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Citations for Promega :
51 - 100 of 2354 citations for ssc mir 369 RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... RT-PCR was performed with GoTaq G2 Green Master Mix (Promega) and gene specific primers ...
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bioRxiv - Molecular Biology 2022Quote: ... RT-PCR to validate splicing changes was performed using GoTaq (Promega) and quantified with Bioanalyzer RNA 6000 Nano assay (Agilent).
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bioRxiv - Neuroscience 2021Quote: ... RT-PCR analyses were performed using GoTaq DNA polymerase (#M7841, Promega) in a reaction volume of 25 μl comprising 1 μl of cDNA and primer pairs specific for the 5’-end (exon 1-3 ...
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bioRxiv - Genetics 2021Quote: ... RT-PCR was carried out using GoTaq Green Master Mix (Promega), following recommended protocol ...
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bioRxiv - Cell Biology 2023Quote: ... RT-PCR was performed using GoTaq Green Master Mix (Promega, M7123). 2 µl of diluted cDNA was added to 12.5 µl GoTaq Green Master Mix and 0.4 µM of forward and reverse primers and nuclease-free water in a 25 µl reaction ...
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bioRxiv - Microbiology 2024Quote: Real-time RT-qPCR assays (GoTaq 1-step qRT-PCR, Promega) were performed using standard fast cycling parameters (i.e ...
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bioRxiv - Synthetic Biology 2020Quote: ... Primers and remnants of the PCR reaction were removed using the Wizard PCR cleanup kit (Promega, USA). The concentration of the purified DNA was determined using an ND-2000 NanoDrop spectrophotometer ...
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bioRxiv - Biochemistry 2022Quote: ... The 400 base pair region surrounding the sgRNA target was then amplified by PCR (forward primer: 5’-CCCAGAGAGGAGGCTGTAGA-3’; reverse primer: 5’-AAAGGCCTCCCAGGGGTTAT-3’) with GoTaq DNA Polymerase (Promega). The resulting PCR product was cloned into the pCR 4-TOPO vector using the TOPO TA Cloning Kit for Sequencing (Invitrogen) ...
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bioRxiv - Plant Biology 2023Quote: The cDNA of BdNRT2A was first amplified by PCR using specific forward primer 70F2 and reverse primer 70R2 before being cloned into the pGEMT-easy vector (Promega). Clones with correct sequence were used to produced appropriate PCR product for Gateway cloning ...
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bioRxiv - Microbiology 2021Quote: ... spxB gene region containing the ORF (amplified from R6 genome using primer set 1-2) was cloned into pGEM-T Easy vector (Promega). spxB ORF was disrupted by cloning a kanamycin resistance cassette (amplified from pIBD38 ...
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bioRxiv - Molecular Biology 2021Quote: ... Quantitative RT-PCRs were performed with the GoTaq qPCR Master Mix (Promega), 2% of cDNA per reaction ...
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bioRxiv - Cell Biology 2019Quote: ... and analyzed by analytical RT-PCR using the GoTaq Green kit (Promega) with primers 1171 and 1174 (see Key Resources Table) ...
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bioRxiv - Plant Biology 2019Quote: ... RT-PCR was performed using GoTaq® DNA polymerase (Promega, https://www.promega.com) and corresponding primers for 28 cycles ...
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bioRxiv - Microbiology 2022Quote: Semi-quantitative RT-PCR was carried out using GoTaq DNA polymerase (Promega) with 16S rRNA primers (16S F and 16S R ...
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bioRxiv - Molecular Biology 2023Quote: RT-PCR was performed using the GoTaq G2 DNA Polymerase (M7845, Promega) with 3 μl of reverse transcriptase product and the following primers ...
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bioRxiv - Plant Biology 2023Quote: ... and RT-PCR was performed using Go-Taq polymerase (Promega, WI, USA).
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bioRxiv - Molecular Biology 2024Quote: ... RT-qPCR was performed using SYBR Green PCR Master Mix (A6002, Promega) with the gene-specific primers (Table S9) ...
