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Citations for Promega :
1 - 50 of 1445 citations for pVectOZ GFP Transfection control since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... and a membrane GFP (75 ng as control for transfection) using FuGENE HD transfection reagent (1µg DNA/1µL FuGENE) (Promega, WI, USA). Next ...
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bioRxiv - Molecular Biology 2020Quote: ... GFP-FOXR1 or GFP-M280L plasmids using FuGENE6 transfection reagent (Promega) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2019Quote: ... Virus-like particles (VLPs) containing the GFP control or targeting sgRNAs were generated by transfection of HEK293 cells with FuGene (Promega). VLPs were collected from culture supernatant ...
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bioRxiv - Biochemistry 2020Quote: GFP plasmid transfections were conducted using Fugene HD (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: ... and control vector using ViaFect Transfection Reagent (Promega, Madison, WI).
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bioRxiv - Molecular Biology 2021Quote: XAB2-GFP and RNAP2-GFP stably expressing cell lines were produced by transfecting XAB2-GFP or RNAP2-GFP using FuGENE® 6 Transfection Reagent (Promega) according to the manufacturer’ protocol ...
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bioRxiv - Cell Biology 2023Quote: ... Either GFP-VCP-A232E or GFP-FUS-R495X were transfected into cells using ViaFect transfection reagent (Promega) 24 h prior to the start of the experiment ...
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bioRxiv - Cell Biology 2020Quote: ... Plasmid transfections for GFP imaging were achieved using Fugene HD (Promega, # E2311) as per the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2020Quote: ... Fluc reporter plasmids and pRL-TK renilla luciferase (rluc) transfection control plasmid (Promega) were co-transfected into these cells using jetPRIME® transfection reagent (Illkirch-Graffenstaden ...
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bioRxiv - Microbiology 2020Quote: Replication competent HIV-1 and VSV-G pseudotyped HIV-1 GFP vectors were produced by transfection of HEK293T cells in T150 flasks using Fugene 6 transfection reagent (Promega) according to the manufacturer’s instructions ...
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Salmonella actively modulates TFEB in murine macrophages in a growth-phase and time-dependent mannerbioRxiv - Cell Biology 2023Quote: ... and then transfected with the plasmid encoding GFP-TFEB or mCherry-GFP-LC3 using FuGene HD transfection reagent (Promega, WI), following manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... The 293T derivative cell line BOSC23 was transfected with pCL-Eco and pMX-IRES-GFP or pMX-AID-ER-IRES-GFP retroviral vectors using FuGENE® HD Transfection Reagent (Promega) to generate viral particle-containing supernatants ...
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bioRxiv - Molecular Biology 2023Quote: Transient plasmid WT-PolK GFP 54 transfections were performed using FuGene six reagent (Promega), according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2019Quote: Transfection of the GFP-LC3-mCherry reporter construct was carried out using FuGene HD (Promega) with 800 ng of DNA (ratio 5.2:1 ...
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bioRxiv - Biochemistry 2023Quote: ... AGO1/2/3-/- cells were transfected with WT-AGO2-GFP or NES-AGO2-GFP plasmids with OptiMEM and FuGENE HD Transfection Reagent (Promega, Cat. No. E2311) with media change to full media at 24 hours post-transfection ...
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bioRxiv - Cancer Biology 2019Quote: ... The luminescence values were transfection normalized with the internal control pRL-TK (50 ng, Renilla Luciferase; Promega). Experiments were performed with three biological replicates.
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bioRxiv - Molecular Biology 2020Quote: ... 293T cells were transfected in 12 well plates with 1 ug of pcDNA3.4 POLGARF or pcDNA3.4 Timer (as a negative control) with Fugene transfection reagent (Promega) according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were co-transfected with 50 ng of the Renilla luciferase transfection control plasmid pRL-CMV (Promega #E2261) and 1 μg of either the ERα reporter plasmid XETL (here referred to as ERE-Luc ...
