Labshake search
Citations for PacBio :
201 - 250 of 281 citations for Mouse Lymphoid Enhancer Binding Factor 1 LEF1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Genetics 2023Quote: ... DNA was then cleaned and sheared to an average size of 19 kb before generating four HiFi libraries using SMRTbell template express kit 2.0 (PacBio). Final libraries were size-selected in the size range of 7-50kb and sequenced across 18 SMRT cells (see SI for details).
-
bioRxiv - Evolutionary Biology 2023Quote: ... We prepared a PacBio HiFi library with the SMRTbell Express Template Prep Kit 2.0 (PacBio, Menlo Park, CA, USA). The library was sequenced on a PacBio Sequel II instrument on an 8M SMRT cell in CCS mode with a 30-hour movie time ...
-
bioRxiv - Genomics 2024Quote: ... The resulting cDNA was used as input for the MAS-Seq for 10x Single Cell 3’ kit by PacBio.
-
bioRxiv - Genomics 2024Quote: ... a SMRT bell library was constructed using the SMRTbell® prep kit 3.0 (PacBio Ref. No. 102-141-700), following the manufacturer’s instructions ...
-
bioRxiv - Genomics 2024Quote: A SMRTbell library was then constructed with the SMRTbell® prep kit 3.0 (PacBio Ref. No. 102-141-700), following the manufacturer’s protocol ...
-
bioRxiv - Microbiology 2020Quote: ... aeruginosa isolates were extracted and sequenced by Illumina HiSeq X platform and genome of Isolate 1(Iso1) was sequenced by PacBio Sequel II platform ...
-
bioRxiv - Genomics 2021Quote: A long-read sequence library was prepared using the SMRTbell Express Template Prep Kit 1.0 (PacBio, Menlo Park, CA, USA). The size selection of the library was performed by BluePippin (Sage Science ...
-
bioRxiv - Neuroscience 2021Quote: ... The SMRT sequencing was performed on the PacBio Sequel at the Genomics Core at KU Leuven (Belgium) using the Clontech SMARTer PCR cDNA Synthesis Kit (recommended by Pacbio). Multiple parallel PCR reactions were set after the first strand synthesis employing the PrimeSTAR GXL DNA Polymerase kit ...
-
bioRxiv - Microbiology 2021Quote: ... to 15 kb and size-selected using the Blue Pippin (Sage Science, USA) and prepared for sequencing using SMRTbell Express Template Preparation Kit 1.0 (PacBio, USA) according to manufacturers’ instructions ...
-
bioRxiv - Genomics 2022Quote: ... Sequencing libraries were prepared for each species using the SMRTbell Express Template Prep Kit 2.0 (PacBio, Menlo Park, CA, USA) and following the ultra-low protocol ...
-
bioRxiv - Genetics 2019Quote: 100-150ng of total RNA per reaction was reverse transcribed using the Clontech SMARTer cDNA synthesis kit and 12 sample specific barcoded oligo dT (with PacBio 16mer barcode sequences ...
-
bioRxiv - Genomics 2020Quote: ... The two fractions were recombined at equimolar quantities and library preparation performed using SMRTbell Template Prep Kit v1.0 (PacBio, USA). Sequencing was performed on the PacBio Sequel 1M SMRT cell ...
-
bioRxiv - Plant Biology 2022Quote: ... and a HiFi SMRTbell library was constructed with the SMRTbell Express Template Prep Kit 2.0 (PacBio, Menlo Park, CA, USA). The library DNA was fractionated using a BluePippin (Sage Science ...
-
bioRxiv - Genomics 2022Quote: ... A linked-read sequencing library was prepared using the 10X Genomics Chromium Genomic Kit (from the same DNA extraction as used for PacBio sequencing ...
-
bioRxiv - Plant Biology 2023Quote: ... Libraries were prepared and multiplexed with the PacBio SMRTbell® Express Template Prep Kit 2.0 (PacBio, Menlo Park, CA, USA) according to the published protocol (19) ...
-
bioRxiv - Genomics 2023Quote: ... DNA was pooled and sequenced at the Australian Genome Research Facility (Brisbane, Queensland, Australia) using a SMRTbell® prep kit 3.0 (PacBio), and circular consensus sequencing (CCS ...
-
bioRxiv - Genomics 2023Quote: ... Extractions were performed using the Nanobind Tissue Big DNA kit following the manufacturer’s instructions (Pacific BioSciences - PacBio, Menlo Park, CA). For song sparrow ...
-
bioRxiv - Molecular Biology 2024Quote: ... a long-read sequence library was prepared using the SMRTbell Express Template Prep Kit 2.0 (PacBio, Menlo Park, CA, USA) and sequenced using a PacBio Sequel II system (PacBio ...
