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Citations for Promega :
51 - 100 of 5335 citations for hsa mir 185 Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2019Quote: A wild-type or mut-hsa-miR-329-5p fragment was constructed and inserted downstream of the luciferase reporter gene of the pmirGLO Vector (Promega, USA). We used ExFect 2000 Transfection Reagent to transfect the reporter plasmid into 293T cells ...
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bioRxiv - Cell Biology 2022Quote: ... Quantitative real time-PCR was performed using GoTaq® qPCR Master Mix (Promega) in a QuantStudio 6 Flex thermocycler (Applied Biosystem ...
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bioRxiv - Cancer Biology 2022Quote: ... and analyzed by SYBRG real-time PCR using GoTaq qPCR Master Mix (Promega). Primers used are provided in supplementary table S3.
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bioRxiv - Microbiology 2020Quote: ... Semi-quantitative real-time PCR was performed using GoTaq qPCR Master Mix (Promega) using primers targeting the SARS-CoV-2 E protein gene (forward primer ...
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bioRxiv - Developmental Biology 2022Quote: ... Quantitative real time-PCR was performed using Go-Taq qPCR Master Mix (Promega) in a QuantStudio 6 Flex thermocycler (Applied Biosystem) ...
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bioRxiv - Immunology 2020Quote: ... Samples were analyzed by real-time PCR with GoTaq qPCR Master Mix (Promega) on the Applied Biosystem 7900HT Fast machine ...
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bioRxiv - Plant Biology 2023Quote: ... Real-time PCR was performed using Gotaq® qPCR mastermix (Promega, Madison, WI) and a CFX 96 real-time system (Bio-Rad) ...
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bioRxiv - Cancer Biology 2023Quote: ... and analysed by SYBRG real-time PCR using GoTaq qPCR Master Mix (Promega). Primers used are provided in supplementary Table S2.
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bioRxiv - Cell Biology 2020Quote: ... Real-time PCR was performed using the GoTaq qPCR Master Mix (Promega, Cat. M3001) in LightCycler® 480-Roche System according to the supplier’s manual ...
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bioRxiv - Molecular Biology 2019Quote: ... Quantitative real-time PCR (qPCR) was carried out using GoTaq qPCR master mix (Promega) on CFX96 Real-Time PCR Detection System (Bio-Rad) ...
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bioRxiv - Developmental Biology 2019Quote: ... Quantitative real-time PCR (qPCR) assays were performed using GoTaq qPCR Master Mix (Promega) on a ViiA 7 Real-Time PCR System according to manufacturer’s instructions (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2021Quote: ... qRT-PCRs were performed with the SYBR-Green Real Time Master Mix (Promega; A600A) according to manufacturer’s instructions using a BioRad CFX96 machine ...
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bioRxiv - Neuroscience 2021Quote: ... Quantitative real time PCR was carried out using the GoTaq qPCR Master Mix (Promega) and LC480 detection system (Roche) ...
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bioRxiv - Microbiology 2021Quote: ... real-time quantitative PCR using the GoTaq qPCR Master Mix (Promega, Madison, WI, USA) was performed targeting a 158 bp stretch of the sodB gene using primers specific for the symbiont (sodB-F ...
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bioRxiv - Zoology 2024Quote: RT-PCR was performed using AccessQuick RT-PCR System (Promega, France) in total reaction volume of 25 µl with 5 µl of RNA extracts and 400 nM of forward primer (5’-ATTCGGGAGGAGACGTGGTA-3’ ...
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bioRxiv - Microbiology 2021Quote: ... and real-time PCR was performed on 30 ng of DNA with SYBR Green kit GoTaq® qPCR Master Mix (Promega). DNA from non-transduced cell was isolated and amplified at the same time to demonstrate the absence of contamination ...
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bioRxiv - Cancer Biology 2019Quote: ... RT-PCR was performed using the Access RT-PCR System from Promega. Real-time PCR was done using SYBR Green Realtime PCR Master Mix (TOYOBO ...
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bioRxiv - Cancer Biology 2019Quote: ... RT-PCR was performed using AccessQuick™ RT-PCR system (Promega #A1700) and samples were ran on 1.2% agarose gel ...