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bioRxiv - Cell Biology 2021Quote: HeLa cells were plated at 100,000cells in 2 ml media onto 35mm MatTek glass bottom dishes and transfected with 0.9µg RUSH plasmid and 0.1µg eGFP-GalT or LAMP1-GFP using 100µl Opti-MEM and 3µl Fugene 6 transfection reagent (Promega) one day before imaging ...
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bioRxiv - Zoology 2020Quote: ... K562 cells were transfected with 0.1 μg of Renilla luciferase expression plasmid pRL-TK (internal control for normalizing transfection efficiency; Promega) and 0.4 μg of abcb4 promoter construct ...
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bioRxiv - Immunology 2023Quote: ... pI.18-3xFlag-myRIG-I or pI.18-3xFlag-roRIG-I together with transfection control pLR-SV40-Renilla (Promega) and either of the reporter plasmids ISG54-Luc (69) ...
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bioRxiv - Cell Biology 2020Quote: HeLa cells were transfected with pSpCas9(BB)-2A-GFP encoding gRNAs using FuGENE HD Transfection Reagent (E2311; Promega). Two days after transfection ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were transfected with 8 µg of pcDNA3.1-GFP-mNLS-K145Q using Fugene 6 Transfection reagent (Promega, E2692) per manufacturer protocol ...
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bioRxiv - Microbiology 2020Quote: ... transfections were performed in triplicate by co-transfection of the pHMRluc Rem-responsive construct and a Rem-non-responsive firefly luciferase reporter construct (pGL3-Control; Promega), in the presence and absence of a Rem-expression plasmid ...
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bioRxiv - Genomics 2022Quote: ... Firefly (transfection control) and Renilla (target) luciferase activities were sequentially measured using the Dual-Glo Luciferase Assay System (Promega E2920) and detected on a Spectramax M plate reader (Molecular Devices) ...
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bioRxiv - Immunology 2023Quote: ... pI.18-3xFlag-myRIG-I or pI.18-3xFlag-roRIG-I (25 ng/well each) together with transfection control pLR-SV40-Renilla (Promega) (50 ng/well ...
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bioRxiv - Genetics 2024Quote: ... Cells were co-transfected after 24 hours with 50 ng of the CNTNAP5 luciferase reporter constructs along with 10 ng of pRL-TK Renilla luciferase internal control using FuGENE HD transfection reagent (Promega). At 48 hours post-transfection ...
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bioRxiv - Cancer Biology 2022Quote: The 4T1-mScarlet and 67NR-GFP cell lines were generated by transfection using a 1:4 ratio of plasmid DNA:FugeneHD reagent (Promega), according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: ... and 0.4 pmol/well of hairpin constructs (pscAAV-GFP-shFoxP1 or shCtrl) using FuGENE 6 Transfection Reagent (#E2691, Promega) according to the manufacturer’s instructions ...
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bioRxiv - Zoology 2020Quote: ... pGL3-control plasmid (positive control; Promega), or pGL3-enhancer plasmid (negative control ...
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Receptor-like role for PQLC2 amino acid transporter in the lysosomal sensing of cationic amino acidsbioRxiv - Cell Biology 2020Quote: ... Transfections and co-transfections were performed using FuGENE 6 transfection reagent (Promega). Cells were analysed two days post-transfection.
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bioRxiv - Genetics 2022Quote: ... was transiently transfected with the pRL-TK Renilla internal control vector (50 ng) using 4 μL Viafect™ transfection reagent (Promega) into suspension cell lines or adherent cell lines ...
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bioRxiv - Developmental Biology 2021Quote: ... or experimental constructs along with the ptcΔ136-GL3 luciferase reporter construct and beta-galactosidase transfection control (pSV-β-galactosidase; Promega, E1081).
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bioRxiv - Cell Biology 2019Quote: ... We then assessed cell viability of treated and control cells 48 hours post-transfection using the CellTiter Glo 2.0 luciferase assay kit (Promega, Madison, WI) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2019Quote: ... HEK293T cells were transfected with control or shRNA-containing pFUGW (GFP-expressing) vector plasmids and helper plasmids PAX2 and VSVG using Fugene (Promega). Supernatant was collected 65 hr after transfection ...
