo Park, CA, USA) using the manufacturer recommended protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... and Germline DNA from blood with the Nanobind CBB kit (Circulomics NB-900-001-01, now PacBio SKU 102-301-900). After extraction ...
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bioRxiv - Genomics 2024Quote: ... using settings 28/31 and used to generate PacBio HiFi libraries via the SMRTbell Prep Kit 3.0 (PacBio, 102-182-700). Size selection was performed with Pippin HT using a high-pass cutoff of 17 kbp (Sage Science ...
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bioRxiv - Genetics 2023Quote: HiFi SMRTbell libraries were constructed according to the manufacturer’s manual by using SMRTbell Express Template Prep Kit 2.0 (PacBio, 100-938-900). For all samples ...
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bioRxiv - Genomics 2023Quote: ... The PacBio SMRTbell library (∼20kb) for PacBio Sequel was constructed using SMRTbell Template Prep Kit 1.0 (PacBio, Menlo Park, CA, USA) using the manufacturer recommended protocol ...
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bioRxiv - Evolutionary Biology 2023Quote: ... elegans female of each genotype (Ao, Io, oo), using the Nanobind® Tissue Big Extraction Kit (Cat. No. NB-900-701-01, Circulomics Inc. (PacBio), MD ...
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bioRxiv - Genomics 2024Quote: ... A SMRTbell library was constructed for both samples using the SMRTbell® prep kit 3.0 (PacBio Cat. No. 102-141-700), following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... The purified ligation product was split into two reactions and enriched using 10-18 cycles of PCR using barcoded amplification oligos (IDT) and SMRTbell® gDNA Sample Amplification Kit (PacBio). Up to sixteen libraries were pooled in equimolar concentrations and the pooled libraries were size-selected using the 0.75% agarose gel cassettes with Marker S1 and High Pass protocol on the BluePippin (Sage Science) ...
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bioRxiv - Evolutionary Biology 2024Quote: ... was extracted from muscle tissue that was preserved in ethanol using the Circulomics Nanobind Tissue Big DNA kit (Circulomics Inc., PacBio, USA) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2024Quote: ... Fragments less than 5 kb were selectively removed using the AMPure PB beads size selection kit (Pacbio, Menlo Park, CA, USA). The SMRTbell® prep kit 3.0 from Pacbio was used to prepare the sequencing libraries ...
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bioRxiv - Genomics 2024Quote: ... The PacBio SMRTbell library for PacBio Sequel was constructed using SMRTbell Express Template Prep Kit 2.0 (PacBio, Menlo Park, CA, USA) using the manufacturer-recommended protocol ...
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bioRxiv - Genomics 2020Quote: ... The polymerase-bound library was sequenced on 8 SMRT Cells with a 10 h movie time using the Sequel Sequencing Kit 3.0 (PacBio, 101-597-900) and a Sequel SMRT Cell 1M v3 (PacBio ...
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bioRxiv - Microbiology 2020Quote: ... eluted in 37 μl EB (10 mM TrisHCl pH 8.0) and processed using SMRTBellTM Template Prep kit 1.0 (PacBio Part# 100-259-100) to SMRTbell libraries ...
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bioRxiv - Genomics 2022Quote: ... The PacBio SMRTbell library (~20kb) for PacBio Sequel was constructed using SMRTbell Express Template Prep Kit 2.0 (PacBio, Menlo Park, CA, USA) using the manufacturer recommended protocol ...
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bioRxiv - Genomics 2024Quote: The final library preparation for sequencing was performed with The Sequel® II binding kit 3.2 (PacBio Ref. No. 102-194-100). In brief ...
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bioRxiv - Genomics 2024Quote: ... The PacBio SMRTbell library (∼20kb) for PacBio Sequel was constructed using SMRTbell Express Template Prep Kit 2.0 (PacBio, Menlo Park, CA, USA) using the manufacturer-recommended protocol ...
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bioRxiv - Evolutionary Biology 2024Quote: ... The PacBio SMRTbell library (∼20kb) for PacBio Sequel was constructed using SMRTbell Express Template Prep Kit 2.0 (PacBio, Menlo Park, CA, USA) using the manufacturer recommended protocol ...
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bioRxiv - Genomics 2024Quote: ... The final preparation of the library was performed using the Sequel® II binding kit 3.2 (PacBio Ref. No. 102-194-100). In brief ...
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bioRxiv - Genomics 2023Quote: HMW DNA from 1.5 x 106 cells was used to generate PacBio HiFi libraries with the SMRTbell express template prep kit 2.0 (PacBio 100-938-900). Size selection was performed with Megaruptor 2 (Diagenode ...
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bioRxiv - Evolutionary Biology 2024Quote: ... The PacBio SMRTbell library (∼20kb) for PacBio Sequel was constructed using SMRTbell Express Template Prep Kit 2.0 (PacBio, Menlo Park, CA, USA) using the manufacturer’s recommended protocol ...
