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Citations for Promega :
651 - 700 of 5334 citations for rno mir 143 Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: ... DNA was eluted from the beads using the elution buffer (1% SDS and 100 mM NaHCO3) and purified by PCR purification kit (Promega). The final products were analyzed by quantitative real-time PCR with the primers listed below:
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bioRxiv - Developmental Biology 2021Quote: ... The PCR amplified inserts were run on a 1% agarose gel and purified using the Wizard SV Gel and PCR Clean-Up System kit (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2021Quote: ... and then excised and purified using the Wizard® SV Gel and PCR Clean-Up System kit (Promega, Madison, WI) according to manufacturer’s protocol ...
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bioRxiv - Synthetic Biology 2021Quote: ... Amplification products were checked on a 1% agarose gel and were purified using the Wizard PCR clean-up kit (Promega). DNA concentration and purity were measured using a ND-1000 UV-Vis Spectrophotometer (Nanodrop Technologies).
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bioRxiv - Plant Biology 2020Quote: ... Gene PCR products of 200-300 bp were labelled with 32P-dCTP following the prime-a-gene labelling kit instructions (Promega) and used as probes (Supplementary Table 1) ...
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bioRxiv - Microbiology 2020Quote: ... PCR reaction mixtures were solved in 1% (w/v) agarose gel and purified by means of Wizard SV gel and PCR Clean Up kits (Promega). The amplified gene was treated with Taq polymerase (Fermentas ...
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bioRxiv - Evolutionary Biology 2022Quote: ... DNA was purified from the gel fragments using the Wizard SV Gel and PCR Clean Up kit (Promega, Fitchburg, USA) according to the manufacturer’s protocol.
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bioRxiv - Physiology 2022Quote: ... cDNA was synthesized by using qScript cDNA Synthesis Kit (QuantaBio, Beverly, MA) and PCR was performed with GoTaq qPCR Master Mix (Promega) with HPRT as the control gene ...
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bioRxiv - Plant Biology 2022Quote: ... Hybridization probes were generated by PCR amplifi cation using gene specifi c primers (Table S1) and radiolabeled with the Prime A Gene labeling kit (Promega) following the recommendations of the manufacturer.
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bioRxiv - Microbiology 2023Quote: ... higginsianum genomic DNA used as PCR templates were isolated by Maxwell RSC Plant DNA Kit with Maxwell RSC 48 instrument (Promega) following the manufacturer’s protocol ...
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bioRxiv - Genetics 2023Quote: ... After a second purification using a Zymo DNA Clean and Concentrator kit the PCR product was used as a template in a T7 RiboMAX express RNAi System (Promega) following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: RT-qPCRs were performed with MLV SU or 2-LTR primers (Table 1) using a Power SYBR green PCR kit (Promega) and the QuantStudio 5 real-time PCR system (Applied Biosystems) ...
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bioRxiv - Biochemistry 2023Quote: ... PCR reaction mixtures were solved in 1% (w/v) agarose gel and purified using Wizard SV gel & PCR Clean-Up kits (Promega). Then ...
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bioRxiv - Microbiology 2023Quote: ... Gene expression was assessed by qPCR using primers listed in the supplemental material Table S6 and the GO Taq PCR kit (Promega). Gene expression was quantified using SYBR Green (Roche ...
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bioRxiv - Developmental Biology 2023Quote: ... Lysates were diluted 1:10 with Milli-Q water and processed for PCR using GoTaq G2 Flexi DNA Polymerase kit (Promega). Reactions were placed in a thermocycler on a touchdown program ...
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bioRxiv - Bioengineering 2023Quote: ... PCR products were confirmed on a 0.8% agarose gel and purified using Wizard® SV Gel and PCR Clean-Up kit (Promega). DNA fragments were quantified using Nanodrop (ThermoFisher Scientific) ...
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bioRxiv - Microbiology 2023Quote: ... core and modified histones and transcription factors at the HIV-1 LTR was assessed by quantitative PCR using primers spanning the full promoter (Table 1) with GoTaq qPCR Master mix kit (Promega) in a CFX Connect Real-Time PCR thermocycler (BioRad) ...
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bioRxiv - Developmental Biology 2024Quote: ... Digoxigenin-labelled or Fluorescein-labelled mRNA probes were either prepared as PCR probes with primers listed in Table S4 using the Kit GoTaq green master mix (Promega) or as described (Orgeur et al. ...
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bioRxiv - Molecular Biology 2024Quote: ... Tail DNA from offspring was genotyped for the presence of the BAC transgene by analyzing 100 ng of tail DNA with the GoTaq PCR reaction kit (Promega) according to the manufacturer’s instruction by adding forward primer 5′-GAGGCTCGGGTGCGGCAGCTC −3′ and reverse primer 5′-GTGCAGTGTGAGCACCTGCTGTC-3′ for PCR with an initial denaturation for 3 min at 95 °C ...
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bioRxiv - Microbiology 2020Quote: ... PCR products were purified using the Wizard PCR Preps DNA Purification System (Promega) and DNA sequencing was performed using primer 2569 ...
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bioRxiv - Microbiology 2023Quote: ... PCR reactions were carried out using a 2x GoTaq PCR master mix (Promega), biotin-labeled M13F and biotin-labeled M13R primers ...
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bioRxiv - Cancer Biology 2024Quote: ... and qRT-PCR was performed using the GoTaq qRT-PCR master mix (Promega) with the ViiA7 Real Time PCR system (Applied Biosystems) ...