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bioRxiv - Molecular Biology 2020Quote: ... (49) as a negative control (2 μg of DNA per well of 6 well plate) with FuGene transfection reagent (Promega, Madison, WI) according to manufacturer’s instructions.
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bioRxiv - Developmental Biology 2019Quote: ... 10ng of hRLuc pGL4.75 control vector in 24-well plate format using FuGENE® HD transfection reagent (Promega Corporation, Wisconsin, United States). The cells were maintained in incomplete media in 25°C incubator for 8 hours after transfection and were then maintained in complete media for 48 hours ...
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bioRxiv - Microbiology 2021Quote: ... and the Firefly control plasmid (pGL3-Control; Promega). After plasmids were added to the Opti-MEM ...
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bioRxiv - Plant Biology 2023Quote: ... Plasmid pFL61 harbouring the construct RiSKC3-GFP under the control of the PGK promoter was purified from StellarTM bacteria using the PureYield™ Plasmid Miniprep System (Promega). The yeast transformation protocol was described by (50).
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bioRxiv - Microbiology 2021Quote: ... HepG2 or sg25 cells were transfected with a control plasmid or protein expression plasmids together with the luciferase reporter plasmids using Viafect TM Transfection Reagent (Promega, Madison, WI, USA) or Lipofectamine TM 2000 (Invitrogen ...
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bioRxiv - Developmental Biology 2021Quote: ... cells were transfected pCAG-Lgr6 or control plasmid (100 ng/ml or as noted) using the Fugene HD transfection reagent (Promega, Madison, WI, USA). Subsequent cell culturing and treatment is as noted for individual assays ...
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bioRxiv - Biochemistry 2024Quote: ... Kc167 cells were seeded in 6-well plates and transfected (3 µg DNA per well) with the JHRE reporter or control (EGFP-pIEx-4) plasmids using the standard FuGENE-HD transfection protocol (Promega, Madison, WI, USA). About 48 h post-transfection ...
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bioRxiv - Developmental Biology 2021Quote: ... Transfection was performed with FuGENE 6 transfection reagent (Promega). All the cell lines were regularly confirmed to be free of contamination (e.g ...
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bioRxiv - Biophysics 2020Quote: ... and transfections were performed using Fugene transfection reagent (Promega). The transfected Lenti-X 293T cells were incubated at 37° with 5% CO2 for two days ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Transfections were done using Fugene HD transfection agent (Promega) at ratio 1:4 (w/v ...
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bioRxiv - Neuroscience 2022Quote: ... Transfections were done using the FuGENE6 Transfection Reagent (Promega). Transfected cells were incubated at 37°C with 95% air and 5% CO2 overnight for use in whole-cell recordings ...
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bioRxiv - Cell Biology 2024Quote: ... Cell transfection was performed using FuGene transfection reagents (Promega) as recommended by the manufacturer ...
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bioRxiv - Cell Biology 2020Quote: ... The AIO plasmid and the template plasmid were transfected into HAP1 STG-p62 (for mScarlet-AID-NBR1) or HAP1 WT (for GFP-AID-NBR1) cells using FuGene 6 transfection reagent (Promega, cat# e2691). 48h later the cells were sorted for mScarlet fluorescence in bulk ...
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bioRxiv - Cancer Biology 2019Quote: ... Cells were co-transfected with pCAGGS-FLPe and col1a1-EF1α-GFP at a 1:2 ratio using FuGENE HD Transfection Reagent (Promega, Cat. # E2311), and selected in 50μg/ml hygromycin starting 48 hours post-transfection ...
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bioRxiv - Molecular Biology 2022Quote: ... the cells were transfected with 1 μg of DNA (1 μg of an L1-expressing plasmid or 0.5 μg of the L1-expressing plasmid and 0.5 μg of either a pCMV-3Tag-8-Barr control or ISG-expressing plasmid) using 3 µL of FuGENE HD transfection reagent (Promega, Madison, WI, United States) and 100 µL of Opti-MEM (Gibco ...