-
bioRxiv - Genomics 2024Quote: ... DNA samples for Oxford Nanopore or PacBio long read sequencing were enriched for HMW DNA (> 25 kb) employing the Short Read Eliminator Kit (Circulomics/PacBio). Quality control was performed by determining A260/A280 and A260/A230 ratios on NanoDrop ...
-
bioRxiv - Evolutionary Biology 2024Quote: ... High molecular weight DNA was extracted from blood cells embedded in agarose blocks using the Nanobind CBB kit (Circulomics/PacBio). Construction of PacBio SMRT-bell sequencing libraries and long read sequencing on PacBio Sequel II were conducted at the Roy J ...
-
bioRxiv - Genetics 2020Quote: ... demultiplexed circular consensus (CCS) reads were generated with the RS_ReadsOfInsert.1 protocol from PacBio’s SMRT portal (v2.3.0 ...
-
bioRxiv - Genomics 2021Quote: ... The size-selected DNA was prepared into a PacBio library using the SMRTbell® Express Template Preparation Kit 2.0 (PacBio, USA), then sequenced across two PacBio Sequel II 8M SMRT cells (PN:101-389-001) ...
-
bioRxiv - Genomics 2021Quote: ... Sequencing libraries for each sample were created using the SMRTbell Express Template Prep Kit 2.0 (PacBio, Cat. No. 100-938-900) per manufacturer’s instructions ...
-
bioRxiv - Genomics 2023Quote: ... The PacBio SMRTbell library (∼20kb) for PacBio Sequel was constructed using SMRTbell Template Prep Kit 1.0 (PacBio, Menlo Park, CA, USA) using the manufacturer recommended protocol ...
-
bioRxiv - Genetics 2023Quote: HiFi SMRTbell libraries were constructed according to the manufacturer’s manual by using SMRTbell Express Template Prep Kit 2.0 (PacBio, 100-938-900). For all samples ...
-
bioRxiv - Plant Biology 2024Quote: ... the DNA was then size-selected using the Circulomics SRE kit v2.0 (SS-100-101-01, PacBio, Menlo Park, CA, USA). A concentration of 150 ng/µL of DNA was initially used ...
-
bioRxiv - Evolutionary Biology 2023Quote: ... elegans female of each genotype (Ao, Io, oo), using the Nanobind® Tissue Big Extraction Kit (Cat. No. NB-900-701-01, Circulomics Inc. (PacBio), MD ...
-
bioRxiv - Microbiology 2024Quote: ... Samples with many short DNA fragments were further purified using either the Short Read Eliminator kit (PacBio, San Diego, CA, USA), or 0.4 - 0.5 x volume KAPA PURE SPRI beads (Roche ...
-
bioRxiv - Immunology 2023Quote: ... PacBio SMRTbell amplicon libraries for PacBio Sequel were constructed using SMRTbell Express Template Prep Kit 2.0 (PacBio, Menlo Park, CA, USA) using the manufacturer recommended protocol ...
-
bioRxiv - Genomics 2024Quote: ... using settings 28/31 and used to generate PacBio HiFi libraries via the SMRTbell Prep Kit 3.0 (PacBio, 102-182-700). Size selection was performed with Pippin HT using a high-pass cutoff of 17 kbp (Sage Science ...
-
bioRxiv - Cancer Biology 2023Quote: ... and Germline DNA from blood with the Nanobind CBB kit (Circulomics NB-900-001-01, now PacBio SKU 102-301-900). After extraction ...
-
bioRxiv - Microbiology 2019Quote: ... The long-read libraries were prepared with a SMRTbell library using a SMRTbell template prep kit 1.0 (PacBio, Menlo Park, CA, USA) with barcoding adapters according to the manufacturer’s instructions ...
-
bioRxiv - Genomics 2020Quote: ... The polymerase-bound library was sequenced on 8 SMRT Cells with a 10 h movie time using the Sequel Sequencing Kit 3.0 (PacBio, 101-597-900) and a Sequel SMRT Cell 1M v3 (PacBio ...
-
bioRxiv - Microbiology 2020Quote: ... eluted in 37 μl EB (10 mM TrisHCl pH 8.0) and processed using SMRTBellTM Template Prep kit 1.0 (PacBio Part# 100-259-100) to SMRTbell libraries ...
-
bioRxiv - Genomics 2022Quote: ... The PacBio SMRTbell library (~20kb) for PacBio Sequel was constructed using SMRTbell Express Template Prep Kit 2.0 (PacBio, Menlo Park, CA, USA) using the manufacturer recommended protocol ...