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bioRxiv - Pathology 2023Quote: ... USA) using SYBR Green PCR or TaqMan Universal PCR master mixes (Go Taq Probe PCR Master Mix; Promega, A600A or A610A, respectively). The following TaqMan probes were used ...
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bioRxiv - Evolutionary Biology 2022Quote: ... TUNEL staining was done using DeadEnd Fluorometric TUNEL detection system (REF-G3250, Promega, USA). For positive control ...
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bioRxiv - Microbiology 2021Quote: ... diluted 1:2 with PBS and read on a GloMax Multi+ Detection System (Promega). CSV files were exported onto a USB flash drive for analysis.
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bioRxiv - Pathology 2021Quote: ... Fluorescence of the lysates was then analyzed using a multi detection plate reader (Promega Glomax Multi Detection System ...
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bioRxiv - Cancer Biology 2020Quote: ... Luminescence from both luciferase reactions was measured with the Glomax Multi-detection System (Promega). Firefly luminescence values were normalized against Renilla luminescence values ...
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bioRxiv - Developmental Biology 2022Quote: ... Fluorescence in stained cells were detected with a GloMax Discover Multimode Detection System (Promega) using 520 nm excitation and emission at 590 nm ...
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bioRxiv - Microbiology 2022Quote: ... diluted 1:2 with PBS and read on a GloMax Multi+ Detection System (Promega).
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bioRxiv - Molecular Biology 2019Quote: ... The translated protein was analyzed using a Transcend Non-Radioactive Translation Detection System (Promega).
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bioRxiv - Immunology 2021Quote: ... and the luminescence signal was recorded using the GloMax-Multi Detection System (Promega, WI).
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bioRxiv - Microbiology 2019Quote: ... HiBiT signal was quantified by mixing samples with HiBiT Extracellular Detection Reagent (Promega, N2421) at a 1:1 ratio and reading luminescence with a Synergy HTX plate reader ...
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bioRxiv - Immunology 2021Quote: ... ADCC was measured with Nano-Glo HiBiT Extracellular Detection System (Promega; Cat. Nr.: N2421) using a luminometer (Integration Time 00:30).
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bioRxiv - Immunology 2021Quote: ... Cells were lysed and subjected to luciferase activity detection according to manufacturer instructions (Promega). All reactions were performed in triplicate.
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bioRxiv - Immunology 2022Quote: ... ADCC was measured with Nano-Glo HiBiT Extracellular Detection System (Promega; Cat. Nr.: N2421) using a luminometer (Integration Time 00:30) ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Luciferase and renilla activity were then measured using the Glomax multi+ detection system (Promega). Luciferase activity values were standardized by the renilla activity values and background activity values as determined by measuring luminescence from the pGL3-Basic[minP] plasmid with no insert ...
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bioRxiv - Cell Biology 2023Quote: Luminescence was assessed with the Nano-Glo® HiBiT Lytic Detection System (Promega, N3040) in 384-well plate format and in technical triplicates ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: Luminescence was assessed with the Nano-Glo® HiBiT Lytic Detection System (Promega, N3040) in 384-well plate format and in technical triplicates ...
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bioRxiv - Cancer Biology 2023Quote: ... Luminescence was measured using a Glomax Multi Plus Detection System (Promega, Madison, WI, USA), and caspase 3/7 activities was normalized by the number of cells ...
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bioRxiv - Microbiology 2023Quote: ... The HiBiT value measured by Nano Glo HiBiT lytic detection system (Promega, Cat# N3040) indicates the amount of p24 HIV-1 antigen ...
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bioRxiv - Physiology 2023Quote: ... Chemiluminescent detection of in vitro translated protein was performed following the manufacturer’s protocol (Promega).
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bioRxiv - Biochemistry 2024Quote: ... the blotted proteins were developed using Nano-Glo® In-Gel Detection System (Promega) and scanned with a ChemiDoc MP Imaging System (Bio-Rad).
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bioRxiv - Evolutionary Biology 2019Quote: ... First strand cDNA was synthesized using 5μg pure RNA and M-MLV RT (Promega). qPCR was performed using pure cDNA and qPCR kit (Agilent technologies) ...
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bioRxiv - Microbiology 2021Quote: ... USA) and 10 μl of GoTaq 1-Step RT-qPCR (Promega, Madison, WI, USA). Thermal cycling was performed at 50°C for 15min for reverse transcription ...
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bioRxiv - Cancer Biology 2020Quote: ... Plates were incubated for 1 h at RT and luminescence recorded (Glowmax Luminometer, Promega) and then compared to Glucose/Lactate standards to determine relevant concentrations.
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bioRxiv - Microbiology 2021Quote: ... incubated for 1 h at RT and analysed using the GloMax multidetection system (Promega). Parasitaemia was used to normalise parasite density between samples and replicates ...
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bioRxiv - Cell Biology 2021Quote: ... cDNA libraries were generated using M-MLV RT [H-] Point mutant (Promega, WI, USA) according to the manufacturer’s directions ...
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bioRxiv - Molecular Biology 2020Quote: ... Reverse transcription was performed with random hexamer primers using an MMLV-RT platform (Promega) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2022Quote: ... 10 μL of 4X concentrated Realtime-Glo (RT-Glo) reagent (Promega, Madison, WI, USA) diluted in antibiotic-free medium was dispensed ...
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bioRxiv - Microbiology 2021Quote: ... cDNA was synthesized at 42 °C for 60 min with M-MLV-RT (Promega) using gene-specific primer SP1_nbdA ...