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Citations for Promega :
101 - 150 of 2350 citations for rno mir 125b 5p RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: ... Standard curves for each primer set were generated using serially diluted plasmid (for gAAV) or Human and Mouse Genomic DNA (for host) (Promega G1521 and G3091, respectively) and used for quantification ...
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bioRxiv - Molecular Biology 2023Quote: ... phosphorothioated and phosphorylated/phosphorothioated forward primers as well as a phosphorylated reverse primer were done in qPCR reaction mixtures including 1X reaction mixture of GoTaq 2-Step RT-qPCR kit (Promega, USA), 400 nM forward and reverse primers and in final volume of 20 μL ...
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bioRxiv - Molecular Biology 2020Quote: ... The isolated RNA was used for cDNA synthesis using the Access RT-PCR system (Promega) with AMV reverse transcriptase ...
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bioRxiv - Plant Biology 2021Quote: ... RNA-isolation for RT-PCR analysis was done using the PureYield™ RNA kit (Promega). For expression analysis of HvTB2 in vrs4 background the vrs4.k mutant (GSHO 1986) ...
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bioRxiv - Microbiology 2022Quote: ... Viral RNA was quantified by real-time RT-qPCR (GoTaq 1-step qRt-PCR, Promega) using 3.8μL of extracted RNA and 6.2μL of RT-qPCR mix and standard fast cycling parameters ...
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bioRxiv - Neuroscience 2021Quote: ... The temporal expression of genes was characterized RT-PCR using GoTaq Master Mix (M712C, Promega) and oligonucleotide sequences are listed at https://zebrafishproject.ucsf.edu ...
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bioRxiv - Microbiology 2021Quote: ... Viral RNA was quantified by real-time RT-qPCR (GoTaq 1-step qRt-PCR, Promega) using 3.8µL of extracted RNA and 6.2µL of RT-qPCR mix and standard fast cycling parameters ...
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bioRxiv - Microbiology 2022Quote: ... Viral RNA was quantified by real-time RT-qPCR (GoTaq 1-step qRt-PCR, Promega) using 3.8 µL of extracted RNA and 6.2 µL of RT-qPCR mix and standard fast cycling parameters ...
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bioRxiv - Genomics 2019Quote: ... PCR was performed with gene-specific primer pairs (Table S15) using a DNA polymerase kit (Promega). The PCR conditions were 95 °C for 3 min ...
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bioRxiv - Microbiology 2021Quote: ... and primers using Wizard® SV Gel and PCR Clean-Up System (Promega, Madison, WI, USA). Cleaned amplified products were again run (5μl loaded ...
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bioRxiv - Microbiology 2019Quote: ... or mCherry were amplified by PCR (5’-CCGGGTACCATGGGCAGCAGCCATCATC and 5’-CGGGAATTCTTACTTGTACAGCTCGTCCAT primers; Pfu DNA polymerase, Promega) from the pRGrectac-NHis constructions (Fernández-Tresguerres et al. ...
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bioRxiv - Plant Biology 2022Quote: ... cDNAs were synthesized from 1.5 μg of total RNA with oligo(dT) primers using Moloney Murine Leukemia Virus Reverse Transcriptase MMLV-RT according to the manufacturer’s instructions (Promega, Madison, WI, USA). RT-qPCR was carried out in a Chromo4 system (Bio-Rad ...
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bioRxiv - Molecular Biology 2020Quote: ... or miR-324-3p.1 were amplified and inserted into pmirGLO (Promega) at the XhoI and XbaI sites ...
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bioRxiv - Cancer Biology 2020Quote: ... 2 µl of the RT product (diluted 1:5) was used for semi-quantitative PCR or qPCR reactions with Promega PCR Mix (Promega, Madison, Wisconsin, USA) and SYBR Green PCR Mix (Applied Biosystems ...
