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Citations for Promega :
651 - 700 of 2430 citations for hsa mir 150 3p RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2020Quote: ... The PCR was performed using the GoTaq® Long PCR MasterMix (Promega, Charbonnieres, France) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: ... PCR products were purified using the PCR Promega kit (Promega Corporation, WI, États-Unis) following the manufacturer’s instructions and sent to Genewiz company (www.genewiz.com/en-GB ...
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bioRxiv - Microbiology 2023Quote: ... PCR samples were purified by the Wizard SV Gel and PCR Clean system (Promega) following manufacturers’ instructions ...
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bioRxiv - Evolutionary Biology 2023Quote: ... The PCR reaction was carried out using GoTaq Long PCR 2x Master Mix (Promega) for 35 cycles ...
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bioRxiv - Microbiology 2023Quote: ... PCR fragments from the nested PCR were cloned into pGEM-T-easy vectors (Promega), and the indicated number of successful recombinant clones was randomly selected and sequenced using a 3500 Genetic Analyzer (Thermo Fisher Scientific).
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bioRxiv - Genomics 2023Quote: ... 20 ml PCR reactions contained 10 ml GoTaq® green PCR Master mix (Promega), 6 ml nuclease-free water ...
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bioRxiv - Plant Biology 2024Quote: ... followed by PCR amplification across the CRISPR target sequences with DreamTaq PCR reagent (Promega) and Sanger sequencing (Eurofins genomics) ...
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bioRxiv - Evolutionary Biology 2021Quote: ... University of Connecticut) with V4 16S rRNA primers as above using GoTaq DNA Polymerase (PROMEGA).
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bioRxiv - Microbiology 2021Quote: ... and reverse transcribed with random primer combined with Im-Prom Reverse Transcription System (Promega #A3800). Quantitative real-time PCR was performed using FastStart Universal SYBR Green Master Mix (Roche #04913850001 ...
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bioRxiv - Cancer Biology 2021Quote: ... 1 μg of purified RNA was dissolved with 0.5 μg of oligo (dT) primer (Promega), 0.5 mM dNTPs ...
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bioRxiv - Plant Biology 2021Quote: ... each primer at 0.25 μM and 5 μL of 2X GoTaq qPCR Master Mix (Promega). The amplification program consisted of preincubation steps at 50°C for 2 min and 95°C for 10 min followed by 40 cycles of 95°C for 15 s and 60°C for 1 min ...
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bioRxiv - Cell Biology 2020Quote: ... Complementary DNA was synthesized using 1.5 μg of RNA with random Oligo-dT-Primers (Promega) and M-MLV Reverse Transcriptase (Promega ...
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bioRxiv - Evolutionary Biology 2022Quote: ... primers (0.4μM) and 0.75U of Go Taq® DNA polymerase enzyme (Promega Madison, Wisconsin, USA) with its buffer (1x) ...
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bioRxiv - Molecular Biology 2022Quote: ... 1 μL 10xcDNA and 0.3 μL of Forward and Reverse primers as recommended by Promega Corporation Super Real Premix ...
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bioRxiv - Microbiology 2019Quote: ... with primers specified in Supplementary Table 1 and cloned into vector pGEM-T Easy (Promega). A number of resulting clones was Sanger-sequenced and analyzed using the BLAST tool at PlasmoDB (www.plasmodb.org) ...
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bioRxiv - Cancer Biology 2020Quote: ... RNA was reverse transcribed into cDNA using random hexanucleotide primers and M-MLV enzyme (Promega). ChIP experiments were performed using 20μg anti-ΔNp63 (Biolegend ...
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bioRxiv - Cancer Biology 2021Quote: ... RNA was reverse transcribed into cDNA using random hexanucleotide primers and M-MLV enzyme (Promega). Quantitative RT-PCR was performed with SYBR Green mix (ABgene ...
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bioRxiv - Developmental Biology 2020Quote: ... RNA was reverse-transcribed to cDNA using oligo(dT)18 primer and M-MLV (Promega). qPCR was performed using SYBR Green (Bio-Rad ...
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bioRxiv - Genomics 2021Quote: ... cDNA was synthesised from 1 μg of total RNA using random primers (Promega, Wisconsin, USA) then treated with M-MLV Reverse Transcriptase (Pro-mega ...
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bioRxiv - Microbiology 2022Quote: ... 50 pM of each primer 2U of GoTaq® DNA polymerase (Promega, Madison, WI, USA) and 100 ng of DNA template ...
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bioRxiv - Plant Biology 2022Quote: ... according to the instructions with the following modifications: stock oligo(dT)15 primers (C1101, Promega) concentration was 50 ng/μl and 0.5 μl Superscript II enzyme was used per reaction ...
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Genetic characterization of the zoonotic parasite Ancylostoma caninum in the central and eastern USAbioRxiv - Microbiology 2022Quote: ... 200nM of each primer and 0.3 units of Taq polymerase (GoTaq Flexi, Promega, Madison, WI). PCR conditions were 94°C for 5 minutes followed by 30 cycles of 94°C for 30s ...
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bioRxiv - Cell Biology 2022Quote: ... and DNase-I digested before murine leukemia virus (MLV) reverse transcription using random primers (Promega). qPCR was performed using the TaqMan technology (Life Technologies) ...
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bioRxiv - Bioengineering 2024Quote: ... RNA was reverse transcribed to cDNA using random primers and GoScript Reverse Transcriptase kit (Promega). RT-qPCR was performed using Firepol qPCR Master Mix (Solis BioDyne ...
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bioRxiv - Pathology 2024Quote: ... following the manufacturer’s instructions and using the optional RNAse H digestion and oligodT15 primers (Promega). qRT PCR was performed with the LUNA Universal qPCR Master Mix (NEB ...