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bioRxiv - Microbiology 2021Quote: ... Positive constructs were identified by colony PCR using the segment-specific PCR primers and Taq green mastermix (Promega) with the following cycle profile ...
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bioRxiv - Biophysics 2019Quote: ... The N-terminal Halo-tagged vector segment was cloned by using the primer sets: 5’-GAGTAACTAGCATAACCCCTTGGC-3’ and 5’-CACTAGCCATGTTATCGCTCTGAAAGTACAGATC-3’ with the pHTN HaloTag® CMV-neo Vector (Promega) as template ...
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bioRxiv - Neuroscience 2021Quote: ... Reverse transcription was performed on 1 μg of RNA with random hexamer primers using MMLV-RT enzyme (Promega) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2020Quote: The wild-type 3’UTR region of SALL4 mRNA or a mutant without the miR-205 binding site (Figure 4E) was amplified using PCR and cloned into the pGL3 vector (Promega, Madison, USA). HEK 293T cells were seeded into 24-well plates ...
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bioRxiv - Immunology 2019Quote: ... Primer-specific amplicons were produced by PCR using Taq Polymerase (Promega, Madison, WI), followed by a purification step using the Mini-Elute PCR purification kit (Qiagen ...
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bioRxiv - Genetics 2020Quote: ... Founder mice were analyzed by PCR using primers described in Table S1 and PCR products were subcloned in pGEM.T Easy Vector for sequencing (Promega). F1 and F2 mice were confirmed by PCR and by PCR products sequencing (primers available in Table S1) ...
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bioRxiv - Plant Biology 2024Quote: ... PCR reactions (25 µL) were set up with GoTaq Green Master Mix (Promega, Madison, WI, USA), 200 nM of forward and reverse primers (Table S40) ...
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bioRxiv - Cancer Biology 2021Quote: ... and real-time PCR using GoTaq 2-Step RT-qPCR kit (Promega, A6110). All measurements were normalized against Actin as the internal control using the 2-ΔΔCt method (Figure 1—source data 3 and Figure 3—source data 2) ...
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bioRxiv - Cell Biology 2019Quote: ... RT-PCR reactions were conducted using the GoTaq qPCR Master Mix (A6001, Promega) and a MasterCycler Ep-Realplex thermal cycler (Eppendorf).
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bioRxiv - Neuroscience 2020Quote: ... RT-PCR was performed using GoTaq® qPCR Master Mix (Promega, Shanghai, China) and specific primers (Sangon Biotech ...
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bioRxiv - Microbiology 2019Quote: Reverse transcriptase (RT)-PCR was carried out using ImProm-II reverse transcriptase (Promega) following the manufacturer’s protocol with 1 µg of RNA ...
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bioRxiv - Cell Biology 2022Quote: ... RT-PCR was then performed using the SYBR Green qPCR Master Mix (Promega). Primer sequences are available upon request ...
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bioRxiv - Microbiology 2023Quote: ... we confirmed that each primer set amplified single bands in a ligation- dependent manner by GoTaq Hot Start Green Master Mix (Promega, Madison, WI) (Figure S6) ...
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bioRxiv - Molecular Biology 2023Quote: ... 250 ng of DNAsed RNA were retro-transcribed using random hexamer primers with the M-MLV-RT enzyme (Promega). cDNA were amplified with the GoTaq qPCR Master Mix (Promega ...
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bioRxiv - Genetics 2023Quote: ... RNA (1000 ng input) was subsequently reverse-transcribed to cDNA using random primers and the GoScript RT kit (Promega). RT-qPCR was performed using FIREPoly qPCR Master Mix (Solis BioDyne ...
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bioRxiv - Neuroscience 2024Quote: ... BMSCs were transfected with miR-335-3p mimic, miR-335-3p inhibitor and NC that synthesized by RiboBio (Guangzhou, China) via Fugene HD (Promega, Madison, WI, USA; E2311) when cells confluency reached 50%.