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bioRxiv - Cancer Biology 2021Quote: ... Real-time quantitative PCR (RT-qPCR) was performed with GoTaq qPCR Master Mix (Promega; Cat#: A6002). Gene expression analysis was calculated following normalization to PPAI using the comparative Ct (cycle threshold ...
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bioRxiv - Bioengineering 2021Quote: ... RNA (1 μg) was subjected to RT-PCR in accordance with the protocol provided by Promega. The transcripts were quantitated and normalized to the internal GAPDH control ...
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bioRxiv - Developmental Biology 2022Quote: ... the ARF3 coding region was amplified by RT-PCR and cloned into pGEM-T-easy (Promega). The resulting plasmid was digested with SpeI and transcribed with T7 RNA polymerase to generate the antisense probe ...
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bioRxiv - Microbiology 2019Quote: ... Ligation products were reverse transcribed into cDNA and amplified with the Access RT-PCR Kit (Promega) using a primer complementary to the 3’ adapter and a primer specific for the 5’-end sequence of transcripts initiated at the P1-tfoY promoter ...
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bioRxiv - Genomics 2021Quote: ... The RT-PCR experiments were conducted using the GoTaq G2 Hot Start Master mix (Promega #M7422). The PCR primers were listed in the Supplementary Figure S5 ...
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bioRxiv - Bioengineering 2021Quote: ... RNA (1 μg) was subjected to RT-PCR in accordance with the protocol provided by Promega. The transcripts were quantitated and normalized to the internal GAPDH control ...
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bioRxiv - Genetics 2021Quote: ... RT-PCR was performed by using GoTaq® DNA polymerase (Promega, 2800 Woods Hollow Road·Madison, USA) in an Applied Biosystems™ Veriti™ Thermal Cycler ...
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bioRxiv - Immunology 2022Quote: ... Target gene expression was determined by quantitative RT-PCR using GoTaq qPCR Master mix (Promega, A6002) on a QuantStudio 5 Real-Time PCR System (Applied Biosystems ...
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bioRxiv - Plant Biology 2024Quote: ... Real-time quantitative PCR (RT-qPCR) was performed using the GoTaq QPCR Master Mix (Promega, A6001) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... For PCR genotyping with primers listed in Supplementary Table S5 and the GoTaq G2 DNA Polymerase (Promega) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... clones were screened by colony PCR with sabBFor and jhp0660R primers and Go Taq master mix (Promega). Dye-terminator sequencing using BigDye (Thermo Fisher ...
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bioRxiv - Plant Biology 2023Quote: ... AP1 (AT1G69120) cDNA was PCR-amplified using specific primers and inserted into XbaI/BamHI digested pSP64 (Promega) vector ...
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bioRxiv - Plant Biology 2020Quote: ... PCR products amplified by gene-specific RT-qPCR primers listed in Supplemental Table S1 were cloned in pGEM-T Easy vector (Promega, Madison, Wisconsin, USA) prior to their sequencing ...
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bioRxiv - Plant Biology 2021Quote: ... RT-PCR was performed with the GoTaq® Green Master Mix as per manufacturer’s instructions (Promega, USA). For RIP analysis ...
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bioRxiv - Biochemistry 2021Quote: Purified RT-PCR products were cloned in to pGEM vector using pGEM- T easy vector Systems (Promega) as described by manufacturer and transformed into heat- competent 10-Beta cells (NEB) ...
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bioRxiv - Molecular Biology 2022Quote: ... Complementary DNA (cDNA) was synthesized from total RNA using a RT-PCR kit (Promega, Madison, WI, USA) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2023Quote: ... Set of each dNTPs (U1130) (Promega); formaldehyde (P2031) ...
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bioRxiv - Microbiology 2021Quote: ... cDNA was synthesized using 5 μl of the eluent as template using the U3 antisense primer 5’ TGTGTGGTAGATCCACATCG-3’ and M-MLV RT RNase [H-] (Promega, WI. Cat. No. MR3681) following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2020Quote: ... Plates were incubated ∼18 hours at 37°C and individual white colonies screened for insert by colony PCR using primers M13_F (5’-ACGACGTTGTAAAACGACGGCCAGT-3’) and M13_R (5’-ATTTCACACAGGAAACAGCTATGACCA-3’) GoTaq DNA Polymerase (Promega). Reactions were separated by gel electrophoresis ...