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bioRxiv - Immunology 2020Quote: ... pCAG-Flag or pCMV-HA-N expression vectors using standard molecular cloning methods as described in our previous publications.30–32 The IFN-β luciferase reporter plasmid pGL3-IFN-β-Luc vector was constructed in our previous study.33,34 The IFNλ1 luciferase reporter plasmid pGL3-IFNλ1-Luc was constructed by inserting the 1000-bp promoter region of human IFNλ1 (nucleotides −1000 to +1, with the translation start site set as 1) into pGL3-Basic (Promega, USA) according to methods outlined in previous studies.13,34 The ISG luciferase reporter plasmid pISRE-Luc vector was purchased from Clontech (USA) ...
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bioRxiv - Cancer Biology 2023Quote: ... In-solution digestion was performed overnight on a shaker set at 37 °C using sequencing grade trypsin (Promega, Madison, WI) at ∼1:25 w/w enzyme/protein ratio ...
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bioRxiv - Microbiology 2019Quote: PCR (GoTaq®Green, Promega) was performed on gDNA using primers listed in Suppl ...
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bioRxiv - Bioengineering 2021Quote: ... PCR product was purified (Promega) and digested using DpnI (NEB ...
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bioRxiv - Neuroscience 2020Quote: ... PCR Nucleotide Mix (Promega, UK) and GoTaq® Flexi DNA Polymerase (Promega ...
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bioRxiv - Cell Biology 2020Quote: ... PCR nucleotide mix (Promega: U144B) and oligo (dT ...
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bioRxiv - Genetics 2020Quote: ... PCR Master Mix 2x (Promega) was used ...
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bioRxiv - Biochemistry 2022Quote: ... and PCR with GoTaq (Promega) using the primers-RG6 S ...
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bioRxiv - Cell Biology 2022Quote: ... 2.0 ug DNA of expression plasmids were mixed 150 μL Opti-MEM and transfected using FuGENE HD (Promega) at a DNA to FuGENE HD ratio of (1:3) ...
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bioRxiv - Physiology 2019Quote: ... and this was followed by an intraperitoneal injection of 150 mg/kg body weight of luciferin (VivoGlo; Promega). Luciferase activity was monitored 10 minutes after the luciferin injection.
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bioRxiv - Genomics 2023Quote: ... song: 150 μg total) was quantified using the Quantus Fluorometer (QuantiFluor ONE dsDNA Dye assay; Promega, Madison, WI). The size distribution of the HMW DNA was estimated using the Femto Pulse system (Agilent ...
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bioRxiv - Microbiology 2023Quote: ... Cells were then unfixed overnight in 150 mM NaCL and 60C and 50 ug/mL protease K (Promega). DNA was then extracted using a single column of QIAamp DNA Blood Mini Kit (Qiagen) ...
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bioRxiv - Genetics 2021Quote: ... PCR products were purified with the Wizard SV Gel and PCR Clean-Up System (Promega) and quantified with a Qubit 2.0 Fluorometer (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2021Quote: ... PCR products were then purified using Wizard SV Gel and PCR Clean-Up System (Promega) according to the instructions by the manufacturer ...
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bioRxiv - Cancer Biology 2022Quote: ... PCR products were cleaned with the Wizard SV Gel and PCR Clean Up Kit (Promega) and cut with XhoI and BamHI or HindIII und BamHI (Thermo Scientific) ...
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bioRxiv - Microbiology 2022Quote: ... PCR products were purified by the Wizard SV Gel and PCR Clean-Up System (Promega) and quantified using the Qubit dsDNA HS assay (ThermoFisher) ...
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bioRxiv - Microbiology 2021Quote: ... PCR products were purified with Wizard® SV Gel and PCR Clean-Up System (Promega) and ligated to the pGEM-T easy® vector (Promega) ...
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bioRxiv - Molecular Biology 2019Quote: ... PCR products were purified using a Wizard SV Gel and PCR Clean-Up System (Promega) and forward and reverse sequences were obtained using an Abi 3130XL Genetic Analyzer (Applied Biosystem ...
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bioRxiv - Microbiology 2019Quote: ... PCR products were purified using the Wizard SV Gel and PCR Clean-up System (Promega). The concentrations of amplified PCR products were measured using the Quant-iT PicoGreen dsDNA Assay Kit (Molecular Probes ...
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bioRxiv - Bioengineering 2021Quote: ... Input linear DNA was produced by PCR and cleaned with PCR cleanup kit (Promega, A6754), quantified and diluted to desired concentrations using ultra-pure water ...
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bioRxiv - Molecular Biology 2022Quote: ... The PCR products were purified with Wizard SV Gel and PCR Clean-Up System (Promega). A volume of 0.75 µl of the DNA templates was mixed in 2.5 µl cell-free protein expression reactions (PUREFrex 2.0 ...
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bioRxiv - Cancer Biology 2023Quote: ... PCR cleanup was conducted using the Wizard SV Gel and PCR Clean-Up System (Promega) with elution in 30 μl of double distilled water ...
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bioRxiv - Immunology 2024Quote: The PCR products representing genomic and cDNA sequences were purified using PCR purification kit (Promega) and subsequently sequenced using Sanger sequencing at Micromon Genomics ...
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bioRxiv - Genetics 2023Quote: ... PCR products were purified using the Wizard SV Gel and PCR Clean-Up System (Promega) in line with the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2020Quote: ... Each quantitative RT-qPCR reaction was performed using the GoTaq qPCR Master Mix kit (Promega). For a 10 μl reaction ...