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Citations for Promega :
501 - 550 of 2354 citations for ssc mir 15a RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... 1 µM each primer and GoTaq polymerase reagent (Promega, Madison, WI, USA) were subjected to thermal cycling ...
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bioRxiv - Genomics 2023Quote: ... 200 nM primers and one unit of Taq polymerase (M829B, Promega, USA) was used for various fragment amplifications ...
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bioRxiv - Microbiology 2021Quote: ... Colony PCR was performed using PCR 2x Master Mix (Promega) with primers listed in Table 3.
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bioRxiv - Microbiology 2021Quote: ... Colony PCR was performed using PCR 2x Master Mix (Promega) with primers listed in Table S3.
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bioRxiv - Cell Biology 2023Quote: ... PCR reactions were prepared with GoTaq PCR Master Mix (Promega) following the manufacturer’s protocol ...
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bioRxiv - Evolutionary Biology 2019Quote: ... PCR (GoTaq, Promega) was used to amplify specific regions of the SOC ...
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bioRxiv - Cell Biology 2022Quote: ... PCR buffer (Promega) and MgCl2 (2.5 mM) ...
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bioRxiv - Microbiology 2023Quote: ... PCR cleanup (Promega), and quantification ...
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bioRxiv - Molecular Biology 2020Quote: ... Prior to the addition of 1 μl of MMLV-RT enzyme (Promega), the reaction was incubated at 65°C in order to denature double stranded RNA ...
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bioRxiv - Microbiology 2019Quote: ... Reverse transcription (RT) was performed using the M-MLV Reverse Transcriptase (Promega) and quantitative PCR (qPCR ...
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bioRxiv - Evolutionary Biology 2021Quote: ... The isolated RNA was reverse transcribed into cDNA with MLV RT (Promega), following the manufacturer’s protocol ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... GoTaq® Probe 1-Step RT-qPCR System was purchased from Promega. SARS-CoV-2 (2019-nCoV ...
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bioRxiv - Microbiology 2020Quote: ... The standard curve was generated using GoTaq 1-Step RT-qPCR (Promega).
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... GoTaq® Probe 1-Step RT-qPCR System was purchased from Promega. SARS-CoV-2 (2019- nCoV ...
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bioRxiv - Cell Biology 2021Quote: ... One µg RNA was reverse transcribed using the MLV RT kit (Promega). The GoTaq® qPCR Master Mix (Promega ...
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bioRxiv - Neuroscience 2021Quote: ... Proteins were then digested for 3h at RT with 1μg LysC (Promega) and then digested overnight at 37°C with 2μg of Trypsin ...
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bioRxiv - Molecular Biology 2022Quote: ... The GoTaq® qPCR and RT-qPCR Systems kit (Promega, Beijing, China) was used to quantify the transcript abundance on the ABI 7500 real-time PCR system (Applied Biosystems) ...
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bioRxiv - Neuroscience 2023Quote: ... and reverse transcribed into cDNA using the Promega RT Kit (Promega, A3500). RT-qPCR was performed using specific primer sequences for FOXG1 (F-CGTTCAGCTACAACGCGCTCA ...
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bioRxiv - Genomics 2023Quote: ... This was followed by addition of 1 μL M-MLV RT (Promega) to initiate reverse transcription in a 20 μL reaction of the M-MLV buffer ...
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bioRxiv - Developmental Biology 2024Quote: ... RT-qPCR was performed using GoTaq qPCR SYBR Green Master Mix (Promega). GAPDH was used as an endogenous control (primer details are listed in Supplementary Table 3).
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bioRxiv - Microbiology 2022Quote: ... PCR was conducted in Promega PCR master mix (Promega, Madison, WI). SYBR green-based qPCR and probe-based qPCR was conducted in SsoAdvanced universal SYBR green supermix (Bio-Rad Canada ...
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bioRxiv - Developmental Biology 2022Quote: ... PCR products were purified using PCR purification kit (Promega, WI, US), and ligated into PCR II Topo-vector (Invitrogen ...
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bioRxiv - Microbiology 2023Quote: ... PCR was performed using GoTaq® Long PCR Master mix (Promega) and each reaction consisted of ...
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bioRxiv - Cell Biology 2020Quote: ... total RNA was annealed with 0.1 mg/ml oligo(dT)15 primer (Promega) and cDNA synthesis was performed with M-MLV Reverse Transcriptase (Promega ...
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bioRxiv - Molecular Biology 2021Quote: 500 ng of total RNA was reverse transcribed with Random Primers (Promega C1181) and MMLV Reverse Transcriptase (Sigma M1302 ...
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bioRxiv - Molecular Biology 2021Quote: ... primers (Supplementary Table 1) and GoTaq® G2 Flexi DNA polymerase (M7801, Promega). Resulting PCR products were purified on columns (Isolate II PCR Kit (BIO-52059 ...