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bioRxiv - Genomics 2024Quote: ... The purified linearized library was used to prepare a PacBio sequencing library using a SMRTbell prep kit 3.0 (PacBio #102-141-700), following the manufacturer’s recommended protocol (PacBio Protocol #102-166-600 REV02 ...
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bioRxiv - Genomics 2023Quote: The PacBio SMRTbell library (∼20kb) for PacBio Sequel was constructed using SMRTbell Express Template Prep Kit 2.0 (PacBio, Menlo Park, CA, USA) using the manufacturer recommended protocol ...
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bioRxiv - Genetics 2023Quote: ... UW then prepared Iso-seq libraries from this cDNA using a Iso-Seq Express SMRTbell library prep kit (PacBio #100-938-900). The resulting iso-seq libraries were then sequenced by UW on a PacBio Sequel II system using an 8M SMRT cell (v2.1 chemistry) ...
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bioRxiv - Genomics 2023Quote: ... a SMRTbell DNA library was constructed according to the Pacbio HiFi SMRTbell protocol using SMRTbell Express Template Prep Kit 3.0 (Pacbio, 102-182-700). After BluePippin size selection (Sage Science ...
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bioRxiv - Genomics 2023Quote: ... The DNA was then selected for high molecular weight DNA using the PacBio Short Read Eliminator kit (PacBio, Menlo Park, CA, USA). Concentration was assessed using the dsDNA BR assay on a Qubit fluorometer (Thermo Fisher Scientific ...
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bioRxiv - Evolutionary Biology 2023Quote: ... The PacBio SMRTbell library (∼20kb) for PacBio Sequel was constructed using SMRTbell Express Template Prep Kit 2.0 (PacBio, Menlo Park, CA, USA) using the manufacturer recommended protocol ...
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bioRxiv - Systems Biology 2024Quote: ... A HiFi sequencing library for de novo genome assembly was prepared using the SMRTbell library prep kit (PacBio, Menlo Park, CA, USA), according to the manufacturer’s protocol ...
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bioRxiv - Genomics 2024Quote: ... and constructed PacBio SMRTbell library (∼20 kb) for PacBio Sequel using SMRTbell Express Template Prep Kit 2.0 (PacBio, Menlo Park, CA, USA) following the manufacturer recommended protocol ...
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bioRxiv - Plant Biology 2024Quote: ... Sequencing primer v4 was annealed to the Iso-Seq library pool and complexed to the sequencing polymerase with the Sequel II binding kit v2.1 (PacBio, 101-843-000). Calculations for primer to template and polymerase to template binding ratios were kept at default values for the library type ...
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bioRxiv - Genomics 2024Quote: ... The final library preparation for sequencing was conducted using The Sequel® II binding kit 3.2 (PacBio Ref. No. 102-194-100) The library was loaded on-plate in diffusion mode at concentration of 90 pM followed by sequencing on the Pacific Biosciences SEQUEL IIe System at The Chinese University of Hong Kong under the following conditions ...
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bioRxiv - Bioengineering 2024Quote: High molecular weight genomic DNA was isolated from NHP liver tissues using Nanobind Tissue kit from PacBio (Cat. No. 102-302-100) according to manufacturer’s protocol ...
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bioRxiv - Microbiology 2021Quote: ... Sheared DNA was treated with exonuclease to remove single-stranded ends and DNA damage repair mix followed by end repair and ligation of barcoded blunt adapters using SMRTbell Template Prep Kit 2.0 (PacBio, Menlo Park, CA, USA). Libraries were purified with AMPure PB beads (Beckman Coulter ...
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bioRxiv - Genomics 2020Quote: A long-read sequence library was constructed using an SMRTbell Express Template Prep Kit 2.0 and sequenced on SMRT cells (1M v3 LR) in a PacBio Sequel system (PacBio, Menlo Park, CA, USA). The obtained reads (≥15 kb ...
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bioRxiv - Genomics 2021Quote: The manufacturer recommended protocol was used to generate a PacBio SMRTbell library (~20kb) for PacBio Sequel using SMRTbell Express Template Prep Kit 2.0 (PacBio, Menlo Park, CA, USA). The library was bound to polymerase using the Sequel II Binding Kit 2.0 (PacBio ...
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bioRxiv - Genomics 2022Quote: ... The sheared DNA was used for HiFi SMRTbell library preparation with the SMRTbell Express Template Prep Kit 2.0 (PacBio, Menlo Park, CA, USA). The resultant library was separated on BluePippin (Sage Science ...
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bioRxiv - Genomics 2022Quote: ... were added to ligation products following an adapted version of the PacBio Microbial Multiplexing PacBio protocol (Part Number 101-696-100 Version 07 (July 2020)) using the PacBio SMRTbell Express Template Prep Kit 2.0 (PacBio, PN: 100-938-900) as follows ...