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bioRxiv - Cell Biology 2020Quote: ... mutagenesis primers were used for PCR amplification of pRK7-FLAG-Rheb using pfu Turbo Polymerase (Promega, NSW, Australia) to induce specific mutations ...
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KIF24 controls the clustering of supernumerary centrosomes in pancreatic ductal adenocarcinoma cellsbioRxiv - Cell Biology 2022Quote: ... and amplified PCR products using primers listed in Table S1 were sub-cloned into pGEM-T Easy (Promega). Purified plasmid DNAs were sequenced using M13 primers.
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bioRxiv - Biochemistry 2021Quote: Linear DNA templates were generated by PCR using appropriate primers and transcribed into mRNA with T7 polymerase (Promega). In vitro translation was performed in rabbit reticulocyte lysate (Promega ...
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bioRxiv - Genetics 2019Quote: ... 0.8 µM of both the FAM labelled forward primer and TPP_M13R and 0.2 µM 5R_TPP using GoTaq® Long PCR Polymerase (Promega). A standard 60TD55 protocol was utilized (94°C denaturation for 30 s ...
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bioRxiv - Neuroscience 2021Quote: ... Random Primers (Promega), and Superscript III reverse transcriptase (Invitrogen ...
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bioRxiv - Genetics 2022Quote: ... Random Primers (Promega), and Superscript III reverse transcriptase (Invitrogen).
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bioRxiv - Cell Biology 2022Quote: ... random primers (Promega). The cDNA was diluted and used with TaqMan Fast Advanced Master Mix (Applied Biosciences ...
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bioRxiv - Neuroscience 2020Quote: ... RT-qPCR was performed on the ViiA 7 Real-Time PCR System using GoTaq qPCR master mix (Promega) according to the manufacturer’s protocols ...
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bioRxiv - Immunology 2021Quote: ... qRT-PCR was performed in 12 μL reactions using GoTaq Probe 1-step RT-qPCR System (Promega, US) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: Real-time PCR was carried out using the Promega GoTaq® Probe 1-Step RT-qPCR System (Promega) with Sarbecovirus-specific envelope gene primers from bat RNA samples as published by (Corman ...
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bioRxiv - Developmental Biology 2023Quote: ... RT-PCR for mRNA transcripts was performed by using the ImProm-II Reverse Transcription System kit (Promega A3800). Real Time PCR was performed with Applied Biosystems Fast SYBR Green Master Mix (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2023Quote: Real-time PCR was carried out using the Promega GoTaq® Probe 1-Step RT-qPCR System (Promega) with Sarbecovirus-specific envelope gene primers from bat RNA samples as published by (Corman ...
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bioRxiv - Biochemistry 2024Quote: ... using random primers and oligo-dT primers (3:1 mol) (Promega). The obtained cDNA was stored at −20 °C until further use.
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bioRxiv - Systems Biology 2022Quote: ... AMV RT (Promega) with 9 bp random primers was used for reverse transcription ...
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bioRxiv - Microbiology 2023Quote: ... AMV-RT (Promega) was used to reverse-transcribe the RNA-protein complex reaction at 30 °C for 1 h ...
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bioRxiv - Immunology 2022Quote: ... on memory CD8 T cells were quantified by qRT-PCR using a final 0.5 μM concentration of each primer and SYBR Green GoTaq qPCR Master Mix (Promega). Amplification was performed in a StepOne Plus Real time PCR system (Applied Biosystems) ...
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bioRxiv - Molecular Biology 2022Quote: ... The library was amplified for six PCR cycles with short primers followed by a ProNex size selection step (Promega) in a 1:2.95 (v/v ...