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bioRxiv - Physiology 2019Quote: ... and then converted to cDNA using MMLV Reverse Transcriptase with random primers (Promega). Quantitative RT-PCR was carried out with either TaqMan™ Universal PCR Master Mix or SYBR Green PCR master mix on the QuantStudio 7 Flex Real time PCR system (Applied Biosystems) ...
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bioRxiv - Microbiology 2020Quote: ... cDNA was generated using oligo(dT)20 primer (GoScript Reverse Transcription System, Promega), before RT-qPCR using primers for socs3 and gapdh ...
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bioRxiv - Microbiology 2021Quote: ... 0.32 μM of each primer and 0.5U of GoTaq® DNA Polymerase (Promega) in a total volume of 25 μL ...
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bioRxiv - Genomics 2022Quote: ... The primers were commercially synthesized (IDT) and tested on human genomic DNA (Promega) to confirm generation of only one amplicon product at the expected size ...
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bioRxiv - Microbiology 2022Quote: ... 0.32 μM of each primer and 0.63U of GoTaq® DNA Polymerase (Promega) in a total volume of 25 μL ...
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bioRxiv - Molecular Biology 2019Quote: ... 500 ng were used in a reverse transcription reaction with Random Primers (Promega) and SuperScript® II Reverse Transcriptase (Invitrogen) ...
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bioRxiv - Cell Biology 2019Quote: ... using primers specific to individual genes and GoTaq® qPCR Master Mix (Promega). The incubation and cycling conditions were set as described in the kit and the plates were analysed in a StepOnePlus Real-Time PCR System (Thermo Scientific).
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bioRxiv - Genetics 2020Quote: ... 1 uM of each primer and 1X of reaction buffer (Promega, Madison WI). Thermal cycles were set up to an initial denaturing step of 95 C ...
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bioRxiv - Synthetic Biology 2020Quote: ... 25 pmol of each primer and 1 U of GoTaq DNA polymerase (Promega). Reactions were run by an initial denaturalization (5 min ...
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bioRxiv - Microbiology 2021Quote: 500 ng of total RNA was reverse-transcribed using random hexamer primers (Promega) and Improm-II reverse transcriptase (Promega ...
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bioRxiv - Developmental Biology 2020Quote: ... 1μg of RNA was reverse transcribed with oligo(dT)15 Primers (Promega C1101)] using SuperScript III Reverse Transcriptase (Invitrogen ...
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bioRxiv - Genetics 2021Quote: ... and reverse transcribed using an oligo-dT primer and GoScriptTM Reverse Transcriptase (Promega). The resulting transcribed cDNA was amplified for 29 cycles with primers specific for the alanine aminotransferase (Aat ...
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bioRxiv - Evolutionary Biology 2021Quote: ... cDNA synthesis was performed with 0.5 µg of RNA using random primers (Promega) and the MMLV reverse transcriptase enzyme (Promega ...
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bioRxiv - Immunology 2021Quote: cDNA was prepared from 0.1 µg of total RNA using Random Primers (Promega) and M-MLV Reverse Transcriptase (Promega) ...
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bioRxiv - Molecular Biology 2022Quote: ... 1 μg was used in a reverse transcription reaction with Random Primers (Promega) and RevertAid Reverse Transcriptase (ref ...
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bioRxiv - Physiology 2022Quote: ... 0.32 µM of each primer and 0.5U of GoTaq® DNA Polymerase (Promega) in a total volume of 25 µL ...
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bioRxiv - Microbiology 2022Quote: ... Briefly 500 ng of total RNA were reverse transcribed using random primers (Promega) with the ImProm-II reverse transcriptase (Promega ...
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bioRxiv - Molecular Biology 2022Quote: ... and reverse transcribed with random hexamer primers using MMLV reverse transcriptase (Promega, M1701) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2023Quote: ... Reverse Transcriptase reaction was performed with 1µg RNA with random primers (C1181, Promega), dNTPs (N0447S ...
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bioRxiv - Cell Biology 2023Quote: ... and cDNA synthesis using the GoScript Reverse Transcription Mix Random Primers (A2800, Promega) were done according to the instructions of the manufacturer ...
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bioRxiv - Cell Biology 2023Quote: ... and primers were removed using the ProNex Size-Selective Purification System (Promega, NG2001) in a 1:2.4 (v/v ...
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bioRxiv - Molecular Biology 2024Quote: ... and primers were removed using the ProNex Size-Selective Purification System (Promega, NG2001) in a 1:2.4 (v/v ...
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bioRxiv - Biochemistry 2024Quote: ... and primers were removed using the ProNex Size-Selective Purification System (Promega, NG2001) in a 1:2.4 (v/v ...
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bioRxiv - Biochemistry 2020Quote: Transcription assays were set up using the HeLa Scribe Nuclear Extract in vitro Transcription system (Promega, catalog # E3110) following the manufacture’s protocol with modifications ...