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bioRxiv - Microbiology 2021Quote: To clone the 3xant-miR-275 into psiCheck-2 (Promega), two complementary single-stranded oligos that encode 3xant-miR-275 and XhoI and NotI restriction endonuclease cut sites were synthesized at Yale Keck Oligo Synthesis Resource (Table 1) ...
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bioRxiv - Cancer Biology 2023Quote: ... The isolated RNAs (20 ng) were reverse-transcribed and quantitative PCR (qPCR) were perform using GoTaq Probe 1-Step RT-PCR system (Promega) in a QuantStudio 7 Flex Real-Time PCR System (Applied Biosystems) ...
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bioRxiv - Neuroscience 2020Quote: ... Gene expression analysis was done by RT-PCR (GoTaq qPCR Master Mix, Promega, USA) using gene specific primers (Suppl ...
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bioRxiv - Neuroscience 2020Quote: ... Quantitative RT-PCR was performed in triplicates with the GoTaq qPCR Master Mix (Promega) on a LightCycler 480 II PCR system (Roche ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... RT-PCR was carried out using SYBR Green master mix (Sybr Green-A6001 Promega) for detection in Light cycler LC 480 (Roche) ...
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bioRxiv - Microbiology 2020Quote: ... The reactions were subjected to primer extension at 42°C for 45 min using 1 unit of MMLV-RT (Promega) and 0.5mM of dNTPs ...
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bioRxiv - Microbiology 2023Quote: ... The reactions were subjected to primer extension at 42°C for 45 min using 1 unit of MMLV-RT (Promega) and 0.5 mM of dNTPs ...
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bioRxiv - Molecular Biology 2020Quote: ... The PCR products were directly sequenced using PCR primers after purification with Gel Clean-up System (Promega Corporation, Madison, WI, USA). All sequencing reactions were done on ABI 3700 DNA automated sequencer with the BigDye chemistry (Applied Biosystems ...
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bioRxiv - Microbiology 2023Quote: ... The three PCR fragments were ligated by PCR using primers P1/P3 and cloned into pGEM®-T easy vector (Promega). Plasmid was then digested with NotI ...
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bioRxiv - Cell Biology 2020Quote: ... in 2× SSC supplemented with 3% (vol/vol) RNasin Ribonuclease inhibitor (Promega), 6% (vol/vol ...
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bioRxiv - Genetics 2024Quote: ... in 2x SSCT (0.1% Tween-20 in 2x SSC (Promega, Cat# V4261)) for 5 min at RT ...
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bioRxiv - Microbiology 2021Quote: qPCRs were performed with MLV SuMLV primers using a Power SYBR green PCR kit (Promega) and the QuantStudio 5 Real-Time PCR System (Applied Biosystems) ...
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bioRxiv - Plant Biology 2020Quote: The qRT-PCRs/qPCR reactions were set up using the GoTaq® qPCR Mastermix (Promega, Madison, Wisconsin, USA) according to the manufacturer’s instructions in a total volume of 15 μl ...
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bioRxiv - Genomics 2019Quote: ... We then set up quantitative PCR reactions with 5 µL GoTaq qPCR Master Mix (Promega, Madison, WI, USA), 1 µL each forward and reverse primer at 5 µM ...
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bioRxiv - Molecular Biology 2022Quote: ... Standard curves for each primer set were generated using serially diluted plasmid (for gAAV) or Human and Mouse Genomic DNA (for host) (Promega G1521 and G3091, respectively) and used for quantification ...
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bioRxiv - Molecular Biology 2023Quote: ... phosphorothioated and phosphorylated/phosphorothioated forward primers as well as a phosphorylated reverse primer were done in qPCR reaction mixtures including 1X reaction mixture of GoTaq 2-Step RT-qPCR kit (Promega, USA), 400 nM forward and reverse primers and in final volume of 20 μL ...
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bioRxiv - Molecular Biology 2020Quote: ... The isolated RNA was used for cDNA synthesis using the Access RT-PCR system (Promega) with AMV reverse transcriptase ...