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bioRxiv - Physiology 2023Quote: ... The obtained cDNA was used as the template for quantitative real-time PCR (qRT-PCR) using qPCR Mastermix (Promega). mRNAs expression levels were normalized to actin-5C mRNA used as the internal control The relative level for each gene was calculated using the 2-DDCt method (Hulf et al. ...
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bioRxiv - Cell Biology 2023Quote: ... The obtained cDNA was used as the template for quantitative real-time PCR (qRT-PCR) using qPCR Mastermix (Promega). mRNAs expression levels were normalized to actin-5C mRNA used as the internal control ...
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bioRxiv - Cancer Biology 2019Quote: ... Real-time PCR reactions were carried out using 1x GoTaq™ qPCR Master Mix (Promega) and 0,25 μM of forward and reverse primers (Table S5 ...
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bioRxiv - Microbiology 2020Quote: ... quantitative real-time PCR was carried out according to the GoTaq qPCR Master Mix (Promega) with 20 µl of a reaction mixture containing gene-specific primers (Supplementary Table 3) ...
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bioRxiv - Physiology 2024Quote: ... Real-time PCR reaction was performed with the GOTaq® qPCR Master Mix (A6001, Promega) and CFX96 Real-Time PCR Detection System (Bio-Rad) ...
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bioRxiv - Genetics 2022Quote: Chromatin accessibility by real-time PCR (ChART-PCR) was performed as previously described (75) using 20 U DNase I (Promega). To assess nucleosome occupancy ...
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bioRxiv - Molecular Biology 2021Quote: ... Viral RNA of SARS-COV-2 was detected by TaqMan®-based Real-Time PCR (Promega) using a CDC protocol with two sets of primer/probe which amplifies virus nucleocapsid (N ...
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bioRxiv - Cancer Biology 2023Quote: ... Then prepared cDNA was subjected to real-time PCR analysis with Eastep qPCR Master Mix (Promega) and primer mixtures (primer sequences are shown in table S2) ...
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bioRxiv - Microbiology 2023Quote: ... Quantitative real-time PCR was performed using the 2x GoTaq qPCR master mix (Promega, Walldorf, Germany) with 10 ng of cDNA template and 0.5 µM of each oligonucleotide in a final volume of 15 µL ...
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Phosphoproteomics of cellular mechanosensing reveals NFATC4 as a regulator of myofibroblast activitybioRxiv - Systems Biology 2023Quote: ... Quantitative PCR (qPCR) was then performed on LightCycler 480 Real-Time PCR System using the GoTaq qPCR Master Mix (Promega, #A6001), and the primers pairs are listed in Table S7 ...
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bioRxiv - Molecular Biology 2024Quote: ... and gene expression was determined by quantitative PCR (QuantStudio 6 Pro Real-Time PCR System) with GoTag qPCR System (Promega, A6002). Actb and Rplp0 were used as housekeeping genes ...
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bioRxiv - Cell Biology 2023Quote: ... Quantitative Realtime PCR (qRT-PCR) was performed using GoTaq® 1-Step RT-qPCR kit (Promega) with primers specific for PEX1 (RE7039-RE7040 ...
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bioRxiv - Cell Biology 2019Quote: ... and analyzed by analytical RT-PCR using the GoTaq Green kit (Promega) with primers 1171 and 1174 (see Key Resources Table) ...
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bioRxiv - Immunology 2020Quote: ... Bacterial groups in cecal samples were measured by real-time PCR using GoTaq qPCR Master Mix (Promega) on Applied Biosystem 7900HT Fast or StepOne Plus™ Real-Time PCR Systems ...
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bioRxiv - Cancer Biology 2023Quote: ... Then the eluted DNA was subjected to real-time PCR analysis with Eastep qPCR Master Mix (Promega) and promoter-specific primer mixtures ...
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bioRxiv - Plant Biology 2021Quote: ... Reverse transcription (RT) involved the Access RT-PCR System (Promega), as per the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... AtLPAAT4 and AtLPAAT5 by RT-qPCRs were performed with the Bio-Rad CFX96 real-time system using GoTaq® qPCR Master mix (Promega # A6002). The specific primer pairs used for AtLPAAT2 ...