-
bioRxiv - Genomics 2023Quote: ... The DNA was then selected for high molecular weight DNA using the PacBio Short Read Eliminator kit (PacBio, Menlo Park, CA, USA). Concentration was assessed using the dsDNA BR assay on a Qubit fluorometer (Thermo Fisher Scientific ...
-
bioRxiv - Genomics 2023Quote: ... a SMRTbell DNA library was constructed according to the Pacbio HiFi SMRTbell protocol using SMRTbell Express Template Prep Kit 3.0 (Pacbio, 102-182-700). After BluePippin size selection (Sage Science ...
-
bioRxiv - Genetics 2023Quote: ... UW then prepared Iso-seq libraries from this cDNA using a Iso-Seq Express SMRTbell library prep kit (PacBio #100-938-900). The resulting iso-seq libraries were then sequenced by UW on a PacBio Sequel II system using an 8M SMRT cell (v2.1 chemistry) ...
-
bioRxiv - Genomics 2023Quote: The PacBio SMRTbell library (∼20kb) for PacBio Sequel was constructed using SMRTbell Express Template Prep Kit 2.0 (PacBio, Menlo Park, CA, USA) using the manufacturer recommended protocol ...
-
bioRxiv - Evolutionary Biology 2023Quote: ... The PacBio SMRTbell library (∼20kb) for PacBio Sequel was constructed using SMRTbell Express Template Prep Kit 2.0 (PacBio, Menlo Park, CA, USA) using the manufacturer recommended protocol ...
-
bioRxiv - Genomics 2024Quote: ... The PacBio SMRTbell library (∼20kb) for PacBio Sequel was constructed using SMRTbell Express Template Prep Kit 2.0 (PacBio, Menlo Park, CA, USA) using the manufacturer-recommended protocol ...
-
bioRxiv - Evolutionary Biology 2024Quote: ... The PacBio SMRTbell library (∼20kb) for PacBio Sequel was constructed using SMRTbell Express Template Prep Kit 2.0 (PacBio, Menlo Park, CA, USA) using the manufacturer recommended protocol ...
-
bioRxiv - Genomics 2023Quote: HMW DNA from 1.5 x 106 cells was used to generate PacBio HiFi libraries with the SMRTbell express template prep kit 2.0 (PacBio 100-938-900). Size selection was performed with Megaruptor 2 (Diagenode ...
-
bioRxiv - Evolutionary Biology 2024Quote: ... The PacBio SMRTbell library (∼20kb) for PacBio Sequel was constructed using SMRTbell Express Template Prep Kit 2.0 (PacBio, Menlo Park, CA, USA) using the manufacturer’s recommended protocol ...
-
bioRxiv - Genomics 2024Quote: ... The purified linearized library was used to prepare a PacBio sequencing library using a SMRTbell prep kit 3.0 (PacBio #102-141-700), following the manufacturer’s recommended protocol (PacBio Protocol #102-166-600 REV02 ...
-
bioRxiv - Genomics 2020Quote: We generated circular consensus reads by ccs tool with “--noPolish --minPasses 1” options from PacBio SMRT link tools v.5.1.0 (https://www.pacb.com/support/software-downloads/ ...
-
bioRxiv - Genetics 2019Quote: ... Bound SMRTbell libraries were loaded onto SMRT cells using standard MagBead protocols and the MagBead Buffer Kit v2.0 (PacBio Cat#100-642-800). The standard MagBead sequencing protocol was followed with the DNA Sequencing Kit 4.0 v2 (PacBio Cat#100-612-400 ...
-
bioRxiv - Microbiology 2021Quote: ... Sheared DNA was treated with exonuclease to remove single-stranded ends and DNA damage repair mix followed by end repair and ligation of barcoded blunt adapters using SMRTbell Template Prep Kit 2.0 (PacBio, Menlo Park, CA, USA). Libraries were purified with AMPure PB beads (Beckman Coulter ...
-
bioRxiv - Genomics 2020Quote: A long-read sequence library was constructed using an SMRTbell Express Template Prep Kit 2.0 and sequenced on SMRT cells (1M v3 LR) in a PacBio Sequel system (PacBio, Menlo Park, CA, USA). The obtained reads (≥15 kb ...
-
bioRxiv - Genomics 2021Quote: The manufacturer recommended protocol was used to generate a PacBio SMRTbell library (~20kb) for PacBio Sequel using SMRTbell Express Template Prep Kit 2.0 (PacBio, Menlo Park, CA, USA). The library was bound to polymerase using the Sequel II Binding Kit 2.0 (PacBio ...