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Citations for Promega :
101 - 150 of 2377 citations for ssc mir 17 RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2020Quote: ... The isolated RNA was used for cDNA synthesis using the Access RT-PCR system (Promega) with AMV reverse transcriptase ...
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bioRxiv - Plant Biology 2021Quote: ... RNA-isolation for RT-PCR analysis was done using the PureYield™ RNA kit (Promega). For expression analysis of HvTB2 in vrs4 background the vrs4.k mutant (GSHO 1986) ...
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bioRxiv - Microbiology 2022Quote: ... Viral RNA was quantified by real-time RT-qPCR (GoTaq 1-step qRt-PCR, Promega) using 3.8μL of extracted RNA and 6.2μL of RT-qPCR mix and standard fast cycling parameters ...
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bioRxiv - Neuroscience 2021Quote: ... The temporal expression of genes was characterized RT-PCR using GoTaq Master Mix (M712C, Promega) and oligonucleotide sequences are listed at https://zebrafishproject.ucsf.edu ...
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bioRxiv - Microbiology 2021Quote: ... Viral RNA was quantified by real-time RT-qPCR (GoTaq 1-step qRt-PCR, Promega) using 3.8µL of extracted RNA and 6.2µL of RT-qPCR mix and standard fast cycling parameters ...
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bioRxiv - Microbiology 2022Quote: ... Viral RNA was quantified by real-time RT-qPCR (GoTaq 1-step qRt-PCR, Promega) using 3.8 µL of extracted RNA and 6.2 µL of RT-qPCR mix and standard fast cycling parameters ...
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bioRxiv - Genomics 2019Quote: ... PCR was performed with gene-specific primer pairs (Table S15) using a DNA polymerase kit (Promega). The PCR conditions were 95 °C for 3 min ...
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bioRxiv - Microbiology 2021Quote: ... and primers using Wizard® SV Gel and PCR Clean-Up System (Promega, Madison, WI, USA). Cleaned amplified products were again run (5μl loaded ...
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bioRxiv - Microbiology 2019Quote: ... or mCherry were amplified by PCR (5’-CCGGGTACCATGGGCAGCAGCCATCATC and 5’-CGGGAATTCTTACTTGTACAGCTCGTCCAT primers; Pfu DNA polymerase, Promega) from the pRGrectac-NHis constructions (Fernández-Tresguerres et al. ...
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bioRxiv - Plant Biology 2022Quote: ... cDNAs were synthesized from 1.5 μg of total RNA with oligo(dT) primers using Moloney Murine Leukemia Virus Reverse Transcriptase MMLV-RT according to the manufacturer’s instructions (Promega, Madison, WI, USA). RT-qPCR was carried out in a Chromo4 system (Bio-Rad ...
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bioRxiv - Molecular Biology 2020Quote: ... or miR-324-3p.1 were amplified and inserted into pmirGLO (Promega) at the XhoI and XbaI sites ...
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bioRxiv - Cancer Biology 2020Quote: ... 2 µl of the RT product (diluted 1:5) was used for semi-quantitative PCR or qPCR reactions with Promega PCR Mix (Promega, Madison, Wisconsin, USA) and SYBR Green PCR Mix (Applied Biosystems ...
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bioRxiv - Cancer Biology 2021Quote: ... Real-time quantitative PCR (RT-qPCR) was performed with GoTaq qPCR Master Mix (Promega; Cat#: A6002). Gene expression analysis was calculated following normalization to PPAI using the comparative Ct (cycle threshold ...
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bioRxiv - Bioengineering 2021Quote: ... RNA (1 μg) was subjected to RT-PCR in accordance with the protocol provided by Promega. The transcripts were quantitated and normalized to the internal GAPDH control ...
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bioRxiv - Developmental Biology 2022Quote: ... the ARF3 coding region was amplified by RT-PCR and cloned into pGEM-T-easy (Promega). The resulting plasmid was digested with SpeI and transcribed with T7 RNA polymerase to generate the antisense probe ...
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bioRxiv - Microbiology 2019Quote: ... Ligation products were reverse transcribed into cDNA and amplified with the Access RT-PCR Kit (Promega) using a primer complementary to the 3’ adapter and a primer specific for the 5’-end sequence of transcripts initiated at the P1-tfoY promoter ...
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bioRxiv - Genomics 2021Quote: ... The RT-PCR experiments were conducted using the GoTaq G2 Hot Start Master mix (Promega #M7422). The PCR primers were listed in the Supplementary Figure S5 ...
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bioRxiv - Bioengineering 2021Quote: ... RNA (1 μg) was subjected to RT-PCR in accordance with the protocol provided by Promega. The transcripts were quantitated and normalized to the internal GAPDH control ...
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bioRxiv - Genetics 2021Quote: ... RT-PCR was performed by using GoTaq® DNA polymerase (Promega, 2800 Woods Hollow Road·Madison, USA) in an Applied Biosystems™ Veriti™ Thermal Cycler ...
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bioRxiv - Immunology 2022Quote: ... Target gene expression was determined by quantitative RT-PCR using GoTaq qPCR Master mix (Promega, A6002) on a QuantStudio 5 Real-Time PCR System (Applied Biosystems ...
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bioRxiv - Plant Biology 2024Quote: ... Real-time quantitative PCR (RT-qPCR) was performed using the GoTaq QPCR Master Mix (Promega, A6001) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... droplets were resuspended in 30 ml of room temperature 6X SSC (Promega, Madison, WI) and 1 ml perfluorooctanol (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2021Quote: ... For PCR genotyping with primers listed in Supplementary Table S5 and the GoTaq G2 DNA Polymerase (Promega) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... clones were screened by colony PCR with sabBFor and jhp0660R primers and Go Taq master mix (Promega). Dye-terminator sequencing using BigDye (Thermo Fisher ...