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bioRxiv - Microbiology 2019Quote: ... Quantitative real-time polymerase chain reaction (PCR) was performed for CGRP with GoTaq® qPCR Master Mix (Promega) using the DNA-binding dye ...
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bioRxiv - Molecular Biology 2020Quote: ... Quantitative real time PCR (qPCR) from 10ng of gDNA was performed using GoTaq qPCR Master Mix (Promega, A6001) on a TaqMan Viia 7 Real-Time PCR System ...
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bioRxiv - Immunology 2022Quote: ... MCMV-DNA was quantified by real-time PCR using BIO-RAD CFX with GoTaq qPCR Master Mix (Promega) and primers specific for MCMV glycoprotein B (gB ...
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Mitochondrial Apolipoprotein MIC26 is a metabolic rheostat regulating central cellular fuel pathwaysbioRxiv - Cell Biology 2023Quote: ... quantitative real-time PCR was performed in Rotor Gene 6000 (Corbett Research) using GoTagR qPCR Master Mix (Promega) according to manufacturer’s instructions with the following primers:
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bioRxiv - Developmental Biology 2021Quote: PCR (RT-PCR) was conducted using GoTaq DNA polymerase (Promega). PCR programs were the following ...
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bioRxiv - Molecular Biology 2021Quote: ... qPCR was performed on a Bio-Rad CFX96 Real-Time PCR system with the GoTaq qPCR Master Mix (Promega). Relative gene expression levels were calculated using the ΔΔCt method with CPA as the reference gene.
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CASC3 promotes transcriptome-wide activation of nonsense-mediated decay by the exon junction complexbioRxiv - Molecular Biology 2020Quote: ... Quantitative real time PCR was performed with 16 ng of cDNA per reaction with GoTaq qPCR Master Mix (Promega) and the CFX96 Touch Real-Time PCR Detection System (Biorad) ...
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bioRxiv - Cell Biology 2021Quote: The viral titers were determined by quantitative real-time PCR using GoTaq® qPCR Master Mix (Promega, Madison, WI) on a CFX96 Touch™ Real-Time PCR Detection System (Bio-Rad ...
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bioRxiv - Cancer Biology 2020Quote: ... cDNA was diluted 1:5 in nuclease free water and 2μl of the diluted product was used for real time PCR with the following reagents: 5μl of GoTaq qPCR Master Mix (Promega), 2μl of nuclease free water and 1μl of 10mM primer mix ...
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bioRxiv - Biophysics 2021Quote: ... using the Access RT-PCR System (Promega), and then exchanged with exon 6b in mH1 using the Quikchange method of site-directed mutagenesis (SDM ...
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bioRxiv - Cancer Biology 2022Quote: ... These cells were transfected with the pcDNA6.2-GW /EmGFPmiR plasmids containing miR sequences (miR-NRF2 #1, #2, #3, #4 and miR-ctl 79 using the FUGENE HD transfection reagent (Promega #E2311). Seven days later GFP positive cells were isolated by flow cytometry (Biorad S3e cell sorter) ...
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bioRxiv - Molecular Biology 2020Quote: ... and real-time polymerase chain reaction (RT-PCR) conducted using the Techne Prime Pro thermal cycler with GoTaq qPCR Master Mix (Promega). RT-qPCR reactions were carried out in 10 μL volumes containing 3.5 mM MgCl2 ...
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bioRxiv - Plant Biology 2020Quote: ... Quantitative real time RT-PCR (qPCR) was performed using gene-specific primers (Supplemental Table 1) and the fluorescent dye SYBR Green (Promega). All quantifications were normalized to the TIP41 (AT4G34270 ...
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bioRxiv - Developmental Biology 2021Quote: ... probes with GoTaq Real Time qPCR Master Mix (Promega) were used ...
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bioRxiv - Microbiology 2020Quote: ... Real Time qPCR was performed using GoTaq polymerase (Promega) and the StepOnePlus real-time PCR thermocycler (ThermoFisher ...