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bioRxiv - Plant Biology 2023Quote: ... AP1 (AT1G69120) cDNA was PCR-amplified using specific primers and inserted into XbaI/BamHI digested pSP64 (Promega) vector ...
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bioRxiv - Plant Biology 2020Quote: ... PCR products amplified by gene-specific RT-qPCR primers listed in Supplemental Table S1 were cloned in pGEM-T Easy vector (Promega, Madison, Wisconsin, USA) prior to their sequencing ...
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bioRxiv - Plant Biology 2021Quote: ... RT-PCR was performed with the GoTaq® Green Master Mix as per manufacturer’s instructions (Promega, USA). For RIP analysis ...
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bioRxiv - Biochemistry 2021Quote: Purified RT-PCR products were cloned in to pGEM vector using pGEM- T easy vector Systems (Promega) as described by manufacturer and transformed into heat- competent 10-Beta cells (NEB) ...
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bioRxiv - Molecular Biology 2022Quote: ... Complementary DNA (cDNA) was synthesized from total RNA using a RT-PCR kit (Promega, Madison, WI, USA) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2020Quote: ... 17 Synergy assessments were performed using CellTiter-Glo (Promega) as previously described26 with the following modifications ...
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bioRxiv - Cell Biology 2023Quote: ... Set of each dNTPs (U1130) (Promega); formaldehyde (P2031) ...
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bioRxiv - Microbiology 2021Quote: ... cDNA was synthesized using 5 μl of the eluent as template using the U3 antisense primer 5’ TGTGTGGTAGATCCACATCG-3’ and M-MLV RT RNase [H-] (Promega, WI. Cat. No. MR3681) following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2020Quote: ... Plates were incubated ∼18 hours at 37°C and individual white colonies screened for insert by colony PCR using primers M13_F (5’-ACGACGTTGTAAAACGACGGCCAGT-3’) and M13_R (5’-ATTTCACACAGGAAACAGCTATGACCA-3’) GoTaq DNA Polymerase (Promega). Reactions were separated by gel electrophoresis ...
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bioRxiv - Cell Biology 2020Quote: ... mutagenesis primers were used for PCR amplification of pRK7-FLAG-Rheb using pfu Turbo Polymerase (Promega, NSW, Australia) to induce specific mutations ...
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KIF24 controls the clustering of supernumerary centrosomes in pancreatic ductal adenocarcinoma cellsbioRxiv - Cell Biology 2022Quote: ... and amplified PCR products using primers listed in Table S1 were sub-cloned into pGEM-T Easy (Promega). Purified plasmid DNAs were sequenced using M13 primers.
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bioRxiv - Biochemistry 2021Quote: Linear DNA templates were generated by PCR using appropriate primers and transcribed into mRNA with T7 polymerase (Promega). In vitro translation was performed in rabbit reticulocyte lysate (Promega ...
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bioRxiv - Genetics 2019Quote: ... 0.8 µM of both the FAM labelled forward primer and TPP_M13R and 0.2 µM 5R_TPP using GoTaq® Long PCR Polymerase (Promega). A standard 60TD55 protocol was utilized (94°C denaturation for 30 s ...
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bioRxiv - Neuroscience 2021Quote: ... Random Primers (Promega), and Superscript III reverse transcriptase (Invitrogen ...
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bioRxiv - Genetics 2022Quote: ... Random Primers (Promega), and Superscript III reverse transcriptase (Invitrogen).
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bioRxiv - Cell Biology 2022Quote: ... random primers (Promega). The cDNA was diluted and used with TaqMan Fast Advanced Master Mix (Applied Biosciences ...
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bioRxiv - Neuroscience 2020Quote: ... RT-qPCR was performed on the ViiA 7 Real-Time PCR System using GoTaq qPCR master mix (Promega) according to the manufacturer’s protocols ...
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bioRxiv - Immunology 2021Quote: ... qRT-PCR was performed in 12 μL reactions using GoTaq Probe 1-step RT-qPCR System (Promega, US) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: Real-time PCR was carried out using the Promega GoTaq® Probe 1-Step RT-qPCR System (Promega) with Sarbecovirus-specific envelope gene primers from bat RNA samples as published by (Corman ...
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bioRxiv - Developmental Biology 2023Quote: ... RT-PCR for mRNA transcripts was performed by using the ImProm-II Reverse Transcription System kit (Promega A3800). Real Time PCR was performed with Applied Biosystems Fast SYBR Green Master Mix (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2023Quote: Real-time PCR was carried out using the Promega GoTaq® Probe 1-Step RT-qPCR System (Promega) with Sarbecovirus-specific envelope gene primers from bat RNA samples as published by (Corman ...
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bioRxiv - Biochemistry 2024Quote: ... using random primers and oligo-dT primers (3:1 mol) (Promega). The obtained cDNA was stored at −20 °C until further use.
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bioRxiv - Systems Biology 2022Quote: ... AMV RT (Promega) with 9 bp random primers was used for reverse transcription ...
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bioRxiv - Microbiology 2023Quote: ... AMV-RT (Promega) was used to reverse-transcribe the RNA-protein complex reaction at 30 °C for 1 h ...
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bioRxiv - Immunology 2022Quote: ... on memory CD8 T cells were quantified by qRT-PCR using a final 0.5 μM concentration of each primer and SYBR Green GoTaq qPCR Master Mix (Promega). Amplification was performed in a StepOne Plus Real time PCR system (Applied Biosystems) ...
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bioRxiv - Molecular Biology 2022Quote: ... The library was amplified for six PCR cycles with short primers followed by a ProNex size selection step (Promega) in a 1:2.95 (v/v ...